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| 1 | Reactive capillary hemangiomas: a novel dermatologic toxicity following anti-PD-1 treatment with SHR-1210显示文摘Objective: SHR-1210 is a new and promising anti-PD-1 agent for solid tumors. During the phase I study of SHR-1210, we encountered a novel but prevalent immune-related dermatologic toxicity: reactive capillary hemangiomas(RCHs). Thus we tried to summarize the features of RCHs and estimate their relationship with tumor response.Methods: This prospective observational study systematically enrolled 98 patients with advanced solid tumors from April 27th,2016 to June 8th, 2017 in the context of the phase I clinical study of SHR-1210. This report focused on the skin toxicities. Patients underwent entire skin inspection every two weeks while taking medication. The clinical course of RCHs was recorded and their association with tumor response was estimated. The data cut-off date was November 15th, 2017.Results: After a median follow-up of 242(range, 29–567) days, RCHs were observed in 85.7%(84/98) of patients on cutaneous/mucosal surfaces; 84.5%(71/84) of the RCHs were evaluated as grade 1 adverse events. No grade 3 or 4 RCHs were observed. The time of onset of RCHs was dose dependent and shortest in the 400 mg-dose cohort(P < 0.001). Spontaneous and complete regression of RCHs was observed both during and after treatment. The objective response rate of tumors for patients with RCHs was 28.9%(24/83). However, no responders were observed among the patients without RCHs.Conclusions: RCHs were prevalent but manageable during treatment with SHR-1210. It might add to the expanding literature regarding immune-related dermatologic adverse events. | Xuelian Chen Lanying Ma Xi Wang Hongnan Mo Dawei Wu Bo Lan Dong Qu Hongtu Zhang Jing Huang Binghe Xu | 2019 | Cancer Biology & Medicine2019，16，1： | 53 |
| 2 | Phase II clinical trial using camrelizumab combined with apatinib and chemotherapy as the first-line treatment of advanced esophageal squamous cell carcinoma显示文摘Background:Effective therapeutic options are limited for patients with advanced esophageal squamous cell carcinoma(ESCC).The incorporation of an immune checkpoint inhibitor and a molecular anti-angiogenic agent into the commonly adopted chemotherapy may produce synergistic effects.Therefore,we aimed to investigate the efficacy and safety of camrelizumab plus apatinib combined with chemotherapy as the first-line treatment of advanced ESCC.Methods:In this single-arm prospective phase II trial,patients with unresectable locally advanced or recurrent/metastatic ESCC received camrelizumab 200 mg,liposomal paclitaxel 150 mg/m2,and nedaplatin 50 mg/m2 on day 1,and apatinib 250 mg on days 1-14.The treatments were repeated every 14 days for up to 9 cycles,followed by maintenance therapy with camrelizumab and apatinib.The primary endpoint was objective response rate(ORR)according to the Response Evaluation Criteria in Solid Tumors(version 1.1).Secondary endpoints included disease control rate(DCR),progression-free survival(PFS),overall survival(OS),and safety.Results:We enrolled 30 patients between August 7,2018 and February 23,2019.The median follow-up was 24.98 months(95%confidence interval[CI]:23.05-26.16 months).The centrally assessed ORR was 80.0%(95%CI:61.4%-92.3%),with a median duration of response of 9.77 months(range:1.54 to 24.82+months).The DCR reached 96.7%(95%CI:82.8%-99.9%).The median PFS was 6.85 months(95%CI:4.46-14.20 months),and the median OS was 19.43 months(95%CI:9.93 months–not reached).The most common grade 3-4 treatmentrelated adverse events(AEs)were leukopenia(83.3%),neutropenia(60.0%),and increased aspartate aminotransferase level(26.7%).Treatment-related serious AEs included febrile neutropenia,leukopenia,and anorexia in one patient(3.3%),and single cases of increased blood bilirubin level(3.3%)and toxic epidermal necrolysis(3.3%).No treatment-related deaths occurred.Conclusions:Camrelizumab plus apatinib combined with liposomal paclitaxel and nedaplatin as first-line treatment demonstrated feasible anti-tumor activity and manageable safety in patients with advanced ESCC.Randomized trials to evaluate this new combination strategy are warranted.Trial registration:This trial was registered on July 27,2018,at ClinicalTrials.gov(identifier:NCT03603756). | Bo Zhang Ling Qi Xi Wang Jianping Xu Yun Liu Lan Mu Xingyuan Wang Lidan Bai Jing Huang | 2020 | Cancer Communications2020，40，12： | 38 |
| 3 | Effects of Short-Term Forest Bathing on Human Health in a Broad-Leaved Evergreen Forest in Zhejiang Province,China显示文摘Objective To investigate the effects of short-term forest bathing on human health.Methods Twenty healthy male university students participated as subjects and were randomly divided into two groups of 10.One group was sent on a two-night trip to a broad-leaved evergreen forest,and the other was sent to a city area.Serum cytokine levels reflecting inflammatory and stress response,indicators reflecting oxidative stress,the distribution of leukocyte subsets,and plasma endothelin-1 (ET-1) concentrations were measured before and after the experiment to evaluate the positive health effects of forest environments.A profile of mood states (POMS) evaluation was used to assess changes in mood states.Results No significant differences in the baseline values of the indicators were observed between the two groups before the experiment.Subjects exposed to the forest environment showed reduced oxidative stress and pro-inflammatory level,as evidenced by decreased malondialdehyde,interleukin-6,and tumor necrosis factor α levels compared with the urban group.Serum cortisol levels were also lower than in the urban group.Notably,the concentration of plasma ET-1 was much lower in subjects exposed to the forest environment.The POMS evaluation showed that after exposure to the forest environment,subjects had lower scores in the negative subscales,and the score for vigor was increased.Conclusion Forest bathing is beneficial to human health,perhaps through preventive effects related to several pathological factors. | MAO Gen Xiang LAN Xiao Guang CAO Yong Bao CHEN Zhuo Mei HE Zhi Huat LV Yuan Dong WANG Ya Zhen HU Xi Lian WANG Guo Fu YAN Jing | 2012 | Biomedical and Environmental Sciences2012，25，3： | 20 |
| 4 | Effect of L-NAME on nitric oxide and gastrointestinal motility alterations in cirrhotic rats显示文摘AIM: To invsstigare the effect of L-NAME on nitric oxide andgastriubtestubal motility alterations in cirrhotic ratsMETHODS: Rats with cirrhosis induced by carbontetrachloride were randomly divided into two groups, one( n= 13) receiving 0. 5 mg@ kg-1 per clay of NG-nitro-L-argininemethyl ester (L-NAME), a nitric oxide synthase inhibitor,for 10 days, whereas the other group ( n = 13) and control( n = 10) rats were administrated the same volume of 9 g@ L-1saline.Half gastric emptying time and 2 h residual rate weremeasured by SPECT, using 99m Tc-DTPA-labeled bariumsuifate as test meal. Gastrointestinal transition time wasrecorded simultaneously. Serum concentration of nitrcoxide (NO) was determined by the kinetic cadmiunreduction and colorimetric methods. ImmunohistochemicalSABC method was used to observe the expression anddistribution of three types of nitric oxide synthase (NOS)isoforms in the mt gastrointestinal tract. Western blot wasused to detect expression of gastrointestinal NOS isoforms.RESULTS: Half gastric emptying time and trans-gastrointestinal time were significantly prolonged( 124.0 ± 26.4min; 33.7± 8.9min;72.1 ± 15.3 min; P<0.01), (12.4±0.5h; 9.5±0.3 h; 8.2±0.8 h; P<0.01), 2h residual rate wasraised in cirrhotic rots than in controls and cirrhotic ratstreated with L-NAME(54.9± 7.6 % ,13.7 ± 3.2 %, 34.9± 10.3%, P< 0.01). Serum concentration of NO was significantlyincreased in cirrhotic rots than in the other groups (8.20 ± 2.48)μmol@L-1, (5.94± 1.07) μmol@L-1 ,and control (5.66± 1.60) tμmol@L-1, P< 0.01. NOS staining intensities which weremainly located in the gastrointestinal tissues were markedlylower in cirrhotic rats than in the controls and cirrhotic ratsafter treated with L- NAME.CONCLUSION: Gastrointestinal motility was remarkablyinhibited in cirrhotic rats, which could he alleviated by L-NAME. Nitric oxide may play an important role in theinhibition of gastrointestinal motility in cirrhotic rats. | Xin Wang Zong-You Zhang Mei Lan Ji-Yan Miao Xue-Gang Guo Yong-Quan Shi Yan-Qiu Zhao Jie Ding Kai-Cun Wu Dai-Ming Fan,Institute of Digestive disease,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China Yue-Xia Zhong,Emergency Department,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038,Shaanxi Province,China Ju Lu,Class EE 87,Department of Electronic Engineering,Tsinghua University,Beijing 100084,China Bo-Rong Pan,Oncology Center,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 19 |
| 5 | Effect of the number of freeze-thaw cycles number on the quality of Pacific white shrimp (Litopenaeus vannamei): An emphasis on moisture migration and microstructure by LF-NMR and SEM显示文摘The objective of this study was to evaluate the effects of the number of freeze-thaw(F-T)cycles on moisture migration,protein degradation,microstructure and quality in Litopenaeus vannamei.The quality of samples with different F-T cycles were determined by low-field nuclear magnetic resonance(LF-NMR),magnetic resonance imaging(MRI)and scanning electron microscopy(SEM),combined with sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE),texture properties analysis(TPA),color difference,polyphenol oxidase(PPO)activity,total volatile basic nitrogen(TVB-N),total viable count(TVC),and sensory evaluation.The results showed that F-T cycles caused a significantly increase in transverse relaxation time in T22 and T23 and a decreased brightness of pseudo-color diagram after 4 F-T cycles,indicating that water mobility increased as immobilized water was shifted to free water.The texture of samples declined as well,especially after 4 F-T cycles.The rapid growth of PPO activity caused a decrease in brightness(L*)and an increase in redness(a∗)after 3 F-T cycles.The increase of TVB-N,TVC,and sensory score explained the changes in shrimp quality which became obvious after 3 F-T cycles and unacceptable after 6 cycles.Meanwhile,fewer than 4 F-T cycles accelerated protein aggregation,while denaturation occurred after 4 cycles.Therefore,repeated F-T cycles could accelerate the changes of protein,microstructure,water distribution,and quality deterioration especially after 3 F-T cycles,with a threshold was 6 F-T cycles. | Weiqing Lan Xiaoyu Hu Xiaohong Sun Xi Zhang Jing Xie | 2020 | Aquaculture and Fisheries2020，5，4： | 15 |
| 6 | Synthesis and Characterization of Phosphated Konjac Glucomannan Hydrogels显示文摘Konjac glucomannan (KGM) was crosslinked with sodium tripolyphosphate (STPP) to synthesize hydrogels. The crosslinking reaction was confirmed by FT-IR. The results of degradation test show that the hydrogels retain the enzymatic degradation character of KGM and can be degraded for 74.45% in 5 days by cellulase E0240. | Li Gui CHEN Zhi Lan LIU Ying Jun CHEN Ren Xi ZHUO | 2005 | Chinese Chemical Letters2005，16，12： | 13 |
| 7 | Target classification with low-resolution radar based on dispersion situations of eigenvalue spectra显示文摘Most low-resolution radar systems, especially ground surveillance radar systems, work at relatively low pulse repeat frequency (PRF) and with short time-on-target (TOT) (duration in scanning). Low PRF leads to Doppler ambiguity and short TOT results in low Doppler resolution, which poses a problem to target classification with low-resolution radar based on the jet engine modulation (JEM) characteristic of radar echo. From the pattern classification viewpoint, we propose a method of using dispersion situations of JEM eigenvalue spectra to categorize aeroplanes into three kinds, namely turbojet aircraft, prop aircraft and helicopter. We analyze the mathematical model of JEM echoes consisting of a series of line spectra and regard them as a sum of several series of harmonious waves. Classification features can be extracted based on the harmonious wave sum model. Some schemes for extracting features from echoes within or between pulses are proposed. Low-dimensional features are extracted to reduce computation burden. Our methods do not compensate for the fuselage echoes and are insensitive to the variation of fuselage Doppler. The feasibility of our methods is demonstrated by simulation experiment. | CHEN Feng, LIU HongWei, DU Lan & BAO Zheng National Laboratory of Radar Signal Processing, Xidian University, Xi’an 710071, China | 2010 | Science China(Information Sciences)2010，53，7： | 12 |
| 8 | Ag nanoparticles sensitize IR-induced killing of cancer cells显示文摘 | Ruizhi Xu Jun Ma Xinchen Sun ZhongpingChen Xiaoli Jiang Zhirui auo Lan Huang YangLi Meng Wang Changling Wang Jiwei Liu XuFan Jiayu Gu Xi Chen Yu Zhang Ning Gu | 2009 | Cell Research2009，19，8： | 11 |
| 9 | Proportion of Uterine Malignant Tumors in Patients with Laparoscopic Myomectomy: A National Multicenter Study in China显示文摘 | Hua Yang Xiao-Chuan Li Chen Yao Jing-He Lang Hang-Mei Jin Ming-Rong Xi Gang Wang Lu-Wen Wang Min Hao Yan Ding Jie Chen Jian-Qing Zhang Lu Han Cheng-Xiu Guo Xiang Xue Yan Li Jian-Hua Zheng Man-Hua Cui Huai-Fang Li Guang-Shi Tao Long Chen Su-Min Wang An-Wei LU Ze-Hua Huang Qing Liu Ya-Li Zhuang Xiang-Hua Huang Gen-Hai Zhu Ou-Ping Huang Li-Na Hu Mu-Jun Li Hong-Lin Zhou Jing-Hui Song Lan Zhu | 2017 | Chinese Medical Journal2017，，22： | 10 |
| 10 | Preoperative albumin levels predict prolonged postoperative ileus in gastrointestinal surgery显示文摘BACKGROUND Prolonged postoperative ileus(PPOI) is a prolonged state of 'pathological'gastrointestinal(GI) tract dysmotility. There are relatively few studies examining the influence of preoperative nutritional status on the development of PPOI in patients who underwent GI surgery. The association between preoperative albumin and PPOI has not been fully studied. We hypothesized that preoperative albumin may be an independent indicator of PPOI.AIM To analyze the role of preoperative albumin in predicting PPOI and to establish a nomogram for clinical risk evaluation.METHODS Patients were drawn from a prospective hospital registry database of GI surgery.A total of 311 patients diagnosed with gastric or colorectal cancer between June 2016 and March 2017 were included. Potential predictors of PPOI were analyzed by univariate and multivariable logistic regression analyses, and a nomogram for quantifying the presence of PPOI was developed and internally validated.RESULTS The overall PPOI rate was 21.54%. Advanced tumor stage and postoperative opioid analgesic administration were associated with PPOI. Preoperative albumin was an independent predictor of PPOI, and an optimal cutoff value of 39.15 was statistically calculated. After adjusting multiple variables, per unit or per SD increase in albumin resulted in a significant decrease in the incidence of PPOI of 8%(OR = 0.92, 95%CI: 0.85-1.00, P = 0.046) or 27%(OR = 0.73, 95%CI:0.54-0.99, P = 0.046), respectively. Patients with a high level of preoperative albumin(≥ 39.15) tended to experience PPOI compared to those with low levels(< 39.15)(OR = 0.43, 95%CI: 0.24-0.78, P = 0.006). A nomogram for predicting PPOI was developed [area under the curve(AUC) = 0.741] and internally validated by bootstrap resampling(AUC = 0.725, 95%CI: 0.663-0.799).CONCLUSION Preoperative albumin is an independent predictive factor of PPOI in patients who underwent GI surgery. The nomogram provided a model to screen for early indications in the clinical setting. | Wen-Quan Liang Ke-Cheng Zhang Hua Li Jian-Xin Cui Hong-Qing Xi Ji-Yang Li Ai-Zhen Cai Yu-Hua Liu Wang Zhang Lan Zhang Bo Wei Lin Chen | 2020 | World Journal of Gastroenterology2020，26，11： | 10 |
| 11 | Epidemiology,clinical and laboratory characteristics of currently alive HIV-1 infected former blood donors naive to antiretroviral therapy in Anhui Province,China显示文摘Background Unregulated commercial blood/plasma collection among farmers occurred between 1992 and 1995 in central China and caused the second major epidemic of human immunodeficiency virus type 1 (HIV-1) infection in China. It is important to characterize HIV-1-infected former blood donors and to study characteristics associated with disease progression for future clinical intervention and vaccine development. Methods A cross-sectional study was performed on HIV-1-infected former blood donors (FBDs) and age-matched HIV-seronegative local residents. Demographic, epidemiologic, clinical and key laboratory data were collected from all study participants. Both unadjusted and adjusted multivariate linear regressions were employed to analyze the association of the decrease of CD4+ T-cell counts with other characteristics. Results Two hundred and ninety-four HIV-1-infected FBDs and 59 age-matched HIV-seronegative local residents were enrolled in this study. The unregulated blood/plasma collection occurred more than a decade (10.8 -12.8 years) ago, which caused the rapid spread of HIV-1 infection and the high prevalence of co-infection with hepatitis C virus (HCV, 89.5%); hepatitis B virus (HBV) co-infection was observed in only 11 HIV+participants (3.7%). Deterioration in both clinical manifestation and laboratory parameters and increase of viral loads were observed in parallel with the decrease of CD4+ T-cell counts. The decrease of total lymphocyte counts (P<0.001) and hemoglobin levels (P<0.001) and the appearance of dermatosis (P=0.03) were observed in parallel with the decrease of CD4+ T-cell counts whereas viral loads (P<0.001) and CD8+ T-cell counts (P=0.01) were inverselyassociated with CD4+ T-cell counts. Conclusions Co-infection with HCV but not HBV is highly prevalent among HIV-1-infected FBDs. CD4+ T-cell counts is a reliable indicator for disease progression among FBDs. Total lymphocyte counts, hemoglobin level and appearance of dermatosis were positively associated with CD8+ T-cell counts and viral loads were inversely associated with the decreased CD4+ T-cell counts. | XU Jian-qing WANG Jian-jun HAN Li-feng XU Chen RUAN Yu-hua XU Zhen-hou CHEN Xi LIU Zhen-dong WANG Jun SU Bing DING Xin-ping GAO Bing GU Yong-bin CAO Xiao-yun XING Hui HONG Kun-xue PENG Hong ZHAO Quan-bi YUAN Lin FENG Yi ZHANG Gui-yun MA Li-ying WU Lan SHAO Yi-ming | 2006 | Chinese Medical Journal2006，，23： | 9 |
| 12 | Fenton Reagent Generated in Electrolysis Cell and Its Usage in Degradation and Decolorization of Dyes显示文摘The Electro-chemistry process to produce Fenton reagents has been described. The in situ oxidation of dyes, acid chrome and alizarin red(Fenton reagents) with electrogenerated hydroxyl radicals was investigated. The \{ I-V \} cyclic voltammograms were measured. The redox peaks of the dyes were not observed in the treated dye solutions instead of a couple of O2/H2O2 redox peaks. The IR results indicate that acid chrome dye was decomposed into naphthylamine and phenol aminophenol sulfonic acid. The degradation and the decolorization of the dyes were comfirmed by the visible spectrum and the chemical analysis. The COD cr removing rate was close to 80%. | CHEN Zhen ** , ZHENG Xi, CHEN Ri-yao, LAN Rui-fang, LIN Zhi-hong and CHEN Yu-feng Experiment Center, Fujian Normal University, Fuzhou 350007, P. R. ChinaZHANG Yi-kangLinyi Normal College, Linyi 276001, P. R. China | 2002 | Chemical Research in Chinese Universities2002，18，1： | 8 |
| 13 | A new tripodal rhodamine B derivative as a highly selective and sensitive fluorescence chemosensor for copper(Ⅱ)显示文摘A new tripodal rhodamine B derivative 2 was designed and synthesized by tripodal trialdehyde and rhodamine B hydrazide for the first time. This derivative could be used as a fluorescent chemosensor for the selective and sensitive determination of copper(II) in Tris-HCl buffer and ethanol aqueous mixed media. Under the optimum conditions described herein,fluorescence enhancement at 557/577 nm was linearly related to the concentration of copper(II) in the range of 0.10 to 10.00×10-5 mol·L-1,with a corre-lation coefficient of R2=0.9964 (n=15) and a detection limit of 1.129×10-7 mol·L-1 (the relative standard deviation for five repeated measurements at 4.00×10-5 mol·L-1 Cu(II) was 2.2%). The absorbance meas-urements at 557 nm were linearly related to the concentration of Cu(II) in the range of 0.50 to 25.00×10-5 mol·L-1,with a correlation coefficient of R2=0.9948 (n=13) and a detection limit of 3.338×10-7mol·L-1. | ZENG Xi,WU Chong,DONG Lei,MU Lan,XUE SaiFeng & TAO Zhu College of Chemistry and Chemical Engineering ,Guizhou University,Guiyang 550003,China Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province,Guizhou University,Guiyang 550025,China | 2009 | Science China Chemistry2009，52，4： | 7 |
| 14 | A specific objective supplemental factor in evaluating acute mountain sickness: ΔHR in combination with SaO_2显示文摘Background: So far, there have been no measurements confirmed useful in diagnosing acute mountain sickness(AMS). The aim of this study was to determine the role of heart rate(HR) difference(ΔHR) and oxygen saturation(Sa O2) as objective risk factors in aiding the diagnosis of AMS.Methods: A total of 1,019 participants were assigned to either the acute exposure group(AEG): from 500 m to 3,700 m by flight within 2.5 hours(n=752); or the pre-acclimatization group(PAG): ascended to 4,400 m from 3,650 m within three hours by car after adapting 33 days at 3,650m(n=267). The questionnaires or measurements of resting Sa O2 and HR were completed between 18 and 24 hours before departure and after arrival.Results: Incidence of AMS was 61.3%(461) in AEG, with 46.1%(347) mild cases and 15.2%(114) severe cases. In PAG, the incidence was 38.9%(104), with 30.7%(82) mild cases and 8.2%(22) severe cases. The AMS subjects showed a significant increase in HR and a decrease in Sa O2 levels compared with the non-AMS subjects in both groups. ΔHR and post-exposure Sa O2 were significantly correlated with the Lake Louise Score(LLS) in both groups. Stepwise logistic regression analysis revealed the ΔHR >25 and Sa O2 <88% in AEG as well as ΔHR >15 and Sa O2 <86% in PAG to be independent risk factors of AMS. Combining these two measurements could specifically indicate participants with AMS, which showed a positive predictive value of 89% and specificity of 97% in AEG as well as 85% and 98% in PAG.Conclusion:ΔHR or Sa O2, as objective measurements, correlate with AMS. Combination of these two measurements may be useful as an additional specific and objective factor to further confirm the diagnosis of AMS. | Ming Li Ji-Hang Zhang Guo-Xi Zhao Shi-Zhu Bian Xu-Bin Gao Xi Liu Jie Yu Jun-Qing Dong Guo-Zhu Chen Hong Wang Lan Huang | 2015 | Military Medical Research2015，3，3： | 6 |
| 15 | Characterization of Class 1 Integron Gene Cassettes among Clinical Bacteria Isolated from One Large Hospital in Northern China显示文摘班在不同种类之中的 1 integron 和复杂基因盒子临床在北中国孤立在这研究被描绘。383 临床孤立从北中国,和包含广泛地在克之中散布的基因盒子的班 1 integrons 被获得否定临床孤立被观察。我们发现 1 integron 显示出的班与克的 multidrug 抵抗显型的积极关联否定细菌。另外,我们发现那孤立属于一种怀有基因盒子数组的不同类型,当基因盒子数组的一样的类型在不同种类被观察时孤立。在 isolates 之中的基因盒子数组的差异显示了 multidrug 抵抗的复杂性在临床在北中国孤立。 | CHEN Xia LI Gui Xi ZHANG Hong YUAN Min HOU Xiao Ping YU Hui Lan LI Juan | 2013 | Biomedical and Environmental Sciences2013，26，12： | 5 |
| 16 | Clinical effects of autologous stem cell transplantation as consolidation treatment in 70 multiple myeloma patients: a case-controlled study显示文摘背景自体同源的干细胞移植(ASCT ) 是多重骨髓瘤(公里) 的标准正式就职治疗的部分。这控制盒子的临床的试用试图进一步为公里作为巩固治疗评估 ASCT 的治疗学的效果并且讨论影响 70 个病人的临床的数据作为公里诊断了的 prognosis.Methods 的因素在我们在 1998 年 10 月和 2010 年 8 月之间的医院里作为巩固治疗收到了 ASCT 的人,回顾地被分析(ASCT 组) 。没有 ASCT (non-ASCT 组) ,在一样的时期期间收到平淡的化疗的另外的 70 个公里病人被用作控制。在宽恕,没有前进的幸存(PFS ) 和全面幸存(OS ) 的度和持续时间的差别与相比探索可以影响中部的后续时期是的 prognosis.Results 的因素 38 个月(范围 1-128 月) 。在在 ASCT 以后的到 51.4 的 ASCT (36/70 ) 前的组从 27.1 增加了的 ASCT 的完全的反应(CR ) 率(19/70 ) 。ASCT 组的中部的 PFS 比 non-ASCT 组显著地高(45 个月对 25 个月, P 0.001 ) 。ASCT 组的中部的 OS 也显著地更高(55 个月对 30 个月, P=0.016 ) 。单个因素的分析证明国际阶段系统(ISS ) 舞台,很好的部分反应(VGPR ) 或更好的结果显著地与 PFS 和 OS (P 0.001 ) 被相关。多因素分析证明 VGPR 或更好的结果是否被完成,是影响用作巩固治疗的疾病 prognosis.Conclusion 的独立因素, ASCT 能完成公里病人的更好的回答和更高的 OS 和 PFS。 | ZHOU Li-li YUAN Zhen-gang FU Wei-jun XI Hao ZHANG Chun-yang LAN Hai-feng ZHANG Xin CHEN Yu LIU Wen-yu HOU Jian | 2011 | Chinese Medical Journal2011，，19： | 4 |
| 17 | Prokaryotic Expression and Potential Application of the Truncated PCV-2 Capsid Protein显示文摘Three pairs of specific primers were designed to amplify the F2-1, F2-2 and XF2-2 truncated sequences of ORF2 which encodes the capsid protein of porcine circovirus type 2 (PCV-2). The F2-1 sequence had most of the NLS region of ORF2, but the F2-2 and XF2-2 genes had the NLS region deleted. Truncated genes were subcloned into pET-32a(+) vectors to construct recombinant fusion expression vectors. The vectors were then transformed into Rosetta(DE3) E. coli and expressed by induction of IPTG. Expressed proteins were detected by western blotting and ELISA. The protein with best immunoreactivity was confirmed and selected, then utilized to inoculate SPF rabbits to prepare polyclonal antibodies. The protein and prepared polyclonal antibody were utilized to detect sera samples against PCV-2 from Shandong province and PCV-2 particles in PK-15 cells. In our study, three recombinant fusion proteins were successfully obtained, and the molecular weights of fusion proteins were 35.9 kDa, 33.6 kDa and 38.6 kDa respectively detected by SDS-PAGE. All of the proteins showed positive reaction with anti-PCV-2 antisera, and His-XF2-2 showed better immunoreactivity than the others. The protein of His-XF2-2 was coated as antigen in ELISA to detect the seroprevalence of PCV-2 in certain districts of Shandong province, the seropositivity rate was 27.7 % (73/264). Specific fluorescence and positive signals for PCV-2 could be detected in PK-15 cells inoculated with PCV-2 with the participation of prepared antibodies against His-XF2-2 in IFA and IPMA. Experimental results indicated that the truncated PCV-2 ORF2 gene containing most of the NLS region was successfully expressed in E. coli, and His-XF2-2 was demonstrated to have better immunoreactivity with anti-PCV-2 antisera than the other two fusion proteins. His-XF2-2 and prepared polyclonal antibodies against it had a satisfactory capability in detecting PCV-2 infection. | Zhong-zi LOU Zhi-yong LI Gang WANG Jian-qiang LI Xi LAN Xue-rui LI Xiang-ping YIN Ji-xing LIU Si-dang LIU | 2010 | Virologica Sinica2010，25，2： | 4 |
| 18 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 19 | Cloning the Structure Genes and Expression the N Gene of Porcine Epidemic Diarrhea Virus DX显示文摘The structure genes spike(S) ,nucleocapsid(N) ,membrane(M) ,small membrane(sM) of a porcine epidemic diarrhea virus(PEDV) strain DX isolated in Gansu province,North-west of China,were cloned,sequenced and compared with published sequences of PEDV strains. The nucleotide sequences encoding the entire S,sM,M and N genes open reading frame(ORF) of DX were 4 152,231,681 and 1 326 bases long respectively. There were transcription regulatory sequences(TRSs) upstream of the initiator ATG of the S,N and M genes. The amino acids sequences of S,M and N contained 30,3 and 7 potential asparagine(N) -linked glycosylation sites. Homologous analysis and phylogenetic trees showed that DX had the closest relationship with strains LJB/06,JS-2004-2Z and CH/HLJH/06 that were also isolated from China and indicated the prevalence of some PEDV isolates in China were widespread since the JS-2004-2Z strain originated from the south of the China,and LJB/06 and CH/HLJH/06 were isolated from northeast China. The N gene was cloned using two primers which contained Nco I and BamH I restriction enzyme sites and subcloned into expression vector pET30a. The recombinant plasmid was then transformed into E.coli Rossta. SDS-PAGE showed there was a protein of about 55kDa as expected and Western blot indicated the N protein had biological activity. | Jian-qiang LI Ji-xing LIU Xi LAN Jie CHENG Run WU Zhong-Zi LOU Xiang-ping YIN Xue-rui LI Bao-yu LI Bin YANG Zhi-yong LI | 2009 | Virologica Sinica2009，24，3： | 4 |
| 20 | PHYSICAL MODELLING OF ISOTHERMAL DIE FORGING PROCESS OF Ti-ALLOY STRUCTURAL AIR-FRAME PART WITH ETYPE CROSS-SECTION AND VARYING THICKNESS RIB显示文摘Isothermal flashless die forging process of Ti - alloy structural air - frame part with varying thickness rib has been modelled in this paper.The results of present study show that a upside - down trapezoid rib would be formed and buckling would occure as blank is reduced,if the thickness of billet is maller than or equal to the thickness of rib. During modelling process of structural air frame part with E type cross - section rib, the saddle or lap would be formed finally at the middle of transverse rib between ribs with increase in deformation.If metal is allowed to flow out at confluence of longitudinal and transverse rib, the lop defect would be eliminated,, but a pipe cavity is obvious on corresponding loca- tion of blank. of defect formation depends on distance of metal flow ,friction,temperature homoge- neity of the blank and complexity of the part. | Z. K. Yao H. Z. Cuo M. Wang F. Lan and P. F. Feng (Northwestern Polytechnical University, Xi’ an 710072, China) | 2000 | Acta Metallurgica Sinica(English Letters)2000，13，1： | 4 |
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