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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | 基于现行高血压指南探究健康人群血清尿酸升高与高血压发病的相关性显示文摘本研究根据欧洲心脏病协会(European Society of Cardiology,ESC)和美国心脏病学会(American College of Cardiology,ACC)指南,探讨血清尿酸升高与高血压发病的相关性。方法和结果:本研究为回顾性研究,研究人员纳入年龄≥30岁、2016年接受常规年度体检并记录诊室血压数据的健康个体10 537人,其中7 349人2017年再次进行检查。高血压定义为诊室血压≥140/90(ESC指南)或130/80 mm Hg(1mm Hg=0.133kPa)(ACC指南)。 | 袁源(摘译) 郑武洪(审校) Liu CW Ke SR Tseng GS Wu YW Hwang JJ | 2021 | 中华高血压杂志2021，29，8： | 3 |
| 3 | Virtual histology intravascularultrasound analysis of non-culprit attenuated plaques detectedbygrayscale intravascular ultrasound in patients with acute coronarysyndromes显示文摘 | Wu X Maehara A Mintz GS | 2010 | Am J Cardiol2010，105，1： | 1 |
| 4 | Rates of cutaneous metastases from different internal malignancies : experience from a Taiwanese medical center 显示文摘 | Hu SC Chen GS Wu CS | 2009 | J Am Acad Dermatol2009，60，3： | 1 |
| 5 | KILLER/DR5 is a DNA damage-inducible p53regulated death receptor gene显示文摘 | Wu GS Burns TF McDonald ER | 1997 | Nat Genet1997，17，： | 1 |
| 6 | Chronic treatment with qiliqiangxin amel- iorates aortic endothelial cell dysfunction in diabetic rats显示文摘 | Chen F Wu JL Fu GS | 2015 | J Cardio-vase Pharmacol Ther2015，20，2： | 1 |
| 7 | Role of autophagy in Cisplatin resistance in ovarian cancer cells 显示文摘 | Wang J Wu GS | 2014 | Journal of Biological Chemistry2014，289，24： | 1 |
| 8 | Combined MMF and insulin therapy prevents renal injury in experimental diabetic rats显示文摘 | Wu XY Zha DQ Xiang GS | 2006 | Cytokine2006，36，56： | 1 |
| 9 | Eight new 2-(2-phenylethyl) chromone (=2- (2-Phenylethyl)-4H-l-benzopyran-4-one )derivatives from Aquilaria malaccensis agarwood显示文摘 | Wu B Kwon SW Hwang GS | 2012 | Helv Chim Acta2012，95，： | 1 |
| 10 | Potential role for cathepsin D in p53-dependent tumor suppression and chemosensitivity 显示文摘 | Wu GS Saftig P Peters C | 1998 | Oncogene1998，16，17： | 1 |
| 11 | Peroxynitrite-induced apoptosis in photoreceptor cells显示文摘 | Ito S Wu GS Kimoto T | 2004 | Curr Eye Res2004，28，1： | 1 |
| 12 | An analysis of anesthesia-controlled opera- ting room time after propofol-based total intravenous anesthesia compared with desflurane anesthesia in ophthalmic surgery :a retrospective study显示文摘 | Wu ZF Jian GS Lee MS | 2014 | Anesth Analg2014，119，6： | 1 |
| 13 | Oct-3/4 expression re- flects tumor progression and regulates motility oar b/adder cancer cells显示文摘 | Chang CC Shieh GS Wu P | 2008 | Cancer Res2008，68，15： | 1 |
| 14 | Peritumoral edema on magnetic resonance imaging predicts a poor clinical outcome in malignant glioma显示文摘 | Wu CX Lin GS Lin ZX | 2015 | Oncol Lett2015，10，5： | 1 |
| 15 | The Saceharomyees cerevisiae Lipin homolog is a Mg2+-dependent phosphatidate phosphatase enzyme显示文摘 | Han GS Wu WI Carman GM | 2006 | J Biol Chem2006，281，： | 1 |
| 16 | TRAIL as a target in anti-cancer therapy显示文摘 | Wu GS | | 0，，1： | 1 |
| 17 | Oct3/4 Expression reflects tumor progression and regulates motility of bladder cancer cells 显示文摘 | Chang CC Shieh GS Wu P | 2008 | Cancer Res2008，68，15： | 1 |
| 18 | Development and evaluation of functional gene arrays for detection of selected genes in the environment 显示文摘 | Wu LY Thompson DK Li GS | 2001 | Appl Environ Microbial2001，67，12： | 1 |
| 19 | 显示文摘 | Tang ZH Qiu WH Wu GS | 2002 | World J Gastroenterol2002，8，5： | 1 |
| 20 | Multiple risk factors associated with arsenic-induced skin cancer: effects of chronic liver disease and malnutritional status 显示文摘 | Hsueh YM Cheng GS Wu MM | 2009 | Br J Cancer2009，71，1： | 1 |
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