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| 1 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 2 | Feasibility of robotic assisted bladder sparing pelvic exenteration for locally advanced rectal cancer:A single institution case series显示文摘BACKGROUND Pelvic exenteration for locally advanced rectal cancer involving prostate has been performed via open surgery.Robotic pelvic exenteration offers benefits of better pelvic visualisation and dissection for bladder preserving prostatectomy with vesicourethral anastomosis,while achieving clear margins.AIM To determine the feasibility of robotic assisted bladder sparing pelvic exenteration.METHODS We describe robotic assisted pelvic exenteration in three cases of locally advanced rectal cancer involving prostate and seminal vesicles(SV).The da Vinci S robotic system was used.Robotic console was docked at left oblique position for abdominal phase and redocked to between the patient’s legs for pelvic phase.All three cases were performed fully robotically at Tan Tock Seng Hospital by colorectal and urological teams.RESULTS Case 1:67-year-old with low rectal tumour 3 cm from anal verge involving the prostate.He underwent neo-adjuvant chemoradiotherapy and robotic abdominoperineal resection with en-bloc prostatectomy.Case 2:66-year-old with low rectal tumour 3 cm from anal verge involving prostate and bilateral SV.He underwent neo-adjuvant chemoradiotherapy and robot assisted ultra-low anterior resection with coloanal anastomosis and en-bloc prostatectomy.Case 3:57-year-old with metachronous rectal tumour in the rectovesical pouch inseparable from the anterior mid rectum,prostate and bilateral SV.He underwent robot assisted ultra-low anterior resection with en-bloc prostatectomy.Bladder neck margin revealed cauterized tumour cells,and he underwent total cystectomy and ileal conduit creation.Histology revealed no residual tumour.All patients are currently disease free CONCLUSION Robot assisted bladder sparing pelvic exenteration can be safely performed in locally advanced rectal cancer with acceptable surgical outcome while preserving benefits of minimally invasive surgery. | Nathaniel H Heah Kar Yong Wong | 2020 | World Journal of Gastrointestinal Surgery2020，12，4： | 2 |
| 3 | Economic Development through Bureaucratic Corruption 显示文摘 | Leff Nathaniel H | 1964 | The American Behavioural Scientists1964，，33： | 1 |
| 4 | An Octahedral Template Based on a New Molecular Turn:Synthesis and Structure of a Model Complex and a Reactive,Diphenolic Ligand and Its Metal Complexes显示文摘 | Andrew L V Nathaniel W A Joseph A H | 1998 | Inorg Chem1998，37，： | 1 |
| 5 | Uniaxial cyclicstress-strain behavior of structural steel 显示文摘 | Nathaniel G C Krawinkler H | 1985 | Journal ofEngineering Mechanics1985，111，9： | 1 |
| 6 | ,1969,Depondeney Rates and Savings Rate 显示文摘 | Leff Nathaniel H | 1969 | American Economic Raview1969，59，5： | 1 |
| 7 | Cerebral metabolic correlates as potential predictors of response to anterior cingulotomy for obsessive compulsive disorder显示文摘 | Scott L Rauch Darin D Dougherty G.Rees Cosgrove Edwin H Cassem Nathaniel M Alpert Bruce H Price Andrew A Nierenberg Helen S Mayberg Lee Baer Michael A Jenike Alan J Fischman | 2001 | Biological Psychiatry2001，，9： | 1 |
| 8 | lnterferometric measurements of femtosecond group delay in optical components 显示文摘 | WAYNE H K NATHANIEL M P KATHRYN D L | 1988 | Opt Lett1988，13，7： | 1 |
| 9 | Augmented measurement system assess- ment 显示文摘 | Nathaniel T Stevens Ryan Browne Stefan H Steiner etc | 2010 | Journal of Quality Technology2010，42，4： | 1 |
| 10 | Industrial Organization and Entrepreneurship in the Developing Countries:The Economic Groups显示文摘 | Leff Nathaniel H | 1978 | Economic Development and Cultural Changes1978，26，4： | 1 |
| 11 | Dependency Rates and Savings Rate显示文摘 | Leff Nathaniel H | | 0，，05： | 1 |
| 12 | Autophagy and its possible roles in nervous system diseases,damage and repair显示文摘 | Rubinsztein DC Marian D Nathaniel H | | 0，，1： | 1 |
| 13 | , Economic Development thro- ugh Bureaucratic Corruption显示文摘 | Left Nathaniel H | 1964 | American Behavioral Sci- entist1964，8，3： | 1 |
| 14 | Economic Development Through Bureaucratic Corruption显示文摘 | Left Nathaniel H | 1964 | The American Behavioral Scientist1964，，8： | 1 |
| 15 | Dependency Rates and Sav- ings Rates显示文摘 | Nathaniel H Left | 1969 | American Economic Review Vol 59 No 5 (Dec1969，，： | 1 |
| 16 | Dependency Rates and Savings Rates显示文摘 | Left Nathaniel H | 1969 | American Economic Review1969，59，5： | 1 |
| 17 | Methods for estimating the optimal dividend barrier and the probability of ruin Insurance显示文摘 | GERBER H U SHIU E S W NATHANIEL S | 2008 | Mathematics and Economics2008，42，2： | 1 |
| 18 | Dependency Rates and Savings Rate 显示文摘 | Leff Nathaniel H | 1969 | American Economic Review1969，，59： | 1 |
| 19 | An Evaluation of Radar Texture for Land Use/Cover Extraction in Varied Landscapes显示文摘 | Nathaniel D H Barry N H Elizabeth S | 2004 | International Journal of Applied Earth Observation and Geoinformation2004，，5： | 1 |
| 20 | Optimal dividends with incomplete information in the dual model 显示文摘 | GERBER H U NATHANIEL S | 2008 | Insurance: Mathematics and Economics2008，43，2： | 1 |
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