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| 1 | Efficiency of HAART in the prevention of mother to children HIV-1 transmission at Saint Camille medical centre in Burkina Faso,West Africa显示文摘Objective:To evaluate efficiency of HAART in the prevention of mother to child HIV transmission.Methods:A longitudinal study was conducted on 1 300 women attending the antenatal service at Saint Camille Medical Centre from September 2010 to July 2011.The HIV status of mothers was determined by rapid tests and EUSA.Discordant results were confirmed by real-time PCR.PCR was used to determine HIV status of children born from HIV-positive mothers.Results:Among 1 300 pregnant women tested for HIV,378 were seropositive.Mothers were predominantly housewives(69.7%),and their mean age was(28.32±0.15) years.The overall prevalence of HIV transmission from mother to child was 4.8%(18/378).This prevalence differed significantly from 0.0%(0/114) to 6.8%(18/264) in children born from mothers under HAART and those with mothers under New Prophylactic Protocol(ACT + 3TC + NVP),respectively(P< 0.01).Children’s mortality rate during the medical follow up was 1.3%(5/378).Among 16 women with HIV dubious status by ELISA,the Real Time PCR confirmed 2/16(12.5%) as HIV positive. Conclusions:The protocol of prevention of mother to children HIV transmission(PMTCT) is effective.The rate of HIV vertical transmission is significantly reduced.Early diagnosis determined by PCR of children born from HIV- positive mother is necessary and recommended in the context of PMTCT in Burkina Faso.We also found that PCR is an effective tool to confirm HIV status in pregnant women. | Laure Stella Ghoma Linguissi Cyrille Bisseye Tani Sagna Bolni Marius Nagalo Djeneba Ouermi Florencia W Djigma Salvatore Pignatelli Joseph D Sia Virginio Pietra Remy Moret Jean Baptiste Nikiema Jacques Simpore | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，12： | 4 |
| 2 | Antimicrobial resistance of abnormal vaginal discharges microorganisms in Ouagadougou,Burkina Faso显示文摘Objective:To assess the prevalence of bacterial strains and fungal strains infecting the vaginal tract and test their sensitivity to antibiotics in women attending Saint Camille Medical Centre in Ouagadougou.Methods:From January 2008 to December 2009,a total of 2000 vaginal swabs were cultivated for bacterial and fungal identification and isolation.Furthermore,bacterial strains were tested for their susceptibility to several antibiotics used in routine in the centre. Results:The results revealed that microbial isolation and identification was attempted for 1536/2000 sample,a positivity rate of 76.80%.Candida albicans(48.76%),followed by Escherichia coli(16.67%),Streptococcus agalactiae(8-14%) and Staphylococcus aureus(7.55%) were the major agents of genital tract infections in patients.Mycoplasma hominis and Ureaplasma urealyticum combined accounted for less than 7%.Trichomonas vaginalis was identified in 1.04%coses.The antimicrobial tests revealed that the microorganisms developed resistance to several antibiotics including beta lactams.However,antibiotics such as cefamenzol,ciprofloxacin and norfloxacin were still active on these bacteria.Conclusions:The results reveal that many sexually active women are infected by one or more microbial pathogens,probably because of the lock of hygiene or the adoption of some risky behaviors,such as not using condoms or having multiple sexual partners.Efforts should be made to address these points in the country. | Simplice D Karou Florencia Djigma Tani Sagna Christelle Nadembega Moctar Zeba Aboudoulaye Kabre Kokou Anani Djeneba Ouermi Charlemagne Gnoula Virginio Pietra Salvatore Pignatelli Jacques Simpore | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，4： | 2 |
| 3 | Seroepidemiology of Hepatitis B and C Viruses in the General Population of Burkina Faso显示文摘 | Issoufou Tao Tegwindé R. Compaoré Birama Diarra Florencia Djigma Theodora M. Zohoncon Maléki Assih Djeneba Ouermi Virginio Pietra Simplice D. Karou Jacques Simpore Annarosa Floreani | 2014 | Hepatitis Research and Treatment2014，，： | 1 |
| 4 | In vitro antiplasmodial activity of crude extracts from Togolese medicinal plants显示文摘Objective:To investigate the antimalarial effect of a few plants in Togo folk medicine. Methods:After ethnobotanical survey,Opilia celtidifolia,Pavetta corymbosa(P.corymbosa) and Tamarindus indica(T.indica) were selected for screening.In vitro antimalarial tests were performed on crude extracts against fresh clinical isolates of Plasmodium falciparum using the semi microtest.Results:Different IC50,values of the extracts ranged from 2.042 to 100.000μg/mL. According to the results,the methanol extract of aerial part of P.corymbosa followed by aqueous extract of fruit of T.indica were the most active(IC50 of 2.042 and 4.786μg/mL,respectively). Qualitative test revealed the presence of alkaloids in the leaves of P.corymbosa that may be responsible for the activity of the plant.Conclusions:Our study provides scientific evidence for usage of plant in the folk medicine,and further studies are needed for identification and purification of the active principles. | Koffi Koudouvo Simplice D.Karou Denise P.Ilboudo Kouami Kokou Kodjo Essien Kodjo Aklikokou Comlan de Souza Jacques Simpore Mensavi Gbeassor | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，2： | 1 |
| 5 | Antimicrobial,antiplasmodial,haemolytic and antioxidant activities of crude extracts from three selected Togolese medicinal plants显示文摘Objective:To investigate the antioxidant,antimicrobial,antiplasmodial,acute toxicity and haemolytic activities of methanolic extracts of three plants.Phytochemical analysis to determine the phenolic contents was also carried out.Methods:The 2,2-diphenyl-1- picryl-hydrazyl(DPPH) free radical scavenging,NCCLS broth microdilution and Plasmodium Lactate Dehydrogenase(pLDH) assays were used to determine antioxidant,antimicrobial and antiplasmodial activities,respectively.Haemolysis assay was conducted on A+ human red blood cells and acute toxicity on male Swiss albino mice.Phenolics were quantitatively determined using spectrophotometric methods.Results:The DPPH assay yielded interesting antioxidant activities of methanolic extract of Parinari curatellifolia(P.curatellifolia) and Entada africana (E.africana)(IC50 were 0.20±0.01μg/mL and 0.47±0.01μg/mL,respectively).This activity was highly correlated with phenolic contents of extracts.The antimicrobial tests displayed minimal inhibitory concentrations(MICs) values ranging from 0.90 to 1.80 mg/mL for Serratia marcescens (5.marcescens) the most susceptible bacterial strain.MIC value was 1.20 mg/mL for susceptible fungal strains including Mucor rouxi(M.rouxi),Fusarium oxyporum(F.oxyporum) and Rhizopus nigricans(R.nigricans).pLDH assay showed moderate antiplasmodial activity of Balanites aegyptiaca(B.aegyptiaca)(IC50= 24.56±3.45μg/mL),however this extract was highly haemolytic and toxic in mice(LD50= 625±128mg/kg).Conclusions:Our results support in part the use of the selected plants in the treatment of microbial infections.In addition the plant showed interesting antioxidant activity that could be useful in the management of oxidative stress. | Simplice D Karou Tchadjobo Tchacondo Lassina Ouattara Kokou Anani Aly Savadogo Amegnona Agbonon Mossaclok Ben Attaia Comlan de Souza Mohsen Sakly Jacques Simpore | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，10： | 1 |
| 6 | Epidemiology and antibiotic resistance of bacterial meningitis in Dapaong,northern Togo显示文摘Objective:To assess the seasonality of the bacterial meningitis and the antibiotic resistance of incriminated bacteria over the last three years in the northern Togo.Methods:From January 2007 to January 2010,533 cerebrospinal fluids(CSF) samples were collected from patients suspected of meningitis in the Regional Hospital of Dapaong(northern Togo).After microscopic examination,samples were cultured for bacterial identification and antibiotic susceptibility. Results:The study included 533 patients(306 male and 227 female) aged from 1 day to 55 years [average age(13.00±2.07) years].Bacterial isolation and identification were attempted for 254/533 (47.65%) samples.The bacteria]species identified were:Neisseria meningitidis A(N.meningitidis A)(58.27%),Neisseria meningitidis W135(N,meningitidis W135)(7.09%),Streptococcus pneumoniae(S.pneumoniae)(26.77%),Haemophilus influenza B(H.influenza B)(6.30%) and Enterobacteriaceae(1.57%).The results indicated that bacterial meningitis occur from November to May with a peak in February for H.influenzae and S.pneumoniae and March for Neisseriaceae. The distribution of positive CSF with regards to the age showed that subjects between 6 and 12 years followed by subjects of 0 to 5 years were most affected with respective frequencies of 67.82%and 56.52%(P<0.001).Susceptibility tests revealed that bacteria have developed resistance to several antibiotics including aminosides(resistance rate>20%for both bacterial strains),macrolides(resistance rate>30%for H.influenzae) quinolones(resistance rate>15% for H.influenzae and N.meningitidis W135).Over three years,the prevalence of S.pneumoniae significantly increased from 8.48%to 73.33%(P<0.001),while the changes in the prevalence of H.influenzae B were not statistically significant:4.24%,vs.8.89%,(P= 0.233).Conclusions: Our results indicate that data in African countries differ depending on geographical location in relation to the African meningitis belt.This underlines the importance of epidemiological surveillance of bacterial meningitis. | Simplice D Karou Abago Balaka Mitiname Bamok Damhan Tchelougou Malki Assih Kokou Anani Kodjo Agbonoko Jacques Simpore Comlan de Souza | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，11： | 1 |
| 7 | Epidemiology of bacterial resistance in gastro-intestinal pathogens in a tropical area显示文摘 | Giovanni Bonfiglio Jacques Simporè Salvatore Pignatelli Salvatore Musumeci Maria Letizia Solinas | 2002 | International Journal of Antimicrobial Agents2002，，5： | 1 |
| 8 | Enteric parasites prevalence at Saint Camille Medical Centre in Ouagadougou,Burkina Faso显示文摘Objective:To assess the prevalence of parasitic infections among patients attending Saint Camille Medical Centre and to estimate co-parasitic infections rates.Methods:From January to December 2009,stool samples were collected from 11 728 persons,aged from five months to 72 years and suffering from gastroenteritis.After macroscopic description,the stools were examined by light microscopy to search for the presence of parasites.Results:From the 11 728 analyzed stools,6 154(52.47%) were infected with at least one parasite.Protozoan frequently encountered were:Giardia intestinalis(43.47%),Entamoeba histolytica/Entamoeba dispar(30.74%) and Trichomonas irUestinalis(21.72%),while Hymenolepis nana(2.25%) was the most common helminth.Co-infections occurred in 22.34%cases.Within the multi-infected samples,dual and triple infections accounted for 71.18%and 20.00%,respectively.Giardia irUestinalis for protozoan and Hymenolepis nana for helminths were the most implicated co-infections.Conclusions: This study confirms that intestinal parasites are still a public health problem in Burkina Faso.To reduce the incidence of parasitic infections,it is necessary to promote the education of people so that they practice the rules of individual and collective hygiene. | Simplice Damintoti Karou Drissa Sanou Djeneba Ouermi Salvatore Pignatelli Virginio Pietra Remy Moret Jean Baptiste Nikiema Jacques Simpore | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，5： | 1 |
| 9 | Glucose-6-phosphate dehydrogenase(G6PD) deficiency is associated with asymptomatic malaria in a rural community in Burkina Faso显示文摘Objective:To investigate 4 combinations of mutations responsible for glucose-6—phosphate dehydrogenase(G6PD) deficiency in a rural community of Burkina Faso,a malaria endemic country.Methods:Two hundred individuals in a rural community were genotyped for the mutations A376 G.G202A,A542 T,G680T and T968 C using TaqMan single nucleotide polymorphism assays and polymerase chain reaction followed by restriction fragment length polymorphism.Results:The prevalence of the G6 PD deficiency was 9.5%,in the study population.It was significantly higher in men compared to women(14.23%vs 6.0%,P=0.049).The 202A/376 G G6PD Awas the only deficient variant detected.Plasmodium falciparum asymptomatic parasitemia was significantly higher among the C6PD-non—deficient persons compared to the G6PD-deficient(P<0.001).The asymptomatic parasitemia was also significantly higher among G(SPI) nondeficient compared to C6PD—heterozygous females(P<0.001).Conclusions:This study showed that the G6 PD A- variant associated with protection against asymptomatic malaria in Burkina Faso is probably the most common deficient variant. | Abdoul Karim Ouattara Cyrille Bisseye Bapio Valery Jean Télesphore Elvira Bazie Birama Diarra Tegwindé Rebeca Compaore Florencia Djigma Virginio Pietra Remy Moret Jacques Simpore | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，8： | 0 |
| 10 | Digestive fungal flora in asymptomatic subjects in Bobo-Dioulasso,Burkina Faso显示文摘Objective::To identify Candida species in asymptomatic subjects in Bobo-Dioulasso(Burkina Faso) by the matrix—assisted laser desortption ionization—time of flight mass spectrometry.Methods:A cross-sectional study was conducted from January to February 2013 in BohoDioulasso to coiled fecal and urine specimens from voluntary donors.Fungal strains were isolated on Sabouraud dextrose agar and analyzed using matrix—assisted laser desorption ionisation—time-of—flight mass spectrometry.Results:A total of 135 samples including stools(78.5%,106/135) and urine(21.5%,29/135) were analyzed.The results revealed that fecal specimens contained mainly Candida krusei(C.krusei)(42.5%) followed by Candida albicans(29.3%),Candida glabrata(18.0%) and Candida tropicalis(C.tropicalis)(4.7%).C.krusei(34.6%) was also found to be the most frequently identified in urine samples followed by Candida albicans(27.0%).C.tropicalis(15.4%) and Candida parapsilosis,However,uncommon species such as Candida nivariensis.Candida kefyr.Candida norvegensis,Candida parapsilosis.Candida lusitaniae and Candida robusta were also identified from fecal and urines samples.Conclusions:This study noted the emergence of species such as C.krusei,Candida glabrata,Candida parapsiolosis,C.tropicalis,Candida nivariensis,Candida norvegensis,and others.It is an imperative to take into account the existence of these species in the therapeutic management of patients in Bobo—Dioulasso. | Bamba Sanata Ouédraogo Abdoul Salam Sangaré Ibrahim Zida Adama Cissé Mamoudou Karou D.Simplice Simpore Jacques Guiguemdé T.Robert Hennequin Christophe | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，8： | 0 |
| 11 | Seroprevalence of toxoplasmosis and rubella in pregnant women attending antenatal private clinic at Ouagadougou,Burkina Faso显示文摘Objective:To evaluate the prevalence of toxoplasmosis and rubella among pregnant women at Ouagadougou in Burkina Faso.Methods:All patient sera were tested for rubella and toxoplasmosis anti-IgG using commercial ELISA kits(PlateliaTMRubella IgG and Platelia? Toxo IgG).The presence of anti-rubella and anti-toxoplasmosis tgM in serum samples was tested using commercial ELISA kits Platelia Rubella TgM and Platelia Toxo IgM.Results:Among all the pregnant women tested for toxoplasmosis and rubella,their prevalence were 20.3% and 77.0%,respectively.Pregnant women in the age group of 18-25 years showed the highest frequency of anti-toxoplasmosis(34.5%) and anti-rubella IgG(84.6%).The prevalence of anti-toxoplasma and anti-rubella IgG decreased between 2006 and 2008 from 32.7%to 12.1%and 84.6% to 65.0%,respectively.There was no significant association between age and the mean titer of anti-toxoplasmosis IgG among pregnant women.Conclusions:The diagnosis of toxoplasmosis and rubella is necessary in pregnant women in Burkina Faso because of the low immunization coverage rate of rubella and the high level of exposure to these two infections which can be harmful to the newborn if contracted by women before the third trimester of pregnancy. | Laure Stella Ghoma Linguissi Bolni Marius Nagalo Cyrille Bisseye Thrse S Kagon Mahamoudou Sanou Issoufou Tao Victoire Benao Jacques Simpor Bibiane Kon | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，10： | 0 |
| 12 | Genetic diversity of hepatitis viruses in West-African countries from 1996 to 2018显示文摘The severity of hepatic pathology and the response to treatment depend on the hepatitis virus genotype in the infected host. The objective of this review was to determine the distribution of hepatitis virus genotypes in West African countries. A systematic review of the literature in PubMed, Google Scholar and Science Direct was performed to identify 52 relevant articles reporting hepatitis A, B, C, D, E and G viruses genotypes.Hepatitis B virus(HBV) genotype E with a prevalence of 90.6%(95%CI: 0.891-0.920) found in this review, is characterized by low genetic diversity. Hepatitis C virus(HCV) genotypes 1 and 2 represented 96.4% of HCV infections in West African countries, while hepatitis delta virus, hepatitis A virus, hepatitis G virus genotypes 1 and HEV genotype 3 were reported in some studies in Ghana and Nigeria. HBV genotype E is characterized by high prevalence, low genetic diversity and wide geographical distribution. Further studies on the clinical implications of HBV genotype E and HCV genotypes 1 and 2 are needed for the development of an effective treatment against this viral hepatitis in West African countries. Surveillance of the distribution of different genotypes is also needed to reduce recombination rates and prevent the emergence of more virulent viral strains. | Maléki Assih Abdoul Karim Ouattara Birama Diarra Albert Theophane Yonli Tegwindé Rebeca Compaore Dorcas Obiri-Yeboah Florencia Wendkuuni Djigma Simplice Karou Jacques Simpore | 2018 | World Journal of Hepatology2018，10，11： | 0 |
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