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| 1 | Rapid Detection of Wheat Blast Pathogen Magnaporthe oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Technology显示文摘Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and Bangladesh.Since MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is ineffective.To develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)pathotype.Using polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and Bangladesh.To test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR machine.Following this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 sequences.The activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)activity.We then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat plants.This novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field. | Houxiang Kang Ye Peng Kangyu Hua Yufei Deng Maria Bellizzi Dipali Rani Gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson Yilin Zhou Xueping Zhou Md Tofazzal Islam Guo-Liang Wang | 2021 | Engineering2021，7，9： | 4 |
| 2 | Development of soft sensor for neural network based control of distillation column显示文摘 | RANI A SINGH V GUPTA J R P | 2013 | ISA Transactions2013，52，3： | 1 |
| 3 | A Review and Analysis of Localization Techniques in Underwater Wireless Sensor Networks显示文摘In recent years,there has been a rapid growth in Underwater Wireless Sensor Networks(UWSNs).The focus of research in this area is now on solving the problems associated with large-scale UWSN.One of the major issues in such a network is the localization of underwater nodes.Localization is required for tracking objects and detecting the target.It is also considered tagging of data where sensed contents are not found of any use without localization.This is useless for application until the position of sensed content is confirmed.This article’s major goal is to review and analyze underwater node localization to solve the localization issues in UWSN.The present paper describes various existing localization schemes and broadly categorizes these schemes as Centralized and Distributed localization schemes underwater.Also,a detailed subdivision of these localization schemes is given.Further,these localization schemes are compared from different perspectives.The detailed analysis of these schemes in terms of certain performance metrics has been discussed in this paper.At the end,the paper addresses several future directions for potential research in improving localization problems of UWSN. | Seema Rani Anju Anupma Sangwan Krishna Kumar Kashif Nisar Tariq Rahim Soomro Ag.Asri Ag.Ibrahim Manoj Gupta Laxmi Chandand Sadiq Ali Khan | 2023 | Computers, Materials & Continua2023，，6： | 1 |
| 4 | Microbial keratinases and their prospective applications: an overview显示文摘 | Rani Gupta Priya Ramnani | 2006 | Applied Microbiology and Biotechnology2006，，1： | 1 |
| 5 | Purification and characterization of an oxidation-stable, thiol-dependent serine alkaline protease from Bacillus mojarensis 显示文摘 | Qasim Khalil Beg Rani Gupta | 2003 | Enzyme and Microbial Technology2003，32，： | 1 |
| 6 | Isolation of a library of target-sites for sequence specific DNA binding proteins from chick embryonic heart: A potential tool for identifying novel transcriptional regulators involved in embryonic development显示文摘 | Sindhu K V Rani V Gupta M K | 2004 | Biochemical and Biophysical Research Communications2004，323，3： | 1 |
| 7 | A lattice dynamical investigation of the raman and the infrared frequencies of the cubic A2Sn207 pyrochlores 显示文摘 | GUPTA H C BROWN S RANI N | 2001 | International Journal of Inorganic Materials2001，3，7： | 1 |
| 8 | Albuminuria and Diabetic Retinopathy in Type 2 Diabetes Mellitus Sankara Nethralaya Diabetic Retinopathy Epidemiology And Molecular Genetic Study (SN- DREAMS, report 12)显示文摘 | Rani PK Raman R Gupta A | 2011 | Diabetol Metab Syndr2011，3，1： | 1 |
| 9 | A Novel Alkaline Lipase from Burkholdderia Cepacia for Detergent Formulation 显示文摘 | Pooja Rathi Saxena R K Rani Gupta | 2001 | Process Biochem2001，37，： | 1 |
| 10 | A novel alkaline lipase from Barkholderia Cepacia for detergen显示文摘 | Pooja Rathi R K Saxena Rani Gupta | 2001 | Formulation Process Biochemistry2001，1，： | 1 |
| 11 | Laparoscopic nephroplication and nephropexy as an adjunct to pyeloplasty in UPJO with giant hydronephrosis显示文摘 | Lokesh Jindal Anup Kumar Gupta Faiz Mumtaz Rani Sunder A. K. Hemal | 2006 | International Urology and Nephrology (-)2006，，3： | 1 |
| 12 | Microbial keratinases and their prospective applications: an overview显示文摘 | Rani Gupta Priya Ramnani | 2006 | Applied Microbiology and Biotechnology2006，，1： | 1 |
| 13 | Concurrent sorption of Zn( Ⅱ ), Cu( Ⅱ )and Co( Ⅱ ) by Oscillatoria angustissima as a function of pH in binary and ternary metal solutions显示文摘 | Harapriya Mohapatra Rani Gupta | 2005 | bioresource technology2005，96，： | 1 |
| 14 | Optimization of medium composition for keratinase production on feather by Bacillus licheniformis RG1 using statistical methods involving response surface methodology显示文摘 | Ramnani Priya Gupta Rani | 2004 | Biotechnol Appl Biochem2004，40，： | 1 |
| 15 | Speciation of heavy metals in water and sediments of an urban lake system显示文摘 | Gupta B Kumar R Rani M | 2013 | Journal of Environmental Science and Health Part a-Toxic/Hazardous Substances & Environmental Engineering2013，48，10： | 1 |
| 16 | Degradation of thiram in water, soil and plants: A study by high-performance liquid chromatography显示文摘 | Gupta B Rani M Kumar R | 2012 | Biomed Chromatogr2012，26，1： | 1 |
| 17 | Zn^2+ biosorption by Oscillatoria anguistissima显示文摘 | Prema Ahuja Rani Gupta R K Saxena | 1999 | Process Biochemistry1999，34，： | 1 |
| 18 | Albuminuria and diabetic retinopathy in type 2 diabetes mellitus sankara nethralaya diabetic retinopathy epidemiology and molecular genetic study (SN-DRE-AMS,report 12)显示文摘 | Rani PK Raman R Gupta A | | 0，，： | 1 |
| 19 | Fermentation Waste of Aspergillus Terreus: a Potential Copper Biosorbent显示文摘 | SAXENA R K RANI Gupta | 2000 | World Journal of Microbiology & Biotechnology2000，18，： | 1 |
| 20 | Microbial a-amylases: a biotechnological perspective显示文摘 | Rani Gupta Paresh Gigras Harapriya Mohapatra Vineet Kum Gos- wami Bhavna Chauhan | 2003 | Process Biochenistry2003，38，11： | 1 |
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