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| 1 | A community-derived classification for extant lycophytes and ferns显示文摘发展史长通知了蕨类植物分类。当我们推断进化的树的能力改善了,针对认出生来的组的分类变得逐渐地预兆、稳定。这里,我们为 lycophytes 和蕨纲植物提供一个现代、全面分类,在下面,利用一条基于社区的途径类水平。我们 monophyly 用作主要标准让 taxa,而且目的识别保存两个广泛地被接受的存在 taxa 和界限并且与我们蕨类植物发展史的理解一致。总共,这个分类对待一在 337 个类, 51 个家庭, 14 目,和二个班估计了 11 916 种类。这个分类没在 lycophyte 和蕨纲植物上作为最后的词被打算分类,而是当前的假设的概括陈述,源于最好的可得到的数据并且在问题由熟悉植物的那些大多数塑造了。我们希望它将在蕨类植物上为最近的文学的那些想要的参考用作一个资源发展史和分类,为指导未来调查的一个框架,和推进讲话的刺激。 | Eric Schuettpelz Harald Schneider Alan R. Smith Peter Hovenkamp Jefferson Prado Germinal Rouhan Alexandre Salino Michael Sundue Thafs Elias Almeida Barbara Parris Emily B. Sessa Ashley R. Field Andre Luis de Gasper Carl J. Rothfels Michael D. Windham Marcus Lehnert Benjamin Dauphin Atsushi Ebihara Samuli Lehtonen Pedro Bond Schwartsburd Jordan Metzgar Li-Bing Zhang Li-Yaung Kuo Patrick J. Brownsey Masahiro Kato Marcelo Daniel Arana Francine C. Assis Michael S. Barker David S. Barrington Ho-Ming Chang Yi-Han Chang Yi-Shan Chao Cheng-Wei Chen De-Kui Chen Wen-Liang Chiou Vinicius Antonio de Oliveira Dittrich Yi-Fan Duan Jean-Yves Dubuisson Donald R. Farrar Susan Fawcett Jose Maria Gabriel y Galan Luiz Armando de Araujo Goes-Neto Jason R. Grant Amanda L. Grusz Christopher Haufler Warren Hauk Hai He Sabine Hennequin Regina Yoshie Hirai Layne Huiet Michael Kessler Petra Korall Paulo H. Labiak Anders Larsson Blanca Leen Chun-Xiang Li Fay-Wei Li Melanie Link-Perez Hong-Mei Liu Ngan Thi Lu Esteban I. Meza-Torres Xin-Yuan Miao Robbin Moran Claudine Massi Mynssens Nathalie Nagalingum Benjamin Ollgaard Alison M. Paul Jovani B. de S. Pereira Leon R. Perrie Monica Ponce Tom A. Ranker Christian Schulz Wataru Shinohara Alexander Shmakov Erin M. Sigel Filipe Soares de Souza Lana da Silva Sylvestre Weston Testo Luz Amparo Triana-Moreno Chie Tsutsumi Hanna Tuomisto IvAn A. Valdespino Alejandra Vasco Raquel Stauffer Viveros Alan Weakley Ran Wei Stina Weststrand Paul G. Wolf George Yatskievych Xiao-Gang Xu Yue-Hong Yan Liang Zhang Xian-Chun Zhang Xin-Mao Zhou | 2016 | Journal of Systematics and Evolution2016，54，6： | 44 |
| 2 | Obesity leads to higher risk of sperm DNA damage in infertile patients显示文摘在在富饶上的男营养的影响的过去几年有成长兴趣。不孕被连接了到男过重或肥胖,并且常规精液参数值似乎在高身体团索引(BMI ) 的情况下被改变。在精子 DNA 正直上估计 BMI 的影响的一些研究被出版了,但是他们没导致强壮的一致。我们的目的是进一步探索在精子 DNA 正直和 BMI 之间的关系,通过 3 年的 multicentre 研究。在 subfertile 夫妇的 330 个男搭挡被包括。用标记的终端 uridine 刻痕结束(TUNEL ) 试金,我们在肥胖的人观察了精子 DNA 损坏的增加的率(机会比率(95 %信心间隔) :2.5 (1.2-5.1 )) 。 | Charlotte Dupont Celine Fauret Nathalie Sermondade Marouane Boubaya Florence Eustache Patrice Clement Pascal Briot Isabelle Berthaut Vincent Levy Isabelle Cedrin-Durnerin Brigitte Benzacken Pascale Chavatte-Palmer Rachel Levy | 2013 | Asian Journal of Andrology2013，15，5： | 27 |
| 3 | Froth flotation of scheelite – A review显示文摘Froth flotation of scheelite has regained new focus since the 2010s and research regarding floatability and reagents has made great progress over the years. The main objective was and remains the selective flotation of scheelite from other calcium-bearing minerals, in particular calcite, fluorite and apatite. Due to similar properties, most attempts have limited success or only specific application(linked to a type of ore or a location). This article aims at reviewing all general physical-chemical information on froth flotation of scheelite, including electrokinetic properties, influence of p H and already existing reagents as well as ones still under examination. It appears that chelating or mixed collectors and modified versions of sodium silicate and quebracho hold great promise for scheelite flotation, while the use of said depressants and/or promoters seems inevitable. | Nathalie Kupka Martin Rudolph | 2018 | International Journal of Mining Science and Technology2018，28，3： | 21 |
| 4 | Non-alcoholic fatty liver disease and diabetes: From physiopathological interplay to diagnosis and treatment显示文摘Non-alcoholic fatty liver disease(NAFLD)is highly prevalent in patients with diabetes mellitus and increasing evidence suggests that patients with type 2diabetes are at a particularly high risk for developing the progressive forms of NAFLD,non-alcoholic steatohepatitis and associated advanced liver fibrosis.Moreover,diabetes is an independent risk factor for NAFLD progression,and for hepatocellular carcinoma development and liver-related mortality in prospective studies.Notwithstanding,patients with NAFLD have an elevated prevalence of prediabetes.Recent studies have shown that NAFLD presence predicts the development of type2 diabetes.Diabetes and NAFLD have mutual pathogenetic mechanisms and it is possible that genetic and environmental factors interact with metabolic derangements to accelerate NAFLD progression in diabetic patients.The diagnosis of the more advanced stages of NAFLD in diabetic patients shares the same challenges as in non-diabetic patients and it includes imaging and serological methods,although histopathological evaluation is still considered the gold standard diagnostic method.An effective established treatment is not yet available for patients with steatohepatitis and fibrosis and randomized clinical trials including only diabetic patients are lacking.We sought to outline the published data including epidemiology,pathogenesis,diagnosis and treatment of NAFLD in diabetic patients,in order to better understand the interplay between these two prevalent diseases and identify the gaps that still need to be fulfilled in the management of NAFLD in patients with diabetes mellitus. | Nathalie C Leite Cristiane A Villela-Nogueira Claudia R L Cardoso Gil F Salles | 2014 | World Journal of Gastroenterology2014，20，26： | 21 |
| 5 | Pre-diagnostic levels of adiponectin and soluble vascular cell adhesion molecule-1 are associated with colorectal cancer risk显示文摘AIM: To examine the relationships between pre-diagnostic biomarkers and colorectal cancer risk and assess their relevance in predictive models.METHODS: A nested case-control study was designed to include all first primary incident colorectal cancer cases diagnosed between inclusion in the SUpplémentation en VItamines et Minéraux AntioXydants cohort in 1994 and the end of follow-up in 2007. Cases (n = 50) were matched with two randomly selected controls (n = 100). Conditional logistic regression models were used to investigate the associations between pre-diagnostic levels of hs-CRP, adiponectin, leptin, soluble vascular cell adhesion molecule-1 (sVCAM-1), soluble intercellular adhesion molecule-1, E-selectin, monocyte chemoattractant protein-1 and colorectal cancer risk. Area under the receiver operating curves (AUC) and relative integrated discrimination improvement (RIDI) statistics were used to assess the discriminatory potential of the models. RESULTS: Plasma adiponectin level was associated with decreased colorectal cancer risk (P for linear trend = 0.03). Quartiles of sVCAM-1 were associated with increased colorectal cancer risk (P for linear trend = 0.02). No association was observed with any of the other biomarkers. Compared to standard models with known risk factors, those including both adiponectin and sVCAM-1 had substantially improved performance for colorectal cancer risk prediction (P for AUC improvement = 0.01, RIDI = 26.5%). CONCLUSION: These results suggest that pre-diagnostic plasma adiponectin and sVCAM-1 levels are associated with decreased and increased colorectal cancer risk, respectively. These relationships must be confirmed in large validation studies. | Mathilde Touvier Léopold Fezeu Namanjeet Ahluwalia Chantal Julia Nathalie Charnaux Angela Sutton Caroline Méjean Paule Latino-Martel Serge Hercberg Pilar Galan Sébastien Czernichow | 2012 | World Journal of Gastroenterology2012，18，22： | 15 |
| 6 | Anticancer immunotherapy by CTLA-4 blockade: obligatory contribution of IL-2 receptors and negative prognostic impact of soluble CD25显示文摘堵住抗体 ipilimumab 的细胞毒素的 T 淋巴细胞 antigen-4 (CTLA-4 ) 在很少的病人导致变形黑瘤的调停免疫者的长期的控制。尽管 ipilimumab 无疑经由 immunostimulation 施加它的治疗学的效果,这样远的临床上有用的、 immunologically 相关的 biomarkers 预言治疗效率是逃犯的。这里,我们显示出 IL-2 的那中立化或堵住 α并且 βIL-2 受体的子单元(CD25 和 CD122,分别地) 否则在现出症状之前的潜的老鼠模型由 CTLA-4 封锁导致了,废除了 antitumor 效果和 intratumoral T 受动器对规章的房间(Tregs ) 的比率的伴随的改进,它是。CTLA-4 封锁导致了在失去了 FoxP3 表示并且在 regressing 肿瘤积累了的 IL-2-producing 受动器房间与伴随物上升表示 Lag3, ICOS, IL-10 和 Egr2 的一个镇压 CD4 + T 房间子集的减小。当 recombinant IL-2 改进了 CTLA-4 封锁的治疗学的功效时,圈套 IL-2 受体 α(IL-2Rα, sCD25 ) 禁止了 CTLA-4 的 anticancer 效果封锁。在收到 ipilimumab 的 262 个变形黑瘤病人, sCD25 的基线浆液集中代表了全面幸存的独立指示物,与预言到治疗的抵抗的高水平。总的来说,这些结果解开为在 CTLA-4 的 anticancer 活动的 IL-2 和 IL-2 受体的一个角色封锁。重要地,我们的学习提供第一 immunologically 相关的 biomarker,也就是提高的浆液 sCD25,那与黑瘤在病人预言抵抗到 CTLA-4 封锁。 | Dalil Hannani Marie Vetizou David Enot Sylvie Rusakiewicz Nathalie Chaput David Klatzmann Melanie Desbois Nicolas Jacquelot Nadege Vimond Salem Chouaib Christine Mateus James P Allison Antoni Ribas Jedd D Wolchok Jianda Yuan Philip Wong Michael Postow Andrzej Mackiewicz Jacek Mackiewicz Dirk Schadendorff Dirk Jaeger Alan J Korman Keith Bahjat Michele Maio Luana Calabro Michele WL Teng Mark J Smyth Alexander Eggennont Caroline Robert Guido Kroemer Laurence Zitvogel | 2015 | Cell Research2015，25，2： | 14 |
| 7 | Albumin liver dialysis as pregnancy-saving procedure in cholestatic liver disease and intractable pruritus显示文摘Progressive familial intrahepatic cholestasis type 3 (PFIC3) is a rare cholestatic liver disease. Such liver disease can get worse by female hormone disorder. Albumin dialysis or Molecular Adsorbent Recirculating System (MARS) has been reported to reverse severe cholestasis-linked pruritus. Here, we report the first use of MARS during a spontaneous pregnancy and its successful outcome in a patient with PFIC3 and intractable pruritus. Albumin dialysis could be considered as a pregnancy-saving procedure in pregnant women with severe cholestasis and refractory pruritus. | Maud Lemoine Aurélie Revaux Claire Francoz Guillaume Ducarme Sabine Brechignac Emmanuel Jacquemin Michèle Uzan Nathalie Ganne-Carrié | 2008 | World Journal of Gastroenterology2008，14，42： | 14 |
| 8 | 一种求解带有阻塞限制的混合流水车间的禁忌搜索算法显示文摘针对带有阻塞限制的混合流水车间调度问题,提出一种禁忌搜索算法和优先级规则相结合的方法.开发了启发式调度算法求出问题初始解,应用禁忌搜索算法对工件在第一级的排序进行优化,采用优先级规则进行其他级工件的排序.实验结果表明,该方法大大缩减了搜索空间,能够较好地解决此类调度问题. | 陈璐 奚立峰 蔡建国 Nathalie Bostel Pierre Dejax | 2006 | 上海交通大学学报2006，40，5： | 11 |
| 9 | Schistosoma mansoni proteins attenuate gastrointestinal motility disturbances during experimental colitis in mice显示文摘AIM:To investigate the therapeutic effect of Schistosoma mansoni(S.mansoni) soluble worm proteins on gastrointestinal motility disturbances during experimental colitis in mice. METHODS:Colitis was induced by intrarectal injection of trinitrobenzene sulphate(TNBS) and 6 h later,mice were treated ip with S.mansoni proteins.Experiments were performed 5 d after TNBS injection.Inflammationwas quantified using validated inflammation parameters. Gastric emptying and geometric center were measured to assess in vivo gastrointestinal motility.Peristaltic activity of distal colonic segments was studied in vitro using a modified Trendelenburg set-up.Cytokine profiles of T-lymphocytes isolated from the colon were determined by real time reverse transcriptase-polymerase chain reaction. RESULTS:Intracolonic injection of TNBS caused severe colitis.Treatment with S.mansoni proteins significantly ameliorated colonic inflammation after 5 d.TNBS did not affect gastric emptying but significantly decreased the geometric center and impaired colonic peristaltic activity 5 d after the induction of colitis.Treatment with S.mansoni proteins ameliorated these in vivo and in vitro motility disturbances.In addition,TNBS injection caused a downregulation of effector T cell cytokines after 5 d,whereas a S.mansoni protein effect was no longer observed at this time point. CONCLUSION:Treatment with S.mansoni proteins attenuated intestinal inflammation and ameliorated motility disturbances during murine experimental colitis. | Nathalie E Ruyssers Benedicte Y De Winter Joris G De Man Natacha D Ruyssers Ann J Van Gils Alex Loukas Mark S Pearson Joel V Weinstock Paul A Pelckmans Tom G Moreels | 2010 | World Journal of Gastroenterology2010，16，6： | 11 |
| 10 | Partial Beclin 1 silencing aggravates doxorubicin-and Fasinduced apoptosis in HepG2 cells显示文摘瞄准:在 HepG2 房间的危险性上调查 Beclin 1 的角色在反船边交货抗体或 doxorubicin 治疗以后经历 apoptosis。方法:Beclin 1 silencing 用 RNA 干扰被完成。DNA 倍性, apoptotic 房间的百分比和 mitochondrial 膜电位被流动血细胞计数估计。多形核白细胞(自动数据处理核糖) 的 Beclin 1, Bcl-X (L) 和细胞色素 c,和劈开聚合酶(PARP ) 的层次是由使用西方的污点的 assayed。结果:Beclin 1 表示在 Beclin 1 siRNA transfection 以后由 75% 72 h 减少了。部分 Beclin 1 silencing 显著地增加了 subG1 房间的百分比有 doxorubicin 或反船边交货抗体的 24 和 40 h 术后疗法,分别地并且这 potentiation 被处理与一个 pan-caspase 禁止者废除。部分 Beclin 1 silencing 也增加了 PARP 劈开, mitochondrial 膜去极和 cytosolic 细胞色素 c。部分 Beclin 1 silencing 的 pro-apoptotic 后果没在 Bcl-X (L) 表示与衰落被联系。结论:部分 Beclin 1 silencing 在与反船边交货抗体或与 doxorubicin 对待的 HepG2 房间加重 mitochondrial permeabilization 和 apoptosis。 | Fanny Daniel Agnès Legrand Dominique Pessayre Nathalie Vadrot Véronique Descatoire Dominique Bernuau | 2006 | World Journal of Gastroenterology2006，12，18： | 11 |
| 11 | Signaling in Early Maize Kernel Development显示文摘 | Nicolas M. Doll Nathalie Depege-Fargei Peter M. Rogowsky Thomas Widiez | 2017 | Molecular Plant2017，10，3： | 9 |
| 12 | ABA-Mediated Inhibition of Germination Is Related to the Inhibition of Genes Encoding Cell-Wall Biosynthetic and Architecture: Modifying Enzymes and Structural Proteins in Medicago truncatula Embryo Axis显示文摘胚根出现和储备动员是被认为控制萌芽的二个不同计划。两个节目被 abscissic 酸(骆驼毛的织物) 影响,但是这荷尔蒙怎么控制种子萌芽,仍然糟糕被知道。Phenotypic 和 Medicago truncatula 的胚胎轴的显微镜的观察在 10 在处于有丝分裂的抑制状况的萌芽期间被触发 ? M oryzalin 证明房间分割没被要求允许胚根出现。镇压减少性的杂交证明在胚胎轴的超过 10% 起来调整的基因编码了与房间墙生合成有关的蛋白质。-expansins, pectin-esterase, xylogucan-endotransglycosidase,纤维素 synthase,和 extensins 的表示在种子的胚胎轴被监视在水上发芽了,经常、临时的骆驼毛的织物。在在控制并且强烈的萌芽的结束由骆驼毛的织物禁止了以前,这些基因是 overexpressed。在种子回来在水上被转移并且继续了到萌芽以后,表示在临时处理的 ABA 被重建。这作为贡献者证明这些基因到萌芽的结束并且加强在胚胎轴在与房间扩大的关系松开并且改变的房间墙是在萌芽的一个决定因素特征的想法。我们的结果也证明那件骆驼毛的织物通过胚根出现的控制控制萌芽,也就是由禁止房间墙松开和扩大。 | Christine Gimeno-Gilles Eric Lelievre Laure Viau Mustafa Malik-Ghulam Claudie Ricoult Andreas Niebel Nathalie Leduc Anis M. Limami | 2009 | Molecular Plant2009，2，1： | 8 |
| 13 | LncRNA-encoded peptides: More than translational noise?显示文摘 | Nathalie Rion Markus A R #x000FC egg | 2017 | Cell Research2017，27，5： | 8 |
| 14 | High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging显示文摘Progress in neuroscience relies on new techniques for investigating the complex dynamics of neuronal networks.An ongoing challenge is to achieve minimally invasive and high-resolution observations of neuronal activity in vivo inside deep brain areas.Recently introduced methods for holographic control of light propagation in complex media enable the use of a hair-thin multimode optical fibre as an ultranarrow imaging tool.Compared to endoscopes based on graded-index lenses or fibre bundles,this new approach offers a footprint reduction exceeding an order of magnitude,combined with a significant enhancement in resolution.We designed a compact and high-speed system for fluorescent imaging at the tip of a fibre,achieving a resolution of 1.18±0.04μm across a 50-μm field of view,yielding 7-kilopixel images at a rate of 3.5 frames/s.Furthermore,we demonstrate in vivo observations of cell bodies and processes of inhibitory neurons within deep layers of the visual cortex and hippocampus of anaesthetised mice.This study paves the way for modern microscopy to be applied deep inside tissues of living animal models while exerting a minimal impact on their structural and functional properties. | Sergey Turtaev Ivo T.Leite Tristan Altwegg-Boussac Janelle M.P.Pakan Nathalie L.Rochefort Tomáš Čižmár | 2018 | Light(Science & Applications)2018，7，1： | 8 |
| 15 | Caspase-1 activation by NLRP3 inflammasome dampens IL-33-dependent house dust mite-induced allergic lung inflammation显示文摘The cysteine protease caspase-1(Casp-1)contributes to innate immunity through the assembly of NLRP3,NLRC4,AIM2,and NLRP6 inflammasomes.Here we ask whether caspase-1 activation plays a regulatory role in house dust mite(HDM)-induced experimental allergic airway inflammation.We report enhanced airway inflammation in caspase-1-deficient mice exposed toHDMwith a marked eosinophil recruitment,increased expression of IL-4,IL-5,IL-13,aswell as full-length and bioactive IL-33.Furthermore,mice deficient for NLRP3 failed to control eosinophil influx in the airways and displayed augmented Th2 cytokine and chemokine levels,suggesting that the NLPR3 inflammasome complex controls HDM-induced inflammation.IL-33 neutralization by administration of soluble ST2 receptor inhibited the enhanced allergic inflammation,while administration of recombinant IL-33 during challenge phase enhanced allergic inflammation in caspase-1-deficient mice.Therefore,we show that caspase-1,NLRP3,and ASC,but not NLRC4,contribute to the upregulation of allergic lung inflammation.Moreover,we cannot exclude an effect of caspase-11,because caspase-1-deficient mice are deficient for both caspases.Mechanistically,absence of caspase-1 is associated with increased expression of IL-33,uric acid,and spleen tyrosine kinase(Syk)production.This study highlights acritical role of caspase-1 activation andNLPR3/ASCinflammasomecomplex in the down-modulation of IL-33 in vivo and in vitro,thereby regulating Th2 response in HDM-induced allergic lung inflammation. | Fahima Madouri Noelline Guillou Louis Fauconnier Tiffany Marchiol Nathalie Rouxel Pauline Chenuet Aurelie Ledru Lionel Apetoh Franc¸ois Ghiringhelli Mathias Chamaillard Song Guo Zheng Fabrice Trovero Valerie F.J.Quesniaux Bernhard Ryffel Dieudonnee Togbe | 2015 | Journal of Molecular Cell Biology2015，7，4： | 8 |
| 16 | 用基因组原位杂交方法分析甘蔗-斑茅杂种及回交后代的染色体组成显示文摘斑茅(Erianthus arundinaceus Retz)是甘蔗的近缘属植物,具有多种优良特性,是甘蔗育种的重要种质资源之一。本实验使用基因组原位杂交(GISH)技术分析了甘蔗-斑茅杂种及回交后代F1、BC1和BC2的染色体构成与传递行为。结果表明:在F1代,来自斑茅HN92-77的染色体数目介于28~30条,来自热带种Badila的染色体数目介于38~40条,体细胞染色体数为2n=68~70,基本符合n+n的染色体传递方式;在BC1和BC2代,来自斑茅的染色体数分别为22~28条和13~15条,来自甘蔗的染色体数分别是87~94条和98~101条,其体细胞染色体数2n分别为110~121条和112~115条,基本上分别符合2n+n和n+n的染色体传递方式。在所观察的渐渗系中,均存在有染色体丢失的现象,但未观察到染色体发生交换与重组。 | 陈健文 Nathalie Piperidis (nee Reffay) 李奇伟 陈勇生 符成 Phillip Jackson George Pipendis 邓海华 | 2010 | 分子植物育种2010，8，2： | 8 |
| 17 | Role of Arabidopsis UV RESISTANCE LOCUS 8 in Plant Growth Reduction under Osmotic Stress and Low Levels of UV-B显示文摘在热点环境,植物暴露于压力的不同类型,例如触发普通 morphogenic 的 UV-B,而且 droughtstress 的过量适应反应导致植物生长的一般减小。这里,我们 reportthat Arabidopsis thaliana 紫外抵抗地点 8 (UVR8 ) 基因, UV-B morphogenic 反应的一个已知的管理者,能补充 Saccharomyces cerevisiae osmo 敏感的异种和它的表示在 Arabidopsis 植物在渗透或咸的应力以后被导致。在 UV-B 的底层下面,植物 overexpressing UVR8 与减少的根开发被相形见绌并且与控制植物相比积累更多的 flavonoids。生长 defectsare 主要由于细胞扩大的抑制。UVR8 overexpression 在 UV-B 的底层下面在植物触发的生长抑制被导致甘露糖醇的渗透的应力加重,但是它不是由离子的应力的 significantlyaffected。相反, uvr8-6 异种植物不在标准条件下面不同于野类型的植物,但是他们显示出一个增加的射击生长 underhigh 盐压力。我们的数据建议 flavonoid 并且可能的调停 UVR8 的累积在植物生长素 homeostasisare 改变观察生长显型的内在的机制并且 UVR8 可能为 integratingplant 生长和压力 signals.Key 词有一个重要角色: | Rossella Fasano Nathalie Gonzalez Alessandra Tosco Fabrizio Dal Piaz Teresa Docimo Ramon Serrano Stefania Grillo Antonella Leone Dirk Inze | 2014 | Molecular Plant2014，7，5： | 7 |
| 18 | Dysferlin缺陷:肢带2B型肌营养不良与Miyoshi肌病的致病原因显示文摘目的 对临床怀疑为常染色体隐性遗传性肌营养不良一家系进行分析 ,以明确肌病类型并寻找其致病基因的分子缺陷。方法 用与 8种常染色体隐性遗传性肌营养不良基因连锁的短串联重复序列标记进行连锁分析 ,用与 5种肌营养不良相关的单克隆抗体作多重免疫印迹分析检测相应致病基因的编码产物 ;通过逆转录 - PCR扩增先证者致病基因的编码序列并测序 ,确定基因突变。结果 家系连锁分析显示在 DYSF基因附近的 D2 S337位点的优势对数记分值为 1.85 ,提示致病基因与 D2 S337连锁 ;免疫印迹分析提示患者DYSF基因的编码产物 dysferlin缺陷 ;测序证明先证者DYSF基因的c DNA第 6 4 2 9位发生纯合性del G突变。结论 综合研究结果和临床资料 ,这一家系中的先证者被诊断为 Miyoshi肌病 ,由DYSF基因纯合性缺失突变所导致。 | 孙顺昌 樊绮诗 吴华成 France Leturcq 张炳峰 于文 Nathalie Deburgrave 刘明 宋永建 | 2004 | 中华医学遗传学杂志2004，21，2： | 7 |
| 19 | 应用ELISPOT试验测定重组卡介苗诱导的T细胞应答显示文摘应用免疫磁性分离技术去除免疫小鼠脾脏细胞中CD4+或CD8+T细胞后 ,在酶联免疫斑点试验中证实表达大肠杆菌MalE蛋白的重组卡介苗 (rBCG·MalE)诱导的T细胞应答是CD4+T细胞依赖的。对MalE、PPD特异T细胞应答的动态分析结果表明 ,rBCG·MalE、BCG诱导的特异CD4+T细胞应答存在Th1/Th2平衡转换现象 ,即起始阶段为Th1应答 ,一段时间后出现Th2应答 ,并逐步形成Th1/Th2混合应答。这些结果 ,为分枝杆菌T细胞应答规律提供了新的认识 ,同时 ,亦表明BCG是优良的外源抗原表达与运送载体。 | 焦新安 RichardLo-man EdithD’eriaud Brigitte Gicquel Nathalie Winter Claude Leclerc 刘秀梵 | 2001 | 中国预防兽医学报2001，23，1： | 7 |
| 20 | 集装箱码头装卸作业的调度控制模型及算法设计显示文摘对集装箱码头上装卸作业的调度控制直接影响码头的整体运营效率.本文研究了集装箱码头装卸作业的调度控制问题,提出了一个基于柔性化flow shop的集成化控制模型.该问题具有非线性规划(NP:non- polynomial)难度,因此本文开发设计了两类基于优先级规则的启发式调度算法.利用该模型来对码头中多种装卸设备进行总体调度可以提高设备之间的协调性,提高码头效率,降低成本.实验研究证明算法能有效地解决该问题. | 陈璐 奚立峰 蔡建国 Nathalie BOSTEL Pierre DEJAX | 2006 | 控制理论与应用2006，23，6： | 6 |
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