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| 1 | Conservation and Diversity of MicroRNA-associated Copper-regulatory Networks in Populus trichocarpa显示文摘Plants develop important regulatory networks to adapt to the frequently-changing availability of copper (Cu). However, little is known about miRNA-associated Cu-regulatory networks in plant species other than Arabidopsis. Here, we report that Cu-responsive miRNAs in Populus trichocarpa (Torr. & Gray) include not only conserved miR397, miR398 and miR408, but also Populus-specific miR1444, suggesting the conservation and diversity of Cu-responsive miRNAs in plants. Copper-associated suppression of mature miRNAs is in company with the up-regulation of their target genes encoding Cu-containing proteins in Populus. The targets include miR397-targeted PtLAC5, PtLAC6 and PtLAC110a, miR398- targeted PtCSD1, PtCSD2a and PtCSD2b, miR408-targeted PtPCL1, PtPCL2, PtPCL3 and PtLAC4, and miR1444-targeted PtPPO3 and PtPPO6. Consistently, P. trichocarpa miR408 promoter-directed GUS gene expression is down-regulated by Cu in transgenic tobacco plants. Cu-response elements (CuREs) are found in the promoters of Cu-responsive miRNA genes. We identified 34 SQUAMOSA-promoter binding protein-like (SPL) genes, of which 17 are full-length PtSPL proteins or partial sequences with at least 300 amino acids. Phylogenetic analysis indicates that PtSPL3 and PtSPL4 are CuRE-binding proteins controlling Cu-responsive gene expression. Cu appears to be not involved in the regulation of these transcription factors because neither PtSPL3 nor PtSPL4 is Cu-regulated and no CuRE exists in their promoters. | Shanfa Lu Chenmin Yang Vincent L. Chiang | 2011 | Journal of Integrative Plant Biology2011，53，11： | 15 |
| 2 | Genome-wide analysis and molecular dissection of the SPL gene family in Salvia miltiorrhiza显示文摘SQUAMOSA promoter binding protein-likes(SPLs) are plant-specific transcription factors playing vital regulatory roles in plant growth and development. There is no information about SPLs in Salvia miltiorrhiza(Danshen), a significant medicinal plant widely used in Traditional Chinese medicine(TCM) for >1,700 years and an emerging model plant for TCM studies. Through genome-wide identification and subsequent molecular cloning, we identified a total 15 SmSPLs with divergent sequence features, gene structures, and motifs. Comparative analysis showed sequence conservation between SmSPLs and their Arabidopsis counterparts. A phylogenetic tree clusters SmSPLs into six groups. Many of the motifs identified commonly exist in a group/subgroup, implying their functional redundancy. Eight SmSPLs were predicted and experimentally validated to be targets of miR156/157. SmSPLs were differentially expressed in various tissues of S. milltiorrhiza. The expression of miR156/157-targeted SmSPLs was increased withthe maturation of S. miltiorrhiza, whereas the expression of miR156/157 was decreased, confirming the regulatory roles of miR156/157 in SmSPLs and suggesting the functions of SmSPLs in S. miltiorrhiza development. The expression of miR156/157 was negatively correlated with miR172 during the maturation of S. miltiorrhiza. The results indicate the significance and complexity of SmSPL-, miR156-, and miR172-mediated regulation of developmental timing in S. miltiorrhiza. | Linsu Zhang Bin Wu Degang Zhao Caili Li Fenjuan Shao Shanfa Lu | 2014 | Journal of Integrative Plant Biology2014，56，1： | 13 |
| 3 | Relation between phytohormone level and vascular bridge differentiation in graft union of explanted internode autografting显示文摘Development of the graft union of explanted internode autografting of cucumber ( Cu-cumis sativus Linn.) cultured in vitro is regulated by plant hormones exogenously added to the media. The levels of auxin (indole-3-acetic acid, IAA) and zeatin plus zeatin riboside (Z+ ZR) in graft unions and in 4 parts of the graft union have been analyzed by ELISA assay. | Shanfa Lu Yanru Song | 1999 | Chinese Science Bulletin1999，44，20： | 8 |
| 4 | Identification of m RNA-like non-coding RNAs and validation of a mighty one named MAR in Panax ginseng显示文摘Increasing evidence suggests that long non-coding RNAs(lnc RNAs) play significant roles in plants.However,little is known about lnc RNAs in Panax ginseng C.A.Meyer,an economically significant medicinal plant species.A total of3,688 m RNA-like non-coding RNAs(mlnc RNAs),a class of lnc RNAs,were identified in P.ginseng.Approximately 40% of the identified mlnc RNAs were processed into small RNAs,implying their regulatory roles via small RNA-mediated mechanisms.Eleven mi RNA-generating mlnc RNAs also produced si RNAs,suggesting the coordinated production of mi RNAs and si RNAs in P.ginseng.The mlnc RNA-derived small RNAs might be 21-,22-,or 24-nt phased and could be generated from both or only one strand of mlnc RNAs,or from super long hairpin structures.A full-length mlnc RNA,termed MAR(multiple-function-associated mlnc RNA),was cloned.It generated the most abundant si RNAs.The MAR si RNAs were Researchpredominantly 24-nt and some of them were distributed in a phased pattern.A total of 228 targets were predicted for 71 MAR si RNAs.Degradome sequencing validated 68 predicted targets involved in diverse metabolic pathways,suggesting the significance of MAR in P.ginseng.Consistently,MAR was detected in all tissues analyzed and responded to methyl jasmonate(Me JA) treatment.It sheds light on the function of mlncRNAs in plants. | Meizhen Wang Bin Wu Chao Chen Shanfa Lu | 2015 | Journal of Integrative Plant Biology2015，57，3： | 2 |
| 5 | Are herbal sRNAs really novel precision medicines?显示文摘Dear Editor,In 2012,Zhang et al.(2012)published an interesting and widely cited article in Cell Research.They reported that exogenous plant miR168a could enter mammals through oral food intake to regulate the expression of mammalian LDLRAP1 gene.From then on,cross-kingdom regulation of diet-derived plant miRNAs has generated substantial interest and attracted media to report.Although there is evidence to support this point of view,the authenticity of cross-kingdom regulation of plant miRNAs has been widely questioned.In 2019,Science China Life Sciences published a special topic entitled“Herbal small RNA,novel precision medicine from Mother Nature”,in which the authors tried to demonstrate an idea that herbal sRNAs are novel precision medicines.This is a thought further than cross-kingdom regulation of sRNAs.However,are herbal sRNAs really novel precision medicines? | Shanfa Lu | 2023 | Science China(Life Sciences)2023，66，11： | 1 |
| 6 | Clarification of cinnamoyl Co-enzyme A r-eductase catalysis in monolignol biosynthesis of aspen显示文摘 | LAIGENG L XIAOFEI C SHANFA L | 2005 | Plant and Cell Physiology2005，46，7： | 1 |
| 7 | A genomic and molecular view of wood formation显示文摘 | Li Laigeng Lu Shanfa Chiang V | | 0，，03： | 1 |
| 8 | Occupational stress in workers and managers in steelworks in China显示文摘 | Yu Shanfa Sparks K Cooper CL | 1998 | Int J Stress Management1998，5，4： | 1 |
| 9 | Distinct Roles of Cinnamate 4-hydroxylase Genes in Populus显示文摘 | Lu Shanfa Zhou Yihua Li Laigeng | 2006 | Plant and Cell Physiology2006，，47： | 1 |
| 10 | Stress-responsive microRNAs in Populus显示文摘 | Lu Shanfa Sun Ying-Hsuan Chiang V L | 2008 | Plant J2008，55，1： | 1 |
| 11 | RNA silencing in plants by the expression of siRNA duplexes 显示文摘 | Lu Shanfa Shi Rui Cheng-Chung Tsao | 2004 | Nucleic Acids Research2004，32，21： | 1 |
| 12 | Occupational Stress in Workers and Managers in Steelworks in China显示文摘 | Yu Shanfa Kate Sparks Cary L Cooper | 1998 | Int J Stress Management1998，5，4： | 1 |
| 13 | Application of biological markers in the assessment of paleoenvironments of Chinese non- marine sediments 显示文摘 | Fu Jiamo Sheng Guoying Xu Jiayou Egiinton G Gowar A P Jia Rngfen Fan Shanfa Peng Pingan | 1990 | Org Geochem1990，16，46： | 1 |
| 14 | Investigation of the properties of epoxy resin-modified asphalt mixtures for application to orthotropic bridge decks显示文摘 | CONG Peihang CHEN Shanfa YU Jianying | 2011 | Journal of Applied Polymer Science2011，121，4： | 1 |
| 15 | Specific down-regulation of PAL genes by artificial microRNAs in Populus trichocarpa显示文摘 | Rui Shi Chenmin Yang Shanfa Lu Ronald Sederoff Vincent L. Chiang | 2010 | Planta2010，，6： | 1 |
| 16 | Distinct roles of cin- namate 4-hydroxylase genes in Populus 显示文摘 | Lu'Shanfa Zhou Yihua Li Laigeng | 2006 | Plant Cell Physiol2006，47，7： | 1 |
| 17 | Research on rheological behavior of anion gemini surfactant solution显示文摘 | Tang Shanfa Liu Zhongyun Liu Shuang Hu Xiaodong Wu Ruidong | 2010 | Advanced Materials Research2010，10，146147： | 1 |
| 18 | Psychosocial work environment and well-being: cross-sectional study in a thermal power plant in China 显示文摘 | Yu Shanfa Gu Guizhen Zbou Wenhui | 2008 | Journal of Occupational Health2008，50，： | 1 |
| 19 | Occupational Stress in Workers and Managers in Steelworks in China显示文摘 | Yu Shanfa Kate Sparks Gary L. Cooper | 1998 | International Journal of Stress Management1998，，4： | 1 |
| 20 | Study of Oil-Bearing Drill Cuttings Cleaning and De-Oiling Treatment Method for Shale Gas Reservoirs显示文摘Due to its extensive use in shale gas exploration and development,oil-based drilling fluids generate large amounts of oil-bearing drill cuttings during the drilling process.The large amount of oil-bearing drill cuttings generated during the drilling process can lead to serious secondary contamination.In this study,a wetting agent FSC-6 with good hydrophobic and oleophobic properties was synthesized to construct an efficient oil removal system.For the first time,the mechanism of this system was analyzed by using the theory of adhesion function,interfacial tension and wettability.At the same time,a combined acoustic-chemical treatment process was applied to the wastewater and slag generated after the cleaning of the oil-bearing drill cuttings.The experimental results show that the application of this pollution-free technology can effectively solve the environmental pollution and resource recovery problems of oil-bearing drill cuttings.It meets the standard of drilling chips with oil content less than 2%in SY/T7422-2018“Oil-based drilling fluid drilling chips treatment system for oil and gas drilling equipment”. | Jialuo Rong Shuixiang Xie Huijing Geng Hao Hu Shanfa Tang Yuanpeng Cheng | 2023 | Energy Engineering2023，120，8： | 0 |
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