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| 1 | Promoter hypermethylation of CDH1, FHIT, MTAP and PLAGL1 in gastric adenocarcinoma in individuals from Northern Brazil显示文摘AIM: To evaluate the methylation status of CDH1, FHIT, MTAP and PLAGL1 promoters and the association of these findings with clinico-pathological characteristics. METHODS: Methylation-specific PCR (MSP) assay was performed in 13 nonneoplastic gastric adenocarcinoma, 30 intestinal-type gastric adenocarcinoma and 35 diffuse- type gastric adenocarcinoma samples from individuals in Northern Brazil. Statistical analyses were performed using the chi-square or Fisher’s exact test to assess associations between methylation status and clinico- pathological characteristics. RESULTS: Hypermethylation frequencies of CDH1, FHIT, MTAP and PLAGL1 promoter were 98.7%, 53.9%, 23.1% and 29.5%, respectively. Hypermethylation of three or four genes revealed a significant association with diffuse-type gastric cancer compared with nonneoplastic cancer. A higher hypermethylation frequency wassignificantly associated with H pylori infection in gastric cancers, especially with diffuse-type. Cancer samples without lymph node metastasis showed a higher FHIT hypermethylation frequency. MTAP hypermethylation was associated with H pylori in gastric cancer samples, as well as with diffuse-type compared with intestinal-type. In diffuse-type, MTAP hypermethylation was associated with female gender. CONCLUSION: Our findings show differential gene methylation in tumoral tissue, which allows us to conclude that hypermethylation is associated with gastric carcinogenesis. MTAP promoter hypermethylation can be characterized as a marker of diffuse-type gastric cancer, especially in women and may help in diagnosis, prognosis and therapies. The H pylori infectious agent was present in 44.9% of the samples. This infection may be correlated with the carcinogenic process through the gene promoter hypermethylation, especially the MTAP promoter in diffuse-type. A higher H pylori infection in diffuse-type may be due to greater genetic predisposition. | Mariana Ferreira Leal Eleonidas Moura Lima Patrícia Natália Oliveira Silva Paulo Pimentel Assumpo Danielle Queiroz Calcagno Spencer Luiz Marques Payo Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith | 2007 | World Journal of Gastroenterology2007，13，18： | 24 |
| 2 | Renin angiotensin system in liver diseases: Friend or foe?显示文摘In the last three decades,the understanding of the renin angiotensin system(RAS)has been changed by the discoveries of functional local systems,novel biologically active peptides,additional specific receptors,alternative pathways of angiotensin(Ang)?Ⅱ?generation,and new roles for enzymes and precursor components other than those in Ang?Ⅱ?synthesis.In this regard,the discovery that Ang-(1-7)opposes the pressor,proliferative,pro-fibrotic,and pro-inflammatory effects mediated by Ang?Ⅱ?has contributed to the realization that the RAS is composed of two axes.The first axis consists of the angiotensin-converting enzyme(ACE),with Ang?Ⅱ?as the end product,and the angiotensin type 1(AT1)receptor as the main effector mediating the biological actions of Ang?Ⅱ.The second axis results from ACE2-mediated hydrolysis of Ang?Ⅱ,leading to the production of Ang-(1-7),with the Mas receptor as the main effector conveying the vasodilatory,antiproliferative,anti-fibrotic,and anti-inflammatory effects of Ang-(1-7).Experimental and clinical studies have shown that both axes of the RAS may take part in the pathogenesis of liver diseases.In this manuscript,we summarize the current evidence regarding the role of RAS in hepatic cirrhosis and its complications,including hemodynamic changes and hepatorenal syndrome.The therapeutic potential of the modulation of RAS molecules in liver diseases is also discussed. | Ana Cristina Simoes e Silva Aline S Miranda Natália P Rocha Antonio L Teixeira | 2017 | World Journal of Gastroenterology2017，23，19： | 12 |
| 3 | Relevance of MUC1 mucin variable number of tandem repeats polymorphism in H pylori adhesion to gastric epithelial cells显示文摘AIM:To evaluate the influence of MUC1 mucin variable number of tandem repeats (VNTR) variability on H pylori adhesion to gastric cells. METHODS: Enzyme linked immunosorbent assay (ELISA)-based adhesion assays were performed to measure the adhesion of different H pylori strains (HP26695 and HPTx30a) to gastric carcinoma cell lines (GP202 and MKN45) and GP202 clones expressing recombinant MUC1 with different VNTR lengths. RESULTS: Evaluation of adhesion results shows that H pylori pathogenic strain HP26695 has a significantly higher (P < 0.05) adhesion to all the cell lines and clones tested, when compared to the non-pathogenic strain HPTx30a. Bacteria showed a significantly higher (P < 0.05) adhesion to the GP202 cell line, when compared to the MKN45 cell line. Furthermore, both strains showed a significantly higher (P < 0.05) adhesion to GP202 clones with larger MUC1 VNTR domains. CONCLUSION: This work shows that MUC1 mucin variability conditions H pylori binding to gastric cells. The extent of bacterial adhesion depends on the size of theMUC1 VNTR domain. The adhesion is further dependent on bacterial pathogenicity and the gastric cell line. MUC1 mucin variability may contribute to determine H pylori colonization of the gastric mucosa. | Natália R Costa Nuno Mendes Nuno T Marcos Celso A Reis Thomas Caffrey Michael A Hollingsworth Filipe Santos-Silva | 2008 | World Journal of Gastroenterology2008，14，9： | 4 |
| 4 | 日本进行HCV、HBV和HIV病毒核酸筛查的现状显示文摘日本红十字会输血部对自愿献血者血液的细胞成份以及做血浆蛋白分离的血浆,在预筛后与发出前,第一次在全国范围用核酸扩增检测(NAT)方法检测血液中的乙型肝炎病毒(HBV)、丙型肝炎病毒(HCV)和人类免疫缺陷病毒(HIV-1)。使用多重NAT试剂对血液和血液制品,包括存活时间短的血小板检测,可节省检测时间、费用和人力。在2000年2月1日-2001年4月30日期间,采用50份血样品混合筛查,从血液和血液成分中查出112份HBV阳性、25份HCV阳性和4份HIV-1阳性。 | T.Tomono和日本红十字会NAT筛查研究组 李执如 周琼秀 | 2003 | 国外医学(输血及血液学分册)2003，26，2： | 4 |
| 5 | Intravenous vs intraperitoneal mesenchymal stem cells administration:What is the best route for treating experimental colitis?显示文摘AIM:To investigate the therapeutic effects of mesenchymal stem cells(MSCs)transplanted intraperitoneally andintravenously in a murine model of colitis.METHODS:MSCs were isolated from C57BL/6 mouse adipose tissue.MSC cultures were analyzed according to morphology,cellular differentiation potential,and surface molecular markers.Experimental acute colitis was induced in C57BL/6 mice by oral administration of2%dextran sulfate sodium(DSS)in drinking water ad libitum from days 0 to 7.Colitis mice were treated with1×106 MSCs via intraperitoneal or intravenous injection on days 2 and 5.The disease activity index was determined daily based on the following parameters:weight loss,stool consistency and presence of blood in the feces and anus.To compare morphological and functional differences in tissue regeneration between different MSC injection modalities,mice were euthanized on day 8,and their colons were examined for length,weight,and histopathological changes.Inflammatory responses were determined by measuring the levels of different serum cytokines using a CBA Th1/Th2/Th17 kit.Apoptotic rates were evaluated by terminal deoxynucleotidyl transferase-mediated d UDPbiotin nick end labeling assay.RESULTS:Intravenous infusion of MSCs was more effective than intraperitoneal treatment(P<0.001)in reducing the clinical and histopathologic severity of colitis,which includes weight loss,diarrhea and inflammation.An histological evaluation demonstrated decreased colonic inflammation based on reduced crypt loss and reduced infiltration of inflammatory cells.This therapeutic effect was most likely mediated by the down-regulation of pro-inflammatory cytokines[interleukin(IL)-6 and tumor necrosis factor(TNF)];and by the up-regulation of anti-inflammatory cytokines(IL-10 and IL-4).Intravenous transplantation alsoinduced high levels of IFN that lead to activation of the immunosuppressive activity of the MSCs,which did not occur with intraperitoneal transplantation(P=0.006).An increase in apoptotic T cells was observed after intravenous,but not intraperitoneal,MSC infusion,suggesting that MSCs can induce apoptosis in resistant T cells in colonic inflammation(P=0.027).CONCLUSION:Our results demonstrate that intravenous treatment is a superior method for reducing colon inflammation compared with intraperitoneal therapy. | Fabiany da Costa Goncalves Natália Schneider Fernanda Otesbelgue Pinto Fabíola Schons Meyer Fernanda Visioli Bianca Pfaffenseller Patrícia Luciana da Costa Lopez Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima Luíse Meurer Ana Helena Paz | 2014 | World Journal of Gastroenterology2014，20，48： | 4 |
| 6 | Screening for Spikelet Fertility and Validation of Heat Tolerance in a Large Rice Mutant Population显示文摘A total of 10 000 M4 individuals in Jao Hom Nil (JHN) mutant population was treated with high temperature (40 ℃ to 45 ℃) during the day time (6 h) from the booting to the harvesting stages, and ambient temperature (33 ℃ to 35℃) was used as the control. The results of screening and yield trials found that the mutant line M9962 had a high spikelet fertility of 78% under heat stress. In addition, the other mutant lines, including M3181 and M7988, had a spikelet fertility of approximately 70%. However, the JHN wild type, Sin Lek, RD15 and RD33 had very low spikelet fertility of 34%, 14%, 9% and 4%, respectively. The lower spikelet fertility at an elevated temperature resulted in a dramatic decrease of filled grain and contributed to a loss in 100-grain weight. M9962 is a potential genetic stock for use in a heat tolerance breeding programme. In addition, spikelet fertility at high temperature was representative of heat tolerance and can be used as a screening trait for heat tolerance during the reproductive phase on a large scale. | Sulaiman CHEABU Nat PANICHAWONG Prisana RATTANAMETTA Boonthong WASURI Poonpipope KASEMSAP Siwaret ARIKIT Apichart VANAVICHIT Chanate MALUMPONG | 2019 | Rice science2019，26，4： | 3 |
| 7 | Spatial features of land use/land cover change in the United States显示文摘With the classifi cation data covering American land-use/land-cover (LUCC) with 30 m resolu tion from the project of National Land Cover Data (NLCD), we normalize d them and made their resolution changed into 1 km ×1 km, created the data of American land-use grade and analyzed the spatial distribution and featur es of American LUCC as well as the influence of population and altit ude on the land-use grade in light of methods of sampling analysis a nd correlation study. Based on the analysis, we concluded that forestr y and grassland, accounting for 71.24% of the whole country, has taken the main part of American land cover, and besides, construction and arable land has occupied 19.22% of the total land, the rest of land cover types, including water area, wetland and underdeveloped land, is 9. 54% of the country’s total. The developing potential of American land resources is enormous with less destroyed and disturbed ecological environment. Although, in some sense, the population and altitude influence the sp atial variation of American land-use grade respectively, the influence of spatial variation of altitude and population density on that of la nd-use grade is not significanct. | GAO Zhiqiang,LIU Jiyuan,DENG Xiangzheng(Inst. of Geographic Sciences and Nat ural Resources Research, CAS, Beijing 100101, China) | 2003 | Journal of Geographical Sciences2003，13，1： | 3 |
| 8 | Resveratrol attenuates oxidative stress and prevents steatosis and hypertension in obese rats programmed by early weaning显示文摘 | Juliana G. Franco Patrícia C. Lisboa Natália S. Lima Taline A.S. Amaral Nayara Peixoto-Silva Angela C. Resende Elaine Oliveira Magna C.F. Passos Egberto G. Moura | 2013 | The Journal of Nutritional Biochemistry2013，，6： | 2 |
| 9 | How to manage inflammatory bowel disease during the COVID-19 pandemic:A guide for the practicing clinician显示文摘Managing inflammatory bowel disease(IBD)during the coronavirus disease 2019(COVID-19)pandemic has been a challenge faced by clinicians and their patients,especially concerning whether to proceed with biologics and immunosuppressive agents in the background of a global outbreak of a highly contagious new coronavirus(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2).The knowledge about the impact of this virus on patients with IBD,although it is still scarce,is rapidly evolving.In particular,concerns surrounding medications’impact for IBD on the risk of acquiring SARS-CoV-2 infection or developing COVID-19,and potentially exacerbate viral replication and the COVID-19 course,are a current thinking of both practicing clinicians and providers caring for patients with IBD.Managing patients with IBD infected with SARS-CoV-2 depends on both the clinical activity of the IBD and the occasional development and severity of COVID-19.In this review,we summarize the current data regarding gastrointestinal involvement by SARS-CoV-2 and pharmacologic and surgical management for IBD concerning this infection,and the COVID-19 impact on both the patient's psychological functioning and endoscopy services,and we concisely summarize the telemedicine roles during the COVID-19 pandemic. | Júlio Maria Fonseca Chebli Natália Sousa Freitas Queiroz Adérson Omar Mourão Cintra Damião Liliana Andrade Chebli Márcia Henriques de Magalhães Costa Rogério Serafim Parra | 2021 | World Journal of Gastroenterology2021，27，11： | 2 |
| 10 | 氮素改变辣椒初级代谢和薄壁组织厚度调控比叶面积研究显示文摘比叶面积(SLA)是影响植物光截获和光利用效率的关键性状,常常影响植物的生长和产量。SLA是解释不同植物在不同环境下生长差异的一个关键性状。光照和氮素供应都是决定SLA的重要因素。为了更好地了解辐照度和氮素对辣椒(Capsicum chinense)SLA的影响,研究对两个中国辣椒商品种(Biquinho和Habanero)的初级代谢产物和形态特征进行了分析。两种基因型辣椒的SLA随着遮阴而增加,但随氮供应量的增加而下降;在氮素供应由缺乏到充足的范围内Habanero品种的SLA表现稳定。相关分析表明,高氮供应导致SLA降低是因为氨基酸、蛋白质和淀粉含量的调节,从而影响叶片密度。此外,在中度缺氮到充足供氮范围内,两种基因型辣椒的SLA表现出响应差异,Biquinho和Habanero分别在栅栏和海绵状薄壁组织上表现出差异。总之,结果表明,SLA对氮供应的反应是由某些代谢物含量和依赖于基因型的影响叶片厚度及密度的薄壁组织变化平衡调节的。 | Lucas de ávila Silva Rebeca P.Omena-Garcia Jorge A.Condori-Apfata Paulo Mafra de Almeida Costa Natália Machado Silva Fábio M.Da Matta Agustin Zsogon Wagner L.Araújo Edgard A.de Toledo Picoli Ronan Sulpice Adriano Nunes-Nesi 沈凌峰 吉雪花(译) | 2021 | 辣椒杂志2021，，3： | 2 |
| 11 | OVERVIEW OF COMPLEX SYSTEMS IN SPORT显示文摘The complex systems approach offers an opportunity to replace the extant pre-dominant mechanistic view on sport-related phenomena.The emphasis on the environment-system relationship,the applications of complexity principles,and the use of nonlinear dynamics mathematical tools propose a deep change in sport science.Coordination dynamics,ecological dynamics,and network approaches have been successfully applied to the study of different sport-related behaviors,from movement patterns that emerge at different scales constrained by specific sport contexts to game dynamics.Sport benefit from the use of such approaches in the understanding of technical,tactical,or physical conditioning aspects which change their meaning and dilute their frontiers.The creation of new learning and training strategies for teams and individual athletes is a main practical consequence.Some challenges for the future are investigating the influence of key control parameters in the nonlinear behavior of athlete-environment systems and the possible relatedness of the dynamics and constraints acting at different spatio-temporal scales in team sports.Modelling sport-related phenomena can make useful contributions to a better understanding of complex systems and vice-versa. | BALAGUE Natàlia TORRENTS Carlota HRISTOVSKI Robert DAVIDS Keith ARAUJO Duarte | 2013 | Journal of Systems Science & Complexity2013，26，1： | 2 |
| 12 | Non-pharmacological management of pediatric functional abdominal pain disorders:Current evidence and future perspectives显示文摘Functional abdominal pain disorders(FAPDs) are an important and prevalent cause of functional gastrointestinal disorders among children, encompassing the diagnoses of functional dyspepsia, irritable bowel syndrome, abdominal migraine, and the one not previously present in Rome Ⅲ, functional abdominal pain not otherwise specified. In the absence of sufficiently effective and safe pharmacological treatments for this public problem, non-pharmacological therapies emerge as a viable means of treating these patients, avoiding not only possible side effects, but also unnecessary prescription, since many of the pharmacological treatments prescribed do not have good efficacy when compared to placebo. Thus, the present study provides a review of current and relevant evidence on non-pharmacological management of FAPDs, covering the most commonly indicated treatments, from cognitive behavioral therapy to meditation, acupuncture, yoga, massage, spinal manipulation, moxibustion, and physical activities. In addition, this article also analyzes the quality of publications in the area, assessing whether it is possible to state if non-pharmacological therapies are viable, safe, and sufficiently well-based for an appropriate and effective prescription of these treatments. Finally, it is possible to observe an increase not only in the number of publications on the non-pharmacological treatments for FAPDs in recent years, but also an increase in the quality of these publications. Finally, the sample selection of satisfactory age groups in these studies enables the formulation of specific guidelines for this age group, thus avoiding the need for adaptation of prescriptions initially made for adults, but for children use. | Maria Luísa Cordeiro Santos Ronaldo Teixeira da Silva Júnior Breno Bittencourt de Brito Filipe Antônio França da Silva Hanna Santos Marques Vinícius Lima de SouzaGonçalves Talita Costa dos Santos Carolina Ladeia Cirne Natália Oliveira e Silva Márcio Vasconcelos Oliveira Fabrício Freire de Melo | 2022 | World Journal of Clinical Pediatrics2022，11，2： | 2 |
| 13 | Development of the asthma control test:a survey for assessing asthma control显示文摘 | Nat han RA Sorkness CA Kosinski M | 2004 | J Allergy Clin Immunol2004，113，1： | 1 |
| 14 | Methane emission from tid- al freshwater marshes显示文摘 | Van der Nat F J W A Middelburg J J | 2000 | Biogeochemistry2000，49，： | 1 |
| 15 | DETERMINATION OF CHLORFENVINPHOS, FIPRONIL, AND CYPERMETHRIN RESIDUES IN MEAT AND BOVINE FAT USING QUECHERS METHOD AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY显示文摘 | NatáliaCecília Sartarelli AdrianaNori de Macedo JulianaPane de Sousa AnaRita de Araujo Nogueira SilviaHelena Govoni Brondi | 2012 | Journal of Liquid Chromatography & Related Technologies2012，，13： | 1 |
| 16 | Diel methane emission patterns from Scirpus lacustris and Phragmites australis显示文摘 | Van der Nat FJWA Middelburg JJ Van Meteren D | 1998 | Biogeochemistry1998，41，1： | 1 |
| 17 | Status of NAT screening for HCV, HIV and HBV : experience in Japan显示文摘 | Japanese Red Cross NAT Screening Research Group | 2002 | Dev Biol (Basel)2002，108，： | 1 |
| 18 | Results of molecular detection of Mycoplasma pneumoniae among patients with acute respiratory infection and in their honsehold contacts reveals children as human reservoirs显示文摘 | Dorigo-Zatsma JW Wilbrink B van der Nat | 2001 | J Infect Dis2001，183，4： | 1 |
| 19 | Electroanatomic versus fluoroscopic mapping for catheter ablation procedures:A prospective randomized study显示文摘 | Sporton S Earley M Nat han A | 2004 | J Cardiovasc Electrophysiol2004，15，： | 1 |
| 20 | Strategies for management of prostate cancer related bone pain显示文摘 | Pelger RCM Soerdjbalie-Mailkoe V Hamdy NAT | 2001 | Drugs Aging2001，18，12： | 1 |
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