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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Multiple genetic alterations and behavior of cellular biology in gastric cancer and other gastric mucosal lesions:H.pylori infection,histological types and staging显示文摘AIM To investigate the expression of multiplegenes and the behavior of cellular biology ingastric cancer(GC)and other gastric mucosallesions and their relations to Helicobacter pylori(H.pylori)infection,tumor staging andhistological subtypes.METHODS Three hundred and twenty-sevenspecimens of gastric mucosa obtained viaendoscopy or surgical resection,and ABCimmunohistochemical staining were used todetect the expression of p53,p16,Bcl-2 andCOX-2 proteins.H.pylori was determined byrapid urea test combined with pathologicalstaining or14C urea breath test.Cellular image analysis was performed in 66 patients withintestinal metaplasia(IM)and/or dysplasia(Dys).In 30 of them,both cancer and theparacancerous tissues were obtained at the timeof surgery.Histological pattern,tumor staging,lymph node metastasis,grading ofdifferentiation and other clinical data werestudied in the medical records.RESULTS p16 expression of IM or Dys wassignificantly lower in positive H.pylori chronicatrophic gastritis(CAG)than those withnegative H.pylori(CAG:54.8% vs 88.0%,IM:34.4% vs 69.6%,Dys:23.8% vs 53.6%,allP<0.05),Bcl-2 or COX-2 expression of IM orDys in positive H.pylori cases was significantlyhigher than that without H.pylori(Bcl-2:68.8%vs23.9%,90.5% vs 60.7%;COX-2:50.0% vs10.8%,61.8% vs 17.8%;all P<0.05).Themean number of most parameters of cellularimage analysis in positive H.pylori group wassignificantly higher than that in negative H.pylori group(Ellipser:53±14,40±12μm,Area1:748±572,302±202 μm2,Area2:3050±1661,1681±1990 μm2,all P<0.05;Ellipseb:79±23,58±15 μm,Ratio1:22%±5%,13%±4%,Ratio2:79%±17%,53%±20%,all P<0.01).There was significant correlation between Bcl-2and histologic pattern of gastric carcinoma,andbetween COX-2 and tumor staging or lymph nodemetastasis(Bcl-2:75.0% vs 16.7%;COX-2:76.0% vs 20.0%,79.2% vs 16.7%;allP<0.05).CONCLUSION p1l6, Bcl-2, and COX-2 but not p53 gene may play a role in the early genesis/ progression of gastric carcinoma and are associated with H. pylori infection. p53 gene is relatively late event in gastric tumorigenesis and mainly relates to its progression. There is more cellular-biological behavior of malignant tumor in gastric mucosal lesions with H. pylori infection. Aberrant Bcl-2 protein expression appears to be preferentially associated with the intestinal type cancer. COX-2 seems to be related to tumor staging and lymph node metastasis. | Heng Jun Gao Lian Zhen Yu Jian Feng Bai Yan Shen Peng Gu Sun Han Lin Zhao Kun Miu Xiu Zhen Lü Xiao Yong Zhang Zhi Quan Zhao | 2000 | World Journal of Gastroenterology2000，6，6： | 52 |
| 3 | Quantification and analysis of anthocyanin and flavonoids compositions,and antioxidant activities in onions with three different colors显示文摘Onion(Allium cepa L.) is a very important vegetable crop widely cultivated throughout the world. In this study, we measured total anthocyanins and flavonols of onions of three colors. The fresh weight(FW) of total anthocyanins were(29.99±1.19),(9.64±1.30) and(0.75±0.40) mg 100 g^(–1) fruit in red, yellow and white onions, respectively. Likewise, the FW of total flavonoids were(111.10±5.98),(36.64±3.59), and 0 mg 100 g^(–1) in red, yellow and white, respectively. Four types of anthocyanins(delphinidin 3,5-diglycosides, cyanidin 3,5-diglycosides, cyanidin 3-glycosides and cyanidin 3-(6′′-malonyl)-glucopyranoside) and two kinds of flavonoids(quercetin and quercetin 3-glycosides) were identified in two varieties(red and yellow bulb) of onions by HPLC/DAD-ESI/MS. The total polyphenol contents were also measured by means of Folin-Ciocalteu method. Moreover, all the concentrations of anthocyanins, flavonoids and polyphenols showed significantly positive correlations with antioxidant activities measured by DPPH˙, ABTS˙+ and FRAP assays. This study provided information on anthocyanin and flavonoids compositions that will be useful for onion breeding. | ZHANG Shi-lin DENG Peng XU Yu-chao Lü Shan-wu WANG Jian-jun | 2016 | Journal of Integrative Agriculture2016，15，9： | 25 |
| 4 | 颈椎前路减压融合术对颈椎病眩晕患者的缓解效果——多中心前瞻性队列研究显示文摘颈椎病患者除颈部疼痛外常伴有眩晕症状,这种颈源性眩晕并非由前庭病理引起。研究表明,退行性椎间盘突出症与颈椎病患者的退行性眩晕有关,而颈椎前路椎间盘切除融合手术可缓解这一症状。本项多中心队列研究的目的在于分析颈椎前路减压(anterior cervical decompression,ACD)手术对颈源性眩晕的缓解效果,同时探讨其发病机制。 | Peng B Yang L Yang C 李洪吉 王建华 | 2018 | 中国骨科临床与基础研究杂志2018，10，4： | 15 |
| 5 | miR-182参与调节胶质母细胞瘤的凋亡、生长和分化(英文)显示文摘Glioblastoma multiforme(GBM)is a lethal,therapy-resistant brain cancer consisting of numerous tumor cell subpopulations,including stem-like glioma-initiating cells(GICs),which contribute to tumor recurrence following initial response to therapy.Here,we identified miR-182 as a regulator of apoptosis,growth,and differentiation programs whose expression level is correlated with GBM patient survival.Repression of Bcl2-like12(Bcl2L12),c-Met,and hypoxia-inducible factor 2α(HIF2A)is of central importance to miR-182 anti-tumor activity,as it results in enhanced therapy susceptibility,decreased GIC sphere size,expansion,and stemness in vitro.To evaluate the tumor-suppressive function of miR-182 in vivo,we synthesized miR-182-based spherical nucleic acids(182-SNAs);i.e.,gold nanoparticles covalently functionalized with mature miR-182 duplexes.Intravenously administered 182-SNAs penetrated the bloodbrain/blood-tumor barriers(BBB/BTB)in orthotopic GBM xenografts and selectively disseminated throughout extravascular glioma parenchyma,causing reduced tumor burden and increased animal survival.Our results indicate that harnessing the anti-tumor activities of miR-182 via safe and robust delivery of 182-SNAs represents a novel strategy for therapeutic intervention in GBM. | Kouri FM Hurley LA Daniel WL Day ES Hua Y Hao L Peng CY Merkel TJ Queisser MA Ritner C Zhang H James CD Sznajder JI Chin L Giljohann DA Kessler JA Peter ME Mirkin CA Stegh AH | 2015 | 中华神经外科疾病研究杂志2015，14，2： | 14 |
| 6 | Aberrant expression of ether à go-go potassium channel in colorectal cancer patients and cell lines显示文摘AIM: To study the expression of ether à go-go (Eag1) potassium channel in colorectal cancer and the relation- ship between their expression and clinico-pathological features. METHODS: The expression levels of Eag1 protein were determined in 76 cancer tissues with paired non- cancerous matched tissues as well as 9 colorectal adenoma tissues by immunohistochemistry. Eag1 mRNA expression was detected in 13 colorectal cancer tissues with paired non-cancerous matched tissues and 4 colorectal adenoma tissues as well as two colorectal cancer cell lines (LoVo and HT-29) by reverse transcription PCR. RESULTS: The frequency of positive expression of Eag1 protein was 76.3% (58/76) and Eag1 mRNA was 76.9% (10/13) in colorectal cancer tissue. Expression level of Eag1 protein was dependent on the tumor size, lymphatic node metastasis, other organ metastases and Dukes’ stage (P < 0.05), while not dependent on age, sex, site and degree of differentiation. Eag1 protein and mRNA were negative in normal colorectal tissue, and absolutely negative in colorectal adenomas except that one case was positively stained for Eag1 protein. CONCLUSION: Eag1 protein and mRNA are aberrantly expressed in colorectal cancer and occasionally expressed in colorectal adenoma. The high frequency of expression of Eag1 in tumors and the restriction of normal expression to the brain suggest the potential of this protein for diagnostic, prognostic and therapeutic purposes. | Xiang-Wu Ding Juan-Juan Yan Ping An Peng Lü He-Sheng Luo | 2007 | World Journal of Gastroenterology2007，13，8： | 10 |
| 7 | Approximate Solution of Oil Film Load-carrying Capacity of Turbulent Journal Bearing with Couple Stress Flow显示文摘The instability of the rotor dynamic system supported by oil journal bearing is encountered frequently, such as the half-speed whirl of the rotor, which is caused by oil film lubricant with nonlinearity. Currently, more attention is paid to the physical characteristics of oil film due to an oil-lubricated journal bearing being the important supporting component of the bearing-rotor systems and its nonlinear nature. In order to analyze the lubrication characteristics of journal bearings efficiently and save computational efforts, an approximate solution of nonlinear oil film forces of a finite length turbulent journal bearing with couple stress flow is proposed based on Sommerfeld and Ocvirk numbers. Reynolds equation in lubrication of a finite length turbulent journal bearing is solved based on multi-parametric principle. Load-carrying capacity of nonlinear oil film is obtained, and the results obtained by different methods are compared. The validation of the proposed method is verified, meanwhile, the relationships of load-carrying capacity versus eccentricity ratio and width-to-diameter ratio under turbulent and couple stress working conditions are analyzed. The numerical results show that both couple stress flow and eccentricity ratio have obvious influence on oil film pressure distribution, and the proposed method approximates the load-carrying capacity of turbulent journal bearings efficiently with various width-to-diameter ratios. This research proposes an approximate solution of oil film load-carrying capacity of turbulent journal bearings with different width-to-diameter ratios, which are suitable for high eccentricity ratios and heavy loads. | ZHANG Yongfang WU Peng GUO Bo L Yanjun LIU Fuxi YU Yingtian | 2015 | Chinese Journal of Mechanical Engineering2015，28，1： | 10 |
| 8 | 兼具双面黏性和生物活性的多功能水凝胶显示文摘用于创伤愈合以及软组织修复创新点水凝胶是一种具有三维交联网络的生物医用材料,既可以作为机体保护屏障防止细菌感染,又能为组织再生提供合适的微环境。对机体组织和植入物具有双面黏性的水凝胶具有巨大的临床应用潜力。一方面,对组织具有高黏合性的水凝胶可以把受损组织黏合在一起,防止因常规缝合引起的瘢痕组织形成。 | GAO L ZHOU Y L PENG J L | 2019 | 张江科技评论2019，0，6： | 9 |
| 9 | Land cover mapping using time series HJ-1 / CCD data显示文摘It is very difficult to have remote sensing data with both high spatial resolution and high temporal frequency; thus, two categories of land-use mapping methodology have been developed separately for coarser resolution and finer resolution data. The first category uses time series of data to retrieve the variation of land surface for classification, which are usually used for coarser resolution data with high temporal frequency. The second category uses fine spatial resolution data to classify different land surface. With the launch of Chinese satellite constellation HJ-1in 2008, four 30 m spatial resolution CCDs with about 360 km coverage for each one onboard two satellites made a revisit period of two days, which brought a new type of data with both high spatial resolution and high temporal frequency. Therefore, by taking the spatiotemporal advantage of HJ-1/CCD data we propose a new method for finer resolution land cover mapping using the time series HJ-1/CCD data, which can greatly improve the land cover mapping accuracy. In our two study areas, the very high resolution remote sensing data within Google Earth are used to validate the land cover mapping results, which shows a very high mapping accuracy of 95.76% and 83.78% and a high Kappa coefficient of 0.9423 and 0.8165 in the Dahuofang area of Liaoning Province and the Heiquan area of Gansu Province respectively. | ZHONG Bo MA Peng NIE AiHua YANG AiXia YAO YanJuan Lü WenBo ZHANG Hang LIU QinHuo | 2014 | Science China Earth Sciences2014，57，8： | 9 |
| 10 | Superior grains determined by grain weight are not fully correlated with the flowering order in rice显示文摘Rice panicles are composed of many branches with two types of extreme grains,the superior and the inferior.Traditionally,it has been well accepted that earlier flowers result in superior grains and late flowers generate inferior grains.However,these correlations have never been strictly examined in practice.In order to determine the accurate relationship between superior and inferior grains and the flowering order,we localized all the seeds in a panicle in four distinct rice species and systematically documented the rice flowering order,flower locations and the final grain weight for their relationships.Our results demonstrated that the grain weight is more heavily determined by the position of the seeds than by the flowering order.Despite earlier flowering has a positive correlation with the grain weight in general,grains from flowers blooming on the second day after anthesis generally gained the highest weight.This suggests earlier flowers may not result in superior grains.Therefore,we concluded that superior and inferior grains,commonly determined by grain weight,are not fully correlated with the flowering order in rice.Following the order of the grain weight,the superior grains are generally localized at the middle parts of the primary branches,whereas inferior grains were mainly on the last two secondary branches of the lower half part of the panicle.In addition,the weight of inferior grains were affected by spikelet thinning and spraying with exogenous plant growth regulators,indicating that physiological incompetence might be the major reason for the occurrence of the inferior grains. | PENG Ting L Qiang ZHAO Ya-fan SUN Hong-zheng HAN Ying-chun DU Yan-xiu ZHANG Jing LI Jun-zhou WANG Lin-lin ZHAO Quan-zhi | 2015 | Journal of Integrative Agriculture2015，14，5： | 7 |
| 11 | Recent progresses in application of functionalized graphene sheets显示文摘Graphene,a rapidly rising star on the horizon of material science,has a unique two-dimensional nanostructure as well as exceptional mechanical and electronic properties.Despite its short history,graphene has exhibited great potential in various applications.In order to implement the potential applications,functionalization of graphene is necessary to obtain uniform dispersions for good processability.Two kinds are dominant for functionalization such as covalent and non-covalent methods.The former is based on the formation of covalent bonds,and the latter the interaction among molecules.In this review,we summarized briefly the recent progress of functionalized graphene sheets (FGs) in different fields,such as optoelectronic materials,sensors,energy storage materials,catalytic,reinforcing components and so on,and also prospected the development trend of FGs in the future. | Lü Peng,FENG YiYu,ZHANG XueQuan,LI Yu & FENG Wei School of Materials Science and Engineering,Tianjin University,Tianjin 300072,China | 2010 | Science China(Technological Sciences)2010，53，9： | 7 |
| 12 | Hydrodynamic process of Tibetan Plateau lake revealed by grain size:Case study of Pumayum Co显示文摘Under the background of global warming,some lakes on the Tibetan Plateau(TP) are potentially sensitive to temperature change.With a case study of Pumayum Co,where glacier meltwater is important to supply(we call this a glacier-fed lake hereafter),we analyze the sensitivity of lake sediment grain size to temperature change.This is done by resolving the modern hydrodynamic process,coupled with comparison of paleoclimatic proxies.According to the spatial distributions of parameters,percentage of grain size and the grain size frequency distribution curve,hydrodynamic processes are analyzed.Five clastic sedimentation types are thereby discriminated.In the open lake area,suspended load transport is the main transport agent.Grain Size Trend Analysis(GSTA),a sediment dynamics model,reveals a trend toward eastward transport.This indicates that the largest and glacier-fed river,the Jiaqu River,influences the entire lake(not just the subsurface alluvial fan),and that lake sediment grain size may serve as a temperature indicator.Time series comparison between grain size of a short core from the central lake and meteorological data confirms this temperature indication,which in turn shows reliability of the method discriminating the hydrodynamic process.This case study will improve the ability of paleoclimatic reconstruction using lake sediment in glacier-fed lakes on the Tibetan Plateau. | JU JianTing ZHU LiPing FENG JinLiang WANG JunBo WANG Yong XIE ManPing PENG Ping ZHEN XiaoLin LÜXinMiao | 2012 | Chinese Science Bulletin2012，57，19： | 7 |
| 13 | Early experiences on living donor liver transplantation in China: multicenter report显示文摘Background Because of the lack of brain death laws in China, the proportion of cadaveric organ donation is low. Many patients with end-stage liver disease die waiting for a suitable donor. Living donor liver transplantation (LDLT) would reduce the current discrepancy between the number of patients on the transplant waiting list and the number of available organ donors. We describe the early experience of LDLT in the mainland of China based on data from five liver transplant centers. Methods Between January 2001 and October 2003, 45 patients with end-stage liver disease received LDLT at five centers in China. The indication and timing, surgical techniques and complications, nonsurgical issues including rejection, infection, and advantages of LDLT in the series were reviewed. Actuarial patient and graft survival rates were calculated by using the Kaplan-Meier product-limit estimate. Statistical analysis was completed by using SPSS 10.0. Results All LDLT recipients were cirrhotic patients, except for one man with fulminant hepatic failure. Among the 45 cases of LDLT, 35 (77.8%) were performed in one center (the First Affiliated Hospital of Nanjing Medical University). The overall 1 and 3 year survival rate of the recipients was 93.1% and 92.0%, respectively. Of the 45 LDLT donors, there were 3 cases of biliary leakage, 2 subphrenic collections, 1 fat liquefaction around the incision and 1 biliary peritonitis after T tube removal. All donors recovered completely. Conclusions LDLT provides an excellent approach to addressing the problem of donor shortage in China even though the operation is complicated, uncompromising and difficult with respect to the safety of the donors and receptors. Despite early technical hurdles having been overcome, perfection of technique is still necessarily. At present, LDLT is a good choice for the patients with irreversible liver disease. | WANG Xue-hao YAN Lü-nan ZHANG Feng LI Xiang-cheng ZHU Ji-ye PENG Zhi-hai LIU Jin-hui LI Guo-qiang CHENG Feng SUN Bei-cheng GE Wen-gang | 2006 | Chinese Medical Journal2006，，12： | 7 |
| 14 | Soil pH Dynamics and Nitrogen Transformations Under Long-Term Chemical Fertilization in Four Typical Chinese Croplands显示文摘Long-term fertilization experiment provides the platform for understanding the proton budgets in nitrogen transformations of agricultural ecosystems.We analyzed the historical(1990-2005) observations on four agricultural long-term experiments in China(Changping,Chongqing,Gongzhuling and Qiyang) under four different fertilizations,i.e.,no-fertilizer(control),sole chemical nitrogen fertilizer(F N),sole chemical phosphorous and potassium fertilizers(F PK) and chemical nitrogen,phosphorous and potassium fertilizers(F NPK).The significant decline in topsoil pH was caused not only by chemical N fertilization(0.29 and 0.89 pH at Gongzhuling and Qiyang,respectively) but also by chemical PK fertilization(0.59 pH at Gongzhuling).The enhancement of available nutrients in the topsoil due to long-term direct nutrients supply with chemical fertilizers was in the descending order of available P(168-599%)>available K(16-189%)>available N(9-33%).The relative rate of soil pH decline was lower under long-term judicious chemical fertilization(-0.036-0.034 pH yr-1) than that under long-term sole N or PK fertilization(0.016-0.086 pH yr-1).Long-term judicious chemical fertilization with N,P and K elements decreases the nutritional limitation to normal crop growth,under which more N output was distributed in biomass removal rather than the loss via nitrate leaching.We concluded that the N distribution percentage of nitrate leaching to biomass removal might be a suitable indicator to the sensitivity of agricultural ecosystems to acid inputs. | MENG Hong-qi XU Ming-gang L Jia-long HE Xin-hua LI Jian-wei SHI Xiao-jun PENG Chang WANG Bo-ren ZHANG Hui-min | 2013 | Journal of Integrative Agriculture2013，12，11： | 6 |
| 15 | Utilization of gold-bearing and iron-rich pyrite cinder via a chlorination–volatilization process显示文摘The chlorination-volatilization process has been adopted to make full use of gold-bearing and iron-rich pyrite cinder. However, problems of low recovery rate, pulverization of pellets, and ring formation have been encountered during the industrialization of this process. The effects of various parameters on the volatilization rates of valuable metals and on the compressive strength of roasted pellets were investigated in this paper. The parameters include the CaCl_2 dosage, heating temperature, and holding time. The results show that heating temperature is the most important parameter for the recovery of target metals. More CaCl_2 was needed for the recovery of zinc than for the recovery of gold, silver, and lead. CaCl_2 started to react with sulfides/SO_2/SiO_2 at temperatures below the melting point of CaCl_2 to generate Cl_2/HCl. Gaseous CaCl_2 was formed at higher temperatures and could react with any of the components. The compressive strength of roasted CaCl_2-bearing pellets first decreased slowly with increasing temperature at temperatures lower than 873 K, which could result in the pulverization of pellets during heating. Their compressive strength increased dramatically with increasing temperature at temperatures greater than 1273 K. Certain quantities of CaCl_2 and Fe(Ⅱ) could improve the compressive strength of the roasted pellets; however, the addition of excessive CaCl_2 decreased the compressive strength of pellets. | Jian Ding Pei-wei Han Cui-cui Lü Peng Qian Shu-feng Ye Yun-fa Chen | 2017 | International Journal of Minerals,Metallurgy and Materials2017，24，11： | 6 |
| 16 | Interleukin-1α, 6 regulate the secretion of vascular endothelial growth factor A, C in pancreatic cancer显示文摘Vascular endothelial growth factor (VEGF, namely VEGF-A) is an angiogenic polypeptide and VEGF-C is a lymphangiogenic polypeptide that has been implicated in cancer growth, invasion and metastasis. Several cytokines and growth factors play an important part in cancer progression. These cytokines and growth factors are the principal mediators of cancer cells-stromal cell interaction , which is critical for invasion of cancer cells to the surrounding tissues and metastatic dissemination to distant organs. In this study, we studied VEGF-A, C expression in cultured human pancreatic cancer cell lines and whether the presence of VEGF-A, C in the cell lines is regulated by cytokines interleukin-lct (EL-1α), and interleukin-6 (IL-6). METHODS: We used Northern blot and Western blot methods to analyze expression of the gene and protein of VEGF-A, C in all 6 tested cell lines (ASPC-1, CAPAN-1, MIA-PaCa-2, PANC-1, COLO-357 and T3M4) respectively. To analyze what is the regulator for this VEGF-A, C expression in pancreatic cancer,we used the reverse transcription -polymerase chain reaction (RT-PCR) method to analyze VEGF-A, C expression in cultured human pancreatic cancer cell lines (CAPAN-1 and COLO-357) under the stimulation with IL-1α (10μg/L) or IL-6 (100 μg/L). RESULTS:Northern blot analysis revealed the presence of the 4.1-kb VEGF-A mRNA transcript and 2.4-kb VEGF-C mRNA transcript in all 6 tested cell lines. Immunoblotting with highly specific anti-VEGF-A, anti-VEGF-C antibody revealed the presence of a molecular weight of 43-kDa VEGF-A protein and 55-kDa VEGF-C protein in all the cell lines. RT-PCR analysis revealed the levels of the VEGF-A and VEGF-C gene were 1-2 fold and a 1-fold increase in the COLO-357 cell line by stimulation with IL-la, however, no effect was found in the CAPAN-1 cell line. The levels of the VEGF-A and VEGF-C gene were 2-5 fold and a 1-fold increase in the CAPAN-1 cell line by stimulation with IL-6, but, no effect was found in the COLO-357 cell line. CONCLUSION:These findings suggested that the expression of VEGF-A, C and their regulation by IL-1α, IL-6 in pancreatic cancer contributes to the lymphatic and distant metastasis and the disease progression. | Department of Hepatobiliary Surgery (Tang RF, Zhang FR, Peng L, Wang SX, Xiao Y and Zhang M) and Department of Dermatology (Wang SX), 4th Hospital, Hebei Medical University, Shijiazhuang 050011, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 6 |
| 17 | SPSBl-mediated HnRNP A1 alternative splicing and cell ubiquitylation regulates migration in EGF signaling显示文摘细胞外的信号被显示了影响拼接的其他的 pre-mRNA;然而,分子的机制和导致信号的拼接的规定的生物意义仍然保持大部分未知。这里,我们报导那个表皮的生长因素(EGF ) 通过一个著名拼接管理者的 ubiquitylation 导致拼接的变化, hnRNP A1。EGF 发信号 upregulates 一个 E3 ubiquitin (Ub ) ligase 适配器,敏捷的包含域的 SOCS 盒子蛋白质 1 (SPSB1 ) ,它结合离氨酸 29 连接 polyUb 链到 hnRNP A1 上的成员 Elongin B/C-Cullin 建筑群。重要地, hnRNP A1 的 SPSB1 和 ubiquitylation 在驾驶 EGF 的房间移植有一个关键角色。机械学地,和 SR 蛋白质 kinases (SRPK ) 的激活的 hnRNP A1 的导致 EGF 的 ubiquitylation 导致拼接的 Rac1 的 upregulation isoform, Rac1b,支持房间活动性。这些调查结果解开在蛋白质 ubiquitylation 并且在 EGF/EGF 受体发信号的其他的拼接之间的新奇串音,并且在 modulating 房间移植识别新 EGF/SPSB1/hnRNP A1/Rac1 轴,它可以为癌症治疗有重要含意。 | Feng Wang Xing Fu Peng Chen Ping Wu Xiaojuan Fan Na Li Hong Zhu Ting-Ting Jia Hongbin Ji Zefeng Wang Catherine C L Wong Ronggui Hu Jingyi Hui | 2017 | Cell Research2017，27，4： | 5 |
| 18 | Surgical treatment for primary angle closure-glaucoma: a Meta analysis显示文摘AIM:To evaluate the efficacy and safety of trabeculectomy,phacotrabeculectomy plus intraocular lens implantation(phacotrab+IOL group) and phacoemulsification with IOL(phaco+IOL) in primary angle-closure glaucoma(PACG).METHODS: It was a systematic review and meta-analysis,randomized controlled trials(RCT) and clinical controlled trials(CCT) were collected through electronic searches of the Cochrane Library,PubMed,EMbase,Wanfang Database online,Chinese journal Full-text Database,Chinese Scientific Journals Full-text Database (from the date of building the database to October 2010) We also checked the bibliographies of retrieved articles.All the related data that matched our standards were abstracted.The quality of included trials was evaluated according to the Dutch Cochrane Centre.RevMan 5.0 software was used for Meta-analysis.RESULTS: A total of 5 RCT and 11 CCT involving 1495 eyes were included.The results of meta-analysis showed that phacotrab+IOL group was superior than trabeculectomy(trab group) (MD -3.93,95%CI [-7.31,-0.54]) which was also superior than phaco+IOL group(MD 0.52,95%CI [0.10,0.95]) in decreasing Intraocular Pressure(IOP).Phacotrab group(MD -1.45,95%CI [-1.68,-1.22])and phaco group (MD-1.12,95%CI [-1.87,-0.37])are both deeper than trab group in the anterior chamber depth.In increasing the coefficient of outflow facility of aqueous humor(C values) there was no statistical difference in the three groups.And there was no statistical difference between phacotrab groups and phaco groups in visual acuity but phacotrab group was superior than phaco group (MD 1.07,95%CI [0.73,1.40])in the use of IOP-lowering drugs.There was no statistical difference among three groups.CONCLUSION: Current evidence suggests that phacotrab+ IOL group was superior than trab group which was also superior than phaco+IOL group in decreasing IOP.Phacotrab group and phaco group are both deeper than trab group in the anterior chamber depth.Phacotrab group was superior than phaco group in the use of IOP-lowering drugs. | Bo-Lin Deng, Jing Tian | 2011 | International Journal of Ophthalmology(English edition)2011，4，3： | 5 |
| 19 | Improvement of mechanical properties of Sn-58Bi alloy with multi-walled carbon nanotubes显示文摘Carbon nanotubes (CNTs) reinforced Sn-58Bi composites were successfully fabricated through ball-milling method and low temperature melting process.The influence of multi-walled carbon nanotubes (MWCNTs) on the mechanical strength and ductility of Sn-58Bi lead-free alloy was studied.The mechanical test results show that the bending strength of Sn-58Bi-0.03CNTs (mass fraction,%) composite is increased by 10.5% than that of the Sn-58Bi alloy,which can be attributed to the reduction of Sn-rich segregation and the grain refinement.The toughness of Sn-58Bi-0.03CNTs composite is increased by 48.9% than that of the matrix materials.It is indicated that the influence of CNTs on the strength of Sn-58Bi-xCNTs composite is insignificant.In addition,the fracture mechanism of CNTs reinforced Sn Bi composite was analyzed.The corresponding fracture surface comparison between the Sn-58Bi-0.03CNTs composite and the monolithic Sn-58Bi alloy was made to identify the influence of CNTs on the fracture behavior and the reinforcing effect of CNTs. | HE Peng Lü Xiao-chun LIN Tie-song LI Hai-xin AN Jing MA Xin FENG Ji-cai ZHANG Yan LI Qi QIAN Yi-yu | 2012 | 中国有色金属学会会刊:英文版2012，22，S3： | 5 |
| 20 | Separation of chalcopyrite and pyrite from a copper tailing by ammonium humate显示文摘Copper tailings constitute a large proportion of mine wastes. Some of the copper tailings can be recycled to recover valuable minerals. In this paper, a copper tailing was studied through the chemical analysis method, Xray diffraction and scanning electron microscope-energy dispersive spectrum. It turned out that chalcopyrite(Cu) and pyrite(S) were the main recoverable minerals in the tailing. In order to separate chalcopyrite from pyrite in low pulp pH, ammonium humate(AH) was singled out as the effective regulator. The depression mechanism of AH on the flotation of pyrite was proved by FTIR spectrum and XPS spectrum, demonstrating that there was a chemical adsorption between AH and pyrite. By Response Surface Methodology(RSM), the interaction between AH, pulp pH and iso-butyl ethionine(Z200) was discussed. It was illustrated that the optimal dosage of AH was 1678 g·t^(-1) involving both the recovery of Cu and S. The point prediction by RSM and the closed-circuit flotation displayed that the qualified Cu concentrate and S concentrate could be obtained from the copper tailing.The study indicated that AH was a promising pyrite depressor in the low pulp pH from copper tailings. | Cuicui Lü Yongliang Wang Peng Qian Ya Liu Guoyan Fu Jian Ding Shufeng Ye Yuanfa Chen | 2018 | Chinese Journal of Chemical Engineering2018，26，9： | 5 |
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