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| 1 | Translocation of annexin I from cellular membrane to the nuclear membrane in human esophageal squamous cell carcinoma显示文摘AIM: To investigate the alteration of the annexin I subcellular localization in esophageal squarnous cell carcinoma (ESCC)and the correlation between the translocation and the tumorigenesis of ESCC.METHODS: The protein localization of annexin I was detected in both human ESCC tissues and cell line via the indirect immunofluorescence strategy.RESULTS: In the normal esophageal epithelia the annex in I was mainly located on the plasma membrane and formed a consecutive typical trammels net. Annexin I protein also expressed dispersively in cytoplasm and the nuclei without specific localization on the nuclear membrane. In esophageal cancer annexin I decreased very sharply with scattered disappearance on the cellular membrane, however it translocated and highly expressed on the nuclear membrane,which was never found in normal esophageal epithelia. In cultured esophageal cancer cell line annexin I protein was also focused on the nuclear membrane, which was consistent with the result from esophageal cancer tissues.CONCLUSION: This observation suggests that the translocation of annexin I protein in ESCC may correlate with the tumorigenesis of the esophageal cancer. | YuLiu Hui-XinWang NingLu You-ShengMao FangLiu YingWang Hai-RongZhang KunWang MinWu Xiao-HangZhao | 2003 | World Journal of Gastroenterology2003，9，4： | 10 |
| 2 | Alterations in expression,proteolysis and intracellular localizations of clusterin in esophageal squamous cell carcinoma显示文摘AIM: To investigate biogenesis and intracellular Iocalizations of clusterin to elucidate the potential molecular mechanisms implicated in tumorigenesis of esophageal mucosa.METHODS: Semi-quantitative RT-PCR for multi-region alteration analysis, Western blot for different transcriptional forms and immunohistochemical staining for intracellular Iocalizations of clusterin were carried out in both tissues and cell lines of ESCC.RESULTS: The N-terminal deletions of the clusterin gene and the appearance of a 50-53 ku nuclear clusterin, an uncleaved, nonglycosylated, and disulfide-linked isoform,were the major alterations in cancer cells of esophagus.Naturally the 40 ku clusterin was located in the connective tissue of the lamina propria of epithelial mucosa and right under the basal membrane of epithelia, but it was disappeared in stromal mucosa of esophagus and the pre-matured clusterin was found positive in cancerous epithelia.CONCLUSION: The N-terminal deletion of clusterin may be essential for its alterations of biogenesis in ESCC. | Hong-ZhiHe Zhen-MeiSong KunWang Liang-HongTeng FangLiu You-ShengMao NingLu Shang-ZhongZhang MinWu Xiao-HangZhao | 2004 | World Journal of Gastroenterology2004，10，10： | 7 |
| 3 | GLB prevents tumor metastasis of Lewis lung carcinoma by inhibiting tumor adhesion actions显示文摘Aim: To investigate the inhibitory effect of a new compound of GLB on tumor metastasis in vivo and analyze its actions on tumor cell adhesion to clarify its mechanism. Methods: The effect of GLB on tumor metastasis was analyzed by Lewis lung carcinoma model. The pathological morphology of lung alveolar was evaluated by hematoxylin-eosin staining. The effect of GLB on the proliferation of human prostate cancer cell (PC-3M, with a high metastatic characteristic) was studied using the Mq-T method, and its actions on PC-3M cell adhesion to human umbilical vein endothelial cells (HUVEC) and laminin were analyzed in vitro. Results: GLB (100 mg·kg^-1·d^-1 for 28 d, ig) reduced the number of lung colonies of Lewis lung carcinoma metastasis significantly (P<0.05). Simultaneously, GLB could mitigate the damage of lung alveolar caused by metastasic tumor deposits. In vitro, GLB inhibited dramatically the adhesion of PC-3M cells to HUVEC (P<0.01) and laminin (P<0.05), without cytotoxic or anti-proliferative action on PC-3M cells. Conclusion: GLB has anti-tumor metastatic activity, which partly depends on its inhibition of tumor adhesion. | YanPAN Qian-liuSONG Yan-huaLIN NingLU He-ruingYU XueojunLI | 2005 | Acta Pharmacologica Sinica2005，26，7： | 4 |
| 4 | Ex- perimental study of novel Co-based catalysts for tar model compound cracking显示文摘 | TANG Ninglu WU Zhengshun LIU Xiaoyan | 2012 | Journal of Biobased Materials and Bioenergy2012，6，1234567： | 1 |
| 5 | Down-regulation of γ-synuclein in human esophageal squamous cell carcinoma显示文摘AIM: To investigate gene expression pattern of human γsynuclein gene in human esophageal squamous cell carcinoma (ESCC) by using semi-quantitive reverse transcription polymerase chain reaction (RT-PCR), and to study the role of γ-synudein in the development of human ESCC.METHODS: Semi-quantitive RT-PCR of 27 pairs of specimens of human ESCC tissues and corresponding normal tissues was used to investigate the expression pattern of γsynuclein in ESCC. 9706/γ-syn cells in which γ-synuclein was overexpressed were obtained through cloning γ-synuclein gene by PCR and transfecting it into ESCC 9706 cells, then selecting with G-418 for 14 days. The biological effects of γsynuclein were measured and compared between 9706/γ-syn and 9706/vec cells by cell growth curve and soft agar assay.RESULTS: RT-PCR showed that γ-synuclein gene was expressed in all the 27 cases of normal epithelial tissues,while downregulation of γ-synudein was observed in 16 out of the 27 cases (59.3%) of ESCC. There were also 6 cases of ESCC tissues with a high expression level of γ-synuclein mRNA. In functional analysis we found that over-expression of γ-synuclein in ESCC 9706 cells could inhibit the growth rate and transformation ability of ESCC 9706 cells.CONCLUSION: The low expression level of γ-synuclein in human ESCC and the biological effects of γ-synuclein overexpression on ESCC 9706 cells suggest that γ-synuclein may play a role as a negative regulator in the development of human ESCC. | Cui-QiZhou ShuangLiu Li-YanXue Yi-HuaWang Hong-XiaZhu NingLu Ning-ZhiXu | 2003 | World Journal of Gastroenterology2003，9，9： | 1 |
| 6 | Cell density mediated pericellular hypoxia and COCl2 induced hypoxia leads to AQPI induction under isotonic conditions in vitro显示文摘Recently, many reports have linked aquaporin 1 (AQPI)protein overexpression with tumorigenesis, tumor growth and progression, but the underlying mechanisms on regulating of AQP1 protein expression are still not clear. We hypothesized that hypoxia might play an important role in AQP1 induction during tumorigenesis as well as at the late stages of tumor development. To prove this hypothesis, we studied the | NingLU YanPAN Yan-huaLIN Jun-weiGAO Tian-luoLEI He-mingYU Xue-junLI | 2004 | Acta Pharmacologica Sinica2004，25，11： | 0 |
| 7 | Clinical Features and Mid-Term and Long-Term Outcomes of Surgical Treatment of 8 Patients with Primary Ventricular Tumors显示文摘OBJECTIVE To summarize the clinical features and surgical treatment of primary ventricular tumors. METHODS Eight patients with primary ventricular tumor, aged 3 to 52 years, underwent surgical treatment. There were 6 males and 2 females. The pathological diagnoses were as follows: multiple left ventricular myxomas in 2 cases; left ventricular rhabclomyoma, fibroma and malignant neurolemmoma in 1 case for each; right ventricular myxoma and malignant neurolemmoma in 1 case for each; intraseptal fibroma in 1 case. The operations were performed through median sternotomy with moderate hypothermic cardiopulmonary bypass in 7 cases; via left anterolateral thoracotomy without extracorporeal circulation in 1 case. Tumors were totally removed in 7 cases and subtotally resectecl in 1 case. RESULTS Cardiac arrest after anaesthetization occurred in 1 case with postoperative coma for 10 days. One case died of massive gastro-intestinal hemorrhage postoperatively. Seven cases survived, During a follow-up period of 1 to 21 years, there was no recurrence or metastasis in the 6 cases who received complete tumor resection including 2 cases with malignant tumor. One case of partial tumor removal had a mild heart murmur without tumor progression. All patients were asymptomatic with cardiac functiongrade I. CONCLUSION Primary ventricular tumors showed diversity in their histological characteristics. The mid- and long-term outcomes of surgical treatment for primary ventricular tumors appear to be satisfactory. | JianminYao QingrenJia QirenCheng NingLu XiangdongZhao ZhibinXiao XiaomengZhang | 2004 | Chinese Journal of Clinical Oncology2004，1，2： | 0 |
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