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| 1 | Attenuation of dextran sodium sulphate induced colitis in matrix metalloproteinase-9 deficient mice显示文摘AIM: To study whether matrix metalloproteinase-9 (MMP-9) is a key factor in epithelial damage in the dextran sodium sulphate (DSS) model of colitis in mice.METHODS: MMP-9-deficient and wild-type (wt) mice were given 5% DSS in drinking water for 5 d followed by recovery up to 7 d. On d 5 and 12 after induction of colitis, gelatinases, MMP-2 and MMP-9, were measured in homogenates of colonic tissue by zymography and Western blot, whereas tissue inhibitor of metalloproteinases (TIMPs) were measured by reverse zymography. The gelatinolytic activity was also determined in supernatants of polymorphonuclear leukocytes (PMN) isolated from mice blood. Moreover, intestinal epithelial cells were stimulated with TNF-α to study whether these cells were able to produce MMPs. Finally, colonic mucosal lesions were measured by microscopic examination. RESULTS: On d 5 of colitis, the activity of MMP-9 was increased in homogenates of colonic tissues (0.24 ± 0.1 vs 21.3 ± 6.4, P < 0.05) and PMN from peripheral blood in wt (0.5 ± 0.1 vs 10.4 ± 0.7, P < 0.05), but not in MMP-9-deficient animals. The MMP-9 activity was also up-regulated by TNF-α in epithelial intestinal cells (2.5 ± 0.5 vs 14.7 ± 3.0, P < 0.05). Although colitis also led to increase of TIMP-1 activity, the MMP-9/TIMP-1 balance remained elevated. Finally, in the MMP-9-deficient colitic mice both the extent and severity of intestinal epithelialinjury were significantly attenuated when compared with wt mice. CONCLUSION: We conclude that DSS induced colitis is markedly attenuated in animals lacking MMP-9. This suggests that intestinal injury induced by DSS is modu-lated by MMP-9 and that inhibition of this gelatinase may reduce inflammation. | Alfredo Santana Carlos Medina Maria Cristina Paz-Cabrera Federico Díaz-Gonzalez Esther Farré Antonio Salas Marek W Radomski Enrique Quintero | 2006 | World Journal of Gastroenterology2006，12，40： | 9 |
| 2 | Endoscopic submucosal dissection training with pig models in a Western country显示文摘AIM:To test a strategy for endoscopic submucosal dissection(ESD) training in animal models designed to overcome the initial learning curve.METHODS:ESD was attempted in ex vivo and in vivo pig models.Thirty ESD procedures were attempted in the esophagus(n=9) or the stomach(n=21).The ex vivo model was used until initial competence was achieved.In the in vivo model,several ESD procedures were performed in up to 3 sessions.The following variables were analyzed:specimen size,complete and en bloc resection rate,time for circumferential incision,time for submucosal dissection,total ESD duration,and complications.RESULTS:Complete resection was achieved in 28 cases(en bloc 27);2 could not be completed(one perforation,one technical diff iculty).The mean ± SD time for circumferential incision was 36.2±16.8 min(range:8-87 min),and the mean±SD time for submucosal dissection was 45.1±35.7 min(range:9-196 min).The mean±SD size of the resected specimens was 45.2±17.8 mm.The mean±SD total resection time was signif icantly increased for the gastric cases performed in the f irst half of the study(n=13) than in the second half(n=8)(98.9±62.4 min vs 61.7±17.6 min,P=0.04),although the specimen size did not differ.CONCLUSION:Training in animal models could help endoscopists overcome the learning curve before starting ESD in humans. | Adolfo Parra-Blanco María Rosa Arnau David Nicolás-Pérez Antonio Z Gimeno-García Nicolás González Juan A Díaz-Acosta Alejandro Jiménez Enrique Quintero | 2010 | World Journal of Gastroenterology2010，16，23： | 9 |
| 3 | Loss of Halophytism by Interference with SOS1 Expression1[W][OA]显示文摘 | Oh Dong-Ha Leidi Eduardo Zhang Quan Hwang Sung-Min Li Youzhi Quintero Francisco J Jiang Xingyu D’Urzo Matilde Paino Lee Sang Yeol Zhao Yanxiu Bahk Jeong Dong Bressan Ray A Yun Dae-Jin Pardo José M Bohnert Hans J | 2009 | Plant Physiology2009，，1： | 1 |
| 4 | Open reduction and internal fixation of fractures of the radial head显示文摘 | Ring D Quintero J Jupiter JB | 2002 | J Bone Joint Surg Am2002，84，1： | 1 |
| 5 | Household social determinants of ascariasis and trichuriasis in North Central Venezuela 显示文摘 | Quintero K DUrhn C Duri D | 2012 | Int Health2012，4，2： | 1 |
| 6 | An efficient temary complex of acetazolamide with HP-ss-CD and TEA for topical ocular administration 显示文摘 | PALMA S D TARTARA L I QUINTEROS D | 2009 | J Control Release2009，138，1： | 1 |
| 7 | Atrophic united diaphyseal frac- tures of the humeruswith a bony defect: treatment by wave plat osteosyn- thesis 显示文摘 | Ring D Jupiter JB Quintero J | 2000 | J Bone Joint Surg Br2000，82，6： | 1 |
| 8 | Open reduction and internal fixation of fractures of the radial head 显示文摘 | Ring D Quintero J Jupiter JB | 2002 | J Bone Joint Surg (Am)2002，84，10： | 1 |
| 9 | Open reduction and internal fixation of the fractures of the radial head显示文摘 | Ring D Quintero J Jupiter JB | 2002 | J Bone Joint Surg(Am)2002，84，： | 1 |
| 10 | Atrophic ununited diaphysealfractures of the humerus with a bony defect treatment by wave-platos-teosynthesis显示文摘 | Ring D Jupiter JB Quintero J | 2000 | J Bone Joint Surg Br2000，6，： | 1 |
| 11 | Open reduction and internal fixation of the fractures of the radial head 显示文摘 | Ring D Quintero J Jupiter JB | 2002 | J Bone Joint Surg (Am)2002，84，10： | 1 |
| 12 | Bone grafting and guide bone regenerantion for immediate dental implants in human显示文摘 | Gher ME Quintero G Assad D | 1994 | J Periodentol1994，65，： | 1 |
| 13 | Open re- duction and internal fixation of fractures ofthe radial head 显示文摘 | Ring D Quintero J Jupiter JB | 2002 | J Bone Joint Surg(Am)2002，84，： | 1 |
| 14 | Open reduction and in-ternal fixation of the firactures of the radial head显示文摘 | Ring D Quintero J Jupiter JB | | 0，，10： | 1 |
| 15 | Open reduction and internal fixation of fractures of the radial head显示文摘 | Rink D Quintero J Jupiter JB | 2002 | J Bone Joint Surg Am2002，84，10： | 1 |
| 16 | A collaborative learning environment architecture supporting quality of services 显示文摘 | QUINTERO A PIERRE S’TASSY D | 2006 | Advanced Technology for Learning2006，，： | 1 |
| 17 | A site-specific recombinase (RinQ) is required to exert incompatibility towards the symbiotic plasmid of Rhizobium etli显示文摘 | QUINTERO V MIGUEL A CEVALLOS D G | 2002 | Mol Microbiol2002，46，： | 1 |
| 18 | Antinociceptive activity of Syzygium jambos leaves extract on rats 显示文摘 | Avila-Pena D Pena N Quintero L | 2007 | J Ethnopharmacol2007，112，2： | 1 |
| 19 | Matrix metalloproteinase-8 inactivates macrophage inflammatory protein-1α to reduce acute lung inflammation and injury in mice显示文摘 | Quintero P A Knolle M D Cala L F | 2010 | J Immunol2010，184，： | 1 |
| 20 | Atrophic Ununited Diaphyseal Fractures of the Humerus with a Bony Defect Treatment by Wave-plate Osteosynthesis显示文摘 | Ring D Jupiter J B Quintero J | 2000 | J Bone Joint Surg Br2000，82，6： | 1 |
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