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| 1 | Overexpression of the M2 isoform of pyruvate kinase is an adverse prognostic factor for signet ring cell gastric cancer显示文摘AIM:To investigate M2 isoform of pyruvate kinase(PKM2) expression in gastric cancers and evaluate its potential as a prognostic biomarker and an anticancer target.METHODS:All tissue samples were derived from gastric cancer patients underwent curative gastrectomy as a primary treatment.Clinical and pathological information were obtained from the medical records.Gene expression microarray data from 60 cancer and 19 noncancer gastric tissues were analyzed to evaluate the expression level of PKM2 mRNA.Tissue microarrays were constructed from 368 gastric cancer patients.Immunohistochemistry was used to measure PKM2 expression and PKM2 positivity of cancer was determined by proportion of PKM2-positive tumor cells and staining intensity.Association between PKM2 expression and the clinicopathological factors was evaluated and the correlation between PKM2 and cancer prognosis was evaluated.RESULTS:PKM2 mRNA levels were increased more than 2-fold in primary gastric cancers compared to adjacent normal tissues from the same patients(log transformed expression level:7.6 ± 0.65 vs 6.3 ± 0.51,P < 0.001).Moreover,differentiated type cancers had significantly higher PKM2 mRNA compared to undifferentiated type cancers(log transformed expression level:7.8 ± 0.70 vs 6.7 ± 0.71,P < 0.001).PKM2 protein was mainly localized in the cytoplasm of primary cancer cells and detected in 144 of 368(39.1%) human gastric cancer cases.PKM2 expression was not related with stage(P = 0.811),but strongly correlated with gastric cancer differentiation(P < 0.001).Differentiated type cancers expressed more PKM2 protein than did the undifferentiated ones.Well differentiated adenocarcinoma showed 63.6% PKM2-positive cells;in contrast,signet-ring cell cancers showed only 17.7% PKM2-positive cells.Importantly,PKM2 expression was correlated with shorter overall survival(P < 0.05) independent of stage only in signet-ring cell cancers.CONCLUSION:PKM2 expression might be an adverse prognostic factor for signet-ring cell carcinomas.Its function and potential as a prognostic marker should be further verified in gastric cancer. | Jae Yun Lim Sun Och Yoon So Young Seol Soon Won Hong Jong Won Kim Seung Ho Choi Jae Yong Cho | 2012 | World Journal of Gastroenterology2012，18，30： | 19 |
| 2 | Overexpression of miR-196b and HOXA10 characterize a poor-prognosis gastric cancer subtype显示文摘AIM:To identify molecular biologic differences between two gastric adenocarcinoma subgroups presenting different prognoses through the analysis of microRNA and protein expression.METHODS:Array technologies were used to generate1146 microRNAs and 124 proteins expression profiles of samples from 60 patients with gastric cancer.For the integrative analysis,we used established mRNA expression data published in our previous study.Whole mRNA expression levels were acquired from microarray data for 60 identical gastric cancer patients.Two gastric adenocarcinoma subgroups with distinct mRNA expression profiles presented distinctly different prognoses.MicroRNA and protein expression patterns were compared between gastric cancer tissue and normal gastric tissue and between two different prognostic groups.Aberrantly expressed microRNA,associated mRNA,and protein in patients with poor-prognosis gastric cancer were validated by quantitative reverse transcription polymerase chain reaction and immunochemistry in independent patients.RESULTS:We obtained the expression data of 1146microRNAs and 124 cancer-related proteins.Four microRNAs were aberrantly expressed in the two prognostic groups and in cancer vs non-cancer tissues(P<0.05).In the poor-prognosis group,miR-196b,miR-135b,and miR-93 were up-regulated and miR-29c*was down-regulated.miR-196b expression positively correlated with Homeobox A10(HOXA10)expression(r=0.726,P<0.001),which was significantly increased in poor-prognosis patients(P<0.001).Comparing gastric cancer with non-cancer tissues,46/124 proteins showed differential expression(P<0.05);COX2(P<0.001)and cyclin B1(P=0.017)were clearly overexpressed in the poor-prognosis group.CONCLUSION:Co-activation of miR-196b and HOXA10characterized a poor-prognosis subgroup of patients with gastric cancer.Elucidation of the biologic function of miR-196b and HOXA10 is warranted. | Jae Yun Lim Sun Och Yoon So-Young Seol Soon Won Hong Jong Won Kim Seung Ho Choi Ju-Seog Lee Jae Yong Cho | 2013 | World Journal of Gastroenterology2013，19，41： | 10 |
| 3 | NAPPA-QOL中文版的跨文化调适显示文摘目的对英文版的NAPPA-QOL进行跨文化调适,建立NAPPA-QOL量表中文版,并对其信度和效度进行初步评价。方法对NAPPA-QOL英文版进行正向翻译、综合协调、反向翻译、专家委员会评议,形成中文NAPPA-QOL预试验版。使用预试验版对30例指(趾)甲银屑病患者进行预调查,进一步修订量表,建立正式中文版NAPPA-QOL。通过访谈患者评价NAPPA-QOL量表表面效度和内容效度,采用Cronbach’sα系数和分半信度评价内部一致性,Spearman相关分析检验聚合效度。结果 30例患者中有27例(90.0%)对所有条目完全理解;NAPPA-QOL中文版总分和3个维度的Cronbach’sα系数在0.77~0.91之间,分半信度为0.80。除条目1和13外,其余条目与所在维度的相关系数均〉0.5,而与其他维度的相关系数则较弱。结论跨文化调适后的NAPPA-QOL中文版具有较好的信度和效度,适用于中国文化背景下指(趾)甲银屑病患者的生存质量评估。下一步可对中文版NAPPA-QOL量表的测量性能进行大样本数据采集和评估。 | 夏萍 卢传坚 吴大嵘 李艳 倪小佳 Shelley Ochs Hunter Hu 杨波 欧爱华 闫玉红 | 2016 | 现代预防医学2016，43，16： | 6 |
| 4 | The new proximal femoral nail antirotation (PFNA ? ) in daily practice: Results of a multicentre clinical study显示文摘 | R.K.J. Simmermacher J. Ljungqvist H. Bail T. Hockertz A.J.H. Vochteloo U. Ochs Chr.v.d. Werken | 2008 | Injury2008，，8： | 5 |
| 5 | Role of estrogen in angiogenesis in cardiovascular diseases显示文摘The formation of new blood vessels from existing ones is a major process of angiogenesis and it is most effective in the vascular systems。 The physiological process like hypoxia inducible factors involved in the regeneration of damaged tissues varies within the vascular systems in the endothelium and could be limited due to some major angiogenic growth factors like vascular endothelial growth factor, fibroblast growth factors and epidermal growth factor among others which bring about this cellular vascular regrowth。 These physiological processes leading to cellular vascular regrowth could be a major function for the treatment of cardiovascular diseases such as ischemia and atherosclerosis。 Estrogens are one of the known factors within the cellular mechanisms that could initiate repairs to the damaged vascular tissues, since estrogens are known inducers of angiogenesis leading to this cellular regrowth。 Research has also shown that this cellular regrowth is induced by vascular angiogenic growth factors via the estrogen receptors。 In this review we will attempt to summarize the main angiogenic growth factors involved in these physiological processes leading to angiogenesis and possible new mechanisms that could lead to this vascular regrowth。 And also we will try to summarize some reports on the effect of estrogen on these physiological processes leading to angiogenesis in cardiovascular diseases。 | Oche Barnabas Hong Wang Xiu-Mei Gao | 2013 | Journal of Geriatric Cardiology2013，10，4： | 5 |
| 6 | Lrp1 in osteoblasts controls osteoclast activity and protects against osteoporosis by limiting PDGF–RANKL signaling显示文摘Skeletal health relies on architectural integrity and sufficient bone mass, which are maintained through a tightly regulated equilibrium of bone resorption by osteoclasts and bone formation by osteoblasts. Genetic studies have linked the gene coding for low-density lipoprotein receptor-related protein1(Lrp1) to bone traits but whether these associations are based on a causal molecular relationship is unknown. Here, we show that Lrp1 in osteoblasts is a novel regulator of osteoclast activity and bone mass.Mice lacking Lrp1 specifically in the osteoblast lineage displayed normal osteoblast function but severe osteoporosis due to highly increased osteoclast numbers and bone resorption. Osteoblast Lrp1 limited receptor activator of NF-κB ligand(RANKL) expression in vivo and in vitro through attenuation of platelet-derived growth factor(PDGF-BB) signaling. In co-culture, Lrp1-deficient osteoblasts stimulated osteoclastogenesis in a PDGFRβ-dependent manner and in vivo treatment with the PDGFR tyrosine kinase inhibitor imatinib mesylate limited RANKL production and led to complete remission of the osteoporotic phenotype. These results identify osteoblast Lrp1 as a key regulator of osteoblast-to-osteoclast communication and bone mass through a PDGF–RANKL signaling axis in osteoblasts and open perspectives to further explore the potential of PDGF signaling inhibitors in counteracting bone loss as well as to evaluate the importance of functional LRP1 gene variants in the control of bone mass in humans. | Alexander Bartelt Friederike Behler-Janbeck F.Timo Beil Till Koehne Brigitte Müller Tobias Schmidt Markus Heine Laura Ochs Tayfun Yilmaz Martin Dietrich Jan P.Tuckermann Michael Amling Joachim Herz Thorsten Schinke Joerg Heeren Andreas Niemeier | 2018 | Bone Research2018，6，1： | 5 |
| 7 | The management of ascites in cirrhosis: Report on the consensus conference of the International Ascites Club显示文摘 | Kevin P. Moore Florence Wong Pere Gines Mauro Bernardi Andreas Ochs Francesco Salerno Paolo Angeli Michael Porayko Richard Moreau Guadelupe Garcia-Tsao Wladimiro Jimenez Ramon Planas Vicente Arroyo | 2003 | Hepatology2003，，1： | 4 |
| 8 | Thioredoxin and thioredoxin-interacting protein as prognostic markers for gastric cancer recurrence显示文摘AIM:To evaluate the potential of thioredoxin (TXN) and thioredoxin-interacting protein (TXNIP) expression as biomarkers for predicting gastric cancer recurrence. METHODS:TXN and TXNIP expression levels were acquired from gene expression microarray data for 65 human gastric cancer tissues. We determined whether each gene expression level was associated with cancer recurrence and investigated the relationship between the two genes. For validation, the expression levels of TXN and TXNIP were measured by quantitative real- time reverse transcription polymerase chain reaction in 68 independent stage Ⅲ gastric cancer patients. The correlation between gene expression and cancer prognosis was evaluated. Immunohistochemical staining was performed to investigate the protein expression levels of TXN and TXNIP and to characterize the expression patterns of each protein. RESULTS:TXN was a prognosis-related gene (P = 0.009), whereas TXNIP, a TXN inhibitor, demonstrated a negative correlation with TXN in the gene expression microarray data. In the 68 stage Ⅲ patients, the expression levels of both TXN and TXNIP had a statistically significant effect on recurrence-free survival (RFS, P = 0.008 and P = 0.036, respectively). The low TXN and high TXNIP expression group exhibited a better prognosis than the other groups, and the high TXN and low TXNIP expression group exhibited a poorer prognosis (P < 0.001 for RFS and P = 0.001 for overall survival). More than half of the patients in the simulta-neously high TXN and low TXNIP expression group ex- perienced a recurrence within 1 year after curative surgery, and the 5-year survival rate of the patients in this group was 29%, compared with 89% in the low TXN and high TXNIP expression group. The TXN protein was overexpressed in 65% of the gastric cancer tissues, whereas the TXNIP protein was underexpressed in 85% of the cancer cells. In a correlation analysis, TXN and TXNIP were highly correlated with many oncogenes and tumor suppressors as well as with genes related to energy, protein synthesis and autophagy. CONCLUSION:TXN and TXNIP are promising prognostic markers for gastric cancer, and performing personalized adjuvant treatment based on TXN and TXNIP expression levels would be an effective practice in the treatment of gastric cancer. | Jae Yun Lim Sun Och Yoo Soon Won Hong Jong Won Kim Seung Ho Choi Jae Yong Cho | 2012 | World Journal of Gastroenterology2012，18，39： | 4 |
| 9 | Rates and impact of hepatitis on human immunodeficiency virus infection in a large African cohort显示文摘AIM:To determine the rates and impact of hepatitis B virus(HBV) and hepatitis C virus(HCV) infections on response to long-term highly active antiretroviral therapy(HAART) in a large human immunodeficiency virus(HIV) population in Nigeria.METHODS:HBV and HCV as well as HIV infections are endemic in sub Saharan Africa.This was a retrospective cohort study of 19 408 adults who were recruited between June 2004 and December 2010 in the AIDS Prevention Initiative in Nigeria in Nigeria programme at Jos University Teaching Hospital.Serological assays,including HBV surface antigen(HBsAg) and hepatitis C antibody were used to categorise hepatitis status of the patients.HBsAg was determined using enzyme immunoassay(EIA)(Monolisa HBsAg Ultra3;Bio-Rad).HCV antibody was tested using third generation EIA(DIA.PRO Diagnostic,Bioprobes srl,Milan,Italy).HIV RNA levels were measured using Roche COBAS Amplicor HIV-1 monitor test version 1.5(Roche Diagnostics,GmbH,Mannheim,Germany) with a detection limit of 400 copies/mL.Flow cytometry was used to determine CD4+ cell count(Partec,GmbH Munster,Germany).Comparison of categorical and continuous variables were achieved using Pearson's χ 2 and Kruskal Wallis tests respectively,on MedCalc for Windows,version 9.5.0.0(MedCalc Software,Mariakerke,Belgium).RESULTS:With an overall hepatitis screening rate of over 90% for each virus;HBV,HCV and HBV/HCV were detected in 3162(17.8%),1983(11.3%) and 453(2.5%) HIV infected adults respectively.The rate of liver disease was low,but highest among HIV monoinfected patients(29,0.11%),followed by HBV coinfected patients(15,0.08%).Patients with HBV coinfection and triple infection had higher log 10 HIV RNA loads(HBV:4.6 copies/mL vs HIV only:4.5 copies/mL,P<0.0001) and more severe immune suppression(HBV:645,55.4%;HBV/HCV:97,56.7%) prior to initiation of HAART compared to HIV mono-infected patients(1852,48.6%)(P<0.0001).Of 3025 patients who were 4.4 years on HAART and whose CD4 cell counts results at baseline and end of follow up were available for analyses,CD4 increase was significantly lower in those with HBV co-infection(HBV:144 cells/mm3 ;HBV/HCV:105 cells/mm3) than in those with HCV co-infection(165 cells/mm3) and HIV mono-infection(150 cells/mm3)(P=0.0008).CONCLUSION:High rates of HBV and HCV infections were found in this HIV cohort.CD4 recovery was significantly diminished in patients with HBV co-infection. | Nimzing Gwamzhi Ladep Patricia Aladi Agaba Oche Agbaji Auwal Muazu Placid Ugoagwu Godwin Imade Graham Cooke Sheena McCormack Simon David Taylor-Robinson John Idoko Phyllis Kanki | 2013 | World Journal of Gastroenterology2013，19，10： | 3 |
| 10 | Diffuse large B-cell lymphoma with histone H3 trimethylation at lysine 27: another poor prognostic phenotype independent of c-Myc/Bcl2 coexpression显示文摘 | Eun Ji Oh Woo Ick Yang June-Won Cheong Sung-eun Choi Sun Och Yoon | 2014 | Human Pathology2014，，10： | 2 |
| 11 | The new proximal femoral nail antirotation (PFNA ? ) in daily practice: Results of a multicentre clinical study显示文摘 | R.K.J. Simmermacher J. Ljungqvist H. Bail T. Hockertz A.J.H. Vochteloo U. Ochs Chr.v.d. Werken | 2008 | Injury2008，，8： | 2 |
| 12 | Principles for retrofitting coal burners for oxy-combustion显示文摘 | Andrew Fry Brad Adams Alan Paschedag Paul Kazalski Casey Carney Danylo Oryshchyn Rigel Woodside Steve Gerdemann Thomas Ochs | 2011 | International Journal of Greenhouse Gas Control2011，，： | 2 |
| 13 | Scalpdex中文版的跨文化调适显示文摘目的对Scalpdex英文版进行跨文化调适,建立Scalpdex中文版,并对其信度和效度进行初步评价。方法对Scalpdex英文版进行正向翻译、综合协调、反向翻译、专家委员会评议,形成中文Scalpdex预试验版。使用预试验版对33例头皮银屑病患者进行预调查,进一步修订量表,建立正式中文版Scalpdex。通过访谈患者评价Scalpdex量表表面效度和内容效度,采用Cronbach’sα系数和分半信度评价内部一致性,Spearman相关分析检验聚合效度。结果 33例患者中,有25例(占75.8%)患者对所有条目完全理解;Scalpdex中文版的Cronbach’sα系数为0.94,分半信度为0.75。除条目19和20外,其余条目与所在维度的相关系数均>0.5,而与其他维度的相关系数则较弱。结论跨文化调适后的Scalpdex中文版适用于中国文化背景下头皮银屑病患者的生存质量评估。下一步可对中文版Scalpdex量表的测量性能进行大样本数据采集和评估。 | 夏萍 卢传坚 李艳 倪小佳 吴大嵘 Shelley Ochs Hunter Hu 杨波 欧爱华 闫玉红 赵芷睿 | 2016 | 现代预防医学2016，43，7： | 2 |
| 14 | TTF-1 mRNA-positive circulating tumor cells in the peripheral blood predict poor prognosis in surgically resected non-small cell lung cancer patients显示文摘 | Sun Och Yoon Young Tae Kim Kyeong Cheon Jung Yoon Kyung Jeon Baek-Hui Kim Chul-Woo Kim | 2010 | Lung Cancer2010，，2： | 2 |
| 15 | Automated tolerance analysis for mechanical assenblies modeled with geometric features and relational data structure 显示文摘 | P Treacy J B Ochs T M Ozsoy adn N Wang | 1991 | Computer-aided design1991，23，6： | 2 |
| 16 | Origin of mutations in two families with X-linked chronic granulomatous disease显示文摘 | Francke U Ochs HD Darras BT | 1990 | Blood1990，76，3： | 1 |
| 17 | Incorporation of gene ontology annotations to enhance microarray data analysis 显示文摘 | Ochs MF Peterson AJ Kossenkov A | 2007 | Methods Mol Biol2007，377，： | 1 |
| 18 | Glass substrate cleaning using a low energy ion source 显示文摘 | OCHS D SCHROEDER J | 2001 | Surface Coatings Technology2001，142，25： | 1 |
| 19 | The closer we look the more we see? Quantitative microscopic analysis of the pulmonary surfactant system显示文摘 | Ochs M | 2010 | Cell Physinl Biochem2010，25，1： | 1 |
| 20 | Chemical factors affecting the behaviour of fibers during papermaking显示文摘 | Tom Lindstroem Mo och Domsjoe AB | 1992 | Nordic Pulp and Paper Research Journal1992，，4： | 1 |
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