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| 1 | Influence of UAV flight speed on droplet deposition characteristics with the application of infrared thermal imaging显示文摘A plant protection unmanned aerial vehicle(UAV)applied for spraying pesticide has the advantages of low cost,high efficiency and environmental protection.However,the complex and changeable farmland environment is not conductive to perform spray test effectively.It is therefore necessary to carry out spray test under controlled conditions.The current study aimed to illuminate the variation law of droplet deposition characteristics under different UAV flight speeds,and to verify the feasibility for applying infrared thermal imaging in detection of droplet deposition effects.A UAV simulation platform with an airborne spray system was established and an analysis program Droplet Analysis for dealing with water-sensitive paper was developed.The results showed that,when the flight speed was set at 0.3 m/s,0.5 m/s,0.7 m/s,0.9 m/s and 1 m/s,respectively,the droplet deposition density,droplet deposition coverage and arithmetic mean of droplet size D0 decreased as the UAV flight speed increased.On the contrary,the droplet diameter variation coefficient CV increased with the increase of UAV flight speed,resulting in the worse uniformity of sprayed droplet distribution as well.The results can provide a theoretical support for optimizing the spraying parameters of plant protection UAV,and demonstrate the practicability of infrared thermal imaging in evaluating the droplet deposition in the field of aerial spraying. | Meiqiao Lv Shupei Xiao Yu Tang Yong He | 2019 | International Journal of Agricultural and Biological Engineering2019，12，3： | 9 |
| 2 | The histone methyltransferase EZH2 primes the early differentiation of follicular helper T cells during acute viral infection显示文摘Epigenetic modifications to histones dictate the differentiation of naïve CD4^(+) T cells into different subsets of effector T helper(TH)cells.The histone methyltransferase enhancer of zeste homolog 2(EZH2)has been implicated in the mechanism regulating the differentiation of TH1,TH2 and regulatory T(Treg)cells.However,whether and how EZH2 regulates follicular helper T(TFH)cell differentiation remain unknown.Using a mouse model of acute lymphocytic choriomeningitis virus(LCMV)infection,we observed abundant EZH2 expression and associated H3K27me3 modifications preferentially in the early committed virus-specific TFH cells compared to those in TH1 cells.Ablation of EZH2 in LCMV-specific CD4^(+) T cells leads to a selective impairment of early TFH cell fate commitment,but not late TFH differentiation or memory TFH maintenance.Mechanistically,EZH2 specifically stabilizes the chromatin accessibility of a cluster of genes that are important for TFH fate commitment,particularly B cell lymphoma 6(Bcl6),and thus directs TFH cell commitment.Therefore,we identified the chromatin-modifying enzyme EZH2 as a novel regulator of early TFH differentiation during acute viral infection. | Xiangyu Chen Guoshuai Cao Jialin Wu Xinxin Wang Zhiwei Pan Jianbao Gao Qin Tian Lifan Xu Zhirong Li Yaxing Hao Qizhao Huang Pengcheng Wang Minglu Xiao Luoyingzi Xie Shupei Tang Zhenyu Liu Li Hu Jianfang Tang Ran He Li Wang Xinyuan Zhou Yuzhang Wu Mengjie Chen Beicheng Sun Bo Zhu Jun Huang Lilin Ye | 2020 | Cellular & Molecular Immunology2020，17，3： | 4 |
| 3 | FGFR/RACK1 interacts with MDM2, promotes P53 degradation, and inhibits cell senescence in lung squamous cell carcinoma显示文摘Objective:FGFR is considered an important driver gene of lung squamous cell carcinoma(LSCC).Thus,identification of the biological events downstream of FGFR is important for the treatment of this malignancy.Our previous study has shown that the FGFR/RACK1 complex interacts with PKM2 and consequently promotes glycolysis in LSCC cells.However,the biological functions of the FGFR/RACK1 complex remain poorly understood.Methods:Anchorage-independent assays and in vivo tumorigenesis assays were performed to evaluate cancer cell malignancy.Distant seeding assays were performed to evaluate cancer cell metastasis.β-gal staining was used to examine cell senescence,and immunoprecipitation assays were performed to examine the interactions among FGFR,RACK1,and MDM2.Results:FGFR/RACK1 was found to regulate the senescence of LSCC cells.Treatment with PD166866,an inhibitor of FGFR,or knockdown of RACK1 induced senescence in LSCC cells(P<0.01).A molecular mechanistic study showed that FGFR/RACK1/MDM2 form a complex that promotes the degradation of p53 and thus inhibits cell senescence.PD166866 and RG7112,an MDM2/p53 inhibitor,cooperatively inhibited the colony formation and distal seeding of LSCC cells(P<0.01),and upregulated the expression of p53 and p21.Conclusions:Together,our findings revealed the regulatory roles and mechanisms of FGFR/RACK1 in cell senescence.This understanding should be important in the treatment of LSCC. | Tao Chen Fei Wang Shupei Wei Yingjie Nie Xiaotao Zheng Yu Deng Xubin Zhu Yuezhen Deng Nanshan Zhong Chengzhi Zhou | 2021 | Cancer Biology & Medicine2021，18，3： | 3 |
| 4 | Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq显示文摘B cell response plays a critical role against SARS-CoV-2 infection.However,little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection.Here,we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients,and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses.Via linking BCR to antigen specificity through sequencing(LIBRA-seq),we identified a distinct activated memory B cell subgroup(CD11c^(high) CD95^(high))had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells.Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection.The public antibody clonotypes were shared by distinct convalescent individuals.Moreover,several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain(RBD)or nucleoprotein(NP)via ELISA assay.Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro.Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level. | Bing He Shuning Liu Yuanyuan Wang Mengxin Xu Wei Cai Jia Liu Wendi Bai Shupei Ye Yong Ma Hengrui Hu Huicui Meng Tao Sun Yanling Li Huanle Luo Mang Shi Xiangjun Du Wenjing Zhao Shoudeng Chen Jingyi Yang Haipeng Zhu Yusheng Jie Yuedong Yang Deyin Guo Qiao Wang Yuwen Liu Huimin Yan Manli Wang Yao-Qing Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 5 | Chromosome doubling mediates superior drought tolerance in Lycium ruthenicum via abscisic acid signaling显示文摘Plants are continuously affected by unfavorable external stimuli,which influences their productivity and growth.Differences in gene composition and expression patterns lead homologous polyploid plants to exhibit different physiological phenomena,among which enhanced environmental adaptability is a powerful phenotype conferred by polyploidization.The mechanisms underlying the differences in stress tolerance between diploids and autotetraploids at the molecular level remain unclear.In this research,a full-length transcription profile obtained via the singlemolecule real-time(SMRT)sequencing of high-quality single RNA molecules for use as background was combined with next-generation transcriptome and proteome technologies to probe the variation in the molecular mechanisms of autotetraploids.Tetraploids exhibited an increase in ABA content of 78.4%under natural conditions and a superior stress-resistance phenotype under severe drought stress compared with diploids.The substantial differences in the transcriptome profiles observed between diploids and autotetraploids under normal growth conditions were mainly related to ABA biosynthesis and signal transduction pathways,and 9-cis-epoxycarotenoid dioxygenase 1(NCED1)and NCED2,which encode key synthetic enzymes,were significantly upregulated.The increased expression of the ABREbinding factor 5-like(ABF5-like)gene was a pivotal factor in promoting the activation of the ABA signaling pathway and downstream target genes.In addition,ABA strongly induced the expression of osmotic proteins to increase the stress tolerance of the plants at the translational level.We consider the intrinsic mechanisms by which ABA affects drought resistance in tetraploids and diploids to understand the physiological and molecular mechanisms that enhance abiotic stress tolerance in polyploid plants. | Shupei Rao Yuru Tian Xinli Xia Yue Li Jinhuan Chen | 2020 | Horticulture Research2020，7，1： | 2 |
| 6 | The coloring mechanism of a novel golden variety in Populus deltoides based on the RGB color mode显示文摘Compared to flower color and red leaf mutants,the mechanism of yellow leaf formation in woody plants is more complicated and less understood.Poplars are common and widely distributed perennial trees.Here,we obtained a golden leaf mutant poplar variety(JHY)and compared it with its original green leaf poplar(Populus sp.Linn.‘2025’)in terms of phenotype,pigment content,the transcriptomes and metabolomes.Through transcriptome sequencing,we found that the chlorophyll degradation genes,and the genes in anthocyanin metabolism in JHY were up-regulated compared to L2025 and the carotenoid synthesis genes were down-regulated.Further based on HPLC-ESI-MS/MS technology,16 differentially expressed anthocyanins were identified,14 reddish anthocyanins of which were significantly up-regulated.According to these results,we proposed a coloring mechanism of JHY based on the RGB color mode.The yellow color of carotenoids and the red color of anthocyanins overlay each other,combined with a reduction in chlorophyll,turning the leaves golden.This study systematic analyzes the causes of golden leaf formation through the omics integration approach,which will provide reference for the breeding of golden leaf trees. | Yuru Tian Shupei Rao Qianqian Li Meng Xu Aike Wang Hechen Zhang Jinhuan Chen | 2021 | Forestry Research2021，1，1： | 2 |
| 7 | Plant lncRNAs are enriched in and move systemically through the phloem in response to phosphate deficiency显示文摘In response to phosphate(Pi) deficiency, it has been shown that micro-RNAs(miRNAs) and mRNAs are transported through the phloem for delivery to sink tissues. Growing evidence also indicates that long noncoding RNAs(lncRNAs) are critical regulators of Pi homeostasis in plants. However, whether lncRNAs are present in and move through the phloem, in response to Pi deficiency, remains to be established. Here, using cucumber as a model plant, we show that lncRNAs are enriched in the phloem translocation stream and respond,systemically, to an imposed Pi-stress. A well-known lncRNA, IPS1, the target mimic(TM) of miRNA399,accumulates to a high level in the phloem, but is not responsive to early Pi deficiency. An additional 24 miRNA TMs were also detected in the phloem translocation stream; among them miRNA171 TMs and miR166 TMs were induced in response to an imposed Pi stress.Grafting studies identified 22 lncRNAs which move systemically into developing leaves and root tips. A CU-rich PTB motif was further identified in these mobile lncRNAs. Our findings revealed that lncRNAs respond to Pi deficiency, non-cell-autonomously, and may act as systemic signaling agents to coordinate early Pi deficiency signaling, at the whole-plant level. | Zhaoliang Zhang Yi Zheng Byung-Kook Ham Shupei Zhang Zhangjun Fei William J.Lucas | 2019 | Journal of Integrative Plant Biology2019，61，4： | 2 |
| 8 | Aerodynamic Characteristics of Isolated Loaded Tires with Different Tread Patterns: Experiment and Simulation显示文摘The current research of tire aerodynamics mainly focus on the isolated and simplified tread tire.Compared with the real complex pattern tire,the tread pattern structure and deformed profile of a loaded tire has a greatly influence on tire aerodynamic drag.However,the mechanisms of the isolated loaded tires with different tread patterns effects on the aerodynamic drag are subjects worthy of discussion.The purpose of this study is to experimentally and compu-tationally investigate the aerodynamic characteristics of three tires 185/65 R14 with different patterns under loaded.A wind tunnel test model was first established using three-dimensional(3D)printing with a ratio of 1:1,and the pres-sure coefficients C_(p) of the three tires with different patterns are measured.The paper then conducted computational fluid dynamics(CFD)simulations for analyzing the pressure and flow characteristics.The accuracy of CFD simulation is verified by comparing the simulation results with the test results of pressure coefficients C_(p),and they are of good consistency.While,the general analysis of pressure coefficients C_(p) results of the three tires indicates high-pressure area on the windward surface,and occurrence of low-pressure area on the leeward surface,the pressure coefficients C_(p) of all three tires decreased firstly and then increased along in the air flow direction.The authors finally analyzed the effect of tread patterns on the flow field around the tire and revealed the differences between flow characteristics and aerodynamic drag.The results show that,angle of tire lateral groove has great effect on the flow field characteristics such that;the more the angle of lateral groove agrees with the air flow direction,the less the flow separation and flow vortices,and a minimum observable aerodynamic drag.The research provides a guidance for the design of low aerodynamic drag tires,and helps to illustrate the impact of tire aerodynamics on the car body in the future. | Haichao Zhou Zhen Jiang Guolin Wang Shupei Zhang | 2021 | Chinese Journal of Mechanical Engineering2021，34，2： | 2 |
| 9 | Hormonesensitive lipase knockout mice have increased hep- atic insulin sensitivity and are protected from shortterm diet-induced insulin resistance in skeletal muscle and heart显示文摘 | PARK S Y KIM H J SHUPEI W | 2005 | American Journal of Physiology- Endocrinology and Metabolism2005，289，8： | 1 |
| 10 | Controlled synthesis of Mn 3 O 4 nanocrystallites and MnOOH nanorods by a solvothermal method显示文摘 | Weixin Zhang Zeheng Yang Yi Liu Shupei Tang Xiaozhao Han Min Chen | 2004 | Journal of Crystal Growth2004，，1： | 1 |
| 11 | Porous Co_(2)VO_(4) Nanodisk as a High-Energy and Fast-Charging Anode for Lithium-Ion Batteries显示文摘High-energy–density lithium-ion batteries(LIBs)that can be safely fast-charged are desirable for electric vehicles.However,sub-optimal lithiation potential and low capacity of commonly used LIBs anode cause safety issues and low energy density.Here we hypothesize that a cobalt vanadate oxide,Co_(2)VO_(4),can be attractive anode material for fast-charging LIBs due to its high capacity(~1000 mAh g^(−1))and safe lithiation potential(~0.65 V vs.Li^(+)/Li).The Li+diffusion coefficient of Co2VO4 is evaluated by theoretical calculation to be as high as 3.15×10^(-10) cm^(2) s^(−1),proving Co_(2)VO_(4) a promising anode in fast-charging LIBs.A hexagonal porous Co2VO4 nanodisk(PCVO ND)structure is designed accordingly,featuring a high specific surface area of 74.57 m^(2) g^(−1) and numerous pores with a pore size of 14 nm.This unique structure succeeds in enhancing Li^(+) and electron transfer,leading to superior fast-charging performance than current commercial anodes.As a result,the PCVO ND shows a high initial reversible capacity of 911.0 mAh g^(−1) at 0.4 C,excellent fast-charging capacity(344.3 mAh g^(−1) at 10 C for 1000 cycles),outstanding long-term cycling stability(only 0.024% capacity loss per cycle at 10 C for 1000 cycles),confirming the commercial feasibility of PCVO ND in fast-charging LIBs. | Jinghui Ren Zhenyu Wang Peng Xu Cong Wang Fei Gao Decheng Zhao Shupei Liu Han Yang Di Wang Chunming Niu Yusong Zhu Yutong Wu Xiang Liu Zhoulu Wang Yi Zhang | 2022 | Nano-Micro Letters2022，14，1： | 1 |
| 12 | Vegetation regionalization in Guangdong Province显示文摘 | He Shaoyi Chen Shupei | 1963 | Botany and Plante Cology in the Series APR1963，，1： | 1 |
| 13 | Assessing the toxicity of ingested Taihu Lake water on mice via hepatic histopathology and matrix metalloproteinase expression显示文摘 | Zong Y Z Wendi Q Shupei C | 2011 | Ecotoxicology2011，20，5： | 1 |
| 14 | On metamorphic functions that share four values显示文摘 | Wang Shupei | 1993 | J Math Anal Appl1993，173，： | 1 |
| 15 | Preparation and crystallization behaviour of PP/PP-g-MAH/Org-MMT nanocomposite显示文摘 | Weibing Xu Guodong Liang Hongbo Zhai Shupei Tang Guopei Hang Wei-Ping Pan | 2003 | European Polymer Journal2003，，7： | 1 |
| 16 | Effect of Pt/γ-Al2O3 catalyst on nonthermal plasma decomposition of benzeneand byproducts显示文摘 | LI JING HAN SHITONG BAI SHUPEI | 2011 | Environmental Engineering Science2011，28，6： | 1 |
| 17 | Impact of Personal Orientation on Luxury - Brand Purchase Value - an International Investigation 显示文摘 | Tsai ShuPei | 2005 | Joumal of International Market Research2005，47，4： | 1 |
| 18 | Lipolysis and the integrated physiology of lipid energy metabolism显示文摘 | Wang Shupei Soni KG Semache M | 2008 | Mol Genet Metab2008，95，3： | 1 |
| 19 | Risk assessment of polycyclic aromatic hydrocarbons in aquatic ecosystems 显示文摘 | WU Bing ZHANG Rui CHENG Shupei | 2011 | Ecotoxicology2011，20，： | 1 |
| 20 | On the sectorial oscillation theory of f' + Af : 0 显示文摘 | WANG Shupei | 1994 | Annales Academie Scientiarum Fennice Series A I Mathematica Dissertationes1994，92，： | 1 |
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