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| 1 | European Consensus Guidelines on the Management of Neonatal Respiratory Distress Syndrome in Preterm Infants - 2013 Update显示文摘 | Sweet David G Carnielli Virgilio Greisen Gorm Hallman Mikko Ozek Eren Plavka Richard Saugstad Ola D Simeoni Umberto Speer Christian P Vento Maximo Halliday Henry L | 2013 | Neonatology2013，，4： | 3 |
| 2 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 3 | Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration显示文摘AIM:To develop a model of stress-induced senescence to study the hepatocyte senescence associated secretory phenotype(SASP).METHODS:Hydrogen peroxide treatment was used to induce senescence in the human Hep G2 hepatocyte cell line.Senescence was confirmed by cytochemical staining for a panel of markers including Ki67,p21,heterochromatin protein 1β,and senescence-associated-β-galactosidase activity.Senescent hepatocytes were characterised by gene expression arrays and quantitative polymerase chain reaction(q PCR),and conditioned media was used in proteomic analyses,a human chemokine protein array,and cell migration assays to characterise the composition and function of the hepatocyte SASP.RESULTS:Senescent hepatocytes induced classical markers of senescence(p21,heterochromatin protein1β,and senescence-associated-β-galactosidase activity);and downregulated the proliferation marker,Ki67.Hepatocyte senescence induced a 4.6-fold increase in total secreted protein(P=0.06)without major alterations in the protein profile.Senescence-induced genes were identified by microarray(Benjamini Hochbergcorrected P<0.05);and,consistent with the increase in secreted protein,gene ontology analysis revealed a significant enrichment of secreted proteins among inducible genes.The hepatocyte SASP included characteristic factors such as interleukin(IL)-8 and IL-6,as well as novel components such as SAA4,IL-32and Fibrinogen,which were validated by q PCR and/or chemokine protein array.Senescent hepatocyteconditioned medium elicited migration of inflammatory(granulocyte-macrophage colony stimulating factor,GM-CSF-derived),but not non-inflammatory(CSF-1-derived)human macrophages(P=0.022),which could contribute to a pro-inflammatory microenvironment in vivo,or facilitate the clearance of senescent cells.CONCLUSION:Our novel model of hepatocyte senescence provides insights into mechanisms by which senescent hepatocytes may promote chronic liver disease pathogenesis. | Katharine M Irvine Richard Skoien Nilesh J Bokil Michelle Melino Gethin P Thomas Dorothy Loo Brian Gabrielli Michelle M Hill Matthew J Sweet Andrew D Clouston Elizabeth E Powell | 2014 | World Journal of Gastroenterology2014，20，47： | 2 |
| 4 | Fathers and breast feeding very-low- birthweight preterm babies显示文摘 | Sweet L Darbyshire P | 2009 | Midwifery2009，25，： | 1 |
| 5 | Flame-excited luminescence in the oxides Ta2OsNb2Os, TiO2, ZnO, and SnO2显示文摘 | PROVENZANO P L JINDAL G R SWEET J a | 2001 | J Lumin2001，92，4： | 1 |
| 6 | Complete ^1H and ^13C-NMR spectra of pregnenlolne 显示文摘 | Szendi Z Forg P Sweet F | 1995 | Steroids1995，60，： | 1 |
| 7 | Bacterial/CpG-DNA down-modulates colony stimulating factor-1 receptor surface expression on murine bone marrow-derived macrophages with concomitant growth are stand factor-independent survival显示文摘 | Sester D P Beasley S J Sweet M J | 1999 | J Immunol1999，16，3： | 1 |
| 8 | Detecting simulated amnesia forauto blographicaland recently learned information usingthe P300 event- ralated potential显示文摘 | Ellwanger J Rosenfeld J P Sweet J J | 1996 | International Journal of Psychophysiol- ogy1996，23，12： | 1 |
| 9 | Prevalence of deliberate self harm and attempted suicide within contemporary Goth youth subculture: longitudinal cohort study显示文摘 | Young R Sweeting H West P | 2006 | BMJ2006，332，7549： | 1 |
| 10 | The safety profile of the varicella vaccine:a 10-year review显示文摘 | GALEA S A SWEET A BENINGER P | 2008 | J Infect Dis2008，197，2： | 1 |
| 11 | Factors influen- cing cognitive function, sleep, and quality of life in indi- viduals with systemic lupus erythematosus:A review of the literature 显示文摘 | Sweet J J Doninger N A Zee P C | 2004 | Clin Neuropsychol2004，18，1： | 1 |
| 12 | Missed opportunities for noninvasive positive pressure ventilation: a utilization review 显示文摘 | Sweet D D Naismith A Keenan S P | 2008 | J Crit Care2008，23，1： | 1 |
| 13 | Kinetics of cyclization of vinyl ehloride-metyl methacrylate copolymes 显示文摘 | SWEET G E BELL J P | 1972 | Polym Sci Polym Chem1972，10，： | 1 |
| 14 | Dynamic contrast-enhanced magnetic resonance imaging for localization of recurrent prostate cancer after external beam radiotherapy 显示文摘 | lqalder MA Chung P Sweet J | 2008 | Int J Radiat Oncol Biol Phys2008，70，： | 1 |
| 15 | Face, conten and con- struct validity of the University of Washington virtual reality transurethral prostate resection trainer显示文摘 | Sweet R Kowalewski T Oppenheimer P | 2004 | J Urol2004，172，51： | 1 |
| 16 | Detecting Simulated Amnesia for Autobiographical and Re cently Learned Information Using the P300 Event Related Potential 显示文摘 | ELLWANGER J ROSENFELD J P SWEET J J | 1996 | International Journal of Psychophysiology1996，23，： | 1 |
| 17 | Prevalence of asthma symptoms among adults aged 20-44 years in Canada显示文摘 | Manfreda J Becklake MR Sears MR Chan-Yeung M Dimich-Ward H Siersted HC Ernst P Sweet L Van Til L Bowie DM Anthonisen NR Tate RB | 2001 | CMAJ2001，164，7： | 1 |
| 18 | The postmarketing safety profile of varicella vaccine 显示文摘 | Sharrar RG LaRussa P Galea SA Steinberg SP Sweet AR Keatley RM WellsME Stephenson WP Gershon AA | 2000 | Vaccine2000，19，78： | 1 |
| 19 | Verbal working memory and atherosclerosis in patients with cardiovascular disease: an fMRI study显示文摘 | Haley A P Sweet L H Gunstad J | 2007 | Neuroimaging2007，17，： | 1 |
| 20 | Quantitative analysis by various GLC response-factor theories for partially methylated and partially ethylated alditol acetates显示文摘 | SWEET D P SHAPIRO R H ALERSHEIM P | 1975 | Carbohydr Res1975，40，： | 1 |
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