|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | 儿童期肥胖、成年期肥胖和心血管风险因子显示文摘过去30年,儿童超重或肥胖的发生率明显增加[1]。儿童期肥胖是死亡率增加的预测因子,这主要是由于儿童期肥胖可导致心血管疾病发生率增加。相关的预测研究提示"肥胖流行症"可能改变目前心血管疾病死亡率下降的趋势,使现今儿童的寿命缩短。 | Juonala M Magnussen CG Berenson GS Venn A Burns TL Sabin MA Srinivasan SR Daniels SR Davis PH Chen W Sun C Cheung M Viikari JS Dwyer T Raitakari OT 叶鹏 | 2012 | 中华高血压杂志2012，20，1： | 6 |
| 3 | Rates of cutaneous metastases from different internal malignancies : experience from a Taiwanese medical center 显示文摘 | Hu SC Chen GS Wu CS | 2009 | J Am Acad Dermatol2009，60，3： | 1 |
| 4 | Heat shock protein 70/MAGE-1 tumor vaccine can enhance the potency of MAGE-1-specific cellular immune responses in v ivo 显示文摘 | Ye J Chen GS Song HP | 2004 | Cancer Immunol Immunother2004，53，9： | 1 |
| 5 | Chronic treatment with qiliqiangxin amel- iorates aortic endothelial cell dysfunction in diabetic rats显示文摘 | Chen F Wu JL Fu GS | 2015 | J Cardio-vase Pharmacol Ther2015，20，2： | 1 |
| 6 | Circulating MicroRNAs in gyneco- logical malignancies: from detection to prediction显示文摘 | Zhao YN Chen GS Hong SJ | 2014 | Exp He- matol Oncol2014，3，： | 1 |
| 7 | Methylene blue attenuates acute liver injury induced by paraquat in rats显示文摘 | Chen JL Dai L Zhang P Chen W Cai GS Qi XW | 2015 | Int Immunopharmacol2015，28，1： | 1 |
| 8 | FK506 promotes melanocyte and melanoblast growth and creates a favourable milieu for cell migration via keratinocytes:possible mechanisms of how tacrolimus ointment induces repigmentation in patients with vitiligo显示文摘 | Lan CC Chen GS Chiou MH | 2005 | Br J Dermatol2005，153，3： | 1 |
| 9 | Reperfusion and neurovascular dysfunction in stroke: from basic mechanisms to potential strategies for neuroprotection 显示文摘 | Jung JE Kim GS Chen H | 2010 | Mol Neurobiol2010，41，23： | 1 |
| 10 | Differential expression of plasminogeu activator and their blhibitors in an organotypic skin cocuhure system 显示文摘 | Chen CS Lyons-Giordano B Lazarus GS et at | 1993 | J Cell Sci1993，106，1： | 1 |
| 11 | Valproate pretreatment protects dopaminergic neurons from LPS-induced neurotoxicity in rat primary midbrain cultures: role of microglia显示文摘 | Peng GS Li G Tzeng NS Chen PS Chuang DM Hsu YD Yang S Hong JS | 2006 | 中国生物学文摘2006，20，10： | 1 |
| 12 | FK506 promotesmelanocyte and melanoblast growth and creates a favourablemilieu for cell migration via keratinocytes:possible mechanismsof how tacrolimus ointment induces repigmentation in patientswith vitiligo显示文摘 | LAN CC CHEN GS CHIOU MH | 2005 | Br J Dermatol2005，153，3： | 1 |
| 13 | Nonablative 1 450-nm di- ode laser treatment for xanthoma disseminatum显示文摘 | Hsu MC Lee CH Chen GS | 2014 | Derma- tol Surg2014，40，12： | 1 |
| 14 | Enantioselective determination of chlorpheniramine in various formulations by HPLC using carboxymethyl-beta-cyclodextrin as a chiral addi- tive 显示文摘 | ]Chen QC Jeong S J Hwang GS | 2008 | Arch Pharm Res2008，31，4： | 1 |
| 15 | Shiga toxin - producing Escherichia coli in Montana: bacterial genotypes and clinical profiles 显示文摘 | Jelacie JK Damrow T Chen GS | 2003 | J Infect Dis2003，188，5： | 1 |
| 16 | Determination of extracellular glutathione in lives of anaesthetized rats by microdialysis with on-line high-perfo-rmance liquid chromatography 显示文摘 | Yang GS Tsai PJ Chen WY | 1995 | Journal of Chromatography B1995，667，： | 1 |
| 17 | Influence of lipoprotein lipase serine 447 stop polymorphism on tracking of triglycerides and HDL cholesterol from childhood to adulthood and familial risk of coronary artery disease: the Bogalusa Heart Study显示文摘 | Chen W Srinivasan SR Elkasabany A Ellsworth DL Boerwinkle E Berenson GS | 2001 | Atherosclerosis2001，159，2： | 1 |
| 18 | Isolated u-shaped sacral fracture with cauda equina injury显示文摘 | Chen HW Liu GD Zhao GS | 2011 | Orthopedics2011，34，4： | 1 |
| 19 | Generalized chorea due to delayed encephalopathy after acute carbon monox- ide intoxication 显示文摘 | Sung YF Chen MH Peng GS | 2015 | Ann Indian Acad Neurol2015，18，1： | 1 |
| 20 | Expression of livin and vascular endothe- lial growth factor in different clinical stages of human esophageal carci- noma显示文摘 | Chen L Ren GS Li F | | World J Gastroenterol0，14，37： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员