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| 1 | A pan-cancer blueprint of the heterogeneous tumor microenvironment revealed by single-cell profiling显示文摘The stromal compartment of the tumor microenvironment consists of a heterogeneous set of tissue-resident and tumor-infiltrating cells,which are profoundly moulded by cancer cells.An outstanding question is to what extent this heterogeneity is similar between cancers affecting different organs.Here,we profile 233,591 single cells from patients with lung,colorectal,ovary and breast cancer(n=36)and construct a pan-cancer blueprint of stromal cell heterogeneity using different single-cell RNA and protein-based technologies.We identify 68 stromal cell populations,of which 46 are shared between cancer types and 22 are unique.We also characterise each population phenotypically by highlighting its marker genes,transcription factors,metabolic activities and tissue-specific expression differences.Resident cell types are characterised by substantial tissue specificity,while tumor-infiltrating cell types are largely shared across cancer types.Finally,by applying the blueprint to melanoma tumors treated with checkpoint immunotherapy and identifying a naive CD4+T-cell phenotype predictive of response to checkpoint immunotherapy,we illustrate how it can serve as a guide to interpret scRNA-seq data.In conclusion,by providing a comprehensive blueprint through an interactive web server,we generate the first panoramic view on the shared complexity of stromal cells in different cancers. | Junbin Qian Siel Olbrecht Bram Boeckx Hanne Vos Damya Laoui Emre Etlioglu Els Wauters Valentina Pomella Sara Verbandt Pieter Busschaert Ayse Bassez Amelie Franken Marlies Vanden Bempt Jieyi Xiong Birgit Weynand Yannick van Herck Asier Antoranz Francesca Maria Bosisio Bernard Thienpont Giuseppe Floris Ignace Vergote Ann Smeets Sabine Tejpar Diether Lambrechts | 2020 | Cell Research2020，30，9： | 11 |
| 2 | 1100kV/1200kV断路器大功率试验(英文)显示文摘特高压(UHV)断路器试验本身就是一项挑战,当前,KEMA已开发了用于超高压和特高压开关设备大功率测试装置。鉴于特高压断路器一般至少由双断口灭弧室组成,需要做验证其正常电流(或故障电流)开断能力的局部试验,可以只针对断路器单断口(即断路器的1/2或1/4)来进行。例如,装有均压电容的瓷柱式断路器的情形就是这样。然而,为满足对金属封闭式开关设备的试验要求(见IEC61633),在带电部件和外壳之间以及在分闸过程应具备正确的电介质应力,不做断路器整极试验在技术上是不正确的。这就要求开发新的UHV断路器试验电路。关键之处在于使用一种双级合成电路,其中一级电路放置在测试对象的附近。这种做法的好处是,测试对象的外壳可以保持地电位。各种800kV以及1100或1200kV断路器整极全面试验均证明这种方法是有效可行的。文章中展示了试验的例子。 | René Peter Paul Smeets Sander Kuivenhoven Piet Knol Adriaan Hofstee | 2009 | 南方电网技术2009，3，6： | 4 |
| 3 | Effect of process parameters on additive-free electrospinning of regenerated silk fibroin nonwovens显示文摘Silk fibroin is a biomaterial with multiple beneficial properties for use in regenerative medicine and tissue engineering.When dissolving and processing the reconstituted silk fibroin solution by electrospinning,the arrangement and size of fibers can be manifold varied and according fiber diameters reduced to the nanometer range.Such nonwovens show high porosity as well as potential biocompatibility.Usually,electrospinning of most biomaterials demands for the application of additives,which enable stable electrospinning by adjusting viscosity,and are intended to evaporate during processing or to be washed out afterwards.However,the use of such additives increases costs and has to be taken into account in terms of biological risks when used for biomedical applications.In this study,we explored the possibilities of additive-free electrospinning of pure fibroin nonwovens and tried to optimize process parameters to enable stable processing.We used natural silk derived from the mulberry silkworm Bombyx mori.After degumming,the silk fibroin was dissolved and the viscosity of the spinning solution was controlled by partial evaporation of the initial solving agent.This way,we were able to completely avoid the use of additives and manufacture nonwovens,which potentially offer higher biocompatibility and reduced immunogenicity.Temperature and relative humidity during electrospinning were systematically varied(25–35°C,25–30%RH).In a second step,the nonwovens optionally underwent methanol treatment to initiate beta-sheet formation in order to increase structural integrity and strength.Comprehensive surface analysis on the different nonwovens was performed using scanning electron microscopy and supplemented by additional mechanical testing.Cytotoxicity was evaluated using BrdU-assay,XTT-assay,LDH-assay and live-dead staining.Our findings were,that an increase of temperature and relative humidity led to unequal fiber diameters and defective nonwovens.Resistance to penetration decreased accordingly.The most uniform fiber diameters of 998±63 nm were obtained at 30°C and 25%relative humidity,also showing the highest value for resistance to penetration(0.20 N).The according pure fibroin nonwoven also showed no signs of cytotoxicity.However,while the biological response showed statistical evidence,the material characteristics showed no statistically significant correlation to changes of the ambient conditions within the investigated ranges.We suggest that further experiments should explore additional ranges for temperature and humidity and further focus on the repeatability of material properties in dependency of suitable process windows. | Alexander Kopp Ralf Smeets Martin Gosau Nadja Kröger Sandra Fuest Marius Köpf Magnus Kruse Judith Krieger Rico Rutkowski Anders Henningsen Simon Burg | 2020 | Bioactive Materials2020，5，2： | 3 |
| 4 | Diagnostic value of drain amylase for detecting intrathoracic leakage after esophagectomy显示文摘AIM:To investigate the value of elevated drain amylase concentrations for detecting anastomotic leakage(AL) after minimally invasive Ivor-Lewis esophagectomy(MIILE).METHODS:This was a retrospective analysis of prospectively collected data in two hospitals in the Netherlands. Consecutive patients undergoing MI-ILE were included. A Jackson-Pratt drain next to the dorsal side of the anastomosis and bilateral chest drains were placed at the end of the thoracoscopic procedure. Amylase levels in drain fluid were determined in all patients during at least the first four postoperative days. Contrast computed tomography scans and/or endoscopic imaging were performed in cases of a clinically suspected AL. Anastomotic leakage was defined as any sign of leakage of the esophago-gastric anastomosis on endoscopy,re-operation,radiographic investigations,post mortal examination or when gastro-intestinal contents were found in drain fluid. Receiver operator characteristic curves were used to determine the cut-off values. Sensitivity,specificity,positive predictive value,negative predictive value,risk ratio and overall test accuracy were calculated for elevated drain amylase concentrations.RESULTS:A t o t a l o f 8 9 p a t ie n t s w e re in c lu d e d between March 2013 and August 2014. No differences in group characteristics were observed between patients with and without AL,except for age. Patients with AL were older than were patients without AL(P = 0.01). One patient(1.1%) without AL died within 30 d after surgery due to pneumonia and acute respiratory distress syndrome. Anastomotic leakage that required any intervention occurred in 15 patients(16.9%). Patients with proven anastomotic leakage had higher drain amylase levels than patients without anastomotic leakage [median 384 IU/L(IQR 34-6263) vs median 37 IU/L(IQR 26-66),P = 0.003]. Optimal cut-off values on postoperative days 1,2,and 3 were 350 IU/L,200 IU/L and 160 IU/L,respectively. An elevated amylase level was found in 9 of the 15 patients with AL. Five of these 9 patients had early elevations of their amylase levels,with a median of 2 d(IQR 2-5) before signs and symptoms occurred.CONCLUSION:Measurement of drain amylase levels is an inexpensive and easy tool that may be used to screen for anastomotic leakage soon after MI-ILE. However,clinical validation of this marker is necessary. | Gijs HK Berkelmans Ewout A Kouwenhoven Boudewijn JJ Smeets Teus J Weijs Luis C Silva Corten Marc J van Det Grard AP Nieuwenhuijzen Misha DP Luyer | 2015 | World Journal of Gastroenterology2015，21，30： | 3 |
| 5 | Z-effect after intramedullary nailing systems for trochanteric femur fractures显示文摘这研究的 PurposeThe 目的是调查 Z 效果的发生在以后双落后贡献这效果的螺丝钉 intramedullary 钉系统和风险因素。我们长假设了那钉子比的由于更长的杆提供更多的颈力量突然钉并且不太多半因此是开发一个更高级的头的错误平衡压缩的力量比颈压缩 strength.MethodsIn 103 个病人起作用地对待与的这回顾的队研究一双落后螺丝钉 intramedullary 钉设备为(潜水艇) trochanteric 臀部骨折被包括。我们关于病人和手术特征分析了耐心的图表。而且,我们在 2 年的后续时期以内进行了放射线学的大小调查破裂固定的质量,为 Z 效果植入失败和预言者。重新操作风险与 multivariate 回归 analysis.ResultsThe 发生被调查(逆行) 在这研究的 Z 效果是 9%(n ?=? 80 ) ;6 从 7 Z 效果发生在短钉子组,它不是重要的。与一根长钉子被对待的病人与短钉子组比较有复杂并发症的一个重要更大的数字(中部 2 对 0.5, p ?=? 0.001 ) 。长钉子组更经常收到了红血球输血(82% 对 31% , p ? | S.J.M. Smeets G. Kuijt P.V. van Eerten | 2017 | Chinese Journal of Traumatology2017，20，6： | 2 |
| 6 | Mesenchymal stem cell-derived exosomes increase ATP levels, decrease oxidative stress and activate PI3K/Akt pathway to enhance myocardial viability and prevent adverse remodeling after myocardial ischemia/reperfusion injury显示文摘 | Fatih Arslan Ruenn Chai Lai Mirjam B. Smeets Lars Akeroyd Andre Choo Eissa N.E. Aguor Leo Timmers Harold V. van Rijen Pieter A. Doevendans Gerard Pasterkamp Sai Kiang Lim Dominique P. de Kleijn | 2013 | Stem Cell Research2013，，3： | 2 |
| 7 | Modulation of Portal Graft Inflow: A Necessity in Adult Living-Donor Liver Transplantation?显示文摘 | Roberto Troisi Guy Cammu Giuseppe Militerno Luc De Baerdemaeker Johan Decruyenaere Eric Hoste Peter Smeets Isabelle Colle Hans Van Vlierberghe Mirko Petrovic Dirk Voet Eric Mortier Uwe J. Hesse Bernard de Hemptinne | 2003 | Annals of Surgery2003，，3： | 2 |
| 8 | E selectin and intercellcular adhesion molecule-1 are released by activated human endothelial cells in vitro显示文摘 | Leeuwenberg JF Smeets EF Neefrjes JJ | 1992 | Immunology1992，77，4： | 2 |
| 9 | Ecological footprints of Benin, Bhutan, Costa Rica and the Netherlands 显示文摘 | van Vuuren D P Smeets E M W | 2000 | Ecological Economics2000，34，： | 1 |
| 10 | Endocrine metabolic respones to abdominal aortic surgery: a randomized trial of general anesthesia versus general plus epidural anesthesia 显示文摘 | Smeets H J | 1993 | World J Surg1993，17，： | 1 |
| 11 | Effects of p21 (Cipl/Wafl) at both the G1/S and the G2/M cell cycle transitions:pRb is a critical determinant in blocking DNA replication and in preventing' endoreduplication 显示文摘 | Niculescu AB Chen X Smeets M | 1998 | Mol Cell Biol1998，18，1： | 1 |
| 12 | Safety and tolerability of single intravenous doses of sugammadex administered simultaneously with rocuronium or vecuronium in healthy volunteers 显示文摘 | Cammu G De Kam PJ Demeyer I Decoopman M Peeters PA Smeets JM | 2008 | Br J Anaesth2008，100，3： | 1 |
| 13 | A randomized con-trolled trial with an anti-CCL2 ( anti-monocyte chemotactic pro-tein 1 ) monoclonal antibody in patients with rheumatoidarthritis显示文摘 | Haringman JJ Gerlag DM Smeets TJ | 2006 | Arthritis Rheum2006，54，8： | 1 |
| 14 | Analysis of the cell infiltrate and expression of proinflammatory cytokines and matrix metalloprotei- nases in arthroscopic synovial biopsies: comparison with synovial samples from patients with end stage, destructive rheumatoid arthritis 显示文摘 | Smeets T J Barg E C Kraan M C | 2003 | Ann Rheum Dis2003，62，7： | 1 |
| 15 | Biomarkers of satiation and satiety 显示文摘 | DE G C BLOM W A SMEETS P A | 2004 | Am J Clin Nutr2004，79，6： | 1 |
| 16 | Reduction of pain catastrophizing mediates the outcome of both physical and cognitive-behavioral treatment inchronic low back pain 显示文摘 | Smeets R J Vlaeyen JW Kester AD | 2006 | J Pain2006，17，： | 1 |
| 17 | Direct NO and N2O decomposition and NO-assisted N2O decomposition over Cu-zeolites: Elucidating the influence of the Cu-Cu distance on oxygen migration 显示文摘 | Smeets P J Groothaert M H van Teeffelen R M | 2007 | J Catal2007，245，： | 1 |
| 18 | Synthesis,X-ray crystal structure and magenetism of (C2H5OH)2-a second example of a ferromagnetically coupled five-coordinated high spin dichloro-bridged nicked(Ⅱ)dimmer显示文摘 | Kolnaar J J A Smeets W J J | 1998 | Eur J Inorg Chem1998，，： | 1 |
| 19 | Toll-like receptor 2 pathway drives streptococcal cell wall-induced joint inflammation: critical role of myeloid differentiation factor 88 显示文摘 | Joosten LA Koenders MI Smeets RL | 2003 | J Immunol2003，171，11： | 1 |
| 20 | Analysis of differences in growth of cucumber genotypes under low light conditions in relation to night temperature显示文摘 | Nijs A P Smeets L | 1987 | Euphytica1987，36，1： | 1 |
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