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| 1 | RAB31 marks and controls an ESCRT-independent exosome pathway显示文摘Exosomes are generated within the multivesicular endosomes(MVEs)as intraluminal vesicles(ILVs)and secreted during the fusion of MVEs with the cell membrane.The mechanisms of exosome biogenesis remain poorly explored.Here we identify that RAB31 marks and controls an ESCRT-independent exosome pathway.Active RAB31,phosphorylated by epidermal growth factor receptor(EGFR),engages flotillin proteins in lipid raft microdomains to drive EGFR entry into MVEs to form ILVs,which is independent of the ESCRT(endosomal sorting complex required for transport)machinery.Active RAB31 interacts with the SPFH domain and drives ILV formation via the Flotillin domain of flotillin proteins.Meanwhile,RAB31 recruits GTPase-activating protein TBC1D2B to inactivate RAB7,thereby preventing the fusion of MVEs with lysosomes and enabling the secretion of ILVs as exosomes.These findings establish that RAB31 has dual functions in the biogenesis of exosomes:driving ILVs formation and suppressing MVEs degradation,providing an exquisite framework to better understand exosome biogenesis. | Denghui Wei Weixiang Zhan Ying Gao Liyan Huang Run Gong Wen Wang Ruhua Zhang Yuanzhong Wu Song Gao Tiebang Kang | 2021 | Cell Research2021，31，2： | 12 |
| 2 | Two gap-free reference genomes and a global view of the centromere architecture in rice显示文摘Rice(Oryza sativa),a major staple throughout the world and a model system for plant genomics and breeding,was the first crop genome sequenced almost two decades ago.However,reference genomes for all higher organisms to date contain gaps and missing sequences.Here,we report the assembly and analysis of gap-free reference genome sequences for two elite O.sativa xian/indica rice varieties,Zhenshan 97 and Minghui 63,which are being used as a model system for studying heterosis and yield.Gap-free reference genomes provide the opportunity for a global view of the structure and function of centromeres.We show that all rice centromeric regions share conserved centromere-specific satellite motifs with different copy numbers and structures.In addition,the similarity of CentO repeats in the same chromosome is higher than across chromosomes,supporting a model of local expansion and homogenization.Both genomes have over 395 non-TE genes located in centromere regions,of which∼41%are actively transcribed.Two large structural variants at the end of chromosome 11 affect the copy number of resistance genes between the two genomes.The availability of the two gap-free genomes lays a solid foundation for further understanding genome structure and function in plants and breeding climate-resilient varieties. | Jia-Ming Song Wen-Zhao Xie Shuo Wang Yi-Xiong Guo Dal-Hoe Koo Dave Kudrna Chenbo Gong Yicheng Huang Jia-Wu Feng Wenhui Zhang Yong Zhou Andrea Zuccolo Evan Long Seunghee Lee Jayson Talag Run Zhou Xi-Tong Zhu Daojun Yuan Joshua Udall Weibo Xie Rod AWing Qifa Zhang Jesse Poland Jianwei Zhang Ling-Ling Chen | 2021 | Molecular Plant2021，14，10： | 9 |
| 3 | Construction of a BAC library from cucumber (Cucumis sativus L.) and identification of linkage group specific clones显示文摘A bacterial artificial chromosome (BAC) library consisting of 19,200 clones with an average insert size of 105 kb has been constructed from a cucumber (Cucumis sativus L.) inbred line S94,derived from a cultivar in North China. The entire library was equivalent to approximately 5 haploid cucumber genomes. To facilitate chromosome engineering and anchor the cucumber genetic linkage map to its chromosomes,15 sequence-characterized ampli?ed regions (SCAR) and seven simple sequence repeats (SSR) markers from each link-age group of cucumber were used to screen an ordered array of pooled BAC DNA with polymerase chain reaction (PCR). Fifteen mark-ers gave at least two positive clones. As a result,22 BAC clones representing 7 linkage groups of cucumber were identified,which further validated the genome coverage and utility of the library. This BAC library and linkage group specific clones provide essential resources for future research of the cucumber genome. | Yuan Guan Qi Chen Junsong Pan Zheng Li Huanle He Aizhong Wu Rentao Song Run Cai | 2008 | Progress in Natural Science:Materials International2008，18，2： | 7 |
| 4 | Prognostic Value of NT-proBNP in Stable Coronary Artery Disease in Chinese Patients after Percutaneous Coronary Intervention in the Drug-eluting Stent Era显示文摘Objective The predictive value of N-terminal pro-brain natriuretic peptide(NT-proBNP) in patients with stable coronary artery disease(SCAD) in the drug-eluting stent era is not yet clear. We aimed to evaluate the prognostic value of NT-proBNP in SCAD patients after percutaneous coronary intervention(PCI). Methods We examined 4,293 consecutive SCAD patients who underwent PCI between January 2013 and December 2013 in Fuwai Hospital, China. The primary endpoint was all-cause death. NT-proBNP levels were measured before PCI using Elisa kits(Biomedica, Austria). The indication for PCI was based on the degree of coronary stenosis and evidence of ischemia. Results Among 3,187 SCAD patients with NT-proBNP data, after a 2-year follow-up, NT-proBNP levels were predictive for all-cause death in the SCAD population [area under the receiver operating characteristic curve, 0.768; 95% confidence interval(CI), 0.687-0.849; P < 0.001]. At the optimum cutoff point of 732 pg/mL, the sensitivity and specificity of death was 75.0% and 72.3%, respectively. In a multivariable Cox regression model, the death hazard ratio was 6.43(95% CI, 2.99-13.82; P < 0.001) for patients with NT-proBNP levels ≥ 732 pg/mL, compared with < 732 pg/mL. Conclusion NT-proBNP is a strong predictor of 2-year death with SCAD after PCI in the drug-eluting stent era. | ZHAO Xue Yan LI Jian Xin TANG Xiao Fang XU Jing Jing SONG Ying JIANG Lin CHEN Jue SONG Lei GAO Li Jian GAO Zhan QIAO Shu Bin YANG Yue Jin GAO Run Lin XU Bo YUAN Jin Qing | 2018 | Biomedical and Environmental Sciences2018，31，12： | 5 |
| 5 | Human rhinovirus C infection is associated with asthma in children determined by x TAG respiratory viral panel FAST显示文摘Dear Editor,Cumulative evidence supports the role of early-life viral infections,especially respiratory syncytial virus(RSV)and human rhinovirus(HRV),as major antecedents of childhood asthma(Lemanske,2002;Jackson et al.,2008).In this study,the x TAG respiratory viral panel FAST(RVP FAST)assay,a multiplex polymerase chain reaction(PCR)-based method(Arens et al.,2010;BaladaLlasat et al.,2011;Gharabaghi et al.,2011;Selvaraju,2012),was used to investigate the association of infec- | Dongmei Chen Qinwei Song Runan Zhu Yuan Qian Yu Sun Jie Deng Fang Wang Yaxin Ding Run Tian Chuanhe Liu Wenjing Zhu Linqing Zhao | 2017 | Virologica Sinica2017，32，2： | 5 |
| 6 | PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness显示文摘PHYTOCHROME-INTERACTING FACTORS(PIFs)are a group of basic helix-loop-helix transcription factors that can physically interact with photoreceptors,including phytochromes and cryptochromes.It was previously demonstrated that PIFs accumulated in darkness and repressed seedling photomorphogenesis,and that PIFs linked different photosensory and hormonal pathways to control plant growth and development.In this study,we show that PIFs positively regulate the ABA signaling pathway during the seedling stage specifically in darkness.We found that PIFs positively regulate ABI5 transcript and protein levels in darkness in response to exogenous ABA treatment by binding directly to the G-box motifs in the ABI5 promoter.Consistently,PIFs and the G-box motifs in the ABI5 promoter determine ABI5 expression in darkness,and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the dark.Moreover,we discovered that PIFs can physically interact with the ABA receptors PYL8 and PYL9,and that this interaction is not regulated by ABA.Further analyses showed that PYL8 and PYL9 promote PIF4 protein accumulation in the dark and enhance PIF4 binding to the ABI5 promoter,but negatively regulate PIF4-mediated ABI5 activation.Taken together,our data demonstrate that PIFs interact with ABA receptors to orchestrate ABA signaling in darkness by controlling ABI5 expression,providing new insights into the pivotal roles of PIFs as signal integrators in regulating plant growth and development. | Lijuan Qi Shan Liu Cong Li Jingying Fu Yanjun Jing Jinkui Cheng Hong Li Dun Zhang Xiaoji Wang Xiaojing Dong Run Han Bosheng Li Yu Zhang Zhen Li William Terzaghi Chun-Peng Song Rongcheng Lin Zhizhong Gong Jigang Li | 2020 | Molecular Plant2020，13，3： | 3 |
| 7 | Association of CDKN2B-AS1 Polymorphisms with Premature Triple-vessel Coronary Disease and Their Sex Specificity in the Chinese Population显示文摘Objective The aim of this study is to establish whether cyclin-dependent kinase inhibitor 2B antisense RNA 1(CDKN2 B-AS1) gene polymorphisms are associated with premature triple-vessel disease(PTVD). Methods Nine single-nucleotide polymorphisms(rs1063192, rs10757274, rs1333042, rs1333049, rs2285327, rs3217986, rs3217992, rs4977574, and rs9632884) were genotyped in 884 PTVD patients and 907 control subjects(males ≤ 50 years old and females ≤ 60 years old) using the improved multiplex ligase detection reaction method. Results The allele frequencies of rs10757274 G, rs1333049 C, rs4977574 G(all P < 0.001), and rs3217986 G(P = 0.040) were significantly higher in the PTVD group than in the control group, but those of rs1063192 A, rs1333042 G, and rs9632884 C(all P < 0.001) were significantly lower in the former than in the latter. Logistic regression analysis revealed that homozygote AA of rs1333042 is associated with decreased risk for PTVD(OR = 0.42, 95% CI: 0.22-0.82, P = 0.011). In addition, the allele frequencies observed differed between genders. The G allele of rs3217986 was associated with increased risk for PTVD in male patients only(OR = 2.94, 95% CI: 1.27-6.80, P = 0.012) in the dominant model, and no positively mutated allele was found in female patients. Conclusion Polymorphisms of the CDKN2 B-AS1 gene are associated with the incidence of PTVD in the Chinese population. Furthermore, the frequencies of mutated alleles differed between genders. | XU Jing Jing JIANG Lin XU Lian Jun GAO Zhan ZHAO Xue Yan ZHANG Yin SONG Ying LIU Ru SUN Kai GAO Run Lin XU Bo SONG Lei YUAN Jin Qing | 2018 | Biomedical and Environmental Sciences2018，31，11： | 3 |
| 8 | A LexA-based yeast two-hybrid system for studying light- switchable interactions of phytochromes with their interacting partners显示文摘Phytochromes are a family of photoreceptors in plants that perceive the red(R)and far-red(FR)components of their light environment.Phytochromes exist in vivo in two forms,the inactive Pr form and the active Pfr form,that are interconvertible by treatments with R or FR light.It is believed that phytochromes transduce light signals by interacting with their signaling partners.A GAL4-based lightswitchable yeast two-hybrid(Y2H)system was developed two decades ago and has been successfully employed in many studies to determine phytochrome interactions with their signaling components.However,several pairs of interactions between phytochromes and their interactors,such as the phyACOP1 and phyA-TZP interactions,were demonstrated by other assay systems but were not detected by this GAL4 Y2H system.Here,we report a modified LexA Y2H system,in which the LexA DNA-binding domain is fused to the C-terminus of a phytochrome protein.The conformational changes of phytochromes in response to R and FR light are achieved in yeast cells by exogenously supplying phycocyanobilin(PCB)extracted from Spirulina.The well-defined interaction pairs,including phyA-FHY1 and phyB-PIFs,are well reproducible in this system.Moreover,we show that our system is successful in detecting the phyA-COP1 and phyA-TZP interactions.Together,our study provides an alternative Y2H system that is highly sensitive and reproducible for detecting light-switchable interactions of phytochromes with their interacting partners. | Hong Li Xinyan Qin Pengyu Song Run Han Jigang Li | 2021 | aBIOTECH2021，2，2： | 2 |
| 9 | Prion Protein Protects Cancer Cells against Endoplasmic Reticulum Stress Induced Apoptosis显示文摘Unfolded protein response(UPR) is an adaptive reaction for cells to reduce endoplasmic reticulum(ER) stress. In many types of cancers, such as lung cancer and pancreatic cancer, cancer cells may harness ER stress to facilitate their survival and growth. Prion protein(PrP) is a glycosylated cell surface protein that has been shown to be up-regulated in many cancer cells. Since PrP is a protein prone to misfolding, ER stress can result in under-glycosylated PrP, which in turn may activate ER stress. To assess whether ER stress leads to the production of under-glycosylated PrP and whether underglycosylated PrP may contribute to ER stress thus leading to cancer cell apoptosis, we treated different cancer cells with brefeldin A(BFA), thapsigargin(Thps), and tunicamycin(TM). We found that although BFA, Thps, and TM treatment activated UPR, only ATF4 was consistently activated by these reagents, but not other branches of ER stress. However, the canonical PERK-eIF2α-ATF4 did not account for the observed activation of ATF4 in lung cancer cells. In addition, BFA,but neither Thps nor TM, significantly stimulated the expression of cytosolic PrP. Finally, we found that the levels of PrP contributed to anti-apoptosis activity of BFA-induced cancer cell death. Thus, the pathway of BFA-induced persistent ER stress may be targeted for lung and pancreatic cancer treatment. | Zhenxing Gao Min Peng Liang Chen Xiaowen Yang Huan Li Run Shi Guiru Wu Lili Cai Qibin Song Chaoyang Li | 2019 | Virologica Sinica2019，34，2： | 2 |
| 10 | Preferentially released miR-122 from cyclodextrin-based star copolymer nanoparticle enhances hepatoma chemotherapy by apoptosis induction and cytotoxics efflux inhibition显示文摘Chemotherapy,as one of the most commonly used treatment modalities for cancer therapy,provides limited benefits to hepatoma patients,owing to its inefficient delivery as well as the intrinsic chemo-resistance of hepatoma.Bioinformatic analysis identified the therapeutic role of a liver-specific microRNA-miR-122 for enhancing chemo-therapeutic efficacy in hepatoma.Herein,a cyclodextrin-cored star copolymer nanoparticle system(sCDP/DOX/miR-122)is constructed to co-deliver miR-122 with doxorubicin(DOX)for hepatoma therapy.In this nanosystem,miR-122 is condensed by the outer cationic poly(2-(dimethylamino)ethyl methacrylate)chains of sCDP while DOX is accommodated in the inner hydrophobic cyclodextrin cavities,endowing a sequential release manner of miR-122 and DOX.The preferentially released miR-122 not only directly induces cell apoptosis by down regulation of Bcl-w and enhanced p53 activity,but also increases DOX accumulation through inhibiting cytotoxic efflux transporter expression,which realizes synergistic performance on cell inhibition.Moreover,sCDP/DOX/miR-122 displays remarkably increased anti-tumor efficacy in vivo compared to free DOX and sCDP/DOX alone,indicating its great promising in hepatoma therapy. | Qingqing Xiong Yang Bai Run Shi Jian Wang Weiguo Xu Mingming Zhang Tianqiang Song | 2021 | Bioactive Materials2021，6，11： | 2 |
| 11 | Andrographolide?protects against lipopolysaccharide-induced vascular hyporeactivity by suppressing the expression of inducible nitric oxide in periaorticadipose显示文摘 | Hai ML Song YH Run ML | 2013 | J Cardiovasc Pharmacol2013，62，2： | 1 |
| 12 | Microtexture Evolution and Grain Boundary Character Distribution of Interstitial-Free Steels With Moderate Levels of Cold Rolling Reductions显示文摘High-strength interstitial-free steel sheets have very good deep drawability when processed to have{111}recrystallization texture.The microtexture evolution and grain boundary character distribution of interstitial-free steels as a function of moderate levels of cold rolling reductions were investigated by the metallographic microscopy and electron backscatter diffraction technique.The results showed that there was a close relationship between microtexture and grain boundary character distribution for interstitial-free steel,especially the distribution and features of some specific types of coincident-site lattice boundaries.In addition,α-fiber texture was weakened to vanish whileγ-fiber texture strengthened gradually as cold rolling reduction was increased from 20%to 75%for cold rolled and annealed samples.Accordingly,increasing the rolling reduction from 20%to 75%would lead to a significant increase in the proportion ofΣ3boundaries.Also,it was found that the microtexture of 20%cold rolled sample would induce a high frequency ofΣ11grain boundaries,but the microtexture of 75%cold rolled sample would produce moreΣ7andΣ17grain boundaries.It was suggested that texture played a significant role in the formation of grain boundary character distribution. | YANG Jing-jing FAN Li-xia JIA Juan WU Run SONG Xin-li JIANG Li-li | 2013 | Journal of Iron and Steel Research(International)2013，20，12： | 1 |
| 13 | Coexistence of meningioma and other intracranial benign tumors in non-neurofibromatosis type 2 patients:A case report and review of literature显示文摘BACKGROUND The coexistence of meningioma and other intracranial primary benign tumors is rare,especially in non-neurofibromatosis type 2,and there is limited guidance for the management of such patients.Here,we report a series of 5 patients with concomitant meningioma and other intracranial benign tumors,including subependymoma and pituitary adenoma.CASE SUMMARY Five non-neurofibromatosis type 2 patients with simultaneous occurrence of meningioma and other intracranial benign tumors were retrospectively reviewed.The patients had no history of previous irradiation.The clinical features,pre-and postoperative imaging,surgical procedure and pathological findings were extracted from electronic medical records.There were 4 female patients(80%)and 1 male patient(20%).The mean age was 42.8 years(range:29-52 years).The coexisting tumors included subependymoma in 1 case(20%)and pituitary adenoma in 4 cases(80%).The most common clinical symptom was headache(3/5,60%).Four patients(80%)underwent craniotomy.One patient(20%)underwent transsphenoidal surgery followed by transcranial operation.All tumor diagnoses were confirmed by histopathological examination.The mean follow-up was 38.8 mo(range:23-96 mo),and all 5 patients were in a stable condition at the last follow-up.CONCLUSION The simultaneous occurrence of meningioma and other intracranial benign tumors is a rare clinical event.Histological examination is necessary for the accurate diagnosis.Neurosurgeons should select the appropriate surgical strategy according to the clinical features of each patient,which may provide a more favorable prognosis for individual patients. | Tian-Hao Hu Run Wang Hai-Yun Wang Yi-Fu Song Juan-Han Yu Zi-Xun Wang Yu-Zhou Duan Ting Liu Sheng Han | 2022 | World Journal of Clinical Cases2022，10，13： | 1 |
| 14 | Global products of vegetation leaf area and fraction absorbed PAR from year one of MODIS data显示文摘 | R.B Myneni S Hoffman Y Knyazikhin J.L Privette J Glassy Y Tian Y Wang X Song Y Zhang G.R Smith A Lotsch M Friedl J.T Morisette P Votava R.R Nemani S.W Running | 2002 | Remote Sensing of Environment2002，，1： | 1 |
| 15 | Expression of FOXJ1 in hepatocellular carcinoma: Correlation with patients’ prognosis and tumor cell proliferation显示文摘 | Hong‐Wei Chen Xiao‐Dong Huang Hong‐Chen Li Song He Run‐Zhou Ni Cui‐Hua Chen Chen Peng Gang Wu Gui‐Hua Wang Ying‐Ying Wang Yun‐Hong Zhao Yi‐Xin Zhang Ai‐Guo Shen Hui‐Min Wang | 2013 | Mol. Carcinog2013，，8： | 1 |
| 16 | Preparation, structure, and properties of the PTT/SGF composites 显示文摘 | RUN M T SONG H Z HAO Y P | 2009 | Polymer Composites2009，30，6： | 1 |
| 17 | Global products of vegetation leaf area and fraction absorbed PAR from year one of MODIS data显示文摘 | R.B Myneni S Hoffman Y Knyazikhin J.L Privette J Glassy Y Tian Y Wang X Song Y Zhang G.R Smith A Lotsch M Friedl J.T Morisette P Votava R.R Nemani S.W Running | 2002 | Remote Sensing of Environment2002，，1： | 1 |
| 18 | Effect of Different Levels of Copper and Molybdenum Supplements on Serum Lipid Profiles and Antioxidant Status in Cashmere Goats显示文摘 | Wei Zhang Yu Zhang Shi W. Zhang Xian Z. Song Zhi H. Jia Run L. Wang | 2012 | Biological Trace Element Research2012，，3： | 1 |
| 19 | Prognostic Values of Serum Chloride and Sodium Levels in Patients with Three-vessel Disease显示文摘Objective Identification of new risk factors is needed to improve prediction of adverse outcomes in patients with three-vessel disease(TVD). The present study aimed to evaluate the prognostic values of serum chloride and sodium levels in patients with TVD. Methods We used data from a prospective cohort of consecutive patients with angiographically confirmed TVD. The primary endpoint was all-cause death. Cox proportional hazard regression was used to analyze the relationship of serum chloride and sodium levels with long-term outcomes of TVD patients. Results A total of 8,318 participants with available serum chloride and sodium data were included in this analysis. At baseline, patients in the low tertiles group of serum chloride level(≤ 102.0 mmol/L) or serum sodium level(≤ 139.0 mmol/L) had more severe disease conditions. During a median follow-up of 7.5-year, both low serum chloride level and low serum sodium level were found to be associated with an increased risk for mortality in univariate analysis. However, when both parameters were incorporated into a multivariate model, only low serum sodium level remained to be an independent predictor of all-cause death(hazard ratio: 1.16, 95% confidence interval: 1.01-1.34, P = 0.041). Modest but significant improvement of discrimination was observed after incorporating serum sodium level into the Synergy between percutaneous coronary intervention(PCI) with Taxus and Cardiac Surgery score. Conclusion Serum sodium level is more strongly associated with long-term outcomes of TVD patients compared with serum chloride level. Low serum sodium level is an independent risk factor for mortality, but only provides modest prognostic information beyond an established risk model. | ZHANG Ce WANG Dong HOU Bing Jie JIANG Lin XU Lian Jun TIAN Jian ZHAO Yan Yan ZHAO Xue Yan FENG Xin Xing ZHANG Yin SUN Kai XU Bo ZHAO Wei HUI Ru Tai GAO Run Lin LOU Hui Ling YUAN Jin Qing SONG Lei | 2019 | Biomedical and Environmental Sciences2019，32，4： | 1 |
| 20 | Melting,crystallization behaviors, and nonisothermal crystallization kinetics of PET/PTT/PBT ternary blends 显示文摘 | Run M T Song A J Wang Y J | 2007 | Journal of Applied Polymer Science2007，104，5： | 1 |
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