|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Conservation and Diversity of MicroRNA-associated Copper-regulatory Networks in Populus trichocarpa显示文摘Plants develop important regulatory networks to adapt to the frequently-changing availability of copper (Cu). However, little is known about miRNA-associated Cu-regulatory networks in plant species other than Arabidopsis. Here, we report that Cu-responsive miRNAs in Populus trichocarpa (Torr. & Gray) include not only conserved miR397, miR398 and miR408, but also Populus-specific miR1444, suggesting the conservation and diversity of Cu-responsive miRNAs in plants. Copper-associated suppression of mature miRNAs is in company with the up-regulation of their target genes encoding Cu-containing proteins in Populus. The targets include miR397-targeted PtLAC5, PtLAC6 and PtLAC110a, miR398- targeted PtCSD1, PtCSD2a and PtCSD2b, miR408-targeted PtPCL1, PtPCL2, PtPCL3 and PtLAC4, and miR1444-targeted PtPPO3 and PtPPO6. Consistently, P. trichocarpa miR408 promoter-directed GUS gene expression is down-regulated by Cu in transgenic tobacco plants. Cu-response elements (CuREs) are found in the promoters of Cu-responsive miRNA genes. We identified 34 SQUAMOSA-promoter binding protein-like (SPL) genes, of which 17 are full-length PtSPL proteins or partial sequences with at least 300 amino acids. Phylogenetic analysis indicates that PtSPL3 and PtSPL4 are CuRE-binding proteins controlling Cu-responsive gene expression. Cu appears to be not involved in the regulation of these transcription factors because neither PtSPL3 nor PtSPL4 is Cu-regulated and no CuRE exists in their promoters. | Shanfa Lu Chenmin Yang Vincent L. Chiang | 2011 | Journal of Integrative Plant Biology2011，53，11： | 15 |
| 2 | Genome-wide analysis and molecular dissection of the SPL gene family in Salvia miltiorrhiza显示文摘SQUAMOSA promoter binding protein-likes(SPLs) are plant-specific transcription factors playing vital regulatory roles in plant growth and development. There is no information about SPLs in Salvia miltiorrhiza(Danshen), a significant medicinal plant widely used in Traditional Chinese medicine(TCM) for >1,700 years and an emerging model plant for TCM studies. Through genome-wide identification and subsequent molecular cloning, we identified a total 15 SmSPLs with divergent sequence features, gene structures, and motifs. Comparative analysis showed sequence conservation between SmSPLs and their Arabidopsis counterparts. A phylogenetic tree clusters SmSPLs into six groups. Many of the motifs identified commonly exist in a group/subgroup, implying their functional redundancy. Eight SmSPLs were predicted and experimentally validated to be targets of miR156/157. SmSPLs were differentially expressed in various tissues of S. milltiorrhiza. The expression of miR156/157-targeted SmSPLs was increased withthe maturation of S. miltiorrhiza, whereas the expression of miR156/157 was decreased, confirming the regulatory roles of miR156/157 in SmSPLs and suggesting the functions of SmSPLs in S. miltiorrhiza development. The expression of miR156/157 was negatively correlated with miR172 during the maturation of S. miltiorrhiza. The results indicate the significance and complexity of SmSPL-, miR156-, and miR172-mediated regulation of developmental timing in S. miltiorrhiza. | Linsu Zhang Bin Wu Degang Zhao Caili Li Fenjuan Shao Shanfa Lu | 2014 | Journal of Integrative Plant Biology2014，56，1： | 13 |
| 3 | Relation between phytohormone level and vascular bridge differentiation in graft union of explanted internode autografting显示文摘Development of the graft union of explanted internode autografting of cucumber ( Cu-cumis sativus Linn.) cultured in vitro is regulated by plant hormones exogenously added to the media. The levels of auxin (indole-3-acetic acid, IAA) and zeatin plus zeatin riboside (Z+ ZR) in graft unions and in 4 parts of the graft union have been analyzed by ELISA assay. | Shanfa Lu Yanru Song | 1999 | Chinese Science Bulletin1999，44，20： | 8 |
| 4 | Identification of m RNA-like non-coding RNAs and validation of a mighty one named MAR in Panax ginseng显示文摘Increasing evidence suggests that long non-coding RNAs(lnc RNAs) play significant roles in plants.However,little is known about lnc RNAs in Panax ginseng C.A.Meyer,an economically significant medicinal plant species.A total of3,688 m RNA-like non-coding RNAs(mlnc RNAs),a class of lnc RNAs,were identified in P.ginseng.Approximately 40% of the identified mlnc RNAs were processed into small RNAs,implying their regulatory roles via small RNA-mediated mechanisms.Eleven mi RNA-generating mlnc RNAs also produced si RNAs,suggesting the coordinated production of mi RNAs and si RNAs in P.ginseng.The mlnc RNA-derived small RNAs might be 21-,22-,or 24-nt phased and could be generated from both or only one strand of mlnc RNAs,or from super long hairpin structures.A full-length mlnc RNA,termed MAR(multiple-function-associated mlnc RNA),was cloned.It generated the most abundant si RNAs.The MAR si RNAs were Researchpredominantly 24-nt and some of them were distributed in a phased pattern.A total of 228 targets were predicted for 71 MAR si RNAs.Degradome sequencing validated 68 predicted targets involved in diverse metabolic pathways,suggesting the significance of MAR in P.ginseng.Consistently,MAR was detected in all tissues analyzed and responded to methyl jasmonate(Me JA) treatment.It sheds light on the function of mlncRNAs in plants. | Meizhen Wang Bin Wu Chao Chen Shanfa Lu | 2015 | Journal of Integrative Plant Biology2015，57，3： | 2 |
| 5 | Are herbal sRNAs really novel precision medicines?显示文摘Dear Editor,In 2012,Zhang et al.(2012)published an interesting and widely cited article in Cell Research.They reported that exogenous plant miR168a could enter mammals through oral food intake to regulate the expression of mammalian LDLRAP1 gene.From then on,cross-kingdom regulation of diet-derived plant miRNAs has generated substantial interest and attracted media to report.Although there is evidence to support this point of view,the authenticity of cross-kingdom regulation of plant miRNAs has been widely questioned.In 2019,Science China Life Sciences published a special topic entitled“Herbal small RNA,novel precision medicine from Mother Nature”,in which the authors tried to demonstrate an idea that herbal sRNAs are novel precision medicines.This is a thought further than cross-kingdom regulation of sRNAs.However,are herbal sRNAs really novel precision medicines? | Shanfa Lu | 2023 | Science China(Life Sciences)2023，66，11： | 1 |
| 6 | A genomic and molecular view of wood formation显示文摘 | Li Laigeng Lu Shanfa Chiang V | | 0，，03： | 1 |
| 7 | Distinct Roles of Cinnamate 4-hydroxylase Genes in Populus显示文摘 | Lu Shanfa Zhou Yihua Li Laigeng | 2006 | Plant and Cell Physiology2006，，47： | 1 |
| 8 | Stress-responsive microRNAs in Populus显示文摘 | Lu Shanfa Sun Ying-Hsuan Chiang V L | 2008 | Plant J2008，55，1： | 1 |
| 9 | RNA silencing in plants by the expression of siRNA duplexes 显示文摘 | Lu Shanfa Shi Rui Cheng-Chung Tsao | 2004 | Nucleic Acids Research2004，32，21： | 1 |
| 10 | Specific down-regulation of PAL genes by artificial microRNAs in Populus trichocarpa显示文摘 | Rui Shi Chenmin Yang Shanfa Lu Ronald Sederoff Vincent L. Chiang | 2010 | Planta2010，，6： | 1 |
| 11 | Distinct roles of cin- namate 4-hydroxylase genes in Populus 显示文摘 | Lu'Shanfa Zhou Yihua Li Laigeng | 2006 | Plant Cell Physiol2006，47，7： | 1 |
| 12 | Characteristics of Salvia miltiorrhiza methylome and the regulatory mechanism of DNA methylation in tanshinone biosynthesis显示文摘Salvia miltiorrhiza is a model medicinal plant with significant economic and medicinal value.Its roots produce a group of diterpenoid lipophilic bioactive components,termed tanshinones.Biosynthesis and regulation of tanshinones has attracted widespread interest.However,the methylome of S.miltiorrhiza has not been analysed and the regulatory mechanism of DNA methylation in tanshinone production is largely unknown.Here we report single-base resolution DNA methylomes from roots and leaves.Comparative analysis revealed differential methylation patterns for CG,CHG,and CHH contexts and the association between DNA methylation and the expression of genes and small RNAs.Lowly methylated genes always had higher expression levels and 24-nucleotide sRNAs could be key players in the RdDM pathway in S.miltiorrhiza.DNA methylation variation analysis showed that CHH methylation contributed mostly to the difference.Go enrichment analysis showed that diterpenoid biosynthetic process was significantly enriched for genes with downstream overlapping with hypoCHHDMR in July_root when comparing with those in March_root.Tanshinone biosynthesis-related enzyme genes,such as DXS2,CMK,IDI1,HMGR2,DXR,MDS,CYP76AH1,2OGD25,and CYP71D373,were less CHH methylated in gene promoters or downstream regions in roots collected in July than those collected in March.Consistently,gene expression was up-regulated in S.miltiorrhiza roots collected in July compared with March and the treatment of DNA methylation inhibitor 5-azacytidine significantly promoted tanshinone production.It suggests that DNA methylation plays a significant regulatory role in tanshinone biosynthesis in S.miltiorrhiza through changing the levels of CHH methylation in promoters or downstreams of key enzyme genes. | Jiang Li Caili Li Yuxing Deng Hairong Wei Shanfa Lu | 2023 | Horticulture Research2023，10，7： | 0 |
| 13 | Chromosome-level genome assembly of Salvia miltiorrhiza with orange roots uncovers the role of Sm2OGD3 in catalyzing 15,16-dehydrogenation of tanshinones显示文摘Salvia miltiorrhiza is well known for its clinical practice in treating heart and cardiovascular diseases.Its roots,used for traditional Chinese medicine materials,are usually brick-red due to accumulation of red pigments,such as tanshinone IIA and tanshinone I.Here we report a S.miltiorrhiza line(shh)with orange roots.Compared with the red roots of normal S.miltiorrhiza plants,the contents of tanshinones with a single bond at C-15,16 were increased,whereas those with a double bond at C-15,16 were significantly decreased in shh.We assembled a high-quality chromosome-level genome of shh.Phylogenomic analysis showed that the relationship between two S.miltiorrhiza lines with red roots was closer than the relationship with shh.It indicates that shh could not be the mutant of an extant S.miltiorrhiza line with red roots.Comparative genomic and transcriptomic analyses showed that a 1.0 kb DNA fragment was deleted in shh Sm2OGD3m.Complementation assay showed that overexpression of intact Sm2OGD3 in shh hairy roots recovered furan D-ring tanshinone accumulation.Consistently,in vitro protein assay showed that Sm2OGD3 catalyzed the conversion of cyptotanshinone,15,16-dihydrotanshinone I and 1,2,15,16-tetrahydrotanshinone I into tanshinone IIA,tanshinone I and 1,2-dihydrotanshinone I,respectively.Thus,Sm2OGD3 functions as tanshinone 15,16-dehydrogenase and is a key enzyme in tanshinone biosynthesis.The results provide novel insights into the metabolic network of medicinally important tanshinone compounds. | Xian Pan Yujie Chang Caili Li Xiaoxiao Qiu Xinyun Cui Fanqi Meng Sixuan Zhang Xian’en Li Shanfa Lu | 2023 | Horticulture Research2023，10，6： | 0 |
| 14 | CAS DEVELOPMENT STRATEGY IN MACROBIOLOGY显示文摘Macrobiology. an important area of biology, has close relations with industry, agriculture, science, education, foreign affairs and foreign trade. It offers a theoretical foundation for solving many problems confronting the human beings in fields such as human population, food, the environment, resources and medical care, and provides scientific basis for major decision-making by the State. In this paper, discussed are the role of macrobiology in science, the economy and social development, the trend of development of macrobiology in the 21st century, and the present situation of macrobiology at the CAS. Lastly, the development strategy and main countermeasures of this discipline are also proposed. | Tong Fengqin, Niu Deshui, Lou Zhiping, Yao Qingxiao Lu Shanfa(Nature Social Coordination Development Bureau, CAS) | 1996 | Bulletin of the Chinese Academy of Sciences1996，10，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员