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| 1 | Preclinical evaluation of azathioprine plus buthionine sulfoximine in the treatment of human hepatocarcinoma and colon carcinoma显示文摘AIM: To evaluate the efficacy and the safety of azathioprine (AZA) and buthionine sulfoximine (BSO) bylocalized application into HepG2 tumor in vivo.METHODS: Different hepatoma and colon carcinoma cell lines (HepG2, HuH7, Chang liver, LoVo, RKO, SW-48, SW-480) were grown in minimal essencial medium supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained in a humidified 37 ℃ incubator with 5% CO2. These cells were pretreated with BSO for 24 h and then with AZA for different times. We examined the effects of this combination on some proteins and on cellular death. We also studied the eff icacy and the safety of AZA (6 mg/kg per day) and BSO (90 mg/kg per day) in HepG2 tumor growth in vivo using athymic mice. We measured safety by serological markers such as aminotransferases and creatine kinase.RESULTS: The in vitro studies revealed a new mechanism of action for the AZA plus BSO combination in the cancer cells compared with other thiopurines (6-mercaptopurine, 6-methylmercaptopurine, 6-thioguanine and 6-methylthioguanine) in combination with BSO. The cytotoxic effect of AZA plus BSO in HepG2 cells resulted from necroptosis induction in a mitochondrial-dependent manner. From kinetic studies we suggest that glutathione (GSH) depletion stimulates c-Jun amino-terminal kinase and Bax translocation in HepG2 cells with subsequent deregulation of mitochondria (cytochrome c release, loss of membrane potential), and proteolysis activation leading to loss of membrane integrity, release of lactate dehydrogenase and DNA degradation. Some of this biochemical and cellular changes could be reversed by N-acetylcysteine (a GSH replenisher). In vivo studies showed that HepG2 tumor growth was inhibited when AZA was combined with BSO.CONCLUSION: Our studies suggest that a combination of AZA plus BSO could be useful for localizedtreatment of hepatocellular carcinoma as in the currently used transarterial chemoembolization method. | Borja Hernández-Breijo Jorge Monserrat Sara Ramírez-Rubio Eva P Cuevas Diana Vara Inés Díaz-Laviada M Dolores Fernández-Moreno Irene D Román Javier P Gisbert Luis G Guijarro | 2011 | World Journal of Gastroenterology2011，17，34： | 2 |
| 2 | Validation and comparison of advanced differential interferometry techniques: murcia metropolitan area case study显示文摘 | HERRERA G TOMAS R LOPEZ-SANCHEZ J M DELGADO J VICENTE F MULAS J COOKSLEY G SANCHEZ M DURO J ARNAUD A BLANCO P DUQUE S MALLORQUI J J DE la VEGA-PANIZO R MONSERRAT O | 2008 | ISPRS Journal of Photogrammetry and Remote Sensing2008，，： | 1 |
| 3 | Toxicological effect of hepatotoxins (microcystin) on aquatic organisms显示文摘 | Monserrat J M Pinho G L L Yunes J S | 2003 | Comments on Toxicology2003，9，： | 1 |
| 4 | Sudden death in hypertrophic cardiomyopathy: identification of high risk patients显示文摘 | Perry M Elliott Jan Poloniecki Shaughan Dickie Sanjay Sharma Lorenzo Monserrat Amanda Varnava Niall G Mahon William J McKenna | 2000 | Journal of the American College of Cardiology2000，，7： | 1 |
| 5 | Sudden death in hypertrophic cardiomyopathy: identification of high risk patients显示文摘 | Perry M Elliott Jan Poloniecki Shaughan Dickie Sanjay Sharma Lorenzo Monserrat Amanda Varnava Niall G Mahon William J McKenna | 2000 | Journal of the American College of Cardiology2000，，7： | 1 |
| 6 | Artichoke (Cynara scolymus L) modifies bacterial enzymatic activities and antioxidant status in rat cecum显示文摘 | ISABEL G ANTONIO JE MONSERRAT G | 2005 | Nutrition Research2005，25，6： | 1 |
| 7 | A review of ground-based SAR interferometry for deformation measurement 显示文摘 | MONSERRAT O CRIOSETTO M LUZI G | 2014 | Isprs Journal of Photogrammetry and Remote Sensing2014，93，： | 1 |
| 8 | Hybrid organic inorganic Nylon6/SiO2 nanocomposites:transport properties显示文摘 | Jonathan B Ramon E G | 2004 | Polym Eng & Sci2004，44，7： | 1 |
| 9 | METIS research advances towards the 5G mobile and wireless system definition显示文摘 | Monserrat JF Mange G Braun V | 2015 | Eurasip Journal on Wireless Communications&Networking2015，2015，1： | 1 |
| 10 | Using External Data in Operational Risk显示文摘 | JIM G MONSERRAT G JENS P N | 2007 | The Geneva Papers2007，，32： | 1 |
| 11 | Advanced Techniques for Dam Monitoring显示文摘 | Luzi G Crosetto M Monserrat O | 2010 | International Congress on Dam Maintenance and Rehabilitation Zaragoza Spain2010，2010，： | 1 |
| 12 | Oxidative dam- age:the biochemical mechanism of cellular injury and necro- sis in choline deficiency显示文摘 | Repetto M G Ossani G Monserrat A J | 2010 | Experimental and Molecular Pa- thology2010，88，1： | 1 |
| 13 | A Review of Ground-based SAR Interferometry for Deformation Measurement显示文摘 | Monserrat O Crosetto M Luzi G | 2014 | ISPRS Journal of Photogram- metry and Remote Sensing2014，93，7： | 1 |
| 14 | Toxicological effects of hepatotoxins(Microcystins) on aquatic organisms显示文摘 | G L L Pinho J S Yunes | 2003 | Comments on Toxicology2003，，9： | 1 |
| 15 | Association of atherosclerosis with expression of the LILRB1 receptor by human NK and T - ceils supports the infectious burden hypothesis 显示文摘 | NEUS R MONSERRAT F MONICA G | 2011 | AHA2011，11，5： | 1 |
| 16 | Large-scale extrusion processing and characterization of hybrid nylon-6/SiO2 nanocomposites 显示文摘 | Monserrat G Gerhard van V Mattijs G J | 2004 | Polym Adv Technol2004，15，： | 1 |
| 17 | Polyamide-6/silica nanocomposites显示文摘 | MONSERRAT G JAVIER G BEN N | 2004 | Adv Eng Material2004，6，9： | 1 |
| 18 | Hybrid organic inorganic nylon-6/Si O2nanocomposites:transport properties显示文摘 | Monserrat G Jonathan B Ramon E | 2004 | Polymer Engineering and Science2004，44，7： | 1 |
| 19 | Unusual clinical presentation of oral pyogenic granuloma with severe alveolar bone loss:A case report and review of literature显示文摘BACKGROUND Pyogenic granuloma(PG)is a localized,reddish and vascularized hyperplastic lesion of the connective tissue which occurs in the oral cavity.In most cases,the presence of this lesion does not show alveolar bone resorption.The pathology is diagnosed clinically with some caution.However,the diagnosis and treatment are usually corroborated with histopathological evidence.CASE SUMMARY Three clinical cases of PG associated with bone loss were described in this study.The three patients presented tumor-like growth which bled on touch,and were associated with local irritant factors.Radiographs showed bone loss.All cases were treated with conservative surgical excision.The scarring was satisfactory,and there was no case of recurrence.The diagnoses were based on clinical findings,and were confirmed histopathologically.CONCLUSION The occurrence of oral PG with bone loss is unusual.Therefore,clinical and radiographic evaluations are important for the diagnosis. | Sarah Monserrat LomelíMartínez Dennisse Bocanegra Morando Ana Esther Mercado González Juan Ramón Gómez Sandoval | 2023 | World Journal of Clinical Cases2023，11，16： | 0 |
| 20 | Microsurgical management of radicular cyst using guided tissue regeneration technique:A case report显示文摘BACKGROUND Radicular cyst is a lesion of odontogenic origin that arises from epithelial remains due to periapical periodontitis caused by inflammatory reactions generated at the apex of affected teeth with infected or necrotic pulps.The therapeutic mana gement of radicular cysts is controversial.There is only one case report of enucleation of a radicular cyst managed with microsurgery and apicoectomy,but without the use of the guided tissue regeneration(GTR)technique in the same surgical procedure.The present clinical case describes the management of a radicular cyst with microsurgical approach,performance of an apicoectomy of the tooth associated with the entity,application of GTR technique,use of a resorbable membrane of type I bovine collagen,and bovine xenograft.CASE SUMMARY A 68-year-old patient presented with a radicular cyst from an upper lateral incisor.The microsurgical management used was aimed at enucleating the chemical membrane,performing apicoectomy of the tooth along with careful and precise retrograde filling,and implementing GTR technique using a resorbable collagen membrane and bovine xenograft.The diagnosis of radicular cyst was confirmed using histopathological analysis.The patient underwent follow-up evaluations at 10 and 30 d postoperatively.At 4 months postoperative evaluation,she remained asymptomatic,and radiographs showed significant periapical healing with adequate bone formation.CONCLUSION These results suggest that microsurgical management using the GTR technique with collagen membrane and xenograft,contributes to bone regeneration. | Juan Carlos Gómez Mireles Eugenia Karina Martínez Carrillo Katia Alcalá Barbosa Evangelina Gutiérrez Cortés Javier González Ramos Luis Antonio González Gómez Rubén Alberto Bayardo González Sarah Monserrat Lomelí Martínez | 2024 | World Journal of Clinical Cases2024，12，7： | 0 |
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