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| 1 | Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma显示文摘AIM:To investigate cell type specific distribution ofβ-actin expression in gastric adenocarcinoma and its correlation with clinicopathological parameters.METHODS:β-actin is a housekeeping gene,frequently used as loading control,but,differentially expresses in cancer.In gastric cancer,an overall increased expression ofβ-actin has been reported using tissue disruptive techniques.At present,no histological data is available to indicate its cell type-specific expression and distribution pattern.In the present study,we analyzedβ-actin expression and distribution in paired normal and tumor tissue samples of gastric adenocarcinoma patients using immunohistochemistry(IHC),a tissue non-disruptive technique as well as tissue disruptive techniques like reverse transcriptase-polymerase chain reaction(RT-PCR)and western blotting.Correlation ofβ-actin level with clinicopathological parameters was done using univariate analysis.RESULTS:The results of this study showed significant overexpression,at both mRNA and protein level in tumor tissues as confirmed by RT-PCR(1.47±0.13 vs2.36±0.16;P<0.001)and western blotting(1.92±0.26 vs 2.88±0.32;P<0.01).IHC revealed thatβ-actin expression is majorly distributed between epithelial and inflammatory cells of the tissues.Inflammatory cells showed a significantly higher expression compared to epithelial cells in normal(2.46±0.13 vs 5.92±0.23,P<0.001),as well as,in tumor tissues(2.79±0.24 vs6.71±0.14,P<0.001).Further,comparison of immunostaining between normal and tumor tissues revealed that both epithelial and inflammatory cells overexpressβ-actin in tumor tissues,however,significant difference was observed only in inflammatory cells(5.92±0.23vs 6.71±0.14,P<0.01).Moreover,combined expression in epithelial and inflammatory cells also showed significant increase(4.19±0.15 vs 4.75±0.14,P<0.05)in tumor tissues.In addition,univariate analysis showed a positive correlation ofβ-actin level of inflammatory cells with tumor grade(P<0.05)while epithelial cells exhibited negative correlation(P>0.05).CONCLUSION:In gastric cancer,β-actin showed an overall higher expression predominantly contributed by inflammatory or tumor infiltrating immune cells of the tissue microenvironment and correlates with tumor grade. | Shafqat A Khan Monica Tyagi Ajit K Sharma Savio G Barreto Bhawna Sirohi Mukta Ramadwar Shailesh V Shrikhande Sanjay Gupta | 2014 | World Journal of Gastroenterology2014，20，34： | 2 |
| 2 | Identification and functional characterization of the candidate tumor suppressor gene TRIT1 in human lung cancer 显示文摘 | Monica S Antonella G Carmen P | 2005 | Oncogene2005，24，： | 1 |
| 3 | The brain-specific tissue-type plasminogen activator inhibitor, neuroserpin, protects neurons against excitotoxicity both in vitro and in vivo显示文摘 | Nathalie Lebeurrier Géraldine Liot José P. Lopez-Atalaya Cyrille Orset Monica Fernandez-Monreal Peter Sonderegger Carine Ali Denis Vivien | 2005 | Molecular and Cellular Neuroscience2005，，4： | 1 |
| 4 | Detecting chlorophyll Secchi disk depth and surfacee temperature in a sub-alpine lake using landsat irnagery显示文摘 | Claudia G Monica P Pietm A Paolo G Eugenio Z | 2001 | The Science of the Total Environment2001，268，： | 1 |
| 5 | Annual variation of airborne pollen in the city of Bahia,Argenting显示文摘 | Murray Maria G Sonaglioni Monica I Villamil Carlos B | 2002 | Grana2002，41，3： | 1 |
| 6 | Patterns of fruit set within inflorescence of Ancratiurn maritpiurn ( Amaryllidaceae ) : nouniform pollination, resource limitation, or architectural effects 显示文摘 | Monica M Pablo G Javier G | 2000 | American Journal of Botany2000，87，： | 1 |
| 7 | Biosynthesis and biochemical properties of the hepatitis C virus core protein显示文摘 | Santolini E Migliaccio G La Monica N | 1994 | J Virol1994，68，： | 1 |
| 8 | Interleukin 17A is dis- pensable for myocarditis but essential for the progression to dilat- ed cardiomyopathy显示文摘 | Christian B Jobert G Monica V | 2010 | Circ Res2010，106，10： | 1 |
| 9 | MgM ( M = Al and Ca) oxides as basic catalysts in transesterification processes 显示文摘 | Monica C G Albuquerque Jose Santamaria-Gonzalez Josefa M Merida-Robles | 2008 | Applied Catalysis A : General2008，347，2： | 1 |
| 10 | Optimum voltage control for loss minimization in HVDC multi- terminal transmission systems for large offshore wind farms显示文摘 | Monica A P Agusti E A Oriol G B | 2012 | Electric Power Systems Research2012，，89： | 1 |
| 11 | Microbial community variation and it's relationship with nitrogen mineralization in historically altered forests显示文摘 | Jennifer M F Teri C B Monica G T | 2006 | Ecology2006，87，3： | 1 |
| 12 | Electricity pricing under carbon emissions trading: A dominant firm with competitive fringe model显示文摘 | Monica B Frabcesco G | 2007 | Energy Policy2007，35，8： | 1 |
| 13 | Increased genetic diver- sity of BVDV-I: reeent findings and implications thereof显示文摘 | Monica G Letizia C Elisabetta R | 2015 | Virus Genes2015，50，： | 1 |
| 14 | Effect of exogenous DNA microinjection on early development response of the seabream(Sparus aurata)显示文摘 | Julia B Monica S | 1998 | Mol Mar Biol Biotechnol1998，7，4： | 1 |
| 15 | Adenine nucleotide translocase 3(ANT3) overexpression induces apoptosis in cultured cells显示文摘 | Monica Z Meritxell G Teresa M | 2004 | FEBS Letters2004，563，： | 1 |
| 16 | Spatial and temporal analysis of landscape patterns显示文摘 | Monica G Turner | 1999 | Landscape Ecology1999，4，1： | 1 |
| 17 | JNK1 controls mast cell degranulation and IL-1β production in inflammatory arthritis显示文摘 | Monica G Jun-ichi K Brian C | 2010 | Pnas2010，107，22： | 1 |
| 18 | Further studies on the interaction of the 5-Hydroxytryptamine3 (5-HT3) receptor with arylpiperazine ligands, development of a new 5-HT3 receptor ligand showing potent acetyleholinesterase inhibitory properties 显示文摘 | Andrea C Andrea G Monica M | 2005 | J Med Chem2005，48，10： | 1 |
| 19 | Reliability approach to service life prediction of concrete exposed to marine environment显示文摘 | MONICA P PHILIPPE G | 1996 | ACI Material Journal1996，93，6： | 1 |
| 20 | Shape and size of micelles in the sodium dodecyl sul fate-formamide system显示文摘 | CEGLIE A COLAFEMMINA G MONICA M D | 1993 | Langmuir1993，9，： | 1 |
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