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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | 塞来昔布治疗骨关节炎的随机对照试验显示文摘目的 :比较塞来昔布、萘普生以及安慰剂在治疗膝关节骨关节炎时的疗效、安全性。方法 :10 0 3名有症状的膝关节骨关节炎患者随机接受塞来昔布 5 0 ,10 0 ,2 0 0mg ,bid ;萘普生 5 0 0mg ,bid或安慰剂 12周。患者在停用既往NSAID药或止痛治疗后 2~ 7d以及服用受试药物治疗后 2 ,6 ,12周 ,根据标准疗效判定方法进行评估。结果 :塞来昔布在改善骨关节炎的症状和体征上有显著效果。在治疗后的 2d内疼痛明显缓解 ,最大抗炎和镇痛作用出现在 2周内并持续至 12周。各剂量的塞来昔布与安慰剂比较均有效 ,但 5 0mg ,bid的剂量效果较差 ;较大剂量的塞来昔布 (10 0和 2 0 0mg ,bid)疗效相似 ,所观察到的改善程度与萘普生 5 0 0mg ,bid相当。各剂量的塞来昔布和萘普生均有良好的耐受性。结论 :COX 2抑制剂塞来昔布是治疗骨关节炎的有效药物 ,对骨关节炎临床症状和体征的改善与萘普生相当。 | Bensen WG Fiechtner JJ Mcmillen JI Zhao WW Yu SS Woods EM Hubbard RC Isakson PC Verburg KM Geis GS | 2000 | 中国新药杂志2000，9，8： | 17 |
| 3 | Protective effect of electroacupuncture and moxibustion on gastric mucosal damage and its relation with nitric oxide in rats显示文摘INTRODUCTION Gastric mucosal injury is one of the common disorders,there are many reports subjicted to its pathogenesis treatment and prevention.We investigated the protective effect | Pei WF Xu GS Sun Y Zhu SL Zhang DQ | 2000 | World Journal of Gastroenterology2000，6，3： | 8 |
| 4 | Microstructure of Al-Si Alloys Rapidly Solidified from the Different Temperature Melts显示文摘Hypo- and hyper-eutectic Al-Si alloys were rapidly solidified from the low or high temperature melt using water quenching and melt-spinning technique. The differences in microstructure of rapid solidification alloys were studied by scanning and transmission electron microscopy,and X-ray diffraction methods. The primary silicon in the Al-22(wt pct)Si alloy quenched in room temperature water from high temperature melt was smaller than that quenched from low temperature melt. Compared with the ribbons spun from low temperature melt, the ribbons spun from high temperature melt had finer Al-rich and Si-rich phase particles in either Al-7(wt pct)Si or Al-18(Wt pct)Si alloy. The mechanism was explained by the nature of the liquid | Gui, MC Jia, J Song, GS Li, QC | 1999 | Journal of Materials Science & Technology1999，15，3： | 7 |
| 5 | 螺内酯与可乐定作为难治性高血压第4种治疗药物的比较:难治性高血压的最佳治疗随机研究显示文摘该研究旨在通过一个多中心随机试验对作为难治性高血压患者的第4种治疗药物的螺内酯与可乐定进行比较。采用药丸计数评估药物治疗依从性。难治性高血压患者(定义为包括1种利尿剂在内的3种药物治疗12周,诊室及动态血压监测血压仍未控制)随机给予螺内酯(12.5~50 mg/d)或可乐定(0.1~0.3mg,2次/d)继续治疗12周。 | Krieger EM Drager LF Giorgi DMA Pereira AC Barreto-Filho JAS Nogueira AR Mill JG Lotufo PA Amodeo C Batista MC Bodanese LC Carvalho ACC Castro I Chaves H Costa EAS Feitosa GS Franco RJS Fuchs FD Guimarāes AC Jardim PC Machado CA Magalhāes ME Mion D Jr Nascimento RM Nobre F Nóbrega AC Ribeiro ALP Rodrigues-Sobrinho CR Sanjuliani AF Teixeira MDCB Krieger JE ReHOT Investigators 赵狄 练桂丽 | 2018 | 中华高血压杂志2018，26，3： | 7 |
| 6 | 儿童期肥胖、成年期肥胖和心血管风险因子显示文摘过去30年,儿童超重或肥胖的发生率明显增加[1]。儿童期肥胖是死亡率增加的预测因子,这主要是由于儿童期肥胖可导致心血管疾病发生率增加。相关的预测研究提示"肥胖流行症"可能改变目前心血管疾病死亡率下降的趋势,使现今儿童的寿命缩短。 | Juonala M Magnussen CG Berenson GS Venn A Burns TL Sabin MA Srinivasan SR Daniels SR Davis PH Chen W Sun C Cheung M Viikari JS Dwyer T Raitakari OT 叶鹏 | 2012 | 中华高血压杂志2012，20，1： | 6 |
| 7 | Recognition Interactions of Metal-complexing Imprinted Polymer显示文摘Molecularly imprinted polymer, exhibiting considerable enantioselectivity for L-mandelic acid, was prepared using metal coordination-chelation interaction. By evaluating the recognition characteristics in the chromatographic mode, the recognition interactions were proposed: specific and nonspecific metal coordination-chelation interaction and hydrophobic interaction were responsible for substrate binding on metal-complexing imprinted polymer; while the selective recognition only came from specific metal coordination-chelation interaction and specific hydrophobic interaction. | Liu, Y Ding, GS De Wang, J | 2005 | Chinese Chemical Letters2005，16，6： | 5 |
| 8 | 石杉碱甲对重度抑郁症患者认知功能障碍的治疗:一项随机对照试验的系统综述(英文)显示文摘背景:乙酰胆碱酯酶(Acetylcholinesterase,AChE)抑制剂在重性抑郁障碍(Major Depressive Disorder,MDD)的动物模型和人类患者中已被证实可以有效地治疗认知障碍。石杉碱甲(Huperzine A,HupA)是一种来自于被称为蛇足石杉(Huperzineserrata)的石松属传统中医药,是一种强有力的AChE抑制剂,已被用于抑郁症的辅助治疗,但有尚无关石杉碱甲对MDD的强化治疗作用的meta分析。目标:对有关石杉碱甲强化治疗抑郁症的随机对照试验进行系统综述和meta分析,评估其疗效及安全性。方法:两位评估者独立检索9个英文和中文数据库,选择符合预先确定的纳入标准的相关研究,提取有关疗效和安全性的数据,并进行质量评估和数据拟合合成。结果:纳入了三项中国低质量的随机对照试验(总共n=238),这些试验比较了单用抗抑郁药治疗抑郁症与抗抑郁药和石杉碱甲的联合治疗,试验中的被试从16岁到60岁。研究中石杉碱甲辅助抗抑郁药治疗的平均时间仅为6.7周。这三项研究都是公开标签未使用盲法,所以他们的总体质量评定为差。总体样本的Meta分析发现两组抑郁症状的改善没有显著性差异(差异加权差为-1.90,95%CI可信区间为-4.23至0.44,p=0.11)。然而,石杉碱甲辅助治疗组比单用抗抑郁药治疗组在认知功能和生活质量方面有显著改善(如威斯康星卡片分类测验、韦氏记忆量表修订的评估)。组间药物不良反应的发生率无显著性差异。结论:有关在接受抗抑郁药的MDD患者使用HupA辅助治疗的疗效和安全性的可获取数据不足,难以得出有关其疗效和安全性的明确结论。汇集国内3项低质量的RCT数据没有发现采用辅助使用HupA治疗抑郁症状的优势,但辅助使用HupA与更快改善经常伴随MDD出现的认知症状相关。 | 郑伟 向应强 Ungvari GS Chiu F.K.H Ng H.C 王颖 项玉涛 | 2016 | 上海精神医学2016，28，2： | 4 |
| 9 | 基于现行高血压指南探究健康人群血清尿酸升高与高血压发病的相关性显示文摘本研究根据欧洲心脏病协会(European Society of Cardiology,ESC)和美国心脏病学会(American College of Cardiology,ACC)指南,探讨血清尿酸升高与高血压发病的相关性。方法和结果:本研究为回顾性研究,研究人员纳入年龄≥30岁、2016年接受常规年度体检并记录诊室血压数据的健康个体10 537人,其中7 349人2017年再次进行检查。高血压定义为诊室血压≥140/90(ESC指南)或130/80 mm Hg(1mm Hg=0.133kPa)(ACC指南)。 | 袁源(摘译) 郑武洪(审校) Liu CW Ke SR Tseng GS Wu YW Hwang JJ | 2021 | 中华高血压杂志2021，29，8： | 3 |
| 10 | 脂质组学改善传统危险因素预测的2型糖尿病患者的心血管事件显示文摘临床脂质测定并未显示出糖尿病或心血管病相关的脂质代谢改变的复杂性。脂质组学则能够评估可作为疾病风险潜在标志物的数百种脂质种类。方法:纳入来自糖尿病治疗和血管保护行动。 | 刘莉 叶鹏 Alshehry ZH Mundra PA Barlow CK Mellett NA Wong G McConville MJ Simes J Tonkin AM Sullivan DR Barnes EH Nestel PJ Kingwell BA Marre M Neal B Poulter NR Rodgers A Williams B Zoungas S Hillis GS Chalmers J Woodward M Meikle PJ | 2016 | 中华高血压杂志2016，24，10： | 3 |
| 11 | 合成香料—叶醇显示文摘 | Clark,GS 李宏 | 1991 | 天津轻工1991，，1： | 3 |
| 12 | Early and late results of intracoronary arterial stenting after coronary angioplasty in dogs显示文摘 | Roubin GS Robinson KA King SB | 1987 | Circulation1987，76，4： | 2 |
| 13 | Radioiododeoxyu-eidine in cancer therapy: an in vitro approach to developing in vivo strategies显示文摘 | Schneiderman MH Schneiderman GS | 1996 | J Nucl Med1996，37，： | 2 |
| 14 | Restenosis after canary angioplasty显示文摘 | Hong MK Mehran R Mintz GS | 1997 | Current Problems in Cardiology1997，22，： | 2 |
| 15 | Overwhelming posts plenec tomy infection显示文摘 | Krivit W Giebink GS Leonard A | 1979 | Surg Clin North Am1979，59，2： | 2 |
| 16 | Inhibition of angiogenesis by recombinant human platelet factor- 4 and related peptides 显示文摘 | Maione TE Gray GS Petro J | 1990 | Science1990，247，： | 2 |
| 17 | Homocysteine and its role in the pathogenesis of atherosclerotic vascular disease显示文摘 | Sainani GS Sainani R | 2002 | J Assoc Physicians India2002，，： | 2 |
| 18 | Effects of VE on cadmiuminduced locomotor dysfunctions in rats显示文摘 | Ali MM Shukla GS Srivastava RS | 1993 | Vet Hum Toxicol1993，35，： | 2 |
| 19 | Association of gene and protein expression and genetic polymorphism of CC chemokine ligand 4 in colorectal cancer显示文摘BACKGROUND Leukocytes,such as T cells and macrophages,play an important role in tumorigenesis.CC chemokine ligand(CCL)4,which is produced by lymphocytes and macrophages,has been found to be expressed in the mucosa of the gastrointestinal tract and is a potent chemoattractant for various leukocytes.AIM To examine CCL4 expression and its genetic polymorphism rs10491121 in patients with colorectal cancer(CRC)and evaluate their prognostic significance.METHODS Luminex technology was used to determine CCL4 Levels in CRC tissue(n=98),compared with paired normal tissue,and in plasma from patients with CRC(n=103),compared with healthy controls(n=97).Included patients had undergone surgical resection for primary colorectal adenocarcinomas between 1996 and 2019 at the Department of Surgery,Ryhov County Hospital,Jönköping,Sweden.Reverse transcription quantitative PCR was used to investigate the CCL4 gene expression in CRC tissue(n=101).Paired normal tissue and TaqMan single nucleotide polymorphism assays were used for the CCL4 rs10491121 polymorphism in 610 CRC patients and 409 healthy controls.RESULTS The CCL4 protein and messenger RNA expression levels were higher in CRC tissue than in normal paired tissue(90%,P<0.001 and 45%,P<0.05,respectively).CRC tissue from patients with localized disease had 2.8-fold higher protein expression levels than that from patients with disseminated disease.Low CCL4 protein expression levels in CRC tissue were associated with a 30%lower cancer-specific survival rate in patients(P<0.01).The level of plasma CCL4 was 11%higher in CRC patients than in healthy controls(P<0.05)and was positively correlated(r=0.56,P<0.01)with the CCL4 protein level in CRC tissue.The analysis of CCL4 gene polymorphism rs10491121 showed a difference(P<0.05)between localized disease and disseminated disease in the right colon,with a dominance of allele A in localized disease.Moreover,the rate of the A allele was higher among CRC patients with mucinous cancer than among those with nonmucinous cancer.CONCLUSION The present study indicates that the CRC tissue levels of CCL4 and CCL4 gene polymorphism rs10491121,particularly in the right colon,are associated with clinical outcome in CRC patients. | Levar Shamoun Kalle Landerholm Amanda Balboa Ramilo Roland E Andersson Jan Dimberg Dick Wågsäter | 2021 | World Journal of Gastroenterology2021，27，30： | 2 |
| 20 | 符合HDMI 1.4a接口标准的3D电视测试信号显示文摘在影院中大放异彩的3D技术目前已可以在家庭中实现。最新的HDMI 1.4a标准为在消费电子产品中传输3D视频提供了依据。新的或更多先进的显示技术遵循该标准,就可以将视频数据转换为三维效果显示出来。然而,这些新技术也给3D电视制造商带来了新挑战。尤其在开发和质量认证环节。 | Harald Gsdl 吴敏 | 2011 | 电视技术2011，35，4： | 2 |
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