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| 1 | 鸡氨肽酶H在大肠杆菌中的表达、纯化与部分酶学性质分析显示文摘氨肽酶H(Aminopeptidase H,APH))是生物组织内一种常见的氨基内肽酶,但因为天然材料中含量很低,基本无法深入研究其催化机理、功能与结构的关系及其在生物体内的确切功能。从鸡肝组织克隆了APH的全基因序列,并把该序列亚克隆到载体pET22b(+)上,然后转化大肠杆菌Rosetta(DE3),构建了APH的表达菌株。该菌株经IPTG诱导,在SDS-PAGE上明显出现一条与天然APH理论分子量一致的新增蛋白带,该条带的浓度随着表达时间的延长逐渐加深;6h基本达到平衡,此时重组蛋白占总蛋白的16.7%,表达水平高达94.7mg/L。对表达产物进行了活性检测、纯化和酶学性质分析,发现重组蛋白在亚基构成,热稳定性,最适pH等方面与天然APH基本相同,据此可以确认表达产物确实是APH,发酵液总活力达到1636u/L。这些结果为APH的催化机理及其在生物体内的功能的阐明奠定了重要的物质基础。 | 赖庆安 刘树滔 卢菀华 陈莉 Toshihide NISHIMURA 饶平凡 | 2008 | 生物工程学报2008，24，3： | 5 |
| 2 | Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment显示文摘 | Ai Saiga Sichi Tanabe Toshihide Nishimura | 2003 | J Agric Food Chem2003，51，： | 1 |
| 3 | Direct sequencing of peptides transferred onto polyvinylidene difluoride membrane from sodium dodecyl sulfate-polyacrylamide gel electrophoresis by electroblotting显示文摘 | Hany A El-Shemy Toshihide Nishimura Kounosuke Fujita | 2001 | Analytica Chimica Acta2001，，442： | 1 |
| 4 | Disease proteomics toward bedside reality显示文摘 | Toshihide Nishimura Atsushi Ogiwara Kiyonaga Fujii Takao Kawakami Takeshi Kawamura Hisae Anyouji Harubumi Kato | 2005 | Journal of Gastroenterology2005，，16： | 1 |
| 5 | Direct sequencing of peptides transferred onto polyvinytidene difluoride membrane from sodium dodecyt sulfate --potyacxytamide get electro phoresis by etectroblotting 显示文摘 | Hany A Et-Shemy Toshihide Nishimura Kounosuke Fujita | 2001 | Analytica Chimica Acta2001，，442： | 1 |
| 6 | Is the measurement of glycated hemoglobin A1c alone an efficient screening test for undiagnosed diabetes?显示文摘 | Tomoko Nakagami Makoto Tominaga Rimei Nishimura Nobuo Yoshiike Makoto Daimon Toshihide Oizumi Naoko Tajima | 2006 | Diabetes Research and Clinical Practice2006，，2： | 1 |
| 7 | Antioxidant activity of obtained from porcine myofibrillar proteins by protease treatment 显示文摘 | Ai Saiga Soichi Tanabe Toshihide Nishimura | 2003 | Agric Food Chem2003，51，： | 1 |
| 8 | Antioxidant activity of peptides obtained from porcine myofibrillar proteins byprotease treatment 显示文摘 | Ai Saiga Soighi Tanbae Toshihide Nishimura | 2003 | Food Chemistry2003，51，： | 1 |
| 9 | Is the measurement of glycated hemoglobin A1c alone an efficient screening test for undiagnosed diabetes?显示文摘 | Tomoko Nakagami Makoto Tominaga Rimei Nishimura Nobuo Yoshiike Makoto Daimon Toshihide Oizumi Naoko Tajima | 2006 | Diabetes Research and Clinical Practice2006，，2： | 1 |
| 10 | Taste of free amino acids and peptides显示文摘 | Toshihide Nishimura Hiromichi Kato | 1988 | Food Reviews International1988，，2： | 1 |
| 11 | Antioxidant activity of obtained from porcine myofibrillar proteins by protease treatment显示文摘 | Ai Saiga Soichi Tanabe Toshihide Nishimura | 2003 | J Agric Food Chem2003，51，： | 1 |
| 12 | Antioxidant activity of obtained from porcine myofibrillar proteinsby protease by protease treatment显示文摘 | Tanabe Soichi Nishimura Toshihide | 2003 | J Agric Food Chem2003，51，： | 1 |
| 13 | Percutaneous Radiofrequency Ablation for Pulmonary Metastases from Hepatocellular Carcinoma: Results of a Multicenter Study in Japan显示文摘 | Takao Hiraki Koichiro Yamakado Osamu Ikeda Toshiyuki Matsuoka Toshio Kaminou Takuji Yamagami Hideo Gobara Hidefumi Mimura Koichi Kawanaka Kan Takeda Yasuyuki Yamashita Yuichi Inoue Toshihide Ogawa Tsunehiko Nishimura Susumu Kanazawa | 2011 | Journal of Vascular and Interventional Radiology2011，，6： | 1 |
| 14 | Comparison of peg-interferon, ribavirin plus telaprevir vs simeprevir by propensity score matching显示文摘AIM: To compare efficacy of telaprevir(TVR) and simeprevir(SMV) combined with pegylated interferon(PEG-IFN) and ribavirin(RBV) while treating chronic hepatitis C(CHC). METHODS: In all, 306 CHC patients were included in this study. There were 159 patients in the TVR combination therapy group and 147 patients in the SMV combination therapy group. To evaluate pretreatment factors contributing to sustained virological response at 12 wk(SVR12), univariate and multivariate analyses were performed in TVR and SMV groups. To adjust for patient background between TVR and SMV groups, propensity score matching was performed. Virological response during treatment and SVR12 were evaluated.RESULTS: Overall rates of SVR12 [undetectable serum hepatitis C virus(HCV) RNA levels] were 79.2% and 69.4% in TVR and SMV groups, respectively. Patients in the SMV group were older, had higher serum HCV RNA levels, lower hemoglobin, higher prevalence of unfavorable interleukin-28B(IL28B) genotype(rs8099917), and poorer response to previous PEG-IFN and RBV treatment. Propensity score matching was performed to adjust for backgrounds(n = 104) and demonstrated SVR12 rates of 74.0% and 73.1% in the TVR and SMV groups, respectively. In the TVR group, discontinuation rates were higher because of adverse events; however, breakthrough and nonresponse was more frequent in the in SMV group. Multivariate analysis revealed IL28 B genotype(rs8099917) as the only independent predictive factor of SVR12 in both groups.CONCLUSION: SVR12 rates were almost identical following propensity score matching. | Hideki Fujii Takeshi Nishimura Atsushi Umemura Taichiro Nishikawa Kanji Yamaguchi Michihisa Moriguchi Yoshio Sumida Hironori Mitsuyoshi Chihiro Yokomizo Saiyu Tanaka Hiroki Ishikawa Kenichi Nishioji Hiroyuki Kimura Shiro Takami Yasuyuki Nagao Takayuki Takeuchi Toshihide Shima Yoshihiko Sawa Masahito Minami Kohichiroh Yasui Yoshito Itoh | 2015 | World Journal of Hepatology2015，7，28： | 1 |
| 15 | Plasma proteomics of pancreatic cancer patients by multi-dimensional liquid chromatography and two-dimensional difference gel electrophoresis (2D-DIGE): Up-regulation of leucine-rich alpha-2-glycoprotein in pancreatic cancer显示文摘 | Tatsuhiko Kakisaka Tadashi Kondo Tetsuya Okano Kiyonaga Fujii Kazufumi Honda Mitsufumi Endo Akihiko Tsuchida Tatsuya Aoki Takao Itoi Fuminori Moriyasu Tesshi Yamada Harubumi Kato Toshihide Nishimura Satoru Todo Setsuo Hirohashi | 2007 | Journal of Chromatography B2007，，1： | 1 |
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