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| 1 | Three-dimensional perfused human in vitro model of nonalcoholic fatty liver disease显示文摘AIM To develop a human in vitro model of non-alcoholic fatty liver disease(NAFLD), utilising primary hepatocytes cultured in a three-dimensional(3D) perfused platform. METHODS Fat and lean culture media were developed to directly investigate the effects of fat loading on primary hepatocytes cultured in a 3D perfused culture system. Oil Red O staining was used to measure fat loading in the hepatocytes and the consumption of free fatty acids(FFA) from culture medium was monitored. Hepatic functions, gene expression profiles and adipokine release were compared for cells cultured in fat and lean conditions. To determine if fat loading in the system could be modulated hepatocytes were treated with known anti-steatotic compounds. RESULTS Hepatocytes cultured in fat medium were found to accumulate three times more fat than lean cells and fat uptake was continuous over a 14-d culture. Fat loading of hepatocytes did not cause any hepatotoxicity and significantly increased albumin production. Numerous adipokines were expressed by fatty cells and genes associated with NAFLD and liver disease were upregulated including: Insulin-like growth factorbinding protein 1, fatty acid-binding protein 3 and CYP7A1. The metabolic activity of hepatocytes cultured in fatty conditions was found to be impaired and the activities of CYP3A4 and CYP2C9 were significantlyreduced, similar to observations made in NAFLD patients. The utility of the model for drug screening was demonstrated by measuring the effects of known antisteatotic compounds. Hepatocytes, cultured under fatty conditions and treated with metformin, had a reduced cellular fat content compared to untreated controls and consumed less FFA from cell culture medium.CONCLUSION The 3D in vitro NAFLD model recapitulates many features of clinical NAFLD and is an ideal tool for analysing the efficacy of anti-steatotic compounds. | Tomasz Kostrzewski Terri Cornforth Sophie A Snow Larissa Ouro-Gnao Cliff Rowe Emma M Large David J Hughes | 2017 | World Journal of Gastroenterology2017，23，2： | 6 |
| 2 | Nat Genet:单基因突变导致过敏性皮炎的发生显示文摘最近,研究者们鉴定出了一类导致神经性皮炎发生的关键基因突变:CARDll。来自美国NIH过敏与传染病研究所的研究者们通过对四个没有血缘关系的患病家庭进行分析,发现了这一导致疾病产生的基因、 | Chi A Ma, Yuan Zhang, Michael A Weinreich, Jonathan J Lyons, Celeste G Nelson, Thomas DiMaggio, Kelly D Stone, Joshua D Milner Jeffrey R Stinson, Elisa Ruffo, Batsukh Dorjbal, Swadhinya Arjunaraja, Kelsey Voss, Andrew L Snow Jordan K Abbott, Pia J Hauk, Paul R Reynolds, Erwin W Gelfand Elisa Ruffo Salomé Glauzy, Natsuko Yamakawa, Eric Meffre Jennifer Stoddard, Julie Niemela, Sergio D Rosenzweig Yu Zhang, Helen F Matthews Joshua J McElwee Nina Jones Alejandro Palma, Matías Oleastro, Emma Prieto, Andrea R Bernasconi, Geronimo Dubra, Silvia Danielian, Jonathan Zaiat, Marcelo A Marti Brian Kim Megan A Cooper Neil Romberg | 2017 | 现代生物医学进展2017，17，27： | 3 |
| 3 | Effect of a fall in malaria transmission on morbidity and mortality in Kilifi, Kenya显示文摘 | Wendy P O'Meara Phillip Bejon Tabitha W Mwangi Emelda A Okiro Norbert Peshu Robert W Snow Charles RJC Newton Kevin Marsh | 2008 | The Lancet2008，，9649： | 2 |
| 4 | Commercialization of transgenic plants potential ecological risks显示文摘 | SNOW A A PEDRO M P | 1997 | Bioscience1997，47，： | 1 |
| 5 | Management of venous thromboembolism: a clinical practice guideline from the American College of Physicians and the American Academy of Family Physicians显示文摘 | SNOW V QASEEM A BARRY P | 2007 | Ann Intern Med2007，146，3： | 1 |
| 6 | Occurrence of sulfonamide antimierobials in private wells in Washington County, Idaho, USA 显示文摘 | Batt A L Snow D D Aga D S | 2006 | Chemosphere2006，64，11： | 1 |
| 7 | Dependability of the non-occlusive baseline of the interupted resonance electromagnetic blood flowmeter system显示文摘 | Kolin A MacAloin RN Snow HD | 1975 | Life Sci1975，16，： | 1 |
| 8 | Towards an Optimal Network Survivability Threshold显示文摘 | Snow A Agarwal S | 2005 | IEEE Journal on Threshold Management2005，5，3： | 1 |
| 9 | Risk assessment of ge-netically engineered crops : fitness effects of virus - resistance trans-genes in wild Cucurbita pepo显示文摘 | LaughlinK D Power A G Snow A A | 2009 | Ecological Applications2009，19，5： | 1 |
| 10 | A new look at cytoskeletal NOS-1 andβ-dystroglycan changes in developing muscle and brain in control and mdx dystrophic mice显示文摘 | Janke A Upadhaya R Snow WM | | 0，，： | 1 |
| 11 | A video database of moving faces and people显示文摘 | O'TOOLE A J HARMS J SNOW S L | 2005 | IEEE Transactions on Pattern Analysis and Machine Intelligence2005，27，5： | 1 |
| 12 | Gene flow from genetically modified rice and its enviromental consequences显示文摘 | Lu B R Snow A A | 2005 | BioScience2005，55，8： | 1 |
| 13 | Arsenic and heavy metal contamination of vegetables grown in Samta village,Bangladesh显示文摘 | Alam M G Snow E T Tanaka A | 2003 | Science of the Total Environment2003，308，13： | 1 |
| 14 | Expression Kinetics of interferon and interferon-induced genes in Atlan- tic salmon ( Salmo salar ) following infection with infectious pancreatic necrosis virus and infectious salmon anaemia vi- rus 显示文摘 | Mc Beath A J Snow M Secombes C J | 2007 | Fish Shellfish Immunol2007，22，3： | 1 |
| 15 | Current diagnosis of venous thromboembolism in primary care:a clinical practice guideline from the american academy of family physicians and the american college of physicians显示文摘 | Qaseem A Snow V Barry P | 2007 | Ann Intern Med2007，146，： | 1 |
| 16 | Life-threatening rupture of a renal angiomyolipoma in a patient taking over-the- counter horse chestnut seed extract 显示文摘 | I Snow A Halpenny D Mcneill G | 2012 | J Emerg Med2012，43，6： | 1 |
| 17 | A simple and cost-effective method of DNA extraction from small formalin-fixed paraffinembedded tissue for molecular oncologic testing显示文摘 | Anthony N Snow Aaron A Stence Jonathan A Pruessner | 2014 | Carcinogenesis Clinical Pa- thology2014，30，14： | 1 |
| 18 | Interfacial conditions and bond strength in cold pressure welding by rolling显示文摘 | WRIGHT P K SNOW D A TAY C K | | 0，，01： | 1 |
| 19 | Diagnosis and treatment of low back pain:a joint clinical practice guideline from the American College of Physicians and the American Pain Society显示文摘 | Chou R Qaseem A Snow V | | 0，，03： | 1 |
| 20 | Laser pyrolysis of characterized cyanurate network polymers 显示文摘 | Walter P Snow A W | 1987 | J Appli Polym Sci1987，34，： | 1 |
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