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| 1 | H pylori (CagA) and Epstein-Barr virus infection in gastric carcinomas:Correlation with p53 mutation and c-Myc,Bcl-2 and Bax expression显示文摘AIM: To investigate the interrelationship between H pylori and Epstein-Barr virus (EBV) infection in the gastric carcinogenesis having in focus the p53 mutation and the c-Myc, Bcl-2 and Bax expression. METHODS: seventy-one gastric carcinoma tissues were assessed by polymerase chain reaction (PCR) for H pylori and in situ hybridization for EBV. c-Myc, Bcl-2 and Bax expression were detected by immunohistochemistry and single-stranded conformational polymorphism (SSCP) for p53 mutation. RESULTS: The positivity rates for H pylori and EBV were 94.4% and 8.45%, respectively. The majority of the cases displayed only the H pylori presence. All EBV positive cases were also H pylori positive. None infectious agent was observed in 5.55% of the cases. The intestinal type tumor was more frequent in the co-infected and non-infected groups. The female predominated in the non-infected group showing statistical significance (70.4% vs 29.6%, P=0.039). The Bcl-2 was only detected in the group exclusively infected by H pylori. However, c-Myc and Bax were detected in the three groups but with a low frequency in the co-infected group. Mutation of p53 was present in all groups, with the highest frequencies in the H pylori positive groups. CONCLUSION: The frequency of H pylori infection in gastric carcinomas was high. The presented data indicated that gastric carcinogenesis has different pathways depending of the presence of the two investigated infectious agents, suggesting a possible involvement of H pylori with apoptotic process. The low expression of c-Myc and Bax in the EBV-positive groups suggests that EBV may inhibit the expression of these proteins. Nevertheless, p53 mutation shows to be a relevant alteration, independent of both infectious agents. | Valeska Portela Lima Marcos Antonio Pereira de Lima Angela Rosa André Márcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Sílvia Helena Barem Rabenhorst | 2008 | World Journal of Gastroenterology2008，14，6： | 17 |
| 2 | Helicobacter pylori and EBV in gastric carcinomas:Methylation status and microsatellite instability显示文摘AIM:To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA+ and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H. pylori and genotyping were performed by PCR, using specifi c primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the χ2 or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA+ in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H. pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. | Adriana Camargo Ferrasi Nídia Alice Pinheiro Silvia Helena Barem Rabenhorst Otávia Luisa Caballero Maria Aparecida Marchesan Rodrigues Fabrício de Carvalho Celso Vieira de Souza Leite Marcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Maria Inês de Moura Campos Pardini | 2010 | World Journal of Gastroenterology2010，16，3： | 6 |
| 3 | Induced pluripotent stem cells throughout the animal kingdom:Availability and applications显示文摘Up until the mid 2000s, the capacity to generate every cell of an organism was exclusive to embryonic stem cells. In 2006, researchers Takahashi and Yamanaka developed an alternative method of generating embryonic-like stem cells from adult cells, which they coined induced pluripotent stem cells (iPSCs). Such iPSCs possess most of the advantages of embryonic stem cells without the ethical stigma associated with derivation of the latter. The possibility of generating “custom-made” pluripotent cells, ideal for patient-specific disease models, alongside their possible applications in regenerative medicine and reproduction, has drawn a lot of attention to the field with numbers of iPSC studies published growing exponentially. IPSCs have now been generated for a wide variety of species, including but not limited to, mouse, human, primate, wild felines, bovines, equines, birds and rodents, some of which still lack well-established embryonic stem cell lines. The paucity of robust characterization of some of these iPSC lines as well as the residual expression of transgenes involved in the reprogramming process still hampers the use of such cells in species preservation or medical research, underscoring the requirement for further investigations. Here, we provide an extensive overview of iPSC generated from a broad range of animal species including their potential applications and limitations. | Lais Vicari de Figueiredo Pessoa Fabiana Fernandes Bressan Kristine Karla Freude | 2019 | World Journal of Stem Cells2019，11，8： | 4 |
| 4 | Proton nuclear magnetic resonance-based metabonomic models for non-invasive diagnosis of liver fibrosis in chronic hepatitis C:Optimizing the classification of intermediate fibrosis显示文摘AIM To develop metabonomic models(MMs), using 1 H nuclear magnetic resonance(NMR) spectra of serum, to predict significant liver fibrosis(SF: Metavir ≥ F2), advanced liver fibrosis(AF: METAVIR ≥ F3) and cirrhosis(C: METAVIR = F4 or clinical cirrhosis) in chronic hepatitis C(CHC) patients. Additionally, to compare the accuracy of the MMs with the aspartate aminotransferase to platelet ratio index(APRI) and fibrosis index based on four factors(FIB-4). METHODS Sixty-nine patients who had undergone biopsy in the previous 12 mo or had clinical cirrhosis were included. The presence of any other liver disease was a criterion for exclusion. The MMs, constructed using partial least squares discriminant analysis and linear discriminant analysis formalisms, were tested by cross-validation, considering SF, AF and C. RESULTS Results showed that forty-two patients(61%) presented SF, 28(40%) AF and 18(26%) C. The MMs showed sensitivity and specificity of 97.6% and 92.6% to predict SF; 96.4% and 95.1% to predict AF; and 100% and 98.0% to predict C. Besides that, the MMs correctly classified all 27(39.7%) and 25(38.8%) patients with intermediate values of APRI and FIB-4, respectively. CONCLUSION The metabonomic strategy performed excellently in predicting significant and advanced liver fibrosis in CHC patients, including those in the gray zone of APRI and FIB-4, which may contribute to reducing the need for these patients to undergo liver biopsy. | Andrea Dória Batista Carlos Jonnatan Pimentel Barros Tássia Brena Barroso Carneiro Costa Michele Maria Goncalves de Godoy Ronaldo Dionísio Silva Joelma Carvalho Santos Mariana Montenegro de Melo Lira Norma ThoméJucá Edmundo Pessoa de Almeida Lopes Ricardo Oliveira Silva | 2018 | World Journal of Hepatology2018，10，1： | 2 |
| 5 | Balanced propofol sedation administered by nonanesthesiologists:The first Italian experience显示文摘AIM:To assess the efficacy and safety of a balanced approach using midazolam in combination with propofol,administered by nonanesthesiologists,in a large series of diagnostic colonoscopies.METHODS:Consecutive patients undergoing diagnostic colonoscopy were sedated with a single dose of midazolam(0.05 mg/kg)and lowdose propofol(starter bolus of 0.5 mg/kg and repeated boluses of 10 to 20 mg).Induction time and deepest level of sedation,adverse and serious adverse events,as well as recovery times,were prospectively assessed.Cecal intubation and adenoma detection rates were also collected.RESULTS:Overall,1593 eligible patients were included.The median dose of propofol administered was 70 mg(range:40120 mg),and the median dose of midazolam was 2.3 mg(range:24 mg).Median induction time of sedation was 3 min(range:14 min),and median recovery time was 23 min(range:1040 min).A moderate level of sedation was achieved in 1561(98%) patients,whilst a deep sedation occurred in 32(2%) cases.Transient oxygen desaturation requiring further oxygen supplementation occurred in 8(0.46%;95% CI:0.2%0.8%)patients.No serious adverse event was observed.Cecal intubation and adenoma detection rates were 93.5%and 23.4%(27.8%for male and 18.5%for female,subjects),respectively.CONCLUSION:A balanced sedation protocol provided a minimalization of the dose of propofol needed to target a moderate sedation for colonoscopy,resulting in a high safety profile for nonanesthesiologist propofol sedation. | Alessandro Repici Nico Pagano Cesare Hassan Alessandra Carlino Giacomo Rando Giuseppe Strangio Fabio Romeo Angelo Zullo Elisa Ferrara Eva Vitetta Daniel de Paula Pessoa Ferreira Silvio Danese Massimo Arosio Alberto Malesci | 2011 | World Journal of Gastroenterology2011，17，33： | 2 |
| 6 | Antifungal activity and antidiarrheal activity via antimotility mechanisms of (-)-fenchone in experimental models显示文摘BACKGROUND(-)-Fenchone is a bicyclic monoterpene present in essential oils of plant species,such as Foeniculum vulgare and Peumus boldus,used to treatment of gastrointestinal diseases.Pharmacological studies report its anti-inflammatory,antioxidant,and antinociceptive activity.AIM To investigate antidiarrheal activity related to gastrointestinal motility,intestinal secretion and antimicrobial activity.METHODS A castor oil-induced diarrhea model was used to evaluate antidiarrheal activity.Intestinal transit and gastric emptying protocols were used to assess a possible antimotility effect.Muscarinic receptors,presynaptic α2-adrenergic and tissue adrenergic receptors,KATP channels,nitric oxide were investigated to uncover antimotility mechanisms of action and castor oil-induced enteropooling to elucidate antisecretory mechanisms.The antimicrobial activity was evaluated in the minimum inhibitory concentration model,the fractional inhibitory concentration index using the(-)-fenchone association method with standard antifungal agents.RESULTS(-)-Fenchone(75,150 and 300 mg/kg)showed antidiarrheal activity,with a significant decrease in the evacuation index.This activity is possibly related to a percentage of reduced intestinal transit(75,150 and 300 mg/kg).The antimotility effect of(-)-fenchone decreased in the presence of pilocarpine,yohimbine,propranolol,L-NG-nitroarginine methyl ester or glibenclamide.In the enteropooling model,no reduction in intestinal fluid weight was observed.(-)-Fenchone did not show antibacterial activity;on the other hand,inhibits the growth of strains of fungi with a minimum fungicide concentration of 32μg/mL.However,when it was associated with amphotericin B,no synergism was observed.CONCLUSION The antidiarrheal effect of(-)-fenchone in this study involves antimotility effect and not involve antisecretory mechanisms.(-)-Fenchone presents antifungal activity;however,it did not show antibacterial activity. | Michelle Liz de Souza Pessoa Leiliane Macena Oliveira Silva Maria Elaine Cristina Araruna Catarina Alves de Lima Serafim Edvaldo Balbino Alves Junior Alessa Oliveira Silva Matheus Marley Bezerra Pessoa Hermes Diniz Neto Edeltrudes de Oliveira Lima Leonia Maria Batista | 2020 | World Journal of Gastroenterology2020，26，43： | 2 |
| 7 | Safety of cold polypectomy for < 10 mm polyps at colonoscopy: a prospective multicenter study显示文摘 | A. Repici C. Hassan E. Vitetta E. Ferrara G. Manes G. Gullotti A. Princiotta P. Dulbecco N. Gaffuri E. Bettoni N. Pagano G. Rando G. Strangio A. Carlino F. Romeo D. de Paula Pessoa Ferreira A. Zullo L. Ridola A. Malesci | 2012 | Endoscopy2012，，01： | 2 |
| 8 | Effective and simple recovery of 1,3-propanediol from a fermented medium by liquid–liquid extraction system with ethanol and K_3PO_4显示文摘1,3-Propanediol,traditionally obtained from fossils,has numerous industrial applications,including use in the production of high performance polymers.The microbial production of 1,3-propanediol presents several opportunities,and the final purity grade determines its price and commercial viability.The development of novel separation technology could improve the economic viability of the bioproduction of 1,3-propanediol.Thus,we investigated salting-out extraction as a novel process for 1,3-propanediol recovery from fermentation broth.Initially,a screening for the best salt/solvent combination was conducted and then optimized using the response surface methodology.The solvents studied were methanol,ethanol,isopropanol and acetone,and the salts examined were K_2HPO_4,Na_2CO_3,K_2CO_3,(NH_4)_2SO_4,NaHPO_4,K_3PO_4 and C_6H_5NaO_7.The optimal extraction system consisted of 34 wt%K_3PO_4,28 wt% ethanol,and 38 wt% fermentation broth containing 23.0 g·L^(-1)1,3-propanediol,which gave the highest partition coefficient of 33 and recovery yield of 97%.The results demonstrated that salting-out extraction was a promising method for 1,3-propanediol recovery from fermentation broth. | Daiana Wischral Hongxin Fu Fernando L.Pellegrini Pessoa Nei Pereira Jr Shang-Tian Yang | 2018 | Chinese Journal of Chemical Engineering2018，26，1： | 2 |
| 9 | Anthelmintic activity of essential oil of Ocimum gratissimum Linn. and eugenol against Haemonchus contortus显示文摘 | L.M Pessoa S.M Morais C.M.L Bevilaqua J.H.S Luciano | 2002 | Veterinary Parasitology2002，，1： | 2 |
| 10 | Family history and disease outcomes in patients with Crohn's disease:A comparison between China and the United States显示文摘AIM To investigate the differences in family history of inflammatory bowel disease(IBD) and clinical outcomes among individuals with Crohn's disease(CD) residing in China and the United States.METHODS We performed a survey-based cross-sectional study of participants with CD recruited from China and the United States.We compared the prevalence of IBD family history and history of ileal involvement,CD-related surgeries and IBD medications in China and the United States,adjusting for potential confounders.RESULTS We recruited 49 participants from China and 145 from the United States.The prevalence of family history of IBD was significantly lower in China compared with the United States(China:4.1%,United States:39.3%).The three most commonly affected types of relatives were cousin,sibling,and parent in the United States compared with child and sibling in China.Ileal involvement(China:63.3%,United States:63.5%) and surgery for CD(China:51.0%,United States:49.7%) were nearly equivalent in the two countries.CONCLUSION The lower prevalence of familial clustering of IBD in China may suggest that the etiology of CD is less attributed to genetic background or a family-shared environment compared with the United States.Despite the potential difference in etiology,surgery and ileal involvement were similar in the two countries.Examining the changes in family history during the continuing rise in IBD may provide further insight into the etiology of CD. | Pei-Qi Wang Jun Hu Elie S Al Kazzi Eboselume Akhuemonkhan Min Zhi Xiang Gao Raquel Holand de Paula Pessoa Sami Ghazaleh Tuhina Cornelius Suhel Abbas Sabunwala Shadi Ghadermarzi Kartikeya Tripathi Mark Lazarev Pin-Jin Hu Susan Hutfless | 2016 | World Journal of Gastrointestinal Pharmacology and Therapeutics2016，7，4： | 2 |
| 11 | Performance of LCD iterative method in the finite element and finite difference solution of convection-diffusion equations显示文摘 | Catabriga L Vail A Melotti Z Pessoa M Coutinho A | | Communications in numerical methods in engineering0，22，6： | 1 |
| 12 | How do emotion and motivation direct executive control?显示文摘 | Pessoa L | 2009 | Trends in Cognitive Sciences2009，13，4： | 1 |
| 13 | Midterm out- come of ultrasound-guided alcohol gel sclerotherapyfor symptomatic leiomyoma 显示文摘 | Pessoa LA Jr Gurgel F Avila M | 2010 | J Ultrasound Med2010，29，9： | 1 |
| 14 | Assessment of takotsubo ( ampulla ) eardiomyopathy using iodine-123 metaiodobenzylguanidine seintigraphy 显示文摘 | Pessoa PM Xavier SS Lima SL | 2006 | Acta Radiol2006，47，10： | 1 |
| 15 | Use of laccase together with redox mediators to decolourize Remazol Brilliant Blue R 显示文摘 | Graca M B Soares M T Pessoa de Amorim | 2001 | Journal of Bioteehnology2001，89，： | 1 |
| 16 | Neural processing of emotional faces requires attention显示文摘 | Pessoa L McKenna M Gutierrez E | | 0，，： | 1 |
| 17 | Use of laccase to- gether with redox mediators to decolourize Remazol Bril- liant Blue R显示文摘 | Graea MB Soares MT Pessoa A | 2001 | Journal of Bioteehnology2001，，89： | 1 |
| 18 | Characterisation of ultrafiltration and nanofillxation membranes from rejections of neutral reference solutes using a model of asymmetric pores 显示文摘 | Morro A Pessoa De Amorim M T Lopes A | 2008 | Journal of Membrane Science2008，319，1： | 1 |
| 19 | Propolis effects on pro-inflammatory cytokine production and Toll-like receptor 2 and 4 expression in stressed mice显示文摘 | Ana Carolina Pagliarone Cláudio Lera Orsatti Michelle Cristiane Búfalo Fabiane Missima Tatiana Fernanda Bachiega Jo?o Pessoa Araújo Júnior José Maurício Sforcin | 2009 | International Immunopharmacology2009，，11： | 1 |
| 20 | Efficacy and safety of endoscopic submucosal dissection for colorectal neoplasia: a systematic review显示文摘 | A. Repici C. Hassan D. De Paula Pessoa N. Pagano A. Arezzo A. Zullo R. Lorenzetti R. Marmo | 2012 | Endoscopy2012，，02： | 1 |
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