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| 1 | NS1 antigen: A new beam of light in the early diagnosis of dengue infection显示文摘Objective: To evaluate NS1 antigen detection ELISA for the early laboratory diagnosis of dengue virus infection. Methods: The present study was conducted to evaluate the overall positivity of NS1 antigen detection ELISA and its comparison with viral RNA detection via real time PCR and Ig M antibodies detection by ELISA. Results: A total of 1 270 serum samples were tested 86%(1 097/1 270) were detected positive by one or more than one diagnostic test. Out of 1 270, 64%(807/1 270) were positive by NS1 ELISA and 52%(662/1 270), 51%(646/1 270) were positive by real-time RT-PCR and Ig M ELISA respectively.Conclusions: NS1 antigen detection ELISA is highly suitable diagnostic tools and it also has great value for use in outbreak and epidemic situation. | Muhammad Suleman Rani Faryal Muhammad Masroor Alam Salmaan Sharif Shahzad Shaukat Uzma Bashir Aamir Adnan Khurshid Mehar Angez Massab Umair Mian Muhammad Sufian Yasir Arshad Ghulam Mujtaba Syed Sohail Zahoor Zaidi | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，12： | 6 |
| 2 | ‘Human bocavirus in Pakistani children with gastroenteritis’显示文摘 | Muhammad Masroor Alam Adnan Khurshid Shahzad Shaukat Salmaan Sharif Rana Muhammad Suleman Mehar Angez Nadia Nisar Uzma Bashir Aamir Muhammad Naeem Syed Sohail Zahoor Zaidi | 2015 | J. Med. Virol2015，，： | 1 |
| 3 | The outbreak of dengue during the COVID-19 pandemic in Pakistan:The emergence of overlapping crises显示文摘COVID-19 pandemic has become a serious public health crisis for developed and developing countries around the world.As of February 17,2022,over 420 million cases including 5.8 million deaths have been reported across the globe[1].The first case of COVID-19 in Pakistan was detected on February 26,2020;since then,the toll of confirmed COVID-19 cases reached 1491423 including 29877 deaths as of February 17,2022[1]. | Muhammad Suleman Rana Muhammad Usman Muhammad Masroor Alam Aamer Ikram Muhammad Salman Rani Faryal Massab Umair | 2022 | Asian Pacific Journal of Tropical Medicine2022，15，2： | 0 |
| 4 | Dengue outbreak in Swat and Mansehra,Pakistan 2013;an epidemiological and diagnostic perspective显示文摘Objective:To High light some epidemiological,clinical and diagnostic features of dengue fever during an outbreak and the role of different diagnostic techniques to achieve the highest level of accuracy in results.Methods:Blood samples(n=323) were collected along with epidemiological and clinical data from suspected dengue patients who visited different hospitals in Swat and Mansehra district of Pakistan between May-November 2013 during a dengue outbreak.Samples were tested for the detection of viral nucleic acid by real-lime PCR.non structural protein-1(NS1antigen and IgM antibodies by ELISA.Results:Out of 323 cases with clinical dengue infection,304 were positive by one or more diagnostic parameter:201 samples were positive by real-time PCR,209 were positive by NS1 ELISA and 190 were positive by IgM antibodies.Sensitivities of real-time PCR and NS1 F.LISA were comparable for early diagnosis of dengue virus infection.IgM antibody detection assay was found useful for the diagnosis in the samples collected later than day 5 of onset.Conclusions:The use of real-lime PCR or detection of non stnictural protein NS 1 by ELISA followed by IgM antibodies detection can be recommended for early diagnosis of dengue virus infection with a high level of accuracy. | Muhammad Suleman Rani Faryal Uzma Bashir Aamir Muhammad Masroor Alam Nadia Nisar Salmaan Sharif Shahzad Shaukat Adnan Khurshid Mehar Angez Massab Umair Ghulam Mujtaba Mian Muhammad Sufian Yasir Arshad Lubna Rehman Syed Sohail Zahoor Zaidi | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，4： | 0 |
| 5 | Preliminary Seroepidemiological survey of dengue infections in Pakistan,2009-2014显示文摘Background:Dengue virus is the causative agent of dengue fever,a vector borne infection which causes selflimiting to life threatening disease in humans.A sero-epidemiological study was conducted to understand the current epidemiology of dengue virus in Pakistan which is now known as a dengue endemic country after its first reported outbreak in 1994.Methods:To investigate the prevalence of dengue virus in Pakistan during 2009-2014,a total of 9,493 blood samples were screened for the detection of anti-dengue IgM antibodies using ELISA.Clinical and demographic features available with hospital records were reviewed to ascertain mortalities related to dengue hemorrhagic shock syndrome.Results:Out of 9,493 samples tested,37%(3,504)were found positive for anti-dengue IgM antibodies.Of the seropositive cases,73.6%(2,578/3,504)were male and 26.4%(926/3,504)were female.The highest number(382/929;41.1%)of sero-positive cases was observed among the individuals of age group 31-40 years.The highest number of symptomatic cases was reported in October(46%;4,400/9,493),and the highest number of sero-positive cases among symptomatic cases was observed in November(45.7%;806/1,764).Mean annual patient incidence(MAPI)during 2009-2014 in Pakistan remained 0.30 with the highest annual patient incidence(11.03)found in Islamabad.According to the available medical case record,472 dengue related deaths were reported during 2009-2014.Conclusion:The data from earlier reports in Pakistan described the dengue virus incidence from limited areas of the country.Our findings are important considering the testing of clinical samples at a larger scale covering patients of vast geographical regions and warrants timely implementation of dengue vector surveillance and control programs.Trial registration number:It is an epidemiological research study,so trial registration is not required. | Muhammad Suleman Hyeong-Woo Lee Syed Sohail Zahoor Zaidi Muhammad Masroor Alam Nadia Nisar Uzma Bashir Aamir Salmaan Sharif Shahzad Shaukat Adnan Khurshid Mehar Angez Massab Umair Ghulam Mujtaba Rani Faryal | 2017 | Infectious Diseases of Poverty2017，6，1： | 0 |
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