|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Genetic analysis of 'all-fish' growth hormone gene transferred carp (Cyprinus carpio L.) and its F_1 generation显示文摘Recornbinant 'all-fish' growth hormone gene (GH) was microinjected into the fertilized eggs of carp. A comparison between the growth traits of transgenics and non-transgenics was carried out, and the transgenic individuals with significant 'fast-growing' effect were successfully gained. A comparison on the reproductivities was also given out between the transgenics and their non-transgenic siblings, and showed that the reproductive capacity of transgenics was substantially equivalent to those of the non-transgenics. On the other hand, the genetic separation and the characteristic distribution of the FI generation were genetically analyzed, which gave solid evidence for the hypothesis that 2-3 chromosomes are integrated with trans-gene. In addition, the distinct biological effects for multi-site-integrated transgenes were further discussed. The present study opens a door for the breeding of 'fast-growing' transgenic fish. | WANG Yaping, HU Wei, WU Gang,SUN Yonghua, CHEN Shangping, ZHANG Fuying,ZHU Zuoyan, FENG Jianxin & ZHANG Xirui1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 2. Henan Institute of Aquaculture, Zhengzhou 450044, China | 2001 | Chinese Science Bulletin2001，46，14： | 23 |
| 2 | Myelin Oligodendrocyte Glycoprotein-IgG Contributes to Oligodendrocytopathy in the Presence of Complement, Distinct from Astrocytopathy Induced by AQP4-IgG显示文摘Immunoglobulin G against myelin oligodendrocyte glycoprotein(MOG-Ig G) is detectable in neuromyelitis optica spectrum disorder(NMOSD) without aquaporin-4 Ig G(AQP4-Ig G), but its pathogenicity remains unclear.In this study, we explored the pathogenic mechanisms of MOG-Ig G in vitro and in vivo and compared them with those of AQP4-Ig G. MOG-Ig G-positive serum induced complement activation and cell death in human embryonic kidney(HEK)-293 T cells transfected with human MOG. In C57 BL/6 mice and Sprague-Dawley rats, MOG-Ig G only caused lesions in the presence of complement. Interestingly, AQP4-Ig G induced astroglial damage, while MOGIg G mainly caused myelin loss. MOG-Ig G also induced astrocyte damage in mouse brains in the presence ofcomplement. Importantly, we also observed ultrastructural changes induced by MOG-Ig G and AQP4-Ig G. These findings suggest that MOG-Ig G directly mediates cell death by activating complement in vitro and producing NMOSDlike lesions in vivo. AQP4-Ig G directly targets astrocytes,while MOG-Ig G mainly damages oligodendrocytes. | Ling Fang Xinmei Kang Zhen Wang Shisi Wang Jingqi Wang Yifan Zhou Chen Chen Xiaobo Sun Yaping Yan Allan G. Kermode Lisheng Peng Wei Qiu | 2019 | Neuroscience Bulletin2019，35，5： | 13 |
| 3 | Phosphorus oxoanion-intercalated layered double hydroxides for high-performance oxygen evolution显示文摘合理设计和为氧进化反应(OER ) 的有效、划算的电极的控制制造为探讨崭新的精力危机是批评的。有特定的夹层阴离子的镍铁分层的双氢氧化物(NiFe-LDHs )( 即磷酸盐,亚磷酸盐,和次磷酸盐) 被一个一起沉淀方法制作并且作为氧进化 electrocatalysts 调查了。磷 oxoanion 的置闰在一个碱的答案提高了 OER 活动;最佳的表演(即, 215 mV, 37.7 mV/dec 的一个小 Tafel 斜坡,和稳定的电气化学的行为的一个低发作潜力) 与设置次磷酸盐的 NiFe-LDH 催化剂被完成,在传统的设置碳酸盐的 NiFe-LDH 上的示威戏剧的改进以活动和耐久性。这提高的表演被归因于在设置的磷的 oxoanions 和分享边的瞬间 6 之间的相互作用(M = Ni, Fe ) 层,它修改表面 Ni 地点的电子结构。这个概念应该为更有效的基于 LDH 的氧进化 electrocatalysts 的设计是鼓舞人心的。 | Ma Luo Zhao Cai Cheng Wang Yongmin Bi Li Qian Yongchao Hao Li Li Yun Kuang Yaping Li Xiaodong Lei Ziyang HUO Wen Liu Hailiang Wang Xiaoming Sun Xue Duan | 2017 | Nano Research2017，10，5： | 12 |
| 4 | Global Dynamic Molecular Profiling of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing显示文摘The regulation of stomatal lineage cell development has been extensively investigated.However,a comprehensive characterization of this biological process based on single-cell transcriptome analysis has not yet been reported.In this study,we performed RNA sequencing on 12844 individual cells from the cotyledons of 5-day-old Arabidopsis seedlings.We identified 11 cell clusters corresponding mostly to cells at specific stomatal developmental stages using a series of marker genes.Comparative analysis of genes with the highest variable expression among these cell clusters revealed transcriptional networks that regulate development from meristemoid mother cells to guard mother cells.Examination of the developmental dynamics of marker genes via pseudo-time analysis revealed potential interactions between these genes.Collectively,our study opens the door for understanding how the identified novel marker genes participate in the regulation of stomatal lineage cell development. | Zhixin Liu Yaping Zhou Jinggong Guo Jiaoai Li Zixia Tian Zhinan Zhu Jiajing Wang Rui Wu Bo Zhang Yongjian Hu Yijing Sun Yan Shangguan Weiqiang Li Tao Li Yunhe Hu Chenxi Guo Jean-David Rochaix Yuchen Miao Xuwu Sun | 2020 | Molecular Plant2020，13，8： | 11 |
| 5 | High efficient gene targeting in rice field eel Monopterus albus by transcription activator-like effector nucleases显示文摘Genome editing plays an important role in the research of gene functions,gene correction and cell replacement therapy.Traditionally,genome editing is mainly dependent on the technology of homologous recombination(HR)and embryonic stem(ES)cell lines in mammals[1].However,genome editing is severely limited by the low efficiency of HR and lack of ES cell lines in most organisms.Recently,the development of zinc finger nucleases(ZFNs), | Ke Feng Hongrui Luo Yongming Li Ji Chen Yaping Wang Yonghua Sun Zuoyan Zhu Wei Hu | 2017 | Science Bulletin2017，62，3： | 9 |
| 6 | Characterization and Fine Mapping of a Novel Rice Narrow Leaf Mutant nal9显示文摘A narrow leaf mutant was isolated from transgenic rice(Oryza sativa L.)lines carrying a T-DNA insertion.The mutant is characterized by narrow leaves during its whole growth period,and was named nal9(narrow leaf 9).The mutant also has other phenotypes,such as light green leaves at the seedling stage,reduced plant height,a small panicle and increased tillering.Genetic analysis revealed that the mutation is controlled by a single recessive gene.A hygromycin resistance assay showed that the mutation was not caused by T-DNA insertion,so a map-based cloning strategy was employed to isolate the nal9 gene.The mutant individuals from the F2generations of a cross between the nal9 mutant and Longtepu were used for mapping.With 24 F2mutants,the nal9 gene was preliminarily mapped near the marker RM156 on the chromosome 3.New INDEL markers were then designed based on the sequence differences between japonica and indica at the region near RM156.The nal9 gene was fnally located in a 69.3 kb region between the markers V239B and V239G within BAC OJ1212_C05 by chromosome walking.Sequence and expression analysis showed that an ATP-dependent Clp protease proteolytic subunit gene(ClpP)was most likely to be the nal9 gene.Furthermore,the nal9 mutation was rescued by transformation of the ClpP cDNA driven by the 35S promoter.Accordingly,the ClpP gene was identifed as the NAL9 gene.Our results provide a basis for functional studies of NAL9 in future work. | Wei Li Chao Wu Guocheng Hu Li Xing Wenjing Qian Huamin Si Zongxiu Sun Xingchun Wang Yaping Fu Wenzhen Liu | 2013 | Journal of Integrative Plant Biology2013，55，11： | 8 |
| 7 | Layered double hydroxides with atomic-scale defects for superior electrocatalysis显示文摘原子作文调节和缺点工程是有效策略 toenhance 由 improvingthe 的 multicomponent 催化剂的催化表演合作效果;然而,戏剧性地通过同时的缺点 engineeringat 在 multicomponent 材料上调节活跃地点仍然保持挑战性原子规模因为本地环境的类似。此处,把氧进化反应(OER ) 用作探查反应,我们 deliberatelyintroduced 底可溶的 Zn (II ) 或艾尔(III ) 地点进 NiFe 分层的双氢氧化物(LDH ) ,它是最好的 OER 催化剂之一。然后, Zn (II ) 或 siteswere 有选择地蚀刻创造原子 M (II ) 的艾尔(III )/M (III ) 背叛,哪个 dramaticallyenhanced OER 活动。在 20 妈的当前的密度?? | Oixian Xie Zhao Cai Pengsong Li Daojin Zhou Yongmin Bi Xuya Xiong Enyuan Hu Yaping Li Yun Kuang Xiaoming Sun | 2018 | Nano Research2018，11，9： | 8 |
| 8 | Influence of chromium modification on the properties of MnO_x-FeO_x catalysts for the low-temperature selective catalytic reduction of NO by NH_3显示文摘Catalytic properties of MnOx -FeOx complex oxide (hereafter denoted as Mn-Fe) catalysts modified with different loadings of chromium oxide were investigated by using the combination of physico-chemical techniques, such as N2 physisorption, X-ray diffraction (XRD), high-resolution transmission electron microscope (HRTEM), in situ Fourier transform infrared spectroscopy (in situ FT-IR) and temperature-programmed reduction (TPR) and their catalytic activities were evaluated with the selective catalytic reduction (SCR) of NOx by NH3 . It was found that with the addition of Cr, more NO could be removed in the low-temperature window (below 120℃). Among the tested catalysts, Mn-Fe-Cr (2 : 2 : 1) catalyst exhibited the best catalytic performance at 80℃ with the NO conversion higher than 90%. The combination of the reaction and characterization results indicated that (1) the strong interaction among tertiary metal oxides existed in the catalysts when Cr was appropriately added, which made the active components better dispersed with less agglomeration and sintering and the largest BET specific surface area could be obtained; (2) Cr improved the low-temperature reducibility of the catalyst and promoted the formation of the active intermediate (-NH+3 ), which favored the low-temperature SCR reaction. | Kai Shen Yaping Zhang Xiaolei Wang Haitao Xu Keqin Sun Changcheng Zhou | 2013 | Journal of Energy Chemistry2013，22，4： | 7 |
| 9 | Review of Chinese atmospheric science research over the past 70 years: Synoptic meteorology显示文摘Synoptic meteorology is a branch of meteorology that uses synoptic weather observations and charts for the diagnosis,study,and forecasting of weather.Weather refers to the specific state of the atmosphere near the Earth’s surface during a short period of time.The spatial distribution of meteorological elements in the atmosphere can be represented by a variety of transient weather phenomena,which are caused by weather systems of different spatial and temporal scales.Weather is closely related to people’s life,and its development and evolution have always been the focus of atmospheric scientific research and operation.The development of synoptic meteorology is closely related to the development of observation systems,dynamical theories and numerical models.In China,observation networks have been built since the early 1950 s.Up to now,a comprehensive meteorological observation systembased on ground,air and space has been established.In particular,the development of a new generation of dense radar networks,the development of the Fengyun satellite series and the implementation of a series of large field experiments have brought our understanding of weather from large-scale environment to thermal dynamics,cloud microphysical structure and evolution characteristics of meso and micro-scale weather systems.The development of observation has also promoted the development of theory,numerical model and simulation.In the early days,China mainly used foreign numerical models.Lately,China has developed numerical model systems with independent intellectual property rights.Based on the results of high-resolution numerical simulations,in-depth understanding of the initiation and evolution mechanism and predictability of weather at different scales has been obtained.Synoptic meteorology has gradually changed from an initially independent development to a multidisciplinary approach,and the interaction between weather and the change of climate and environment has become a hot and frontier topic in atmospheric science.This paper reviews the important scientific and technological achievements made in China over the past 70 years in the fields of synoptic meteorology based on the literatures in China and abroad,from six aspects respectively including atmospheric dynamics,synoptic-scale weather,typhoon and tropical weather,severe convective weather,numerical weather prediction and data assimilation,weather and climate,atmospheric physics and atmospheric environment. | Zhiyong MENG Fuqing ZHANG Dehai LUO Zhemin TAN Juan FANG Jianhua SUN Xueshun SHEN Yunji ZHANG Shuguang WANG Wei HAN Kun ZHAO Lei ZHU Yongyun HU Huiwen XUE Yaping MA Lijuan ZHANG Ji NIE Ruilin ZHOU Sa LI Hongjun LIU Yuning ZHU | 2019 | Science China Earth Sciences2019，62，12： | 7 |
| 10 | Effects of redox-active interlayer anions on the oxygen evolution reactivity of NiFe-layered double hydroxide nanosheets显示文摘镍铁分层的双氢氧化物(NiFe-LDH ) nanosheets 显示出最佳的氧进化反应(OER ) 性能;然而,在 OER 活动的设置的离子的角色仍然保持不清楚。在这个工作,我们证明 NiFe-LDHs 的活动能被设置的阴离子与不同氧化还原作用潜力定制。而 NiFe-LDHs 与高氧化还原作用潜力(低减少能力)的阴离子设置了,有低氧化还原作用潜力(高减少能力)的阴离子的置闰例如次磷酸盐,与 240 mV 的低 OER 过电位和 36.9 mV/dec 的一个小 Tafel 斜坡导致 NiFe-LDHs ,例如 fluorion ,显示出 370 mV 的高过电位和 80.8 mV/dec 的一个 Tafel 斜坡。OER 活动与标准氧化还原作用潜力显示出令人吃惊的线性关联。密度功能的理论计算和 X 光检查光电子光谱学分析显示设置的阴离子改变在表面暴露了的金属原子的电子结构。有低标准氧化还原作用潜力和强壮的减少能力的阴离子把更多的电子转移到氢氧化物层。这增加表面金属地点的电子密度并且稳定他们的高原子价的状态,其形成在 OER 过程以前作为关键步被知道。 | Daojin Zhou Zhao Cai Yongmin Bi Weiliang Tian Ma Luo Qian Zhang Qixian Xie Jindi Wang Yaping Li Yun Kuang Xue Duan Michal Bajdich Samira Siahrostami Xiaoming Sun | 2018 | Nano Research2018，11，3： | 6 |
| 11 | Oleic Acid and Eicosapentaenoic Acid Reverse Palmitic Acid-induced Insulin Resistance in Human HepG2 Cells via the Reactive Oxygen Species/JUN Pathway显示文摘Oleic acid(OA),a monounsaturated fatty acid(MUFA),has previously been shown to reverse saturated fatty acid palmitic acid(PA)-induced hepatic insulin resistance(IR).However,its underlying molecular mechanism is unclear.In addition,previous studies have shown that eicosapentaenoic acid(EPA),aω-3 polyunsaturated fatty acid(PUFA),reverses PA-induced muscle IR,but whether EPA plays the same role in hepatic IR and its possible mechanism involved need to be further clarified.Here,we confirmed that EPA reversed PA-induced IR in HepG2 cells and compared the proteomic changes in HepG2 cells after treatment with different free fatty acids(FFAs).A total of 234 proteins were determined to be differentially expressed after PA+OA treatment.Their functions were mainly related to responses to stress and endogenous stimuli,lipid metabolic process,and protein binding.For PA+EPA treatment,the PA-induced expression changes of 1326 proteins could be reversed by EPA,415 of which were mitochondrial proteins,with most of the functional proteins involved in oxidative phosphorylation(OXPHOS)and tricarboxylic acid(TCA)cycle.Mechanistic studies revealed that the protein encoded by JUN and reactive oxygen species(ROS)play a role in OA-and EPA-reversed PA-induced IR,respectively.EPA and OA alleviated PA-induced abnormal adenosine triphosphate(ATP)production,ROS generation,and calcium(Ca^(2+))content.Importantly,H_(2)O_(2)-activated production of ROS increased the protein expression of JUN,further resulting in IR in HepG2 cells.Taken together,we demonstrate that ROS/JUN is a common response pathway employed by HepG2 cells toward FFA-regulated IR. | Yaping Sun Jifeng Wang Xiaojing Guo Nali Zhu Lili Niu Xiang Ding Zhensheng Xie Xiulan Chen Fuquan Yang | 2021 | Genomics, Proteomics & Bioinformatics2021，19，5： | 6 |
| 12 | Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella显示文摘Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic capacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some single-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a constructed mouse na ve antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from virus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design. | CHEN CongLin SUN XiaoYun LIAO LanJie LUO ShaoXiang LI ZhouQuan ZHANG XiaoHua WANG YaPing GUO QionLin FANG Qin DAI HePing | 2013 | Science China(Life Sciences)2013，56，1： | 6 |
| 13 | Quantitative Proteomics Using Isobaric Labeling:A Practical Guide显示文摘In the past decade,relative proteomic quantification using isobaric labeling technology has developed into a key tool for comparing the expression of proteins in biological samples.Although its multiplexing capacity and flexibility make this a valuable technology for addressing various biological questions,its quantitative accuracy and precision still pose significant challenges to the reliability of its quantification results.Here,we give a detailed overview of the different kinds of isobaric mass tags and the advantages and disadvantages of the isobaric labeling method.We also discuss which precautions should be taken at each step of the isobaric labeling workflow,to obtain reliable quantification results in large-scale quantitative proteomics experiments.In the last section,we discuss the broad applications of the isobaric labeling technology in biological and clinical studies,with an emphasis on thermal proteome profiling and proteogenomics. | Xiulan Chen Yaping Sun Tingting Zhang Lian Shu Peter Roepstorff Fuquan Yang | 2021 | Genomics, Proteomics & Bioinformatics2021，19，5： | 5 |
| 14 | Time course of foreign gene integration and expression in transgenic fish embryos显示文摘Using a nuclear transplantation approach, the integration and expression of the green fluorescent protein (GFP) gene in the embryogenesis of transgenic loach (Misgurnus anguillicaudatus Cantor) have been studied. The GFP gene expression is first observed at the gastrula stage, which is consistent with the initiation of cell differentiation of fish embryos. The time course of the foreign gene expression is correlated with the regulatory sequences. The expression efficiency also depends on the gene configuration: the expression of pre-integrating circular plasmid at early embryos is higher than that of the linear plasmid. The integration of the GFP gene is first detected at the blastula stage and lasts for quite a long period. When two types of different plasmids are co-injected into fertilized eggs, the behavior of their integration and expression is not identical. | Haobin Zhao Shangping Chen Yonghua Sun Yaping Wang Zuoyan Zhu | 2000 | Chinese Science Bulletin2000，45，8： | 5 |
| 15 | Topotactic reduction of layered double hydroxides for atomically thick two-dimensional non-noble-metal alloy显示文摘Layered double hydroxides (LDHs) have been widely used as catalysts owingto their tunable structure and atomic dispersion of high-valence metal ions;however, limited tunability of electronic structure and valence states havehindered further improvement in their catalytic performance. Herein, we reducedultrathin LDH precursors in situ and topotactically converted them to atomicallythick (N2 nm) two-dimensional (2D) multi-metallic, single crystalline alloynanosheets with highly tunable metallic compositions. The as-obtained alloynanosheets not only maintained the vertically aligned ultrathin 2D structure,but also inherited the atomic dispersion of the minor metallic compositions ofthe LDH precursors, even though the atomic percentage was higher than 20%,which is far beyond the reported percentages for single-atom dispersions (usuallyless than 0.1%). Besides, surface engineering of the alloy nanosheets can finely tunethe surface electronic structure for catalytic applications. Such in situ topotacticconversion strategy has introduced a novel approach for atomically dispersedalloy nanostructures and reinforced the synthetic methodology for ultrathin 2Dmetal-based catalysts. | Pengsong Li Qixian Xie Lirong Zheng Guang Feng Yingjie Li Zhao Cai Yongmin Bi Yaping Li Yun Kuang Xiaoming Sun Xue Duan | 2017 | Nano Research2017，10，9： | 4 |
| 16 | Preliminary observation results of the Coherent Beacon Systemonboard the China Seismo-Electromagnetic Satellite-1显示文摘This paper reports, for the first time, observation results of the Coherent Beacon System(CBS) onboard the China SeismoElectromagnetic Satellite-1(CSES-1). We describe the CBS, and the Computerized Ionospheric Tomography(CIT) algorithm program is validated by numerical experiment. Two examples are shown, for daytime and nighttime respectively. The Equatorial Ionization Anomaly(EIA) can be seen, and the northern crest core is located at ~20°N in the reconstruction image at 07:28 UTC on 20 July 2018(daytime).Disturbances are shown in the reconstruction image at 18:40 UTC on 13 July 2018(nighttime). We find that beacon measurements are more consistent with ionosonde measurements than model results, by comparing Nm F2 at three sites at Lanzhou, Chongqing, and Kunming; consistency with ionosonde measurements validates beacon measurements. Finally, we have studied Vertical Total Electron Content(VTEC) variations from ground to ~500 km(the height of CSES-1 orbit) and ratios of VTEC between beacon measurements and CODE(Center for Orbit Determination in Europe) data. VTEC variation from ground to ~500 km has a range of 7.2–16.5 TECU for the daytime case and a range of 1.1–1.7 TECU for the nighttime case. The Beacon/CODE ratio of VTEC varies with latitude and time. The mean Beacon/CODE ratio is 0.69 for the daytime case and 0.26 for the nighttime case. The fact that the nighttime case yields lower ratios indicates the higher altitude of the ionosphere during nighttime when the ionosphere is assumed to be a thin layer. | Liang Chen Ming Ou YaPing Yuan Fang Sun Xiao Yu WeiMin Zhen | 2018 | Earth and Planetary Physics2018，2，6： | 3 |
| 17 | The Rice'NUTRITION RESPONSE AND ROOT GROWTH' (NRR) Gene Regulates Heading Date显示文摘Dear Editor, As one of the most important developmental traits,flowering time has been extensively investigated in the long-day plant Arabidopsis.Multiple factors are involved,including photoperiod and/or temperature (Amasino,2010).Flowering locus T (FT) is known to be an important integrator of different flowering pathways (Taoka et al.,2011).Genes of the CONSTANS (CO) family also play key roles in regulating flowering time.The CO protein consists of a zinc finger and a CCT domain shared by a group of plant-specific transcription factors that regulate photoperiodic flowering response and circadian rhythms in Arabidopsis.In the case of short-day plants such as rice,previous studies have also revealed a number of genes that control heading date,such as the CO ortholog Heading date 1 (Hdl) and,at its downstream,Heading date 3a (Hd3a),an ortholog of FT (Kojima et al.,2002;Komiya et al.,2008).Although the main photoperiodic flowering regulators are highly conserved between Arabidopsis and rice,there are genes that act differently relating to the daylength property of rice,such as Early heading datel (Ehdl),Indeterminate1 (OslD1),the MADS-box gene OsMADS51 (Doiet al.,2004;Kim et al.,2007;Park et al.,2008),and Ghd7 (Xue et al.,2008). | Yuman Zhang Ge Zhang Na Xiao Lina Wang Yaping Fu Zongxiu Sun Rongxiang Fang Xiaoying Chen | 2013 | Molecular Plant2013，6，2： | 3 |
| 18 | Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts显示文摘The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2-year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602 amino acids, including an extracellular ligand-binding domain of 244 amino acids (aa), a single transmembrane domain of 24 aa and an intracellular signal-transduction domain of 334 aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2-year common carp, a 224 bp product was identified in liver and a 321 bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 321 bp fragment could be amplified from all the tissues of 4-month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2-year common carp and 4-month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2-year common carp was significantly lower than that of 4-month common carp. | SUN Xiaofeng GUO Qionglin HU Wei WANG Yaping ZHU Zuoyan | 2006 | Progress in Natural Science:Materials International2006，16，11： | 3 |
| 19 | Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda显示文摘A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blastocyst and a giant panda specific microsatellite locus g010 was successfully amplified. DNA sequencing of the PCR product showed that two sequences of reconstructed blastocysts are the same as that of positive control giant panda. Our results prove that the nucleus of interspecies reconstructed blastocyst comes from somatic nucleus of donor giant panda. | DING Bo SHI Peng XIANGYU Jinggong ZHANG Yaping CHEN Dayuan SUN Qingyuan LI Guangpeng WANG Minkang LIU Yilong KANG Li HAN Zhiming SONG Xiangfen LI Jingsong CHEN Yuchun | 2000 | Chinese Science Bulletin2000，45，20： | 3 |
| 20 | CeO_(2)with diverse morphologies-supported IrOx nanocatalysts for efficient oxygen evolution reaction—Commemorating the 100th anniversary of the birth of Academician Guangxian Xu显示文摘IrOx-based catalysts are considered the most promising candidates for oxygen evolution reaction(OER)due to their high efficiency.However,improving their intrinsic catalytic activity is essential for practical application.In this work,CeO_(2)with three different morphologies(rod,cube,octahedron)and supported IrOx nanoparticles were fabricated,and they display morphology-dependent OER activity.The IrOx/CeO_(2)-rod shows the highest activity;the catalysts have a catalytic activity sequence of rod>cube>octahedron.A plausible mechanism was proposed:the CeO_(2)support with different morphologies modulates the electronic structure of IrOx by the synergistic interaction promoted by oxygen vacancies between the active component and the support,thereby altering the catalytic activity of the IrOx/CeO_(2)catalyst. | Qingqing Li Zhichao Zeng Xiaolei Sun Feng Luo Yaping Du | 2021 | Journal of Rare Earths2021，39，4： | 3 |
您所在的机构暂未开通此部分的功能，请联系管理员