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| 1 | Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs显示文摘 | Qiwei Shan Yanpeng Wang Kunling Chen Zhen Liang Jun Li Yi Zhang Kang Zhang Jinxing Liu Daniel F. Voytas Xuelian Zheng Yong Zhang Caixia Gao | 2013 | Molecular Plant2013，6，4： | 37 |
| 2 | Robust Transcriptional Activation in Plants Using Multiplexed CRISPR-Act2.0 and mTALE-Act Systems显示文摘为内长的基因的柔韧的 transcriptional 激活的用户友好的工具高度在植物被要求。我们以前显示出那由撤销联系 CRISPR 的蛋白质组成的一个 dCas9-VP64 系统(dCas9 ) 9 与四辆双人脚踏车熔化了使活跃之物 VP16 (VP64 ) 能被用于的 transcriptional 的重复在植物的内长的基因的 transcriptional 激活。在这研究,我们在植物为提高的 transcriptional 激活开发了向量系统的第二代。我们为基于 dCas9 的 transcriptional 激活测试了多重策略,并且由 dCas9 和产出的修改指南 RNA 支架 gRNA2.0 (指明的 CRISPR-Act2.0 ) 发现那 VP64 的同时的招募更强壮的 transcriptional 激活比 dCas9-VP64 系统。而且,我们开发了复合抄写为在植物的多达四基因的同时的激活的像使活跃之物的受动器激活(mTALE 行为) 系统。我们的结果建议 mTALE 行为是比在测试的大多数情况中的 CRISPR-Act2.0 甚至更有效的。另外,我们用积极反馈环探索了织物特定的基因激活。有趣地,我们的学习比当由激活探针使不安时,其它可以引起目标基因 silencing 到 transcriptional 激活,和紧调整的基因的表明某些内长的基因是更顺从的。因此,这些新工具能被用来调查基因规章的网络和他们的控制机制。复合 CRISPR-Act2.0 和 mTALE 行为系统的汇编是两个基于优化并且克隆策略的 PCR 独立的金门和网关,它将在 dicots 和 monocots 便于 transcriptional 激活应用程序。 | Levi G. Lowder Jianping Zhou Yingxiao Zhang Aimee Malzahn Zhaohui Zhong Tzung-Fu Hsieh Daniel F. Voytas Yong Zhang Yiping Qi | 2018 | Molecular Plant2018，11，2： | 19 |
| 3 | ZFN,TALEN and CRISPR-Cas9 mediated homology directed gene insertion in Arabidopsis:A disconnect between somatic and germinal cells显示文摘Breakthroughs in the generation of programmable sequencespecific nucleases (SSNs),such as zinc finger nucleases (ZFNs), TAL effector nucleases (TALENs)and the RNA-directed nuclease CRISPR-associated protein 9 (Cas9),have greatly increased the ease of plant genome engineering (Voytas,2013;Malzahn et al., 2017).Programmable SSNs introduce a DNA double-strand break (DSB)at a target site in the genome that must be repaired by one of several endogenous DNA repair pathways. | Qiwei Shan Nicholas J.Baltes Paul Atkins Elida R. Kirkland Yong Zhang Joshua A.Ballet Levi G.Lowder Aimee A.Malzahn John C.Haugner Ⅲ Burckhard Seelig Daniel F. Voytas Yiping Qi | 2018 | Journal of Genetics and Genomics2018，45，12： | 9 |
| 4 | A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants显示文摘 | Tang, Xu Zheng, Xuelian Qi, Yiping Zhang, Dengwei Cheng, Yan Tang, Aiting Voytas, Daniel F. Zhang, Yong | 2016 | Molecular Plant2016，9，7： | 8 |
| 5 | Non-transgenic Plant Genome Editing Using Purified Sequence-Specific Nucleases显示文摘Dear Editor, Sequence-specific nucleases,including zinc-finger nucleases,meganucleases,TAL effector nucleases (TALENs),and CRISPR/ Cas systems,have been used to introduce targeted mutations in a wide range of plant species (Voytas,2013;Baltes and Voytas,2015).However,delivery of these nucleases using traditional transformation methods (e.g.,particle bombardment,Agrobacterium or protoplast transformation) may result in undesired genetic alterations due to random insertion of nucleaseencoding DNA intothe host genome. | Song Luo Jin Li Thomas J. Stoddard Nicholas J. Baltes Zachary L. Demorest Benjamin M. Clasen Andrew Coffman Adam Retterath Luc Mathis Daniel F. Voytas Feng Zhang | 2015 | Molecular Plant2015，8，9： | 7 |
| 6 | Targeting DNA Double-Strand Breaks with TAL Effector Nucleases显示文摘 | Christian Michelle Cermak Tomas Doyle Erin L Schmidt Clarice Zhang Feng Hummel Aaron Bogdanove Adam J Voytas Daniel F | 2010 | Genetics2010，，2： | 3 |
| 7 | The diversity of LTR retrotransposons显示文摘 | Havecker E R Gao X Voytas D F | 2004 | Genome biology2004，5，6： | 1 |
| 8 | Identification and isolation of endothelial cells based on their increased uptake of acetylate-low density lipoprotein显示文摘 | Voyta JC Via DP Butterfeild CE | 1984 | J Cell Bio1984，99，： | 1 |
| 9 | Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein 显示文摘 | Voyta JC Via DP Butterfield CE | 1984 | J Cell Biol1984，99，6： | 1 |
| 10 | A superfamily of Arabidopsis thaliana retrotransposons显示文摘 | Konieczny A Voytas DF Cummings MP | 1991 | Genetic1991，127，： | 1 |
| 11 | A copia-like transposable element family in Arabidopsis thaliana显示文摘 | Ausubel F M | 1988 | Nature1988，336，： | 1 |
| 12 | 1, 2-Dioxetances: novel chemiluminsecent enzyme substrates Applications to immunoassay显示文摘 | Brustein I Edwards B Voyta J C | 1989 | J Biolumin Chemilumin1989，4，： | 1 |
| 13 | TAL effectors: customiz- able proteins for DNA targeting 显示文摘 | Adam J Bogdanove Daniel F Voytas | 2011 | Science2011，333，6051： | 1 |
| 14 | Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein显示文摘 | Voyta JC Via DP Butterfield CE | 1984 | J Cell Biol1984，99，6： | 1 |
| 15 | Copia-like retrotransposable element evolution in diploid and polyploid cotton (Gossypium L.)显示文摘 | Pamela L. VanderWiel Daniel F. Voytas Jonathan F. Wendel | 1993 | Journal of Molecular Evolution1993，，5： | 1 |
| 16 | Copia - like retrotransposons are ubiquitous among plants 显示文摘 | VOYTAS D F CUMMINGS M P KONIECZNY A | 1992 | Proc Natl Acad Sci USA1992，89，： | 1 |
| 17 | The diversity of LTR retrotransposons显示文摘 | HAVECKER E R GAO X VOYTAS D F | | 0，，06： | 1 |
| 18 | Chemiluminescencent assay of alkaline phosphatase applied in an ultrasensitive enzyme immunoassay of thyrotropin显示文摘 | Voyta J C Thorpe G H G | 1989 | Clin Chem1989，35，： | 1 |
| 19 | Copia-like retrotransposons are ubiquitous among plants显示文摘 | Voytas D F Cummings M P Konerczny A K | 1992 | Proc NAtl Acid Sci USA1992，89，15： | 1 |
| 20 | Identification and iso- lation of endothelial cells based on their increased uptake of aeetylated-low density lipoprotein 显示文摘 | Voyta J C Viad P Butterfield C E | 1984 | J Cell Biol1984，99，6： | 1 |
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