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| 1 | Prunus genetics and applications after de novo genome sequencing:achievements and prospects显示文摘Prior to the availability of whole-genome sequences,our understanding of the structural and functional aspects of Prunus tree genomes was limited mostly to molecular genetic mapping of important traits and development of EST resources.With public release of the peach genome and others that followed,significant advances in our knowledge of Prunus genomes and the genetic underpinnings of important traits ensued.In this review,we highlight key achievements in Prunus genetics and breeding driven by the availability of these whole-genome sequences.Within the structural and evolutionary contexts,we summarize:(1)the current status of Prunus whole-genome sequences;(2)preliminary and ongoing work on the sequence structure and diversity of the genomes;(3)the analyses of Prunus genome evolution driven by natural and man-made selection;and(4)provide insight into haploblocking genomes as a means to define genome-scale patterns of evolution that can be leveraged for trait selection in pedigree-based Prunus tree breeding programs worldwide.Functionally,we summarize recent and ongoing work that leverages whole-genome sequences to identify and characterize genes controlling 22 agronomically important Prunus traits.These include phenology,fruit quality,allergens,disease resistance,tree architecture,and self-incompatibility.Translationally,we explore the application of sequence-based marker-assisted breeding technologies and other sequence-guided biotechnological approaches for Prunus crop improvement.Finally,we present the current status of publically available Prunus genomics and genetics data housed mainly in the Genome Database for Rosaceae(GDR)and its updated functionalities for future bioinformatics-based Prunus genetics and genomics inquiry. | Maria JoséAranzana Véronique Decroocq Elisabeth Dirlewanger Iban Eduardo Zhong Shan Gao Ksenija Gasic Amy Iezzoni Sook Jung Cameron Peace Humberto Prieto Ryutaro Tao Ignazio Verde Albert G.Abbott Pere Arús | 2019 | Horticulture Research2019，6，1： | 8 |
| 2 | Molecular charac- terization of isoniazid-resist ant Mycobacterium tuberculosis clinical strainsi solated in the Philippines显示文摘 | HERRER AL V AL VERDE A SAIZP | 2004 | Inl J Antimicrob Agents2004，23，6： | 1 |
| 3 | Genetic linkage mapping in peach using morphological,RFLP and RAPD markers显示文摘 | RAJAPAKSE S BELTHOFF L E HE G ESTAGER A E SCORZA R VERDE I BALLARD R E BAIRD W V CALLAHAN A MONET R ABBOTT A G | 1995 | Theor Appl Genet1995，90，： | 1 |
| 4 | Assessing the value of an- other cycle in Gaussian process surrogate-based optimization显示文摘 | Queipo N V Verde A Pintos S | 2009 | Structural and Multidisciplinary Optimization2009，39，5： | 1 |
| 5 | Method for measuring antioxidant activity and its application to monitoring antioxidant capacity of wine显示文摘 | Fogliano V Verde V Randazzo G | 1999 | J Agric Food Chem1999，47，： | 1 |
| 6 | Gluten immunogenic peptide excretion detects dietary transgressions in treated celiac disease patients显示文摘BACKGROUND Life-long removal of gluten from the diet is currently the only way to manage celiac disease(CeD). Until now, no objective test has proven useful to objectively detect ingested gluten in clinical practice. Recently, tests that determine consumption of gluten by assessing excretion of gluten immunogenic peptides(GIP) in stool and urine have been developed. Their utility, in comparison with conventional dietary and analytical follow-up strategies, has not been fully established.AIM To assess the performance of enzyme-linked immunosorbent assay(ELISA) and point-of-care tests(PoCTs) for GIP excretion in CeD patients on gluten-free diet(GFD).METHODS We conducted an observational, prospective, cross-sectional study in patients following a GFD for at least two years. Using the Gastrointestinal Symptom Rating Scale questionnaire, patients were classified at enrollment as asymptomatic or symptomatic. Gluten consumption was assessed twice by 3-d dietary recall and GIP excretion(by ELISA in stool and PoCTs(commercial kits for stool and urine) in two consecutive samples. These samples and dietary reports were obtained 10 day apart one from the other. Patients were encouraged to follow their usual GFD during the study period.RESULTS Forty-four patients were enrolled, of which 19(43.2%) were symptomatic despite being on a GFD. Overall, 83 sets of stool and/or urine samples were collected.Eleven out of 44 patients(25.0%) had at least one positive GIP test. The occurrence of at least one positive test was 32% in asymptomatic patients compared with 15.8% in symptomatic patients. GIP was concordant with dietary reports in 65.9% of cases(Cohen′s kappa: 0.317). PoCT detected dietary indiscretions. Both ELISA and PoCT in stool were concordant(concomitantly positive or negative) in 67 out of 74(90.5%) samples. Excretion of GIP was detected in 7(8.4%) stool and/or urine samples from patients considered to be strictly compliant with the GFD by dietary reports.CONCLUSION GIP detects dietary transgressions in patients on long-term GFD, irrespective of the presence of symptoms. PoCT for GIP detection constitutes a simple homebased method for self-assessment of dietary indiscretions. | Ana Florencia Costa Emilia Sugai María de la Paz Temprano Sonia Isabel Niveloni Horacio Vázquez María Laura Moreno M.Remedios Domínguez-Flores Alba Mu?oz-Suano Edgardo Smecuol Juan Pablo Stefanolo Andrea F González Angel Cebolla-Ramirez Eduardo Mauri?o Elena F Verdú Julio César Bai | 2019 | World Journal of Gastroenterology2019，25，11： | 1 |
| 7 | Investigating the specificity of peptide adsorption on gold using molecular dynamics simulations 显示文摘 | VERDE A V ACRES J M MARAN AS J K | 2009 | Biomacromolecules2009，10，8： | 1 |
| 8 | Construction of a genetic linkage map and identification of molecular markers in peach rootstocks for response to peach tree short life syndrome显示文摘 | Blenda A V Verde L Georgi L L | | 0，，： | 1 |
| 9 | First year Wilkinson microwave anisotropy probe ( WMAP )observations: determination of cosmological parameters 显示文摘 | Spergel D N Verde L Peiris H V | 2003 | ApJS2003，148，： | 1 |
| 10 | Method for measuring antioxidant activity and itsapplication to monitoring the antioxidant capacity ofwines显示文摘 | FOGLIANO V VERDE V RANDAZZO G | 1999 | Agric Food Chem1999，47，3： | 1 |
| 11 | First Year Wilkinson Microwave Anisotropy Probe (WMAP) Observations : Determination of Cosmological Parameters显示文摘 | SPERGEL D N VERDE L PEIRIS H V | 2003 | Astrophys J Supp12003，148，1： | 1 |
| 12 | Influence of personal mastery on organizational performance through organizational learning and innovation in large firms and SMEs显示文摘 | Víctor J. García-Morales Francisco Javier Lloréns-Montes Antonio J. Verdú-Jover | 2007 | Technovation2007，，9： | 1 |
| 13 | Security in wireless Ad - Hoc networks - a survey 显示文摘 | Pietro R D Guarino S Verde N V | 2014 | Computer Communications2014，40，10： | 1 |
| 14 | Genetic linkagemapping in peach using morphological,RFLP and RAPD marker显示文摘 | Rajapakse S He G Estager A E Scorza R Verde B Baird W V Callahan A Monet R Abbott A G Belthoff L E | 1995 | Theoretical and Applied Genetics1995，90，34： | 1 |
| 15 | Construction of a genetic linkage map and identification of molecular markers in peach rootstocks for response to peach tree short life syndrome 显示文摘 | BIENDA A V VERDE L GEORGI L L | 2007 | Tree Genetica and Genomes2007，3，4： | 1 |
| 16 | The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity,domestication and genome evolution显示文摘 | The International Peach Genome Iniative Verde I Abbott A G Scalabrin S Jung S Shu S Marroni F Zhebentyayeva T Dettori M T Grimwood J Cattonaro F Zuccolo A Rossini L Jenkins J Vendramin E Meisel L A Decroocq V Sosinski B Prochnik S Mitros T Policriti A Cip | | 0，，： | 1 |
| 17 | First-year wilkinson microwave anisotropy probe ( WMAP )observations: implications for inflation显示文摘 | Peiris H V Komatsu E Verde L | 2003 | ApJS2003，148，： | 1 |
| 18 | First-year Wilkinson Microwave Anisotropy Probe (WMAP)observations: determination of cosmological parameters 显示文摘 | Spergel D N Verde L Peiris H V | 2003 | ApJS2003，148，： | 1 |
| 19 | First-year Wilkinson microwave anisotropy probe (WMAP) observations: Determination of cosmological parameters显示文摘 | SPERGEL D N VERDE L PEIRIS H V | 2003 | ApJ2003，148，： | 1 |
| 20 | Dispersion of the Gilbert-Elliott channel显示文摘 | Polyanskiy Y Poor H V Verdú S | | 0，，04： | 1 |
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