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| 1 | Axial gradient excitation accelerates volumetric imaging of two-photon microscopy显示文摘Two-photon excitation fluorescence microscopy(TPM),owing to its capacity for subcellular resolution,intrinsic optical sectioning,and superior penetration depth in turbid samples,has revolutionized biomedical research.However,its layer-by-layer scanning to form a three-dimensional image inherently limits the volumetric imaging speed and increases phototoxicity significantly.In this study,we develop a gradient excitation technique to accelerate TPM volumetric imaging.The axial positions of the fluorophores can be decoded from the intensity ratio of the paired images obtained by sequentially exciting the specimen with two axially elongated two-photon beams of complementary gradient intensities.We achieved a 0.63μm axial localization precision and demonstrate the flexibility of the gradient TPM on various sparsely labeled samples,including bead phantoms,mouse brain tissues,and live macrophages.Compared with traditional TPM,our technique improves volumetric imaging speed(by at least sixfold),decreases photobleaching(i.e.,less than 2.07±2.89%in 25 min),and minimizes photodamages. | Yufeng Gao Xianyuan Xia Lina Liu Ting Wu Tingai Chen Jia Yu Zhili Xu Liang Wang Fei Yan Zhuo Du Jun Chu Yang Zhan Bo Peng Hui Li Wei Zheng | 2022 | Photonics Research2022，10，3： | 3 |
| 2 | Agrobacterium tu- mefaciens-mediated barley transformation 显示文摘 | TINGAY S MCELROY D KALLA Pt | 1997 | Plant Journal1997，11，6： | 1 |
| 3 | The murchisonwidefield array : the square kilometre array precursor atlow radio frequencies 显示文摘 | TINGAY SJ GOEKE R BOWMAN J D eta l | 2013 | Publications of the AstronomicalSociety of Australia2013，30，30： | 1 |
| 4 | Deepwater fold and thrust belt classification, tectonics, structure and hydrocarbon prospectivity: A review显示文摘 | C.K. Morley R. King R. Hillis M. Tingay G. Backe | 2010 | Earth Science Reviews2010，，1： | 1 |
| 5 | Effect of closed endotracheal tube suction method, catheter size, and post‐suction recruitment during high‐frequency jet ventilation in an animal model显示文摘 | J. Michele Hepponstall David G. Tingay Risha Bhatia Peter M. Loughnan Beverley Copnell | 2012 | Pediatr Pulmonol2012，，8： | 1 |
| 6 | A comparison of the effectiveness of open and closed endotracheal suction显示文摘 | Copnell B Tingay D G Kiraly N J | 2007 | Intensive Care Med2007，33，9： | 1 |
| 7 | Trends in use and outcomes of newborn infants treated with high frequency ventilation in Australia and New Zealand, 1996-2003 显示文摘 | Tingay DG Mills JF Morley C J | 2007 | Pediatric Child Heath2007，43，3： | 1 |
| 8 | 'Vertically transferred' overpressures in Brunei: Evidence for a new mecha- nism for the formation of high-magnitude overpressure 显示文摘 | Tingay M R P Hillis R R Swarbrick R E | 2007 | Geology2007，35，11： | 1 |
| 9 | Agrobacterium tumefaciens-mediated barley transformation显示文摘 | Tingay S Mcelroy D Kalla R | 1997 | Plant J1997，，11： | 1 |
| 10 | Present-day stress and neotectonics of Brunei: Implications for petroleum exploration and production显示文摘 | Tingay MRP Hillis R R Morley C K | 2009 | AAPG Bulletin2009，93，1： | 1 |
| 11 | Agrobacterium tumefaciens-mediated barley transformation显示文摘 | Sonia Tingay David McElroy Roger Kalla | | 0，，06： | 1 |
| 12 | Present-day stress ori- entation in Brunei: a snapshot of'prograding tectonics' in a Tertiary delta显示文摘 | Tingay M Hillis R Morley C et ai | 2005 | Journal of the Geological Society2005，162，1： | 1 |
| 13 | Origin of overpressure and pore pressure prediction in the Baram Delta Province,Brunei显示文摘 | Tingay M R P Hillis R R Swarbrick R E | 2009 | AAPG Bulletin2009，93，1： | 1 |
| 14 | “Vertically transferred” overpressures in Brunei:Evidence for a new mechanism for the formation of high magnitude overpressures显示文摘 | Tingay M Hillis R Swarbrick R | 2007 | Geology2007，35，11： | 1 |
| 15 | Comparison of four methods of lung volume recruitment during high frequency oscillatory ventilation显示文摘 | Pellicano A Tingay DG Mills JF | 2009 | Intensive Care Med2009，35，11： | 1 |
| 16 | Lung protective ventilation in extremely preterm infants显示文摘 | Dargaville PA Tingay DG | | 0，，09： | 1 |
| 17 | End tidal carbon dioxide is as reliable as transcutaneous monitoring in ventilated postsurgical neonates显示文摘 | Tingay DG Mun KS Perkins EJ | 2013 | Arch Dis Child Fetal Neonatal Ed2013，98，2： | 1 |
| 18 | Agrobacterium tumefaciens-mediated barley transformation显示文摘 | Tingay S McElroy Kalla R | 1997 | Plant J1997，11，： | 1 |
| 19 | Origin of overpressure and pore-pressure prediction in the Baram Province, Brunei显示文摘 | Tingay M R P Hillis R R Swarbrick R E | 2009 | AAPG Bulletin2009，93，1： | 1 |
| 20 | The effects of endotracheal suction on regional tidal ventilation and end-expiratory lung volume显示文摘 | Tingay D G Copnell B Grant C A | 2010 | Intensive Care Med2010，36，5： | 1 |
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