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| 1 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 2 | Cloning the Structure Genes and Expression the N Gene of Porcine Epidemic Diarrhea Virus DX显示文摘The structure genes spike(S) ,nucleocapsid(N) ,membrane(M) ,small membrane(sM) of a porcine epidemic diarrhea virus(PEDV) strain DX isolated in Gansu province,North-west of China,were cloned,sequenced and compared with published sequences of PEDV strains. The nucleotide sequences encoding the entire S,sM,M and N genes open reading frame(ORF) of DX were 4 152,231,681 and 1 326 bases long respectively. There were transcription regulatory sequences(TRSs) upstream of the initiator ATG of the S,N and M genes. The amino acids sequences of S,M and N contained 30,3 and 7 potential asparagine(N) -linked glycosylation sites. Homologous analysis and phylogenetic trees showed that DX had the closest relationship with strains LJB/06,JS-2004-2Z and CH/HLJH/06 that were also isolated from China and indicated the prevalence of some PEDV isolates in China were widespread since the JS-2004-2Z strain originated from the south of the China,and LJB/06 and CH/HLJH/06 were isolated from northeast China. The N gene was cloned using two primers which contained Nco I and BamH I restriction enzyme sites and subcloned into expression vector pET30a. The recombinant plasmid was then transformed into E.coli Rossta. SDS-PAGE showed there was a protein of about 55kDa as expected and Western blot indicated the N protein had biological activity. | Jian-qiang LI Ji-xing LIU Xi LAN Jie CHENG Run WU Zhong-Zi LOU Xiang-ping YIN Xue-rui LI Bao-yu LI Bin YANG Zhi-yong LI | 2009 | Virologica Sinica2009，24，3： | 4 |
| 3 | PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness显示文摘PHYTOCHROME-INTERACTING FACTORS(PIFs)are a group of basic helix-loop-helix transcription factors that can physically interact with photoreceptors,including phytochromes and cryptochromes.It was previously demonstrated that PIFs accumulated in darkness and repressed seedling photomorphogenesis,and that PIFs linked different photosensory and hormonal pathways to control plant growth and development.In this study,we show that PIFs positively regulate the ABA signaling pathway during the seedling stage specifically in darkness.We found that PIFs positively regulate ABI5 transcript and protein levels in darkness in response to exogenous ABA treatment by binding directly to the G-box motifs in the ABI5 promoter.Consistently,PIFs and the G-box motifs in the ABI5 promoter determine ABI5 expression in darkness,and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the dark.Moreover,we discovered that PIFs can physically interact with the ABA receptors PYL8 and PYL9,and that this interaction is not regulated by ABA.Further analyses showed that PYL8 and PYL9 promote PIF4 protein accumulation in the dark and enhance PIF4 binding to the ABI5 promoter,but negatively regulate PIF4-mediated ABI5 activation.Taken together,our data demonstrate that PIFs interact with ABA receptors to orchestrate ABA signaling in darkness by controlling ABI5 expression,providing new insights into the pivotal roles of PIFs as signal integrators in regulating plant growth and development. | Lijuan Qi Shan Liu Cong Li Jingying Fu Yanjun Jing Jinkui Cheng Hong Li Dun Zhang Xiaoji Wang Xiaojing Dong Run Han Bosheng Li Yu Zhang Zhen Li William Terzaghi Chun-Peng Song Rongcheng Lin Zhizhong Gong Jigang Li | 2020 | Molecular Plant2020，13，3： | 3 |
| 4 | Occurrence of Streptococcus dysgalactiae subsp, equisimilis in Masked Palm Civet (Paguma larvata)显示文摘 | Run Cheng Li Chao Ting Xiao Xing Qian | 2012 | 11(12):2020-20232012，11，12： | 1 |
| 5 | Study on Reduction of β-Aromatic Amino Substituted Triazolyl Ketene Compounds显示文摘A series of b-aromatic amino substituted triazolyl ketenes were reduced at different conditions to enols and saturated alcohols, respectively. The preliminary biological tests showed that some of them exhibit good fungicidal activities. | Yang Zhou LI, Jun Ran CHENG*, Qing Min WANG, Xiang Yun GUO, Run Qiu HUANG Institute and State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin 300071 | 2003 | Chinese Chemical Letters2003，14，5： | 1 |
| 6 | Occurrence of Streptococcus dysgalactiae subsp, equisimilis in Masked Palm Civet (Paguma larvata)显示文摘 | Run Cheng Li Chao Ting Xiao Xing Qian | 2012 | Journal of Animal and Veteri- nary Advances2012，11，12： | 1 |
| 7 | Management experience of care workers during epidemic prevention and control of COVID-19显示文摘Since the rapid spread of COVID‑19,our hospital has actively implemented the idea of“preventing the coronavirus from entering.”We paid close attention to details,rapidly carried out personnel and material management at all levels,and strived to do a good job in the prevention and control of the epidemic,so as to achieve zero infection.Patients’carers are an important part of quality care.Lacking of protection knowledge of infectious diseases would increase the risk of nosocomial infection.Therefore,our nursing department set up a team of epidemic prevention and control management.The team made the revision on contingency plans and care management regulations during the outbreak of COVID‑19,strengthened epidemic prevention and control knowledge training,carried out the supervision inspection work,and paid attention to humanized management measures to provide an important guarantee for victory over the COVID‑19. | Na CHENG Run‑Xi TIAN Nan TONG Qing GONG Jia‑Yue LI Bao‑Jing WU De‑Yu KONG | 2020 | Journal of Integrative Nursing2020，2，2： | 1 |
| 8 | Advances in nuclear detection and readout techniques显示文摘“A Craftsman Must Sharpen His Tools to Do His Job,”said Confucius.Nuclear detection and readout techniques are the foundation of particle physics,nuclear physics,and particle astrophysics to reveal the nature of the universe.Also,they are being increasingly used in other disciplines like nuclear power generation,life sciences,environmental sciences,medical sciences,etc.The article reviews the short history,recent development,and trend of nuclear detection and readout techniques,covering Semiconductor Detector,Gaseous Detector,Scintillation Detector,Cherenkov Detector,Transition Radiation Detector,and Readout Techniques.By explaining the principle and using examples,we hope to help the interested reader underst and this research field and bring exciting information to the community. | Rui He Xiao‑Yang Niu Yi Wang Hong‑Wei Liang Hong‑Bang Liu Ye Tian Hong‑Lin Zhang Chao‑Jie Zou Zhi‑Yi Liu Yun‑Long Zhang Hai‑Bo Yang Ju Huang Hong‑Kai Wang Wei‑Jia Han Bei Cao Gang Chen Cong Dai Li‑Min Duan Rui‑Rui Fan Fang‑Fa Fu Jian‑Hua Guo Dong Han Wei Jiang Xian‑Qin Li Xin Li Zhuo‑Dai Li Yu‑Tie Liang Shun Liao De‑Xu Lin Cheng‑Ming Liu Guo‑Rui Liu Jun‑Tao Liu Ze Long Meng‑Chen Niu Hao Qiu Hu Ran Xiang‑Ming Sun Bo‑Tan Wang Jia Wang Jin‑Xiang Wang Qi‑Lin Wang Yong‑Sheng Wang Xiao‑Chuan Xia Hao‑Qing Xie He‑Run Yang Hong Yin Hong Yuan Chun‑Hui Zhang Rui‑Guang Zhao Ran Zheng Cheng‑Xin Zhao | 2023 | Nuclear Science and Techniques2023，34，12： | 0 |
| 9 | A spin-less particle on a rotating curved surface in Minkowski space显示文摘In Minkowski space M,we derive the effective Schrodinger equation describing a spin-less particle confined to a rotating curved surface S.Using the thin-layer quantization formalism to constrain the particle on we obtain the relativity-corrected geometric potential V_(g)’,and a novel effective potential V(g) related to both the Gaussian curvature and the geodesic curvature of the rotating surface.The Coriolis effect and the centrifugal potential also appear in the equation.Subsequently,we apply the surface Schrodinger equation to a rotating cylinder,sphere and toms surfaces,in which we find that the interplays between the rotation and surface geometry can contribute to the energy spectrum based on the potentials they offer. | Run Cheng Li Wang Hao Zhao Cui-Bai Luo Yong-Long Wang Jun Wang | 2021 | Communications in Theoretical Physics2021，73，12： | 0 |
| 10 | Efficient Isomerization for Preparing thans-2,2-Dimethyl-3-(2,2-dichloroethenyl) cyclopropanecarbonyl Chloride显示文摘Trans-2,2-dimethyl-3-(2,2-dichloroethenyl)cyclopropanecarbonyl chloride, which is animportant intermediate for insecticides, is obtained by isomerization of the cis/trans-mixture with4- (N,N-dimethylamino) pyridine (DMAP) in the presence or absence of halogen compounds ingood yield. | Jun An MA Run Qiu HUANG Jun Ran CHENG Rui Lian SHAO Zai Guo LI(Institute and State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin300071) | 1999 | Chinese Chemical Letters1999，10，2： | 0 |
| 11 | Structure and Properties of Sol-Gel Derived CuNiMnZrO2 Catalysts for CO Hydrogenation显示文摘Two CuNiMnZrO2 catalysts (crystallized or non-crystallized) were prepared by co-hydrolyzing zirconium n-propoxide with Cu(NO3)2, Mn(NO3)2 and Ni(NO3)2 in an ethanol solution in the presence of diglycol. The physical and chemical properties of the catalysts were characterized using BET, XRD,TEM, XPS and CO-DRIFT techniques. It was found that the non-crystallized CuNiMnZrO2 catalyst demonstrated highly dispersed active phases and high activity for CO adsorption, which resulted in good performance for synthesis of higher alcohols in CO hydrogenation compared to crystallized CuNiMnZrO2. | Zhongyi Ma, Cheng Yang, Run Xu. Wei Wei, Wenhuai Li, Yuhan SunState Key Laboratory of Coal Conversion, Institute uf Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, China | 2003 | Journal of Natural Gas Chemistry2003，12，2： | 0 |
| 12 | Research on the Critical Buckling Pressure of Bucket Foundations with Inner Compartments for Offshore Wind Turbines显示文摘In the process of suction penetration of bucket foundations with inner compartments for offshore wind turbines,most researches focus on soil seepage failure and soil plugs,while the buckling of foundations is rarely investigated.Therefore,theoretical calculation methods for critical buckling pressures of the skirt and bulkheads of the bucket foundation are first presented according to the stability theory of a cylindrical shell and the small deflection theory of a thin plate,respectively.Furthermore,two types of models with and without considering the skirt-soil interaction are developed for the calculation of critical buckling pressure of the bucket foundation.Taking a practical project as an example,theoretical and numerical methods are used to obtain the critical buckling pressures of a bucket foundation.In this work,the theoretical method and the finite element model considering the skirt-soil interaction for calculating the critical buckling pressure of bucket foundations are firstly proposed.The results can help to optimize the design process of offshore wind turbine foundations and improve the safety of offshore wind power systems. | SUN Guodong LIU Run LI Qingxin HUANG Guoyu ZHOU Zhenbo ZHANG Cheng | 2023 | Journal of Ocean University of China2023，22，2： | 0 |
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