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| 1 | Establishment of hepatocellular carcinoma multidrug resistant monoclone cell line HepG2/mdr1显示文摘multidrug 抵抗(MDR ) 与 mdr1 基因和它的产品 P-glycoprotein 的表示联系了的背景是在癌房间(HCC ) 病人与化疗对待的 hepatocellular 的预后的一个主要因素。我们的学习是建立明确地,与转基因的 mdr1.Methods 的 overexpression 有关, 4.5-kb mdr1 cDNA 从 plasmid pHaMDR1-1 获得了的 multidrug 抵抗的 de novo 获得被克隆进 PCI-neo 哺乳动物的表示向量的一根稳定的 HCC MDR 房间线,后来被 liposome 转移到人的 hepatocarcinoma 房间线 HepG2 。然后,抵抗 G418 的 transfected HepG2 房间被聚类并且有教养并且在这些 HepG2 房间的 mdr1 cDNA, mRNA 和 P-glycoprotein (Pgp ) 的特定的碎片被 PCR, RT-PCR 和流动 cytometry 分别地检测。daunorubicin 的累积同时是由流动 cytometry 的 determinated。grafting 瘤的裸体老鼠模特儿被皮下地在正确腋下注射 HepG2/mdr1 房间建立。当瘤直径到达了 5 公里时, adriamycin 被注入腹洞。瘤的尺寸和生长抑制是 evaluated.Results mdr1 表示向量成功地被构造, MDR HCC 线 HepG2/mdr1 developed.The PCR 分析证明 mdr1 的特定的碎片在 HepG2/mdr1 的 cDNA 房间,然而并非在控制组 HepG2 房间。而且,在 HepG2/mdr1 房间的 mdr1 mRNA 和 Pgp 表示的特定的碎片的内容是(59. | CHEN Yong-bing YAN Mao-lin GONG Jian-ping XIA Ren-pin LIU Li-xin LI Ning LU Shi-chun ZHANG Jing-guang ZENG Dao-bing XIE Jian-guo YANG Jia-yin YAN Lü-nan | 2007 | Chinese Medical Journal2007，，8： | 16 |
| 2 | 中国道路交通死亡率:2006—2016年国家疾病监测数据分析显示文摘可持续发展目标(SDG)3.6旨在2020年将全球道路交通事故伤亡人数减半。本研究目的在于评价中国2006—2016年降低道路交通伤害死亡率的进展。采用国家死因监测系统数据,用标准误计算粗率和年龄标准化死亡率,采用断点回归分析评价和量化2006—2016年整体和亚组道路交通死亡率的趋势。 | Wang L Ning P Yin P 李若曈(编译) 胡国清(编译) | 2019 | 中华预防医学杂志2019，53，9： | 15 |
| 3 | Periodicity and map for discovery of new ionic liquids显示文摘There is virtually no limit in the number of ionic liquids. How to select proper ones or discover new ones with desirable properties in such a large pool of ionic liquids? It has become a bottleneck in the researches and applications of ionic liquids. Mendeleev’s periodic law states that the properties of the elements vary periodically. Whether the similar regularity exists among ionic or mo-lecular fragments of compounds is an interesting topic. In this work, we attempted to establish a pe-riodicity and draw a “map” of ionic liquids for providing definite guidance to discover, design, and se-lect the proper ionic liquids rather than trial-and-error. If a complete regularity of the system of ionic liquids can be finally established in the future, we are near an epoch in understanding the existing differences and the reasons for the similarity of the ions or molecular fragments. | ZHANG Suojiang, SUN Ning, ZHANG Xiangping & Lü Xingmei Research Laboratory for Green Chemical Engineering and Technology, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China Correspondence should be addressed to Zhang Suojiang | 2006 | Science China Chemistry2006，49，2： | 9 |
| 4 | Further Recognition of Petroleum Exploration Potential of Marine Carbonates in Western Tarim Basin显示文摘A series of significant discoveries in marine carbonate rocks show great petroleum exploration potential in the Tarim Basin. However, the oil and gas fields discovered in the carbonate rocks are mainly distributed around the Manjiaer Sag in the eastern Tarim Basin. Some explorations occurred and no oil or gas field was discovered around the Awati Sag in the western Tarim Basin. Information from wells and outcrops reveals that there are excellent oil and gas source rock conditions around the Awati Sag. Transformed reef-shoal reservoirs could be formed in the Ordovician carbonate rocks with paleo-geographic background and hydrothermal conditions. Therefore, it is necessary to make a systematical study and overall evaluation of the potential of the periphery of the Awati Sag in terms of source rock evolution, resource potential, high-grade reservoir formation and distribution, and main factors controlling hydrocarbon migration and accumulation. | Lü Xiuxiang Yang Haijun Yang Ning Zhao Fengyun Ma Yujie | 2007 | Petroleum Science2007，4，3： | 8 |
| 5 | The association between the genetic polymorphisms of LMP2/LMP7 and the outcomes of HCV infection among drug users显示文摘Objective:To investigate a possible association of LMP2/LMP7 genes with chronic hepatitis C virus(HCV) infection,and to assess whether LMP2/LMP7 genes could influence the outcomes of HCV infection among drug users.Methods:Genomic DNAs of 362 anti-HCV sero-positive drug users and 225 control drug users were extracted from the peripheral blood leukocytes.The sero-positive patients were divided into those who had persistent infection and those who had spontaneously cleared the infection.Polymorphisms of LMP genes were determined by PCR combined with restriction fragment length polymorphism(RFLP).Results:The distribution of LMP2 genotypes among the control,persistent infection and spontaneous clearance groups were not different.However,the LMP7 codon 145 Gln/Lys,Lys/Lys,and Gln/Lys+Lys/Lys genotypes were found significantly more frequent in the persistent infection group than in control group(OR=1.75,95%CI=1.06~2.90;OR=3.16,95%CI=1.23-8.12;OR=1.94,95%CI=1.21-3.12,respectively).Similarly,the frequencies of the codon 145 Gln/Lys,Lys/Lys,and Gln/Lys+Lys/Lys genotypes were found significantly more frequent in the persistent infection group than in the spontaneous clearance group(OR=1.64,95%CI=1.04-2.57;OR=2.40,95%CI=1.09-5.28;OR=1.76,95%CI=1.152.69,respectively).Stratified analysis indicated that combined genotype Gln/Lys+Lys/Lys of the LMP7 gene was related to an increasing susceptibility to HCV infection(OR=1.91,95%CI=1.02-3.55;OR=2.19,95%CI=1.243.89;OR=1.91,95%CI=1.05-3.48,OR=2.86,95%CI=1.41-5.78,respectively)and the risk of persistent HCV infection(OR=1.94,95%CI=1.12-3.34;OR=2.02,95%CI=1.21-3.38;OR=1.78,95%CI=1.12-2.85,OR=2.23,95%CI=1.09-4.58,respectively)among>30-year-old,males,the injection drug user(IDU)subjects and/or the shorter duration drug users(≤5 y).Conclusion:These results suggest that polymorphism of the LMP7 gene may have an influence on the outcomes of HCV infection,and is one of the factors accounting for the genetic susceptibility to HCV infection among drug users. | Qian Cui Yongxiang Zhang Jing Su Chao Shi Na Lei Keqin Ding Jun Li Rongbin Yu L u Wang Ning Wang | 2010 | The Journal of Biomedical Research2010，24，5： | 6 |
| 6 | Mechanokinetics of receptor-ligand interactions in cell adhesion显示文摘Receptor-ligand interactions in blood flow are crucial to initiate such biological processes as inflammatory cascade,platelet thrombosis,as well as tumor metastasis.To mediate cell adhesion,the interacting receptors and ligands must be anchored onto two apposing surfaces of two cells or a cell and a substratum,i.e.,two-dimensional(2D)binding,which is different from the binding of a soluble ligand in fluid phase to a receptor,i.e.,three-dimensional(3D) binding.While numerous works have been focused on3 D kinetics of receptor-ligand interactions in the immune system,2D kinetics and its regulations have been less understood,since no theoretical framework or experimental assays were established until 1993.Not only does the molecular structure dominate 2D binding kinetics,but the shear force in blood flow also regulates cell adhesion mediated by interacting receptors and ligands.Here,we provide an overview of current progress in 2D binding and regulations,mainly from our group.Relevant issues of theoretical frameworks,experimental measurements,kinetic rates and binding affinities,and force regulations are discussed. | Ning Li Shouqin Lü Yan Zhang Mian Long | 2015 | Acta Mechanica Sinica2015，31，2： | 5 |
| 7 | Experimental study of natural hydroxyapatite/chitosan composite on reconstructing bone defects显示文摘Objective:To study the possibility of natural hydroxyapatite/chitosan composite on repairing bone defects. Methods:We developed a natural hydroxyapatite/chitosan composite that could be molded into any desired shape. The powder component consists of natural hydroxyapatite, which is epurated from bone of pigs. The liquid component consists of malic acid and chitosan. Operations were performed on the left tibias of 15 white rabbits to create two square bone defects. One of the defects was reconstructed with the composite, while the other was not repaired and used as a blank control. Three of the animals were killed at the end of 2 weeks, 4 weeks, 8 weeks, 12 weeks and 16 weeks respectively and implants were evaluated anatomically and histologically. Results:No apparent rejection reaction was found, except for a mild inflammatory infiltration observed 2 weeks after surgery. Fibrous tissue became thinner 2~8 weeks after surgery and bony connections were detected 12 weeks after surgery. The new bone was the same as the recipient bone by the 16th postoperative week. Conclusion:The hydroxyapatite/chitosan composite has good biocompatibility and osteoconduction. It is a potential repairing material for clinical application. | Hua Yuan Ning Chen Xiaoying Lü, Buzhong Zheng | 2008 | Journal of Nanjing Medical University2008，22，6： | 5 |
| 8 | Biliary papillomatosis: analysis of 18 cases显示文摘 | JIANG Li YAN Lü-nan JIANG Li-sheng LI Fu-yu YE Hui LI Ning CHENG Nan-sheng ZHOU Yong | 2008 | Chinese Medical Journal2008，，24： | 4 |
| 9 | Antrodia Cinnamomea ameliorates neointimal formation by inhibiting infl ammatory cell infi ltration through downregulation of adhesion molecule expression in vitro and in vivo显示文摘The increased vascular infl ammation is a key event in the development of atherosclerotic lesions.Antrodia cinnamomea has been shown to promote anticancerogenic activity through decreasing infl ammation.However,the potential role of A.cinnamomea in cardiovascular diseases remains unexplored.Herein,using carotid arterial ligation models,we found that ethanol extract from A.cinnamomea(EEAC)signifi cantly inhibited neointimal hyperplasia in a dose-dependent manner,accompanied with the reduced expression of activated p65 and infl ammatory cytokines.We also show that EEAC ameliorated TNF-α-induced phosphorylation of p65 and pro-infl ammatory cytokine expression in both vascular smooth muscle cells(VSMCs)and macrophages in vitro.Mechanistically,EEAC suppressed expression levels of intercellular adhesion molecule-1(ICAM-1)and vascular cell adhesion molecule(VCAM-1)in VSMCs,which attenuates the ability of monocytes/macrophages adhesion to VSMCs.Furthermore,the expression level of these adhesion molecules and infi ltration of monocytes/macrophages were also decreased in neointimal VSMCs of arteries pretreated with EEAC.Altogether,our results reveal a novel function of A.cinnamomea in suppressing vascular infl ammation upon ligation injury during neointimal formation,likely through inhibition of infl ammatory cell infi ltration via downregulating the adhesion molecules in VSMCs.Thus,A.cinnamomea may offer a pharmacological therapy to slow down disease progression in patients with vascular injury. | Yan Zhang Aijin Ma Hao Xi Ning Chen Rong Wang Chenhui Yang Jinbang Chen Pin Lü Fuping Zheng Wenyi Kang | 2021 | Food Science and Human Wellness2021，10，4： | 4 |
| 10 | Numerical simulation of flow focusing pattern and morphological changes in two-phase flow inside nozzle显示文摘The flow focusing nozzle is a new type of nozzle that performs effective atomization of the discrete phase by means of high-speed motion of the continuous phase.The flow pattern and its morphological changes have a significant effect on the atomization, but the influence of different parameters on the morphological change of the flow pattern remains unclear.The flow focusing pattern and morphological changes in the two-phase flow inside the nozzle were simulated numerically, based on the volume of fluid method.The results demonstrate that the ratio of the nozzle-to-capillary distance and capillary diameter, the gas–liquid velocity ratio, and capillary diameter have significant effects on the flow pattern.When the ratio of the nozzle-to-capillary distance H and capillary diameter D increases, or the capillary diameter D increases, the flow pattern tends to transform into a laminar form; however, when the gas–liquid velocity ratio V increases, the flow pattern tends to transform into a turbulence form.Furthermore, we define the cone-shaped expansion rate, cone-shaped focusing rate,and cone angle in order to study the morphological changes in the cone shape inside the nozzle.The results indicate that the morphological change of the cone shape and flow pattern transformation is interrelated.When the cone shape tends to be unstable, the flow pattern changes towards flow blurring, whereas, a stable cone indicates that the flow tends to exhibit a droplet pattern. | Jin Zhao Zhi Ning Ming Lü Geng Wang | 2019 | Chinese Journal of Chemical Engineering2019，27，1： | 2 |
| 11 | Numerical analysis of the 2D acoustic field with fuzzy-random parameters显示文摘Regard for the fuzziness and the randomness in some acoustic fields,a method for the numerical analysis of the 2D acoustic field with Fuzzy-Random parameters was proposed based on the equivalent conversion of information entropy.In the proposed method,a fuzzyrandom acoustic field was treated as a pure fuzzy acoustic field or a pure random acoustic field by transforming all the variables into fuzzy variables or random variables.Perturbation finite element methods for analyzing the two-dimensional acoustic fuzzy and random field are deduced.The sound pressure response of a 2D acoustic tube and the 2D acoustic cavity of a car with fuzzy-random parameters were analyzed by the proposed method and the Monte Carlo method,the results show that the proposed method can be well applied to the numerical analysis of the 2D acoustic field with fuzzy-random parameters,and has good prospect of engineering application. | CHEN Ning YU Dejie XIA Baizhan L Hui | 2014 | Chinese Journal of Acoustics2014，33，4： | 2 |
| 12 | Breakup of Cavitation Bubbles within the Diesel Droplet显示文摘Supercavitation in the diesel nozzle increases the instability of droplets in part due to the two-phase mixture,while the effect of cavitation bubbles on the instability of drops is still unclear.In order to investigate the breakup of cavitation bubbles within the diesel droplet,a new mathematical model describing the disturbance growth rate of the diesel bubble instability is developed.The new mathematical model is applied to predict the effects of fluids viscosity on the stability of cavitation bubbles.The predicted values reveal that the comprehensive effect of fluids viscosity makes cavitation bubbles more stable.Compared with the viscosities of air and cavitation bubble,the diesel droplet’s viscosity plays a dominant role on the stability of cavitation bubbles.Furthermore,based on the modified bubble breakup criterion,the effects of bubble growth speed,sound speed,droplet viscosity,droplet density,and bubble-droplet radius ratio on the breakup time and the breakup radius of cavitation bubbles are studied respectively.It is found that a bubble with large bubble-droplet radius ratio has the initial condition for breaking easily.For a given bubble-droplet radius ratio(0.2),as the bubble growth speed increases(from 2 m/s to 60 m/s),the bubble breakup time decreases(from 3.59μs to 0.17μs)rapidly.Both the greater diesel droplet viscosity and the greater diesel droplet density result in the increase of the breakup time.With increasing initial bubble-droplet radius ratio(from 0.2 to 0.8),the bubble breakup radius decreases(from 8.86μm to 6.23μm).There is a limited breakup radius for a bubble with a certain initial bubble-droplet radius ratio.The mathematical model and the modified bubble breakup criterion are helpful to improve the study on the breakup mechanism of the secondary diesel droplet under the condition of supercavitation. | L Ming NING Zhi YAN Kai FU Juan SONG Yunchao SUN Chunhua | 2014 | Chinese Journal of Mechanical Engineering2014，27，1： | 2 |
| 13 | Oxidative Stress and Matrix Metalloproteinase-9 in Acute Ischemic Stroke: The Biomarker Evaluation for Antioxidant Therapies in Stroke (BEAT-Stroke) Study显示文摘 | Peter J. Kelly Jason D. Morrow MingMing Ning Walter Koroshetz Eng H. Lo Erin Terry Ginger L. Milne Jane Hubbard Hang Lee Elizabeth Stevenson Megan Lederer Karen L Furie | 2008 | Stroke2008，，1： | 2 |
| 14 | Fabrication of poly ( DL-lactic acid ) / wollastonite composite films with surface modified β -CaSiO3 particles 显示文摘 | Ye L Z Chang J Ning C Q | 2008 | J Biomater Appl2008，22，： | 1 |
| 15 | Mammalian Apoptosis-Assoiated Genes c-myc and p53 in Maize: Homologs and Their Locations显示文摘 | Ning S B Song Y C Wang L | 2000 | International J of Cytology(Cytologia)2000，65，3： | 1 |
| 16 | Multichannel AR spectrum estimation:the optimum approach in the reflection coefficient domain显示文摘 | T Ning C L Nikias | 1986 | IEEE Transaction on Acoustics Speech and Signal Processing1986，34，5： | 1 |
| 17 | Bone marrow cells stained by azide-conjugated Alexa fluors in the absence of an alkyne label 显示文摘 | Lin G Ning H Banie L | 2012 | Stem Cells Dev2012，21，： | 1 |
| 18 | A mechanical calculation of the flexible & floating anti-ship collision device for bridge piers显示文摘 | Lti W Lu R L Ning X L | 2012 | Advanced Materials Research2012，，： | 1 |
| 19 | Reductive decomposition of phosphogypsum with high-sulfur-concentration coal to SO2 in an inert atmosphere 显示文摘 | Zheng S C Ning P Ma L P | 2011 | Chemical Engineering Research and Design2011，89，： | 1 |
| 20 | Surface characterization studies of CuO-CeO2-ZrO2catalysts for selective catalytic reduction ofNO with NH3 显示文摘 | Zhang Q L Xu L S Ning P | 2014 | Applied Surface Science2014，317，： | 1 |
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