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| 1 | CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes显示文摘染色体编辑工具例如定期聚类短 palindromic 重复(CRISPR ) 联系了的 interspaced 系统(Cas ) 广泛地被用来包括动物接合子和人的房间在模型系统修改基因,并且为基本研究和临床的应用保持巨大的诺言。迄今为止,严肃的知识差距在人的早胚胎,并且在效率和在人的培植前胚胎使用象 CRISPR/Cas9 那样的技术的潜在的离开目标效果留在我们 DNA 修理机制的理解。在这份报告,我们使用了推进的接合子调查 CRISPR/Cas9-mediated 基因在人的房间编辑的 tripronuclear (3PN ) 。我们发现 CRISPR/Cas9 能有效地劈开内长的 -globin 基因(HBB ) 。然而,相应再结合的效率指导了 HBB 的修理(HDR ) 是低的,编辑胚胎是马赛克。离开目标劈开在由 T7E1 试金并且 whole-exome 定序揭示了的这些 3PN 接合子也是明显的。而且,内长的 delta-globin 基因(HBD ) 对 HBB 相应,与外长的施主 oligos 竞争了充当修理模板,导致倔强的变化。我们的数据也显示在这些胚胎的 HBB 地点的修理通过非转线路 HDR 小径优先地发生了。一起拿,我们的工作加亮紧迫的需要进一步改进 CRISPR/Cas9 平台的忠实和特性,为编辑的 CRSIPR/Cas9-mediated 的任何临床的应用程序的一个前提。 | Puping Liang Yanwen Xu Xiya Zhang Chenhui Ding Rui Huang Zhen Zhang Jie Lv Xiaowei Xie Yuxi Chen Yujing Li Ying Sun Yaofu Bai Zhou Songyang Wenbin Ma Canquan Zhou Junjiu Huang | 2015 | Protein & Cell2015，6，5： | 149 |
| 2 | Correction of β-thalassemia mutant by base editor in human embryos显示文摘 | Puping Liang Chenhui Ding Hongwei Sun Xiaowei Xie Yanwen Xu Xiya Zhang Ying Sun Yuanyan Xiong Wenbin Ma Yongxiang Liu Yali Wang Jianpei Fang Dan Liu Zhou Songyang Canquan Zhou Junjiu Huang | 2017 | Protein & Cell2017，8，11： | 34 |
| 3 | Immunohistochemical assessment of angiogenesis in hepatocellular carcinoma and surrounding cirrhotic liver tissues显示文摘AIM: To investigate whether vascular endothelial growth factor (VEGF) was over-expressed in hepatocellular carcinoma (HCC) or in surrounding cirrhotic liver tissues.METHODS: Immunohistochemistry was performed to investigate the expression of VEGF proteins in HCC tissues from 105 consecutive patients undergoing curative resection for HCC. The immunostaining results and related clinicopathologic materials were analyzed with statistical methods. Kaplan-Meier method was used to calculate survival curves, and Log-rank test was performed to compare differences in survival rates of the patients with positive HCC staining and negative VEGF.RESULTS: VEGF-positive expression was found in 72 of105 HCC patients (68.6%). Capsular infiltration (P= 0.005),vascular invasion (P = 0.035) and intrahepatic metastasis(P=0.008) were observed more frequently in patients with VEGF-positive expression than in those with VEGFnegative expression. Kaplan-Meier curves showed that VEGF-positive expression was associated with a shorter overall survival (P = 0.014). VEGF-positive expression was found in 47 of tissues 68 HCC (69.1%), and VEGF-positive expression was found in 54 of 68 surrounding cirrhotic liver tissues (79.4%). VEGF-positive expression was significantly higher in surrounding cirrhotic liver tissues than in HCC (P= 0.017).CONCLUSION: VEGF may play an important role in the angiogenesis and prognosis of HCC, as well as in the angiogenesis of liver cirrhosis. | GeertuDeli Can-HaoJin RongMu SongYang YueLiang DeChen MasatoshiMakuuchi | 2005 | World Journal of Gastroenterology2005，11，7： | 19 |
| 4 | Effective gene editing by high-fidelity base editor 2 in mouse zygotes显示文摘通过相应再结合的指向的点 mutagenesis 广泛地在基因研究被使用了并且为在病人修理引起疾病的变化保持可观的诺言。然而,象 mosaicism 那样的问题和低 mutagenesis 效率继续提出挑战到如此的途径的临床的申请。最近,一个基础编辑器() 在 cytidine (C) 脱氨基酶上造的系统和 CRISPR/Cas9 技术在植物,酵母,和人的房间为指向的点 mutagenesis 作为一个其他的方法被开发。然而,基础编辑器高效地在 deamination 窗口中把 C 变换成 thymidine (T) 是否指向了在鼠标胚胎的基础编辑,仍然保持不清楚是可行的。在这份报告,我们产生了基础编辑的一个修改高保真版本 2 (HF2-BE2 ) ,并且调查了它在老鼠胚胎编辑功效的底。我们发现 HF2-BE2 能高效地把 C 变换成 T,与直到在老鼠胚胎的 100% biallelic 变化效率。不同于 BE3, HF2-BE2 能在目标和非目标海滨上把 C 变换成 T,扩展基础编辑器的编辑范围。令人惊讶地,我们发现 HF2-BE2 能也使脱去氨基对 gRNA 有约束力的区域近似的 C。一起拿,我们的工作表明由基础编辑,和下划线在鼠标产生点变化的可行性小心地优化编辑系统以便消除近似地点的 deamination 的底的需要。 | Puping Liang Hongwei Sun Ying Sun Xiya Zhang Xiaowei Xie Jinran Zhang zhen Zhang Yuxi Chen Chenhui Ding Yuanyan Xiong Wenbin Ma Dan Liu Junjiu Huang Zhou Songyang | 2017 | Protein & Cell2017，8，8： | 17 |
| 5 | A review of soil erodibility in water and wind erosion research显示文摘Soil erodibility is an important index to evaluate the soil sensitivity to erosion. The research on soil erodibility is a crucial tache in understanding the mechanism of soil erosion. Soil erodibility can be evaluated by measuring soil physiochemical properties, scouring experiment, simulated rainfall experiment, plot experiment and wind tunnel experiment. We can use soil erosion model and nomogram to calculate soil erodibility. Many soil erodibility indices and formulae have been put forward. Soil erodibility is a complex concept, it is influenced by many factors, such as soil properties and human activities. Several obstacles restrict the research of soil erodibility. Firstly, the research on soil erodibility is mainly focused on farmland; Secondly, soil erodibility in different areas cannot be compared sufficiently; and thirdly, the research on soil erodibility in water-wind erosion is very scarce. In the prospective research, we should improve method to measure and calculate soil erodibility, strengthen the research on the mechanism of soil erodibility, and conduct research on soil erodibility by both water and wind agents. | SONGYang LIULianyou YANPing CAOTong | 2005 | Journal of Geographical Sciences2005，15，2： | 13 |
| 6 | New Technique of Casting-rolling Strips for Semi-solid Magnesium Alloys显示文摘The conjugation of semi-solid process technique and casting-rolling technique applied to produce the magnesium stripswas studied. The semi-solid slurry has been prepared continuously by the mechanical method and its temperaturewas controlled strictly at the same time. AZ91D and AZ31 casting magnesium alloys were applied to the experiment.The casting-rolling strips with non-dendritic structure were obtained and its main mechanical property is better. Theprocess ability of the casting-rolling strips was studied. It is significative to link the semi-solid process techniques andcasting-rolling techniques, through which we can get high quality magnesium alloy strips with non-dendritic structureand improve the overall properties of the products. | Shuisheng XIE Maopeng GENG Xinmin ZHOU Ying ZHANG Songyang ZHANG Yanchun WANG Guojie HUANG | 2005 | Journal of Materials Science & Technology2005，21，6： | 9 |
| 7 | Phosphorylation of PLIN3 by AMPK promotes dispersion of lipid droplets during starvation显示文摘Dear Editor,Lipid droplets(LDs)are dynamic lipid-storage organelles of storage depots and sources of essential substrates for myriad cellular processes and protect cells from lipotoxicity(Ohsaki et al.,2006).Disrupted LD and fat storage homeostasis has been linked to metabolic diseases such as atherosclerosis,obesity,and type II diabetes(Levin et al.,2001).Structurally,the core of neutral lipids in LDs is surroun ded by a phospholipid mono layer and coated with specific proteins(Storey et al.,2011).Perilipin family of proteins are the predominant LD-associated proteins. | Jianxi Zhu Mingyang Xu Yi Liu Lisha Zhuang Kejun Ying Feng Liu Dan Liu Wenbin Ma Zhou Songyang | 2019 | Protein & Cell2019，10，5： | 7 |
| 8 | Human telomeric proteins occupy selective interstitial sites显示文摘人的 telomeres 被在六核心 telomeric 蛋白质 RAP1, TRF1, TRF2, TIN2, TPP1,和 POT1 附近装配的蛋白质建筑群绑并且保护。telomeres 上的这些蛋白质的功能广泛地被学习了。最近,增加证据在 telomeres 外面为这些蛋白质建议了可能的角色。然而,不在经典中(extra-telomeric ) 人的 telomeric 蛋白质遗体的功能糟糕理解。到这个目的,我们系统地沿着人的染色体调查了 telomeric 蛋白质的有约束力的地点,由执行为 RAP1 和 TRF2 的整个染色体的染色质 immunoprecipitation (薄片) 。(ChIP-seq ) 薄片定序表明 RAP1 和 TRF2 能在空隙的地点的一个小数字上被发现,包括对基因近似的区域。一些这些有约束力的地点包含短 telomere 重复,建议蛋白质能直接绑在空隙的地点的那 telomeric。有趣地,仅仅可得到的空隙的 telomere 的小部分包含重复的区域被 RAP1 和 TRF2 占据。宫外地表示的 TRF2 能占据另外的空隙的 telomere 重复地点,建议蛋白质集中可以支配到空隙的地点的 telomeric 蛋白质的选择指向。由 RNA 干扰减少 RAP1 和 TRF2 表示导致了指向 RAP1 、指向 TRF2 的基因的改变的抄写。我们的结果显示人的 telomeric 蛋白质能占据一小部分空隙的地点并且调整基因抄写。 | Dong Yang Yuanyan Xiong Hyeung Kim Quanyuan He Yumei Li Rui Chen Zhou Songyang | 2011 | Cell Research2011，21，7： | 7 |
| 9 | CTRP1 Attenuates UUO-induced Renal Fibrosis via AMPK/NOX4 Pathway in Mice显示文摘Clq/TNF-related protein 1(CTRP1),a conserved protein of the Clq family,plays a key role in cardiovascular and metabolic diseases.However,the role of CTRP1 in renal injury is unclear.The purpose of this study is to explore the role of CTRP1 in unilateral ureteral obstruction(UUO)-induced renal fibrosis and to elucidate the underlying mechanism.Using gene delivery system,CTRP1 was overexpressed in the kidney,then the mice were operated to induce UUO model after adenovirus transfection.It was found that the expression of CTRP1 in the renal tissue was decreased in mice after UUO.CTRP1 overexpression decreased the kidney function and kidney weight index.Moreover,CTRP1 reduced oxidative stress and renal collagen deposition in vivo.As expected,we found that CTRP1 activated AMP-activated kinase(AMPK)and decreased NOX4 expression,while silencing AMPKal abolished the protective effects of CTRP1 overexpression in mice after UUO.In conclusion,CTRP1 may protect against UUO-induced renal injury via AMPK/NOX4 signaling.Our results indicate that CTRP1 exhibits potential effects to treat renal fibrosis caused by UUO. | Wen LI Fan CHENG Yi-yan SONGYANG Song-yi-sha YANG Jie WEI Yuan RUAN | 2020 | Current Medical Science2020，40，1： | 6 |
| 10 | Questions about NgAgo显示文摘 | Shawn Burgess Linzhao Cheng Feng Gu Zhiwei Huang Shuo Lin Jinsong Li Wei Li Wei Qin Yujie Sun Zhou Songyang Wensheng Wei Qiang Wu Haoyi Wang Xiaoqun Wang Jing-Wei Xiong Jianzhong Xi Hui Yang Bin Zhou Bo Zhang Junjiu Huang | 2016 | Protein & Cell2016，7，12： | 5 |
| 11 | Working conditions of bipolar radiofrequency on human articular cartilage repair following thermal injury during arthroscopy显示文摘 | Huang Yuelong Zhang Yujun Ding Xiaoquan Liu Songyang Sun Tiezheng | 2014 | Chinese Medical Journal2014，，22： | 4 |
| 12 | Effective and precise adenine base editing n mouse zygotes显示文摘 | Puping Liang Hongwei Sun Xiya Zhang Xiaowei Xie Jinran Zhang Yaofu Bai Xueling Ouyang Shengyao Zhi Yuanyan Xiong Wenbin Ma Dan Liu Junjiu Huang Zhou Songyang | 2018 | Protein & Cell2018，9，9： | 4 |
| 13 | Live cell imaging and proteomic profiling of endogenous NEAT1 lncRNA by CRISPR/ Cas9-mediated knock-in显示文摘In mammalian cells,long noncoding RNAs(lncRNAs)form complexes with proteins to execute various biological functions such as gene transcription,RNA processing and other signaling activities.However,methods to track endogenous lncRNA dynamics in live cells and screen for lncRNA interacting proteins are limited.Here,we report the development of CERTIS(CRISPR-mediated Endogenous lncRNA Tracking and Immunoprecipitation System)to visualize and isolate endogenous lncRNA,by precisely inserting a 24-repeat MS2 tag into the distal end of lncRNA locus through the CRISPR7Cas9 technology.In this study,we show that CERTIS effectively labeled the paraspeckle lncRNA NEAT1 without disturbing its physiological properties and could monitor the endogenous expression variation of NEAT1.In addition,CERTIS displayed superior performance on both short-and long-term tracking of NEAT1 dynamics in live cells.We found that NEAT1 and paraspeckles were sensitive to topoisomerase I specific inhibitors.Moreover,RNA Immunoprecipitation(RIP)of the MS2-tagged NEAT1 lncRNA successfully revealed several new protein components of paraspeckle.Our results support CERTIS as a tool suitable to track both spatial and temporal lncRNA regulation in live cells as well as study the lncRNA-protein interactomes. | Bohong Chen Shengcheng Deng Tianyu Ge Miaoman Ye Jianping Yu Song Lin Wenbin Ma Zhou Songyang | 2020 | Protein & Cell2020，11，9： | 4 |
| 14 | Telomere regulation in pluripotent stem cells显示文摘Pluripotent 干细胞(PSC ) 有潜力从所有三基本细菌层和能力生产房间的任何类型到自我更新并且在 vitro 无止境地增殖。PSC,胚胎的干细胞(转换字符) 和导致的 pluripotent 干细胞(iPSCs ) 的二种主要类型,分享象殖民地形态学, Oct4 和 Nanog 的高表示,和强壮的碱的磷酸酶活动那样的普通特征。在最近的年里,增加证据建议那 telomere 长度在维持干细胞 pluripotency 代表另一个重要内部因素。Telomere 长度动态平衡和它的结构的正直帮助保护染色体结束免受再结合,结束熔化,和 DNA 的伤害损坏回答,保证哺乳动物的房间的师的能力。PSC 通常展出高 telomerase 活动坚持说他们的极其长、稳定的 telomeres,和新兴的数据显示小径可以玩的 telomeres (中高音) 的其他的变长在 telomere 的一个重要角色也工作。如此的特征对他们在 vivo 区分进多样的房间类型的能力多半关键。在这评论,我们将在转换字符和 iPSCs 集中于 telomeres 的功能和规定,从而使 telomere 长度的重要性清楚些到在 PSC 调整 telomeres 的 pluripotency 和机制。 | Yan Huang Puping Liang Dan Liu Junjiu Huang Zhou Songyang | 2014 | Protein & Cell2014，5，3： | 3 |
| 15 | Recent progress on gas sensors based on graphene-like 2D/2D nanocomposites显示文摘Two-dimensional(2D)nanomaterials have demonstrated great potential in the field of flexible gas sensing due to their inherent high specific surface areas,unique electronic properties and flexibility property.However,numerous challenges including sensitivity,selectivity,response time,recovery time,and stability have to be addressed before their practical application in gas detection field.Development of graphene-like 2D/2D nanocomposites as an efficient strategy to achieve high-performance 2D gas sensor has been reported recently.This review aims to discuss the latest advancements in the 2D/2D nanocomposites for gas sensors.We first elaborate the gas-sensing mechanisms and the collective benefits of 2D/2D hybridization as sensor materials.Then,we systematically present the current gas-sensing applications based on different categories of 2D/2D nanocomposites.Finally,we conclude the future prospect of 2D/2D nanocomposites in gas sensing applications. | Songyang Yuan Shaolin Zhang | 2019 | Journal of Semiconductors2019，40，11： | 3 |
| 16 | Assessing the impact of urbanization on net primary productivity using multi-scale remote sensing data: a case study of Xuzhou, China显示文摘一条改进 Carnegie Ames 斯坦福途径(房屋)基于二种遥感( RS )为数据建模, Landsat 提高了题目的 Mapper 加(ETM +)并且中等分辨率成像分光辐射函数( MODIS ),并且气候变量被使用从 2001~2010 在每年的6月估计 Xuzhou 的网络主要生产率( NPP ).当空间规模在显示出的 Xuzhou 增加了陆上的植被的平均 NPP ,学习区域的 NPP 减少了最近的年里的一个减少的趋势,多半,由于在气候和环境的变化,学习区域被划分成四个分区,是指明了在在 NPP 指定为最低分区的区域与膨胀增加了的 NPP 最高、中等高、中等低、最低的规模,显示 NPP 分发与不同植被的 NPP 打的不同空间规模变化了被规模显著地也特别地影响,因为混合象素,生产的城市的树林的 NPP 降低估计在 NPP 的类似的趋势另外与不同 RS 数据被观察,生长区域的居住区域和减小的扩大是主要原因因为在城市的区域的 NPP 变化陆地盖子变化减少了 NPP ,它能主要被归因于导致人的骚乱。 | Kun TAN Songyang ZHOU Erzhu LI Peijun DU | 2015 | Frontiers of Earth Science2015，9，2： | 2 |
| 17 | Geology, Geochronology, and Hf Isotopic Composition of the Pha Lek Fe Deposit, Northern Laos: Implications for Early Permian Subduction-Related Skarn Fe Mineralization in the Truong Son Belt显示文摘The Truong Son metallogenic belt in central Laos and Vietnam is an important Fe-Cu-Sn-Au polymetallic ore district. The Pha Lek Fe deposit is closely related to Late Carboniferous– Early Permian I-type granitic magmatism, and contains >50 Mt @ 45% to 50% of Fe ore. Ore minerals occur mainly as magnetite and hematite in the skarn alteration zone between a granitic pluton and metamorphosed Middle–Upper Devonian carbonates. The granitic pluton comprises granodiorite and granite, with zircon U-Pb dating indicating synchronous emplacement at 288.2±1.3 and 284.9±1.2 Ma, respectively. Zircons from these granitoids have ε_(Hf)(t) values of 2.9–11.2 and relatively young TDM2 ages(<1.0 Ga), indicating an origin by partial melting of depleted mafic crust or magma mixing. Previous studies have shown that these granitoids have high Y, Yb, and K_2O contents, and low Sr and Na_2O contents, which are interpreted as the melting of mafic continental crust. Pyrite of the main mineralization stage yields an ^(187)Re/^(188)Os-^(187)Os/^(188)Os isochron age of 287±17 Ma, indicating that mineralization is associated with Pha Lek granitic magmatism. A Late Carboniferous–Early Permian subduction-related skarn-type Fe mineralization model is proposed for the Pha Lek deposit. More evidence is needed to verify a hypothesis of volcanic overprinting during Late Triassic post-collisional extension. | Lin Hou Shusheng Liu Linnan Guo Fuhao Xiong Chao Li Meifeng Shi Qiming Zhang Siwei Xu Songyang Wu | 2019 | Journal of Earth Science2019，30，1： | 2 |
| 18 | The creep compaction behavior of crushed mudstones under the step loading in underground mining显示文摘The crushed rocks are used as a filling material in mined丒out areas of underground mining. Compared with the man-made filling materials, the crushed rocks exhibit higher compressibility and lower stability, which may result in instability of surrounding rock and surface subsidence. To study the creep compaction behavior of crushed mudstones, a series of creep tests are conducted. The investigations show that the creep compaction behavior of crushed mudstones is highly dependent on the original grain composition and axial stress applied on the samples. The samples with more large particles are easier to deform at initial loading stage for more large voids existed in the samples, and exhibit greater stability than those with smaller particles when the axial stress less than the bearing capacity of uframework structure When the axial stress is higher than 20 MPa, the influences of grain composition on deformability of crushed mudstones are weakened after the samples experience repeated compression. At lower stress level, the creep behavior prefers to occur in the samples w.h smaller particles, which is mainly caused by particles flow without significant particle breakage. As the axial stress increases, the single-sized sample with smaller particle size and the well-graded sample with larger Talbol power exponent n present more unstable under the constant stress. In addition, the filling of the residual intergranular voids by small particles formed by crushing and splitting behavior is the main cause of creep deformation. Lastly, a creep equation of crushed mudstones is obtained in this paper, which can agree with the experimental results in good. | Zhibiao Guo Qiong Wang Songyang Yin Xiaohui Kuai Dongshan Yan Mengyi Li Yandong Qu | 2019 | International Journal of Coal Science & Technology2019，6，3： | 2 |
| 19 | Revealing a steroid receptor ligand as a unique PPARγ 3gonist显示文摘 | Shengchen Lin Ying Han Yuzhe Shi Hui Rong Songyang Zheng Shikan Jin Shu-Yong Lin Sheng-Cai Lin Yong Li | 2012 | Cell Research2012，22，4： | 2 |
| 20 | Regulation of the order-disorder phase transition in a Cs_(2)NaFeCl_(6) double perovskite towards reversible thermochromic application显示文摘Multifunctional lead-free double perovskites demonstrate remarkable potential towards applications in various fields.Herein,an environmentally-friendly,low-cost,high-throughput Cs_(2)NaFeCl_(6) single crystal with exceedingly high thermal stability is designed and grown.It obtains a cubic lattice system in the temperature range of 80-500 K,accompanied by a completely reversible chromatic variation ranging from yellow to black.Importantly,the intriguing thermochromism is proved to own extremely high reproducibility(over 1000 cycles)without a hysteretic effect,originating from its structural flexibility that including(i)the noteworthy distortion/deformation of[NaCl_(6)]5−and[FeCl_(6)]3−octahedra;(ii)order-disorder arrangement transition of[NaCl_(6)]5−and[FeCl6]3−octahedra as the function of temperature.This study paves the way towards a new class of smart windows and camouflage coatings with an unprecedented colour range based on a Cs_(2)NaFeCl_(6) perovskite. | Wenzhe Li Naveed Ur Rahman Yeming Xian Hang Yin Yunkai Bao Yi Long Songyang Yuan Yangyi Zhang Yaxuan Yuan Jiandong Fan | 2021 | Journal of Semiconductors2021，42，7： | 2 |
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