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| 1 | 不同提取方法山苍子油的化学成分与抗氧化活性分析显示文摘【目的】比较不同提取方法对山苍子油提取效率与抗氧化活性的影响,通过GC-MS分析,研究山苍子油成分构成与抗氧化活性的内在关联,分析山苍子油抗氧化活性的主要物质基础。【方法】采用液液萃取(分别加以超声波与磁化辅助处理)、二氧化碳超临界萃取以及水蒸气蒸馏法提取山苍子油,DPPH法比较不同提取方式对山苍子油抗氧化活力的影响,GC-MS分析山苍子油的主要成分,并以1,4-二溴苯为内标,采用面积归化法解析主要成分的相对含量。【结果】山苍子油具有显著的抗氧化活性。液液萃取法的提取效率与山苍子油抗氧化活性最高,超声波辅助处理油得率最高,为26.3%,磁化辅助处理抗氧化活性最优,达到31.22 mg·mL^-1(IC50),二氧化碳超临界萃取法次之,抗氧化活性为56.95 mg·mL^-1(IC50),水蒸气蒸馏法抗氧化活性最弱,为64.95 mg·mL^-1(IC50);GC-MS分析检测出122种以上的化合物,包括12种脂肪酸,16种萜烯,18种含氧萜烯以及其他微量化合物包括烯烃、醇、酮类与烷烃等。GC-MS分析结果表明:山苍子干果主要成分为饱和脂肪酸(月桂酸等)、不饱和脂肪酸(9,12-十八碳二烯酸、9-十八碳烯酸等)以及α蒎烯、β蒎烯、柠檬烯等萜烯、氧化萜烯类物质。液液萃取法得到的山苍子油脂肪酸含量(53.51%-66.61%)明显高于二氧化碳超临界萃取(17.64%)与水蒸气蒸馏法(8.1%),而二氧化碳超临界萃取与水蒸气蒸馏法得到的萜烯、氧化萜烯类含量分别达到29.37%和17.69%,明显高于液液萃取法(6.49%-9.75%)。【结论】磁化辅助处理有利于抗氧化活性物质的提取,且不饱和脂肪酸的含量最高;超声波辅助处理可以提升油的得率;水蒸气蒸馏法和超临界流体萃取法更有利于萜烯类化合物的获取;联系到水蒸气蒸馏法得到的山苍子油富含萜烯化合物却显示较低的抗氧化活性(64.95 mg·mL^-1),色谱级的柠檬醛抗氧化活性最低(74.33 mg·mL^-1),因此推测脂肪酸成分(特别是不饱和脂肪酸)是山苍子油抗氧化活性的主要物质基础。 | 李芳 Yaru Song Howard Holness Kevin O’Shea | 2015 | 林业科学2015，51，3： | 14 |
| 2 | An epigenomic approach to therapy for tamoxifen-resistant breast cancer显示文摘Tamoxifen 是为雌激素受体 alpha 的前线治疗(ERα) 在绝经前的女人的积极的胸肿瘤。然而,到 tamoxifen 的抵抗发生在许多病人。嗯仍然与获得的 tamoxifen 抵抗在乳癌房间的生长起一个关键作用,建议那 ERα为治疗仍然是一个有效目标 tamoxifen 抵抗(Tam-R ) 乳癌。以识别 ERα 的新奇管理者;发信号,通过对 histone 甲基修饰词的一幅小规模的 siRNA 屏幕,我们发现了 WHSC1, histone H3K36 methyltransferase, ERα 的一个积极管理者;在乳癌房间发信号。我们证明 WHSC1 被招募到 ERα由 BET 蛋白质 BRD3/4 的基因,并且便于 ERα基因表示。小分子的赌注蛋白质禁止者 JQ1 potently 压制了经典 ERα发信号的小径和在文化的 Tam-R 乳癌房间的生长。用一个 Tam-R 乳癌异种皮移植老鼠模型,我们与 JQ1 和 ER degrader fulvestrant 由 JQ1 和联合治疗的强壮的长持续的效果在 vivo 反胸癌症活动示威了。一起拿,我们提供 epigenomic 蛋白质 BRD3/4 和 WHSC1 是雌激素受体发信号的必要管理者并且是为 Tam-R 乳癌的治疗的新奇治疗学的目标的证据。 | Qin Feng Zheng Zhang Martin J Shea Chad J Creighton Cristian Coarfa Susan G Hilsenbeck Rainer Lanz Bin He Lei Wang Xiaoyong Fu Agostina Nardone Yongcheng Song James Bradner Nicholas Mitsiades Constantine S Mitsiades C Kent Osborne Rachel Schiff Bert W O'Malley | 2014 | Cell Research2014，24，7： | 10 |
| 3 | Spectral selectivity in infrared thermal detection显示文摘A review is made of the physics and technology of spectrally selective thermal detectors,especially those operating at non-cryogenic temperatures.The background radiation noise fluctuations are rederived for arbitrary spectral characteristics.Infrared absorption due to phonons and free carriers is discussed followed by a review of published works on artificial infrared absorption materials such as patterned grids,nanoparticles,plasmonic structures,metamaterials and others.Subsequently,the literature of the spectral characteristics of broadband thermal detectors and spectrally selective thermal detectors is reviewed.Finally,the authors speculate on the directions that future research and development in the area will take regarding architectures,sensitivity and spectral characteristics. | Joseph J Talghader Anand S Gawarikar Ryan P Shea | 2012 | Light(Science & Applications)2012，1，1： | 6 |
| 4 | Optimization of micropatterned poly(lactic-coglycolic acid) films for enhancing dorsal root ganglion cell orientation and extension显示文摘Nerve conduits have been a viable alternative to the ‘gold standard' autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion(DRG) cells on poly(lactic-co-glycolic acid)(PLGA) films has not been previously conducted. Photolithography and micro-molding were used to define various combinations of ridge/groove dimensions on PLGA films. The DRG cells obtained from chicken embryos were cultured on micropatterned PLGA films for cell orientation and migration evaluation. Biodegradation of the films occurred during the test period, however, this did not cause deformation or distortion of the micropatterns. Results from the DRG cell orientation test suggest that when the ridge/groove ratio equals 1(ridge/groove width parameters are equal, i.e., 10 μm/10 μm(even)), the degree of alignment depends on the size of the ridges and grooves, when the ratio is smaller than 1(groove controlled) the alignment increases as the ridge size decreases, and when the ratio is larger than 1(ridge controlled), the alignment is reduced as the width of the grooves decreases. The migration rate and neurite extension of DRG neurons were greatest on 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films. Based on the data, the 10 μm/10 μm and 30 μm/30 μm micropatterned PLGA films are the optimized ridge/groove surface patterns for the construction of nerve repair devices. | Ching-Wen Li Brett Davis Jill Shea Himanshu Sant Bruce Kent Gale Jayant Agarwal | 2018 | Neural Regeneration Research2018，13，1： | 5 |
| 5 | 蛋白酶和木聚糖酶对育肥猪的生长性能、营养消化率以及粪便气味的影响显示文摘菜籽粕(RSM)和小麦干酒糟及其可溶物(DDGS)作为副产物可应用到猪的日粮中。然而,与小麦和豆粕型日粮相比,RSM和DDGS中含有较高的非淀粉多糖(NSP),其可限制猪对日粮的有效利用。采用2×2因素试验设计研究生长育肥猪饲粮中添加木聚糖酶(0和200mg·kg-1)和蛋白酶(0和200mg·kg-1)对其生长性能、胴体品质、表观回肠消化率、全消化道养分消化率及粪便气体排放的影响及两种酶之间的相互作用。试验1,对猪的生长性能进行评估。将体重为34.2±2.1kg的育肥猪128头随机分配到4个处理组:以DDGS(300g·kg-1)和RSM(210g·kg-1)的基础日粮;基础日粮中添加蛋白酶200mg·kg-1;基础日粮中添加木聚糖酶200mg·kg-1;基础日粮中添加蛋白酶200mg·kg-1和木聚糖酶200mg·k-1。试验1中,在生长育肥-出栏期间(0~出栏),日粮中添加蛋白酶的试验猪较不添加蛋白酶的试验猪相比具有较低的平均日增重(ADG)(P〈0.001)。生长阶段(0—28d),蛋白酶和木聚糖酶对猪的日均采食量(ADFI,P〈0.01)和体重(BW,P〈0.01)的影响存在相互作用。在育肥阶段(28d~出栏),日粮中添加蛋白酶和木聚糖酶的试验猪与仅添加一种没酶的试验猪相比具有较低的ADFI和BW。然而,蛋白酶和木聚糖酶的相互作用在生长期间(0~28d)内并不显著。试验2,选择体重78±2.3kg公猪24只,将其置于代谢笼中进行代谢试验,试验期间按照试验l中的日粮进行饲喂。木聚糖酶和蛋白酶对总能(GE)中的AID存在相互作用(P〈0.05)。与对照组相比,饲粮中添加蛋白酶可以增加GE中的AID,但饲粮中添加两种酶时可降低GE中的AID。饲粮中添加木聚糖酶交不添加木聚糖组相比,猪粪便的臭气排放量降低(598和1306OuE/m3;P〈0.05)。研究表明,蛋白酶可提高GE的AID;木聚糖酶可以减少粪便异味的排放,然而当生长猪育肥以RSM和DDGS为基础饲粮时,两种酶均不能提高其生长性能。 | O'Shea C J Mc Alpine P O Solan P Curran T Varley P F Walsh A M Doherty J V O | 2014 | 饲料博览2014，0，10： | 4 |
| 6 | 空间杆系结构的智能生成与设计显示文摘计算机已成功应用于大型空间结构的分析、设计与施工图绘制,但如何利用计算机模拟人脑进行方案阶段的概念设计还面临巨大挑战。基于计算生(合)成理论和算法,构建了数值化的空间杆系结构概念设计方法。该方法通过结构形状语法的分类与定义、模拟退火优化算法的改进,可实现在三维空间内搜寻满足设计条件的'最优'结构设计方案。由于数值化概念设计方法在结构构型方面综合了结构拓扑构建、形状确定、截面尺寸选择等参数,在结构设计方面综合了建筑美观性、结构安全经济性、施工便捷性等要求,因而由此生成、设计并实现的空间杆系结构将在结构整体性能和整体造价上优于按传统设计方法或优化设计方法所得到的结构。荷兰阿姆斯特丹'数字华盖'和伦敦Paternoster Square日晷支架的设计与建造表明,该方法可成功应用于概念设计或传统或新颖的空间杆系结构。 | 赵宪忠 SHEA Knstina | 2010 | 建筑结构学报2010，31，9： | 4 |
| 7 | Delayed diagnosis of 22q11.2 deletion syndrome in an adult Chinese lady显示文摘 | SHEA Yat-fung LEE Chi-ho Harinder Gill CHOW Wing-sun LAM Yui-ming LUK Ho-ming LAM Stephen Tak-sum CHU Leung-wing | 2012 | Chinese Medical Journal2012，，16： | 3 |
| 8 | A new technique for Instantaneous frequency rate estimation显示文摘 | Peter O'Shea | 2002 | IEEE Signal Processing Letters2002，9，8： | 2 |
| 9 | Alcoholic liver disease显示文摘 | O'Shea RS Dasarathy S McCullough AJ | | Hepatology0，，： | 2 |
| 10 | Community-acquired Klebsiella pneumoniae liver abscess: an emerging infection in Ireland and Europe显示文摘 | R. Moore D. O’Shea T. Geoghegan P. W. G. Mallon G. Sheehan | 2013 | Infection2013，，3： | 2 |
| 11 | 颈动脉硬化进展与病程≥10年的高血压治疗之间的关系:多种族动脉粥样硬化研究显示文摘尚未有前瞻性多种族队列对抗高血压药物类别和动脉硬化进展的相关性进行研究。该研究所有受试者基线均患高血压,定义为血压≥140/90 mm Hg(1mm Hg=0.133kPa)或服用降压药物治疗。在5个时间点评估药物的服用情况和血压水平。通过超声在基线时和平均随访(9.4±0.5)年后测定右颈总动脉的杨氏弹性模量和扩张系数。 | Gepner AD Tedla Y Colangelo LA Tattersall MC Korcarz CE Kaufman JD Liu K Burke GL Shea S Greenland P Stein JH 刘莉 叶鹏 | 2016 | 中华高血压杂志2016，24，11： | 2 |
| 12 | Controlled release of FK506 from micropatterned PLGA films:potential for application in peripheral nerve repair显示文摘After decades of research,peripheral nerve injury and repair still frequently results in paralysis,chronic pain and neuropathies leading to severe disability in patients.Current clinically available nerve conduits only provide crude guidance of regenerating axons across nerve gap without additional functionality.FK506(Tacrolimus),an FDA approved immunosuppressant,has been shown to enhance peripheral nerve regeneration but carries harsh side-effects when delivered systemically.The objective of this study was to develop and evaluate a bioresorbable drug delivery system capable of local extended delivery of FK506 that also provides topological guidance cues to guide axon growth via microgrooves.Photolithography was used to create micropatterned poly(lactide-co-glycolic acid)(PLGA) films embedded with FK506.Non-patterned,10/10 μm(ridge/groove width),and 30/30 μm patterned films loaded with 0,1,and 3 μg/cm2 FK506 were manufactured and characterized.In vitro FK506 rate of release testing indicated that the films are capable of an extended(at least 56 days),controlled,and scalable release of FK506.Neurite extension bioactivity assay indicated that FK506 released from the films(concentration of samples tested ranged between 8.46–19.7 ng/m L) maintained its neural bioactivity and promoted neurite extension similar to control FK506 dosages(10 ng/m L FK506).The multi-functional FK506 embedded,micropatterned poly(lactide-co-glycolic acid) films developed in this study have potential to be used in the construction of peripheral nerve repair devices. | Brett Davis Susan Wojtalewicz Pratima Labroo Jill Shea Himanshu Sant Bruce Gale Jayant Agarwal | 2018 | Neural Regeneration Research2018，13，7： | 2 |
| 13 | MODELING OF INTERRILL EROSION INTHE LOESS PLATEAU OF CHINA显示文摘Based on the field plots data from simulated rainfall experiments, a theoretical model for calculating the kinetic energy of a single raindrop was developed in the Loess Plateau of China Formulae for both single raindrops and rainfall events are presented in this paper.Analysis shows that overland flow and the area producing runoff on the land surface increase with the duration of rainfall, while the effects of raindrop impact on the surface of the hillslope decrease. The rate of interrill erosion decreases gradually with the rainfall duration. A physically-based formula to calculating the area of runoff production is also given in this paper The overland flow and canopy affect jointly the interrill erosion processes. The paper presents an equation to quantitatively evaluate the effect. It is concluded thats (1) the peak rate of interrill erosion is related to rainfall intensity, rainfall kinetic energy, and slope; and (2) the average rate of interrill erosion during an individual time period for variable rainfall intensity varies with the rainfall intensity, rainfall duration and runoff producing area. | Zhanbin LI Mingan SHAO & Zhanli WANG Bing SHEA (Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water resources. Yangling, Shaanxi 712100, China Xi’an University of Technology , Xi’an , Shaanxi 710048, China) | 1999 | International Journal of Sediment Research1999，14，2： | 2 |
| 14 | Endogenous Circadian Rhythm in Vasovagal Response to Head-Up Tilt显示文摘 | Kun Hu Frank A.J.L. Scheer Michael Laker Carolina Smales Steven A. Shea | 2011 | Circulation2011，，9： | 2 |
| 15 | Artifact reduction in true-FISP imaging of the coronary arteries by adjusting imaging frequency显示文摘 | Deshpande VS Shea SM Li D | 2003 | Magn Reson Med2003，49，5： | 2 |
| 16 | 香港大屿山林木群落结构的比较研究显示文摘对不同程度人为干扰条件下(地区建设)香港大屿山林木群落结构(胸围大于0. 3m)进行比较研究。结果显示,随干扰强度梯度的增加林木群落的物种多样性减少,即自然生长植被中的林木群落(山坡)多样性>轻度人为干扰的林木群落(昂坪)多样性>重度人为干扰的林木群落(东涌)多样性。物种组成和优势度等群落结构因子也因人为干扰程度的不同而有所不同。人类活动有意识地给林木群落引进了外来物种成分,并由这些物种组成新的群落,这些外来树种所带来的影响应该从多方面进行考虑研究。 | 张万里 Mark Shea 何立群 李雷鸿 | 2005 | 植物研究2005，25，2： | 2 |
| 17 | Discordant atrial natriuretic peptide and brain natriuretic peptide levels in lone atrial fibrillation显示文摘 | Patrick T. Ellinor Adrian F. Low Kristen K. Patton Marisa A. Shea Calum A. MacRae | 2005 | Journal of the American College of Cardiology2005，，1： | 2 |
| 18 | 企业现金持有决策的影响因素:基于跨国数据的分析显示文摘文章使用跨国数据分析了制度环境与企业现金持有决策影响因素之间的关系。研究发现,不论是发达国家还是发展中国家,杠杆率、分红、流动性、利润率和企业规模都会影响企业的现金持有决策,但是在不同的国家这些影响因素的符号和显著性存在重要区别,这可能是由于不同的制度环境造成的。研究结果发现,杠杆率、分红和企业规模对企业的现金持有决策影响最显著,制度环境对于现金持有决策的确有显著影响,其中最为典型的就是在股东权利保护较差的国家中,企业持有的现金较多。 | 刘璐 Gary Shea | 2014 | 统计与决策2014，30，11： | 2 |
| 19 | Non-viral vector delivery from PEG-hyaluronic acid hydrogels 显示文摘 | Wieland J A Houchin-Ray T L Shea L D | 2007 | J Control Release2007，120，3： | 1 |
| 20 | Simultaneous identification of bacterial virulence genes by negative selection显示文摘 | Hensel M Shea J E Holden D W etal | 1995 | Science1995，269，： | 1 |
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