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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | Evaluation of a rapid bedside assay for detection of serum cardiac troponin T 显示文摘 | Antman E M Grudzien C Sacks D B | 1995 | JAMA1995，273，： | 1 |
| 3 | Density of platelet collagen receptors glycoprotein VI and alpha2 betal and prior myocardial infarction in human subjects-a pilot study显示文摘 | Samaha FF Hibbard C Sacks J | 2005 | Med Sci Monit2005，11，： | 1 |
| 4 | Long-term clinical effect of hemodynamically optimized cardiac resynchronization therapy in patients with heart failure and ventricular conduction delay显示文摘 | Auricchio A Stellbrink C Sack S | 2002 | J Am Coll Cardiol2002，39，12： | 1 |
| 5 | Carbothermal Reduction Synthesis of Nanoerystalline Zirconium Carbide and Hafnium Car- bide Powders Using Solution-Derived Precursors 显示文摘 | Sacks M D Wang C A Yang Z | 2004 | Journal of Materials Science2004，39，19： | 1 |
| 6 | Long-term clinical effect of hemodynamically optimized cardiac resynchronization therapy in patients with heart failure and ventricular conduction delay显示文摘 | Auricchio A Stellbrink C Sack S | 2002 | J Am Coll Am J Cardiol2002，39，： | 1 |
| 7 | Diarrheal epidemics in Dhaka,Bangladesh, during three consecutive floods: 1988,1998,and 2004显示文摘 | Schwartz B S Harris J B Khan A I Larocque R C Sack D A Malek M A | 2006 | Am J Trop Med Hyg2006，74，： | 1 |
| 8 | Exchange model and exchange object concepts for implementation of national BIM standards 显示文摘 | Eastman C M Jeong Y S Sacks R | 2010 | Jonrnal of Computing in Civil Engineering2010，，2： | 1 |
| 9 | Randomized versus historical controls for clinical trials显示文摘 | Sacks H Chalmers T C Smith H J | 1982 | Am J Med1982，72，： | 1 |
| 10 | Long term clinical effect of hemodynamically optimized cardiac resynchronization therapy in patients with heart failure and ventricular conduction delay显示文摘 | Auricchio A Stellbrink C Sack S | 2002 | J Am Coll Cardiol2002，39，12： | 1 |
| 11 | The mitochondrial biogenesis regulatory program in cardiac adaptation to ischemia- a putative target for therapeutic intervention 显示文摘 | McLeod C J Pagel I Sack MN | 2005 | Trends Cardiovasc Med2005，15，3： | 1 |
| 12 | Transient expres- sion of a tumor-specific single-chain fragment and a chimeric anti- body in tobacco leaves显示文摘 | VAQUERO C SACK M CHANDLER J | 1999 | Proceedings of the National Academy of Sciences1999，96，11： | 1 |
| 13 | Long-term clinical effect of hemodynamically opti mized cardiac resynehronization therapy in patients with heart failure and ventricular conduction delay显示文摘 | AURICCHIO A STELLBRINK C SACK S | 2002 | J Am Coll Cardiol2002，39，： | 1 |
| 14 | Crystallographic structures of the ligand-binding domains of the androgen receptor and its T877A mutant complexed with the natural agonist dihydrotestosteronc 显示文摘 | SACK J S KISH K F WANG C | 2001 | Proc Natl Acad Sci USA2001，98，9： | 1 |
| 15 | Thrombin-mediated increases in cytosolic involve different mechanisms in human pulmonary artery smooth muscle and endothelial cells显示文摘 | Sacks R S Firth A L Remillard C V | 2008 | Am J Physiol Lung Cell Mol Physiol2008，295，： | 1 |
| 16 | Requirements for BIM platforms in the concrete reinforcement supply chain显示文摘 | Aram S Eastman C Sacks R | | 0，，： | 1 |
| 17 | Product data modeling using GTPPM-A case study 显示文摘 | Lee G H Sacks R Eastman C | 2007 | Automation in Construction2007，16，： | 1 |
| 18 | Effect on cancer cells of plasmids that express antisense RNA of human papillomavirus type 18显示文摘 | Steele C Sacks PG Adler-Storthz K | 1992 | Cancer Res1992，52，17： | 1 |
| 19 | Non - high - density lipoprotein cholesterol and apolipoprotein B in the prediction of coronary heart disease in men显示文摘 | Pischon T Cirman C J Sacks FM | 2005 | Circulation2005，112，22： | 1 |
| 20 | The Pacing Therapies for Congestive Heart Failure (PATH-CHF) Study:rationale,design,and endpoints of a prospective randomized multicenter study显示文摘 | Auricchio A Stellbrink C Sack S | | American Journal of Cardiology0，，： | 1 |
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