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| 1 | Anti-hepatitis C virus potency of a new autophagy inhibitor using human liver slices model显示文摘AIM: To evaluate the antiviral potency of a new antihepatitis C virus(HCV) antiviral agent targeting the cellular autophagy machinery. METHODS: Non-infected liver slices, obtained from human liver resection and cut in 350 μm-thick slices(2.7 × 106 cells per slice) were infected with cell culture-grown HCV Con1b/C3 supernatant(multiplicity of infection = 0.1) cultivated for up to ten days. HCV infected slices were treated at day 4 post-infection with GNS-396 for 6 d at different concentrations. HCV replication was evaluated by strand-specific real-time quantitative reverse transcription- polymerase chain reaction. The infectivity titers of supernatants were evaluated by foci formation upon inoculation into naive Huh-7.5.1 cells. The cytotoxic effect of the drugs was evaluated by lactate dehydrogenase leakage assays. RESULTS: The antiviral efficacy of a new antiviral drug, GNS-396, an autophagy inhibitor, on HCV infection of adult human liver slices was evidenced in a dosedependent manner. At day 6 post-treatment, GNS-396 EC50 was 158 nmol/L without cytotoxic effect(compared to hydroxychloroquine EC50 = 1.17 μmol/L).CONCLUSION: Our results demonstrated that our ex vivo model is efficient for evaluation the potency of autophagy inhibitors, in particular a new quinoline derivative GNS-396 as antiviral could inhibit HCV infection in a dosedependent manner without cytotoxic effect. | Sylvie Lagaye Sonia Brun Jesintha Gaston Hong Shen Ruzena Stranska Claire Camus Clarisse Dubray Géraldine Rousseau Pierre-Philippe Massault Jerome Courcambeck Firas Bassisi Philippe Halfon Stanislas Pol | 2016 | World Journal of Hepatology2016，8，21： | 5 |
| 2 | Ultrastructure and histochemistry of rat myocardial capillary endothelial cells in response to diabetes and hypertension显示文摘Insufficient growth and rarefaction of capillaries, followed by endothelial dysfunction may represent one of the mostcritical mechanisms involved in heart damage. In this study we examined histochemical and ultrastructural changes inmyocardial capillary endothelium in two models of heart failure streptozotocin-induced diabetes mellitus (STZ) and NO-deficient hypertension in male Wistar rats. Diabetes was induced by a single i.v. dose of STZ (45 mg/kg) and chronic 9-week stage was analysed. To induce NO-deficient hypertension, animals were treated with inhibitor of NO synthase L-nitroarginine methylester (L-NAME) (40 mg/kg) for 4 weeks. Left ventricular tissue was processed for enzyme cata-lytic histochemistry of capillary alkaline phosphatase (AlPh), dipeptidyl peptidase IV (DPP IV), and endothelial NOsynthase/NADPH-diaphorase (NOS) and for ultrastructural analysis. In diabetic and hypertensive rats, lower/absentAlPh and DPP IV activities were found in focal micro-areas. NOS activity was significantly reduced and persisted onlylocally. Quantitative evaluation demonstrated reduction of reaction product intensity of AlPh, DPP and NOS by 49.50%,74.36%, 20.05% in diabetic and 62.93%, 82.71%, 37.65% in hypertensive rats. Subcellular alterations of endothelialcells were found in heart of both groups suggesting injury of capillary function as well as compensatory processes.Endothelial injury was more significant in diabetic animals, in contrast the adaptation was more evident in hypertensiveones. Concluding: both STZ-induced diabetes- and NO-deficient hypertension-related cardiomyopathy were accompa-nied by similar features of structural remodelling of cardiac capillary network manifested as angiogenesis and angiopathy.The latter was however, predominant and may accelerate disappearance of capillary endothelium contributing to myo-cardial dysfunction. | Ludmila OKRUHLICOVA Narcis TRIBULOVA1 Peter WEISMANN Ruzena SOTNIKOVA | 2005 | Cell Research2005，15，7： | 4 |
| 3 | Bioconversion of organic waste by the year 2010:to recycle elements and save energy显示文摘 | Gajdo(s) Ruzena | 1998 | Resources Conservation and Recycling1998，23，12： | 1 |
| 4 | Segmentation of Range Im- ages as the Seareh fiJr Geometric Parametric Models显示文摘 | Ales Leonardis Alok Gupta Ruzena Bajesy | 1995 | International Journal of Computer Vision1995，14，2： | 1 |
| 5 | Scaling the dynamic ap- proach to path planning and control: competition among beha- vioral constraints显示文摘 | Edward W L Henrik I Ruzena B | 1999 | The International Journal of Robotics Research1999，18，1： | 1 |
| 6 | Recovery of parametric models from range image:the case for superquadrics with global deformations显示文摘 | Solina Franc Bajcsy Ruzena | 1990 | IEEE Transactions on Pattern Analysis and Machine Intelligence1990，12，2： | 1 |
| 7 | Multiresolution Elastic Matching显示文摘 | Kovacic S | 1989 | Computer Vision Graphics and Image Processing1989，46，1： | 1 |
| 8 | Scaling the dynamic approach to path planning and control: competition among behavioral constraints 显示文摘 | EDWARD W L HENRIK I RUZENA B | 1999 | The International Journal of Robotics Research1999，18，1： | 1 |
| 9 | Single-View-Point Omni-direc-tional catadioptric cone mirror imager显示文摘 | LIN Shih-Schon Ruzena Bajcsy | 2006 | IEEE Transactions on Pattern Analysis and Machine Intelligence2006，28，5： | 1 |
| 10 | Bioconversion of organic waste by the year 2010: to recycle elements and save energy显示文摘 | Ruzena Gajdos | 1998 | Resources Conservation and Recycling1998，23，： | 1 |
| 11 | International Bias Detected in Judging Rhythmic Gynmastics Competition at Sydney-2000 Olympic Games显示文摘 | RUZENA POPOVIC | 2000 | Physical Edu Sport2000，1，7： | 1 |
| 12 | Single-View-Point Omni-di- rectional Catadioptric Cone Mirror Imager显示文摘 | Lin Shih-Schon Ruzena Bajcsy | 2006 | IEEE Trans- actions on Pattern Analysis and Machine Intelligence2006，28，5： | 1 |
| 13 | Sealing the dynamic approach to path planning and control: competition among behavioral constraints显示文摘 | Edward W L Henrik I Ruzena B | 1999 | The International Journal of Robotics Research1999，18，1： | 1 |
| 14 | High prevalence of unrecognized sleep apnoea in drug-resistant hypertension显示文摘 | Alexander G. Logan Sandra M. Perlikowski Andrew Mente Andras Tisler Ruzena Tkacova Mitra Niroumand Richard S. T. Leung T. Douglas Bradley | 2001 | Journal of Hypertension2001，，12： | 1 |
| 15 | Effect of Moisture on the Insulation Condition of Twisted Coils显示文摘 | Bohumil Kotlarik Ruzena Vankova Zuzana Filova | 2014 | Journal of Energy and Power Engineering2014，8，4： | 0 |
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