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| 1 | Utility of serum CA19-9 in diagnosis of cholangiocarcinoma:In comparison with CEA显示文摘AIM:The diagnosis of cholangiocarcinoma is often difficult,making management approaches problematic. A reliable serum marker for cholangiocarcinoma would be a useful diagnostic test. The aims of our study were to evaluate the usefulness of a serum CA19-9 determination in the diagnosis of cholangiocarcinoma.METHODS: We prospectively measured serum CA19-9 and CEA concentrations in patients with cholangiocarcinoma (n=35), benign biliary diseases (n=92), and healthy individuals (n=15). Serum CA19-9 and CEA concentrations were measured by an immunoradiometric assay without knowledge of the clinical diagnosis.RESULTS:The sensitivity of a CA19-9 value>37KU·L^-1 and a CEA value >22μg·L^-1 in diagnosing cholangiocarcinoma were 77.14% and 68.57%, respectively. When compared with the benign biliary diseases group,the true negative rates of serum CA19-9 and CEA were 84.78% and 81.52%,respectively. The false positive rates of serum CA19-9 and CEA were 15.22% and 18.48%, whereas the accuracy of serum CA19-9 and CEA were 82.68% and 77.95%,respectively. Serum CA19-9 and CEA concentrations were significantly elevated (P<0.001 and P<0.05) in patients with cholangiocarcinoma (290.31±5.34KU·L^-1 and 36.46±18.03μg·L^-1) compared with patients with benign biliary diseases (13.38±2.59KU·L^-1 and 13.84±3.85μg·L^-1) and healthy individuals (12.78±3.69KU·L^-1 and 11.48±3.37μg·L^-1). In 15 patients undergoing curative resection of cholangiocarcinoma,the mean serum CA19-9 concentration was decreased from a preoperative level of 286.41±4.36KU·L^-1 to a postoperative level of 62.01±17.43KU·L^-1 (P<0.001), and the mean serum CEA concentration from 39.41±24.35μg·L^-1 to 28.69±11.03μg·L6-1(P<0.05). In patients with cholangiocarcinoma,however, no correlation was found between serum CEA and CA19-9 concentrations (r=-0.036).CONCLUSION:These data suggest that the serum CA19-9 determination is a useful addition to the available tests for the differential diagnosis of cholangiocarcinoma. Serum CA19-9 is an effective tumor marker in diagnosing cholangiocarcinoma,deciding whether the tumor has been radically resected and monitoring effect of treatment. | Xing-LeiQin Zuo-RenWang Jing-SenShi MinLu LinWang Quan-RuHe | 2004 | World Journal of Gastroenterology2004，10，3： | 40 |
| 2 | SynBioEcoli: a comprehensive metabolism network of engineered E. coil in three dimensional visualization显示文摘设计 E 的一个全面新陈代谢网络。coli 在系统生物学是很重要的, metabolomics 学习。许多工具集中于二维的空格在新陈代谢的网络显示小径。然而,三维的可视化的用法可以帮助更好理解新陈代谢、规章的网络的复杂拓扑学。方法我们手工地从文学的大量试验性的数据(包括的小径,反应和代谢物) 与设计 E 的不同类型联系了的 curated。coli 然后利用了三维的可视化的一种新奇技术开发一个全面新陈代谢的网络命名 SynBioEcoli。结果 SynBioEoli 包含 740 条 biosynthetic 小径, 3,889 新陈代谢的反应, 2,255 化学药品手工地从与设计 E 的不同类型联系的大约 11,000 份新陈代谢出版物加重 curated。coli。而且, SynBioEcoli 与各种各样的信息科学技术集成。结论 SynBioEcoli 能被认为是设计 E 的全面 knowledgebase。coli 并且代表下一代细胞的新陈代谢网络可视化技术。它能经由浏览器被存取(例如 Google 铬) 支持 WebGL,在 http://gffzz849681930f04473chouubp9qpqxu660oc.ffgz.tsg.suse.edu.cn/synbioecoli/ 。 | Weizhong Tu Shaozhen Ding Ling Wu Zhe Deng Hui Zhu Xiaotong Xu Chen Lin Chaonan Ye Minlu Han Mengna Zhao Juan Liu Zixin Deng Junni Chen Dong-Qing Wei Qian-Nan Hu | 2017 | Frontiers of Electrical and Electronic Engineering in China2017，5，1： | 2 |
| 3 | Expression of Hepatitis C Virus E2 Ectodomain in E.coli and Its Application in the Detection of Anti-E2 Antibodies in Human Sera显示文摘The second envelope glycoprotein (E2) of hepatitis C virus has been shown to bind human target cells and has become a major target for the development of anti-HCV vaccines. Anti-E2 antibodies have been suggested to be of clinical significance in diagnosis, treatment and prognosis of hepatitis C. However,large-scale expression and purification of E2 proteins in mammalian cells is difficult. As an alternative, E2 fragment (aa 385-730) with a four-amino-acid mutation (aa 568-571 PCNI to RVTS) was expressed as hexa-histidine-tagged full length protein [E2N730(m)] in E.coli and purified to over 85% purity. Purified E2N730(m) was specifically recognized by homologous hepatitis C patient serum in Western blot, suggesting that it displayed E2-specific antigenicity. Rabbit antiserum raised against E2N730(m) recognized E2 glycoproteins expressed in mammalian cells in Western blot. Purified E2N730(m) was ttsed to detect anti-E2 antibodies in human sera and showed better specificity and sensitivity than previously reported C-terminally truncated E2 fragment (aa 385-565). Association between anti-E2 antibodies in patient sera and HCV RNA status was also demonstrated using this E.coli-derived protein. E2N730(m) might serve as an inexpensive alternative to mammalian cell-expressed E2 proteins in clinical and research applications. | JingLIU Xin-XinZHANG Shen-YingZHANG MinLU Yu-YingKONG YuanWANG Guang-DiLI | 2004 | Acta Biochimica et Biophysica Sinica2004，36，1： | 2 |
| 4 | Endoplasmic Reticulum Retention, Degradation, and Aggregation of Olfactory G‐Protein Coupled Receptors显示文摘 | MinLu FernandoEcheverri Bryan D.Moyer | 2003 | Traffic2003，，6： | 1 |
| 5 | Preparation and properties of YBa 2 Cu 3 O 7? δ /Ag self-lubricating composites显示文摘 | Qiaodang Ding Changsheng Li Lirong Dong Minlu Wang Yi Peng Xuehua Yan | 2008 | Wear2008，，7： | 1 |
| 6 | Utility of a physiologically–based pharmacokinetic (PBPK) modeling approach to quantitatively predict a complex drug–drug–disease interaction scenario for rivaroxaban during the drug review process: implications for clinical practice显示文摘 | Joseph A.Grillo PingZhao JulieBullock Brian P.Booth MinLu KathyRobie‐Suh Eva GilBerglund K. SandyPang AtiqurRahman LeiZhang Lawrence J.Lesko Shiew‐MeiHuang | 2012 | Drug Dispos2012，，2： | 1 |
| 7 | Utility of a physiologically–based pharmacokinetic (PBPK) modeling approach to quantitatively predict a complex drug–drug–disease interaction scenario for rivaroxaban during the drug review process: implications for clinical practice显示文摘 | Joseph A.Grillo PingZhao JulieBullock Brian P.Booth MinLu KathyRobie‐Suh Eva GilBerglund K. SandyPang AtiqurRahman LeiZhang Lawrence J.Lesko Shiew‐MeiHuang | 2012 | Biopharm Drug Dispos2012，，2： | 1 |
| 8 | The Jinxi–Yelmand high-sulfidation epithermal gold deposit, Western Tianshan, Xinjiang Province, P.R. China显示文摘 | Xiao Long Nick Hayward Graham Begg Fu Minlu Wang Fangzheng Franco Pirajno | 2005 | Ore Geology Reviews2005，，1： | 1 |
| 9 | Pharmacokinetics of propafenone hydrochloride sustained-release capsules in male beagle dogs显示文摘This paper describes the development and validation of a liquid chromatography–mass spectrometric assay for propafenone and its application to a pharmacokinetic study of propafenone administered as a new propafenone hydrochloride sustained-release capsule(SR-test), as an instant-release tablet(IR-reference) and as the market leader sustained-release capsule(Rythmol, SR-reference) in male beagle dogs(n ? 8). In Study A comparing SR-test with IR-reference in a crossover design T_(max) and t_(1/2) of propafenone for SR-test were significantly higher than those for IR-reference while C_(max) and AUC were lower demonstrating the sustained release properties of the new formulation. In Study B comparing SRtest with SR-reference the observed C_(max) and AUC of propafenone for SR-test(124.57140.0 ng/m L and612.07699.2 ng·h/m L, respectively) were higher than for SR-reference(78.52772.92 ng/m L and423.67431.6 ng·h/m L, respectively) although the differences were not significant. Overall, the new formulation has as good if not better sustained release characteristics to the market leader formulation. | Liping Pan Yafang Qian Minlu Cheng Pan Gu Yanna He Xiaowen Xu Li Ding | 2015 | Acta Pharmaceutica Sinica B2015，5，1： | 1 |
| 10 | Cell division factor ZapE regulates Pseudomonas aeruginosa biofilm formation by impacting the pqs quorum sensing system显示文摘Pseudomonas aeruginosa is one of the leading nosocomial pathogens that causes both severe acute and chronic infections.The strong capacity of P.aeruginosa to form biofilms can dramatically increase its antibiotic resistance and lead to treatment failure.The biofilm resident bacterial cells display distinct gene expression profiles and phenotypes compared to their free-living counterparts.Elucidating the genetic determinants of biofilm formation is crucial for the development of antibiofilm drugs.In this study,a highthroughput transposon-insertion site sequencing(Tn-seq)approach was employed to identify novel P.aeruginosa biofilm genetic determinants.When analyzing the novel biofilm regulatory genes,we found that the cell division factor ZapE(PA4438)controls the P.aeruginosa pqs quorum sensing system.The ΔzapE mutant lost fitness against the wild-type PAO1 strain in biofilms and its production of 2-heptyl-3-hydroxy-4(1H)-quinolone(PQS)had been reduced.Further biochemical analysis showed that ZapE interacts with PqsH,which encodes the synthase that converts 2-heptyl-4-quinolone(HHQ)to PQS.In addition,site-directed mutagenesis of the ATPase active site of ZapE(K72A)abolished the positive regulation of ZapE on PQS signaling.As ZapE is highly conserved among the Pseudomonas group,our study suggests that it is a potential drug target for the control of Pseudomonas infections. | Xi Liu Minlu Jia Jing Wang Hang Cheng Zhao Cai Zhaoxiao Yu Yang Liu Luyan ZMa Lianhui Zhang Yingdan Zhang Liang Yang | 2023 | mLife2023，2，1： | 0 |
| 11 | Effect of CO_(2)-mixing dose and prolonged mixing time on fresh and hardened properties of cement pastes显示文摘This study aims to investigate the influence of CO_(2)-mixing dose(mass fractions of 0.3%,0.6%,and 0.9%)and prolonged mixing time on the fresh and hardened properties of cement pastes.The CO_(2)-mixing can act as coagulant in fresh cement mixtures,resulting in a significant reduction in workability associated with the formation of a rich calcium carbonate network on the surface of cement particles.The CO_(2)-mixing cement pastes were found to be much stiffer and more difficult to handle,place,and compact than the control mixture,which had a negative effect on the mechanical strength performance of the hardened pastes.However,prolonging the mixing time for 1 min(immediately after CO_(2)-mixing)can effectively improve the workability(by~53%–85%)by breaking up the flocculation network of deposited calcium carbonates.As a result,the presence of detached calcium carbonate accelerated early cement hydration and densified the microstructure;this improved early-age compressive strength by~6%–32%,depending on the CO_(2)-mixing dose used.Therefore,it seems that the CO_(2)-mixing dose should be controlled at≤0.6%with the mixing time prolonged in order to attain satisfactory workability and mechanical strength. | Minlu WANG Shuang LUO Ba Tung PHAM Tung-Chai LING | 2023 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2023，24，10： | 0 |
| 12 | Cytotoxicity,Degradation and Release Behavior of Alginate Fibers Loaded with Chlorhexidine Gluconate and Its Docking Simulation显示文摘To investigate the feasibility and safety of alginate fibers loaded with chlorhexidine gluconate(CHG)for biomedical applications,vitro cytotoxic test and drug release pattern were studied.Discovery Studio(DS),a computer aided software,was utilized to briefly investigate the energy state and solvation at the molecular level.Alginate fiber loaded with CHG presented different drug release patterns in PBS and A solution.Cytotoxicity results indicated that CHG-loaded alginate fibers can promote the growth of L-929 mouse fibroblast cells with better biocompatibility.Through the simulation,the lowest energy conformation of three representative molecular bond regions was optimized with stronger stability.As the torsional energy increased,the bond energy of the target regions decreased.Besides,the transition energy of the target regions was greatly reduced by solvation.In a word,alginate fibers loaded with CHG expressed better biocompatibility and drug sustained-release ability,which indicates that CHG-loaded alginate fibers has a broad application prospect in medical dressing. | Songsong Shi Yuting Chen Xinke Xia Yuan'e Zhang Minlu Feng Shengjiao Lei | 2022 | Journal of Harbin Institute of Technology(New Series)2022，29，5： | 0 |
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