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您的检索式：作者名="THOMAS HUSER"
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| 1 | Deep-learning based denoising and reconstruction of super-resolution structured illumination microscopy images显示文摘Super-resolution structured illumination microscopy(SR-SIM) provides an up to twofold enhanced spatial resolution of fluorescently labeled samples. The reconstruction of high-quality SR-SIM images critically depends on patterned illumination with high modulation contrast. Noisy raw image data(e.g., as a result of low excitation power or low exposure time), result in reconstruction artifacts. Here, we demonstrate deep-learning based SR-SIM image denoising that results in high-quality reconstructed images. A residual encoding–decoding convolutional neural network(RED-Net) was used to successfully denoise computationally reconstructed noisy SR-SIM images.We also demonstrate the end-to-end deep-learning based denoising and reconstruction of raw SIM images into high-resolution SR-SIM images. Both image reconstruction methods prove to be very robust against image reconstruction artifacts and generalize very well across various noise levels. The combination of computational image reconstruction and subsequent denoising via RED-Net shows very robust performance during inference after training even if the microscope settings change. | ZAFRAN HUSSAIN SHAH MARCEL MÜLLER TUNG-CHENG WANG PHILIP MAURICE SCHEIDIG AXEL SCHNEIDER MARK SCHÜTTPELZ THOMAS HUSER WOLFRAM SCHENCK | 2021 | Photonics Research2021，9，5： | 11 |
| 2 | High-contrast,fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser显示文摘Coherent Raman scattering(CRS)microscopy is widely recognized as a powerful tool for tackling biomedical problems based on its chemically specific label-free contrast,high spatial and spectral resolution,and high sensitivity.However,the clinical translation of CRS imaging technologies has long been hindered by traditional solid-state lasers with environmentally sensitive operations and large footprints.Ultrafast fibre lasers can potentially overcome these shortcomings but have not yet been fully exploited for CRS imaging,as previous implementations have suffered from high intensity noise,a narrow tuning range and low power,resulting in low image qualities and slow imaging speeds.Here,we present a novel high-power self-synchronized two-colour pulsed fibre laser that achieves excellent performance in terms of intensity stability(improved by 50 dB),timing jitter(24.3 fs),average power fluctuation(<0.5%),modulation depth(>20 dB)and pulse width variation(<1.8%)over an extended wavenumber range(2700-3550 cm^(−1)).The versatility of the laser source enables,for the first time,high-contrast,fast CRS imaging without complicated noise reduction via balanced detection schemes.These capabilities are demonstrated in this work by imaging a wide range of species such as living human cells and mouse arterial tissues and performing multimodal nonlinear imaging of mouse tail,kidney and brain tissue sections by utilizing second-harmonic generation and twophoton excited fluorescence,which provides multiple optical contrast mechanisms simultaneously and maximizes the gathered information content for biological visualization and medical diagnosis.This work also establishes a general scenario for remodelling existing lasers into synchronized two-colour lasers and thus promotes a wider popularization and application of CRS imaging technologies. | Cihang Kong Christian Pilger Henning Hachmeister Xiaoming Wei Tom H.Cheung Cora S.W.Lai Nikki P.Lee Kevin K.Tsia Kenneth K.Y.Wong Thomas Huser | 2020 | Light(Science & Applications)2020，9，1： | 5 |
| 3 | Mechanism-Based Facilitated Maturation of Human Pluripotent Stem Cell–Derived Cardiomyocytes显示文摘 | Deborah K. Lieu Ji-Dong Fu Nipavan Chiamvimonvat Kelvin Chan Tung Gregory P. McNerney Thomas Huser Gordon Keller Chi-Wing Kong Ronald A. Li | 2013 | Circulation: Arrhythmia and Electrophysiology2013，，： | 1 |
| 4 | Characterization of an acetate-decarboxylating, non-hydrogen-oxidizing methane bacterium显示文摘 | Alexander J. B. Zehnder Beat A. Huser Thomas D. Brock Karl Wuhrmann | 1980 | Archives of Microbiology1980，，1： | 1 |
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