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| 1 | IL28B polymorphism and cytomegalovirus predict response to treatment in Egyptian HCV type 4 patients显示文摘AIM:To test whether the status of positive cytomegalovirus(CMV) DNA detection adds to the predictive value of IL28B and to further categorize C/T allele carriers.METHODS:This study included 166 chronic hepatitis C(CHC) patients who received combined interferon and ribavirin therapy for 48 wk,84 spontaneous hepatitis C virus(HCV) resolvers who were positive for IgG anti-HCV antibody and negative for HCV RNA,and 100 healthy subjects who were negative for both HCV antibodies and RNA as controls.Genomic DNA from peripheral blood was used for IL28B rs.12979860 single nucleotide polymorphism(SNP) and CMV DNA detection.A 139 bp fragment containing IL28B SNP was amplified in all subjects by polymerase chain reaction using a specifically designed primer.Then the IL28B rs.12979860 SNP was detected by restriction fragment length polymorphism(RFLP) genotyping.The presence of CMV DNA was tested by amplification of the gB1 gene using nested polymerase chain reaction.The role of CMV and IL28B rs.12979860 SNP genotypes in determining the response rate to combined interferon therapy and clinical status of patients were statistically analyzed.RESULTS:Current data showed that 67% of patients carrying the IL28B 12979860 C/C allele had a sustained viral response(SVR) while the genotypes C/T and TT were associated with lower SVR rates,50% and 48%,respectively.SVR rates for the C/C allele were lower than other HCV genotypes and/or other populations.Genotype CC was associated with the response to interferon(P = 0.025).Genotype C/C was reduced from 48% in controls to 14% in CHC patients suggesting its protective role against progression to chronicity.The majority of spontaneously cleared subjects(86%) were C/C,confirming its protective role.The C/T allele was present in 71% of CHC patients compared with 38% of controls,so the use of IL28B SNP genotyping only in these patients may be of little value as a predictor of response.CMV reactivation occurred in 40% of CHC patients.Co-infection with CMV seriously diminished the response to interferon(IFN) therapy,with SVR rates in C/C genotypes 87.5% in CMV-negative patients and 12.5% in CMV-positive patients(P < 0.0001).SVR rates among C/T carriers were reduced to < 50% in patients with positive CMV DNA while the non-response rate doubled.These data indicate that a supplemental assay for CMV viremia adds to the prognostic value of IL28B genotyping.CONCLUSION:The results suggest that both genetic(i.e.,spontaneous) and therapeutic(IFN-based therapy) arms are complementary in the battle against HCV.CMV DNA testing may be of value to better predict the response to IFN,particularly in IL28B C/T carriers. | Mostafa K El Awady Noha G Bader El Din Ashraf Tabll Yaser El Hosary Ashraf O Abdel Aziz Hesham El Khayat Mohsen Salama Tawfeek H Abdelhafez | 2013 | World Journal of Gastroenterology2013，19，2： | 8 |
| 2 | Flow cytometric detection of hepatitis C virus antigens in infected peripheral blood leukocytes: Binding and entry显示文摘AIM: We designed two synthetic-core-specific peptides core 1 (C1) and core 2 (C2), and an E1-specific peptide (E1). We produced specific polyclonal antibodies againstthese peptides and used the antibodies for detection of HCV antigens on surface and within infected peripheral blood leukocytes.METHODS: Peripheral blood from a healthy individual who tested negative for HCV RNA was incubated with HCV type 4 infected serum for 1 h and 24 h at 37 ℃. Cells were stained by direct and indirect immunofluorescence and measured by flow cytometry.RESULTS: After 1 h of incubation, antibodies against C1,C2, and E1 detected HCV antigens on the surface of 27%,26% and 73% of monocytes respectively, while 10%, 5% and 9% of lymphocytes were positive with anti-C1, anti-C2 and anti-E1 respectively. Only 1-3% of granulocytes showed positive staining with anti-C1, anti-C2 and anti E1 antibodies. After 24 h of incubation, we found no surface staining with anti-C1, anti-C2 or anti-E1. Direct immunostaining using anti-C2 could not detect intracellular HCV antigens, after 1 h of incubation with the virus, while after 24 h of incubation, 28% of infected cells showed positive staining. Only plus strand RNA was detectable intracellularly as early as 1 h after incubation, and remained detectable throughout 48 h post-infection.Interestingly, minus RNA strand could not be detected after 1 h, but became strongly detectable intracellularly after 24 h post-infection.CONCLUSION: Monocytes and lymphocytes are the preferred target cells for HCV infection in peripheral blood leukocytes. Our specific anti-core and anti-E1 antibodies are valuable reagents for demonstration of HCV cell cycle.Also, HCV is capable of infecting and replicating in peripheral blood mononuclear cells as confirmed by detection of minus strand HCV RNA as well as intracellular staining of core HCV antigen. | Mostafa K El-Awady Ashraf A Tabll El-Rashdy M Redwan Samar Youssef Moataza H Omran Fouad Thakeb Maha El-Demellawy | 2005 | World Journal of Gastroenterology2005，11，33： | 4 |
| 3 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 4 | DNA ploidy of biopsies from patients with liver cirrhosis and hepatocellular carcinoma: a flow cytometric analysis显示文摘 | Attallah AM Tabll AA Salem SF | 1999 | Cancer Lett1999，142，1： | 1 |
| 5 | Dysregulation of blood lymphocyte subsets and natural killer cells in schistosoml liver cirrhosis and hepatocellular carcinoma显示文摘 | Attallah AM Tabll AA El-Sadany M | 2003 | Clin Exp Med2003，3，3： | 1 |
| 6 | Monoclonal antibodies:Principles and applications of immmunodiagnosis and immunotherapy for hepatitis C virus显示文摘Hepatitis C virus(HCV) is a major health problem worldwide. Early detection of the infection will help better management of the infected cases. The monoclonal antibodies(m Ab) of mice are predominantly used for the immunodiagnosis of several viral,bacterial,and parasitic antigens. Serological detection of HCV antigens and antibodies provide simple and rapid methods of detection but lack sensitivity specially in the window phase between the infection and antibody development. Human mA b are used in the immunotherapy of several blood malignancies,such as lymphoma and leukemia,as well as for autoimmune diseases. In this review article,we will discuss methods of mouse and human monoclonal antibody production. We will demonstrate the role of mouse mA b in the detection of HCV antigens as rapid and sensitive immunodiagnostic assays for the detection of HCV,which is a major health problem throughout the world,particularly in Egypt. We will discuss the value of HCV-neutralizing antibodies and their roles in the immunotherapy of HCV infections and in HCV vaccine development. We will also discuss the different mechanisms by which the virus escape the effect of neutralizing mA b. Finally,we will discuss available and new trends to produce antibodies,such as egg yolk-based antibodies(Ig Y),production in transgenic plants,and the synthetic antibody mimics approach. | Ashraf Tabll Aymn T Abbas Sherif El-Kafrawy Ahmed Wahid | 2015 | World Journal of Hepatology2015，7，22： | 1 |
| 7 | Establishment of human clones producing neutralizing humanmonoclonal antibodies to the envelope E1/E2 protein of hepatitis C virus by EBVimmortalization of immune CD22+ B cells显示文摘 | Ashraf Tabll Yasmine El Abd Noha G. Bader El Din Reem El Shenawy Tawfeek H. Abdelhafez Mostafa El Awady Hanan El-Mohamady Sergei Viazov | | 2014 (3,4)0，，： | 1 |
| 8 | MOUSE MONOCLONAL ANTIBODY TOWARDS E1 SPECIFIC EPITOPE BLOCKS VIRAL ENTRY AND INTRACELLULAR VIRAL REPLICATION IN VITRO显示文摘 | AshrafA. Tabll RehabI. Moustafa YasmineS. El Abd NohaG. Bader El Din Reem El-Shenawy Hassan Yousef Manal Hussein RehamM. Dawood MoatazaH. Omran MostafaK. El-Awady | 2014 | Journal of Immunoassay and Immunochemistry2014，，1： | 1 |
| 9 | Establishment of human clones producing neutralizing human monoclonal antibodies to the envelope E l/E2 protein of hepatitis C virus by EBV immortalization of immune CD22+ B cells显示文摘 | Tabll A E1 Abd Y El Din NG | 2013 | Hum Antibodies2013，22，： | 1 |
| 10 | AgNORs count and DNA ploidy in liver biopsies from patients with schistosomal liver cirrhosis and hepatocellular carcinoma 显示文摘 | Attallab A M Tabll A A E1 - Nashar E | 2009 | Clinical Biochemistry2009，42，1617： | 1 |
| 11 | Dysregulation of blood lymphocyte subsets and natural killer cells in schistosomal liver cirrhosis and hepatocellular carcinoma显示文摘 | Attallah AM Tabll AA El-Sadany M | 2003 | Clin Exp Med2003，3，3： | 1 |
| 12 | Potential activity of camel milk-amylase and lactoferrin against hepatitis C virus infectivity in HepG2 and lymphocytes显示文摘 | EL-FAKHARANY E M TABLL A EI-WAHAB A A | 2008 | Hepatitis Monthly2008，8，2： | 1 |
| 13 | Conserved pep-tides within the E2 region of hepatitis C virus induce humoraland cellular responses in goats显示文摘 | El-Awady M K Tabll A A Yasmine S | 2009 | Virol J2009，6，： | 1 |
| 14 | Establishment of hybrid cell lines producing monoclonal antibodies to a synthetic Peptide from the el region of the hepatitis C virus 显示文摘 | Tabll AA Khalil SB El-Shenawy RM | 2008 | Immunoassay Immunochem2008，29，1： | 1 |
| 15 | DNA ploidy and liver cell dysplasia in liver biopsies from patients with liver cirrhosis显示文摘 | El-Sayed SS El-Sadany M Tabll AA | 2004 | Can J Gastroenterol2004，18，2： | 1 |
| 16 | Potential activity in HepG2 and lymphocytes 显示文摘 | E1-Fakharany EM Tabll A Ei-Wahab AA | 2008 | Hepatitis Monthly2008，8，2： | 1 |
| 17 | Therapeutic Perspectives of IL1 Family Members in Liver Diseases:An Update显示文摘Interleukin(IL)1 superfamily members are a cornerstone of a variety of inflammatory processes occurring in various organs including the liver.Progression of acute and chronic liver diseases regardless of etiology depends on the stage of hepatocyte damage,the release of inflammatory cytokines and disturbances in gut microbiota.IL1 cytokines and re-ceptors can have pro-or anti-inflammatory roles,even dual functionalities conditioned by the microenvironment.Devel-oping novel therapeutic strategies to block the IL1/IL1R sign-aling pathways seems like a reasonable option.This mode of action is now exploited by anakinra and canakinumab,which are used to treat different inflammatory illnesses,and studies in liver diseases are on the way.In this mini review,we have focused on the IL1 superfamily members,given their cru-cial role in liver inflammation diseases,specifically discussing their potential role in developing new treatment strategies. | Ines Bilić Ćurčić Tomislav Kizivat Ana Petrović Robert Smolić Ashraf Tabll George Y.Wu Martina Smolić | 2022 | Journal of Clinical and Translational Hepatology2022，10，6： | 0 |
| 18 | Antibody to El peptide of hepatitis C virus genotype 4 inhibits virus binding and entry to HepG2 cells in vitro显示文摘瞄准:对丙肝病毒(HCV ) 的 E1 区域分析抗体的抵销的活动。特定的 polyclonal 抗体与从 HCV 的 E1 区域被导出并且被显示高度在 HCV 之中被保存的合成的肽经由新西兰兔子的免疫被提起出版遗传型。方法:超 HCV E1 抗体与为 HCV RNA 积极的浆液样品在 4 度摄氏在夜里被孵化,与从 615 ~ 3.2 百万 IU/ mL 的病毒的负担。对待的重量的单位一为 90 min 与 HepG2 房间被孵化。由反 E1 抗体病毒的绑定和入口堵住进房间借助于 RT-PCR 和流动血细胞计数被测试。结果:用 FITC 染色的直接免疫结合了分析显示出的流动 cytometric 跟随的 E1 抗体在样品的减少的吝啬的荧光紧张与未经治疗的样品相比与 E1 抗体预先孵化。而且, 13 从 18 积极重量的单位一(72%) 显示出由 RT-PCR 检测了的传染性的完全的抑制。结论:在房子里生产 E1 抗体,块绑定和到在病毒绑定建议这 epitope 的参与的靶细胞的 HCV virion 感染的入口和入口。堵住病毒附件到人的房间的这些抗体的隔离作为治疗学的试剂有用。 | Mostafa K EL-Awady Ashraf A Tabll Khaled Atef Samar S Yousef Moataza H Omran Yasmin EI-Abd Noha G Bader-Eldin Ahmad M Salem Samir F Zohny Wael T EI-Garf | 2006 | World Journal of Gastroenterology2006，12，16： | 0 |
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