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| 1 | A new method for determination of antithrombotic activity of egg white protein hydrolysate by microplate reader显示文摘A new method for the determination of antithrombotic activity of egg white protein hydrolysate(EWPH)was developed using amicroplate reader.Reaction was carried out at 37℃ and pH 7.2 with fibrinogen concentration 0.1%.Microplate reading wasconducted at 405 nm.Inhibition rate of EWPH on thrombin activity showed linearity(R^2=0.9971),when the inhibition rate was inthe range of 10-90%.The lower limit of detection(LLD,at 99.7%probability)and the biological limit of detection(BLD,at 99.7%probability)of the method were 10.643 and 40 mg/mL,respectively.The repeatability standard deviation(R.S.D.)was 1.08%.Thestandard deviation of the method was 0.027 AT-U. | Wan Gen Yang Zhang Wang Shi Ying Xu | 2007 | Chinese Chemical Letters2007,18,4: | 15 |
| 2 | A New Alkaloid from the Root of Isatis indigotica Fort显示文摘A E-2-[(3’-indole)cyanomethylene]-3-indolinone was isolated from the root of Isatis indigotica Fort. The structure elucidation and 1H, 13C NMR assignments were achieved by spectroscopic method. | Wan Sheng CHEN*, Bin LI, Wei Dong ZHANG, Gen Jing YANG, Chuan Zhuo QIAO Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433 | 2001 | Chinese Chemical Letters2001,12,6: | 11 |
| 3 | Salidroside Reduces PDE2A Expression by Down-regulating p53 in Human Embryonic Lung Fibroblasts显示文摘Salidroside(SAL) is a glucoside of tyrosol and a key constituent of Rhodiola rosea L. The diverse pharmacological activities of SAL include anti-inflammatory, cardioprotective, and neuroprotective effects(1)Our previous investigation showed that the anti-aging effects of SAL involve reductions of the levels of advanced glycation end products in a D-galactose induced mouse model(2)Additionally. | XING Wen Min CHEN Sha Sha WANG San Ying GAO Wen Yan WAN Xiao Qing SU Hui Li YANG Yi ZHANG Jing YAN Jing MAO Gen Xiang | 2019 | Biomedical and Environmental Sciences2019,32,2: | 3 |
| 4 | Two New Glucosides from Radix Polygoni multiflori Preparata显示文摘2,3,5,4’-tetrahydroxystilbene-2-O-(2’’-O-feruloyl)-(-D-glucopyranoside, 2,3,5,4’-tetra hydroxystilbene-2-O-(2’’-O-p-coumaroyl)-(-D-glucopyranoside were isolated from Radix Polygoni multiflori Preparata. Structures were elucidated by chemical and spectral evidences. | Wan Sheng CHEN*, Wei Dong ZHANG, Gen Jing YANG, Hai Sheng CHEN, Chuan Zhuo QIAO Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433 | 2001 | Chinese Chemical Letters2001,12,6: | 3 |
| 5 | Synthesis of N-methylene phosphonic chitosan(NMPCS)and its potential as gene carder显示文摘N-Methylene phosphoulc chitosan (NMPCS),an amphiphilic macromolecule with powerful chelating ability of Ca^(2+) ions,was synthesized and characterized.The physicochemical properties of NMPCS and the interactions between NMPCS and plasmid DNA were investigated by FTIR,^(13)C NMR,X-ray,agarose gel electrophorcsis retardation assay,atomic force microscopy (AFM) and circular dichroism (CD).The results suggest that at charge ratio 2:1 or above,DNA could be completely entrapped and spherical complexes with mean size of 80-210 nm were formed.Taking HeLa as host cell,luciferase expression mediated by NMPCS improved about 100 times compared to the expression mediated by chitosan. | Dun Wan Zhu Jin Gen Bo Hai Ling Zhang Wen Guang Liu Xi Gang Leng Cun Xian Song Yu Ji Yin Li Ping Song Lan Xia Liu Lin Mei Xiu Lan Li Yang Zhang Kang De Yao | 2007 | Chinese Chemical Letters2007,18,11: | 3 |
| 6 | Microcrystallization and lattice contraction of NiFe LDHs for enhancing water electrocatalytic oxidation显示文摘The lattice-oxygen-mediated mechanism is considered as a reasonable mechanism for the electrochemical catalytic oxygen evolution reaction(OER)of NiFe layered double hydroxides(LDHs).A NiFe LDH with distinct lattice contraction and microcrystallization was synthesized via a simple one-step method using sodium gluconate.The lattice contraction is attributed to the interaction of carbon in sodium gluconate and iron in NiFe LDH.The NiFe LDH with optimized microcrystallization and lattice contraction shows a low overpotential of 217 mV at a current density of 10 mA cm^(−2) and excellent durability of 20 h at a high current density of 100 mA cm^(−2).The results revealed that a contractive metal–oxygen bond could boost the intrinsic activity of active sites and the microcrystallization promotes an increase in the number of active sites in terms of unit area.The chemical environment of oxygen elemental characterization and resistance at different chronopotentiometry times confirm that the lattice oxygen element is indeed involved in the process of OER,supporting the lattice-oxygen-mediated mechanism of NiFe LDH.Density functional theory calculations reveal that contractive metal–oxygen bonds induced a reduction of the adsorption energy barrier of intermediate products,thus improving the intrinsic catalytic activity.The special characteristics of microcrystallization and lattice contraction of NiFe LDH provide a strategy to improve both the number and the intrinsic activity of active sites in a versatile manner. | Zhicheng Zheng Dan Wu Gen Chen Ning Zhang Hao Wan Xiaohe Liu Renzhi Ma | 2022 | Carbon Energy2022,4,5: | 2 |
| 7 | The ε-Complexity of the Approximate Solution of Integral Equations with Isotropic Kernels | Wan MA Gen Sun FANG College of Mathematics & Information Science of Wenzhou University, Wenzhou 325027, P. R. China Department of Mathematics, Beijing Normal University, Beijing 100875, P. R. China | 2002 | Acta Mathematica Sinica,English Series2002,18,4: | 1 |
| 8 | Anchoring Active Sites by Pt_(2)FeNi Alloy Nanoparticles on NiFe Layered Double Hydroxides for Efficient Electrocatalytic Oxygen Evolution Reaction显示文摘Strategy of anchoring alloy nanoparticles made up of the efficient catalytic element(e.g.,Ni,Fe)on dodecyl sulfate(DS^(-))-intercalated NiFe layered double hydroxides(DS^(-)-NiFe LDH)obtained by a convenient one-step hydrothermal coprecipitation method for essentially enhancing oxygen evolution reaction(OER)performance was proposed.The results of structural characterization indicate Pt_(2)FeNi alloy nanoparticles evenly distribute on the surface of DS^(-)-NiFe LDH.The sizes of the Pt_(2)FeNi nanoparticles,closely related to their OER performance,could be wellcontrolled by adjusting the amount of H;PtCl;addition.The composite structure of as-prepared product was stable during processes of synthesis,exfoliation,self-assembly,and subsequent electrocatalytic OER.Rigorous electrochemical test proving the contributing catalytic active sites was located at the interface between Pt_(2)FeNi and DS^(-)-NiFe LDH,and the Ni and Fe were the major active elements while O atoms are adsorption sites.The formation of Pt_(2)FeNi nanoparticles could greatly prompt the reduction of Tafel slope.The best-performing Pt_(2)FeNi/DS^(-)-NiFe LDH with a Pt content of 0.98 wt%achieved low overpotential of 204 mV at 10 mA cm^(-2)and 262 mV at 50 mA cm^(-2).This work provides a convenient and effective strategy to create additional active sites for enhancing OER performance of NiFe LDH and make contribution to its wide application. | Zhicheng Zheng Yanru Guo Hao Wan Gen Chen Ning Zhang Wei Ma Xiaohe Liu Shuquan Liang Renzhi Ma | 2022 | Energy & Environmental Materials2022,5,1: | 1 |
| 9 | In Vivo Lipid Regulation Mechanism of Polygoni Multiflori Radix in High-Fat Diet Fed Rats显示文摘 | Pei Lin Yan Ran He Jian Mei Lu Na Li Wan Gen Wang Wen Gu Jie Yu Rong Hua Zhao Kashmira Nanji | 2014 | Evidence-Based Complementary and Alternative Medicine2014,,: | 1 |
| 10 | Exploring the Reactivity of Multicomponent Photocatalysts: Insight into the Complex Valence Band of BiOBr显示文摘 | Yan‐Fen Fang Wan‐Hong Ma Ying‐Ping Huang Gen‐Wei Cheng | 2013 | Chem Eur J2013,,9: | 1 |
| 11 | Nondentritic Equiaxed Zone Formation in Aluminum-Lithium Welds显示文摘 | Gutierrez Alexander Lippold John C. Lin Wan Gen | 1996 | Materials Science Forum1996,,217: | 1 |
| 12 | Salt-induced Electron Transfer Photooxidation of Cholesterol in Nonpolar Solvents显示文摘Salt┐inducedElectronTransferPhotooxidationofCholesterolinNonpolarSolventsYANGChen-gen*(DepartmentofOrganicChemistry,Agricultu... | YANG Chen gen * (Department of Organic Chemistry, Agricultural College, Yangzhou University, Yangzhou, 225009) WAN Yan and JIANG Zhi qin (Analysis and Research Center, Tongji University, Shanghai, 200092) | 1997 | Chemical Research in Chinese Universities1997,13,4: | 0 |
| 13 | A Chromosome-level Reference Genome of African Oil Palm Provides Insights into Its Divergence and Stress Adaptation显示文摘The palm family(Arecaceae),consisting of2600 species,is the third most economically important family of plants.The African oil palm(Elaeis guineensis)is one of the most important palms.However,the genome sequences of palms that are currently available are still limited and fragmented.Here,we report a high-quality chromosome-level reference genome of an oil palm,Dura,assembled by integrating long reads with150 genome coverage.The assembled genome was 1.7 Gb in size,covering 94.5%of the estimated genome,of which 91.6%was assigned into 16 pseudochromosomes and 73.7%was repetitive sequences.Relying on the conserved synteny with oil palm,the existing draft genome sequences of both date palm and coconut were further assembled into chromosomal level.Transposon burst,particularly long terminal repeat retrotransposons,following the last whole-genome duplication,likely explains the genome size variation across palms.Sequence analysis of the VIRESCENS gene in palms suggests that DNA variations in this gene are related to fruit colors.Recent duplications of highly tandemly repeated pathogenesis-related proteins from the same tandem arrays play an important role in defense responses to Ganoderma.Whole-genome resequencing of both ancestral African and introduced oil palms in Southeast Asia reveals that genes under putative selection are notably associated with stress responses,suggesting adaptation to stresses in the new habitat.The genomic resources and insights gained in this study could be exploited for accelerating genetic improvement and understanding the evolution of palms. | Le Wang May Lee Zi Yi Wan Bin Bai Baoqing Ye Yuzer Alfiko Rahmadsyah Rahmadsyah Sigit Purwantomo Zhuojun Song Antonius Suwanto Gen Hua Yue | 2023 | Genomics, Proteomics & Bioinformatics2023,21,3: | 0 |
| 14 | Homopolymerization of N-pyrimidinyl acrylamide显示文摘Homopolymerization of N-pyrimidinyl acrylamide(NPA)was reported for the first time.The polymer(polyNPA)was soluble only in acidic media and fluoroalcohols,and only in fluoroalcohol was homogeneous polymerization of NPA feasible.~1H NMR analysis proved that a 1:1 H-bonding complex could be formed between NPA andα,α-bis(trifluoromethyl)phenyl propan-2-ol (BTMP).Cumyl dithiobenzoate mediated reversible addition-fragmentation chain transfer(RAFT)polymerization of NPA in BTMP was carried out.~1H NMR analyses proved that the molecular weight increased linearly with the monomer conversion.The polymer prepared in conventional solvents was atactic while in protic media the syndiotacticity was slightly enhanced. | De Cheng Wan Hong Ting Pu Gen Jin Yang | 2007 | Chinese Chemical Letters2007,18,9: | 0 |
| 15 | In Vitro Antibacterial Effect of Effective Microorganisms( EM) on Aeromonas hydrophila显示文摘In order to understand in vitro antibacterial effect of antibiotics and EM on Aeromonas hydrophila,14 isolates of A. hydrophila were isolated from diseased fish and aquaculture in the study. PCR amplification results of aerolysin gene( Aero gene) of isolates indicated that 7 out of 14 isolates were pathogenic A.hydrophila. In vitro antibacterial test indicated that 14 isolates were resistance to penicillin G,ampicillin,cephradine,cefazolin,tetracycline,terimethoprim,lincomycin and cephalexin,but showed high sensitivity to cefotaxime and furazolidone. In vitro antibacterial effect of EM on 14 isolates of A. hydrophila was positively correlated with concentration,and EM with the concentration greater than 60% had antibacterial effect on 14 isolates. | Zhu Zhixiu He Houjun Zhang Wenbo Wu Xiangdong Wan Gen Deng Shunzhou Liu Songlin | 2014 | Animal Husbandry and Feed Science2014,6,2: | 0 |
| 16 | Synthesis and Thermal Stability of Antimony Tris (Thioethyl Stearate) for PVC显示文摘A novel type of thermal stabilizer antimony tris (thioethyl stearate)(ATS) was synthsized. Its thermal stability was measured by heat aging oven test when incorporated into PVC. ATS was synthesized from stearic acid, antimony trioxide and 2 mercaptoethanol in two steps. The reaction conditions of synthesis were optimized through orthogonal test. Experimental results show that the molar ratio of stearic acid and antimony tris (2 hydroxyethylthiolate) was 1.2, adding 0.6% tetra n butyl titanate as catalyst and xylene as azeotropic solvent, heating and refluxing for about 4 h, and the yield of ATS is 83.9%. The thermal stability time is about 40 min(at 200 ℃) when added 2phr(per hundred resin) in PVC. The thermal stability of ATS is better than that of Ca Zn complex and basic lead stabilizers, and equal to that of dibutyltin dilaurate. | LIU You nian,WANG Chun jiang,SHU Wan gen,ZENG Dong ming,Chen Qi yuan (College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China) | 2000 | Journal of Central South University2000,12,3: | 0 |