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| 1 | AFF3-DNA methylation interplay in maintaining the mono-allelic expression pattern of XIST in terminally differentiated cells显示文摘X chromosome inactivation and genomic imprinting are two classic epigenetic regulatory processes that cause mono-allelic gene expression.In female mammals,mono-allelic expression of the long non-coding RNA gene X-inactive specific transcript(XIST)is essential for initiation of X chromosome inactivation upon differentiation.We have previously demonstrated that the central factor of super elongation complex-like 3(SEC-L3),AFF3,is enriched at gamete differentially methylated regions(DMRs)of the imprinted loci and regulates the imprinted gene expression.Here,we found that AFF3 can also bind to the DMR downstream of the XIST promoter.Knockdown of AFF3 leads to de-repression of the inactive allele of X IST in terminally differentiated cells.In addition,the binding of AFF3 to the XIST DMR relies on DNA methylation and also regulates DNA methylation level at DMR region.However,the KAP1-H3K9 methylation machineries,which regulate the imprinted loci,might not play major roles in maintaining the mono-aUelic expression pattern of XIST in these cells.Thus,our results suggest that the differential mechanisms involved in the X IST DMR and gDMR regulation,which both require AFF3 and DNA methylation. | Yue Zhang Chao Wang Xiaoxu Liu Qian Yang Hongliang Ji Mengjun Yang Manman Xu Yunyan Zhou Wei Xie Zhuojuan Luo Chengqi Lin | 2019 | Journal of Molecular Cell Biology2019，11，9： | 3 |
| 2 | Deformation behavior of dispersion-strengthened copper at high temperature显示文摘The deformation behavior of dispersion-strengthened copper with different compositions was investigated by hot compression simulation tests on a Gleeble-1500 thermal-mechanical simulator. The microstructure during deformation at high temperature was also studied. The result shows that at the beginning of hot compression simulation, the flowing stress of the dispersion-strengthened copper quickly attains a peak value and the stress shows a greater decrease when the tem- perature is higher and the strain rate is lower. The dispersion particles lead to an obvious increase in the recrystallization temperature. Under experimental conditions, dynamic recovery is the main softening method. The constitutive equation at high temperature of 1.2%Al2O3-0.4%WC/Cu is obtained. | WANG Mengjun ZHANG Yingchun LUO Yun LIU Xinyu | 2006 | Rare Metals2006，25，6： | 1 |
| 3 | Proscillaridin A inhibits hepatocellular carcinoma progression through inducing mitochondrial damage and autophagy显示文摘Hepatocellular carcinoma(HCC)is one of the leading causes of cancer-related deaths globally.At present,drug options for systemic treatment of HCC are very limited.There is an urgent need to develop additional effective drugs for HCC treatment.In the present study,we found that proscillaridin A(ProA),a cardiac glycoside,exerted a strong anticancer effect on multiple HCC cell lines.ProA significantly inhibited the cell proliferation,migration,and invasion of HCC cells.ProA also had a marked inhibitory effect on the progression of HCC in the MHCC97H xenograft nude mouse model.ProA-mediated suppression of HCC was closely related to cell apoptosis.ProA-treated HCC cells displayed significant mitochondrial damage and elevated reactive oxygen species production,resulting in profound cell apoptosis.Meanwhile,ProA also played a role in autophagy induction in HCC cells.Defects in autophagy partially relieved ProA’s anticancer effect in HCC cells.Our findings demonstrate that ProA can effectively inhibit HCC progression and may serve as a potential therapeutic agent for HCC treatment. | Mengjun Luo Yanfeng Liu Nannan Liu Weiqing Shao Lijun Ming Jing Liu Youhua Xie | 2021 | Acta Biochimica et Biophysica Sinica2021，53，1： | 1 |
| 4 | Establishment of an in vitro reporter system for screening HBx-targeting molecules显示文摘Chronic hepatitis B virus (HBV) infection remains a global public health problem. HBV-encoded X protein (HBx) is a multifunctional regulator that is required to initiate and maintain productive HBV infection, and is involved in HBV-related hepatocellular carci noma (HCC). Inhibitors that in terfere with the functions of HBx could be useful not only for the inhibition of HBV replication but also for the prevention or treatment of HBV-related HCC. To screen molecules that target HBx on a large scale remains a tech nical challenge due to a lack of sensitive and high-throughput system .In this work, we established an in vitro bioluminescent reporter system for screening HBx-targeting molecules. The system is based on a secretory fusion protein that combines HBx and NanoLuc (HBx-Nluc). The measured activity of NanoLuc in the culture supernatant of HBx-Nluc-expressing cells directly reflects the level of secreted HBx-Nluc. HBx protein-targeting intracellular anti-HBx single-chain variable fragment and RNA-targeting shRNA significantly reduced the secreted NanoLuc activity in HBx-Nluc-expressing cells. This system is simple and sensitive, and compatible with continuous non-disruptive screening, suggesting its potential usefulness for highthroughput screening and evaluating HBx-targeting molecules. | Chenjian Gu Shuai Tao Kongying Hu Lijun Ming Mengjun Luo Huimin Guo Yu Su Jing Liu Youhua Xie | 2019 | Acta Biochimica et Biophysica Sinica2019，51，4： | 0 |
| 5 | Unimpaired immunogenicity of yeast-expressed hepatitis B surface antigen stored at elevated temperatures显示文摘病毒(HBV ) 疫苗极大地减少了的肝炎 B 的全球采纳新 HBV 感染。当前的 HBV 疫苗是与铝磷酸盐或铝氢氧化物混合的液体暂停包含 recombinant 肝炎 B 表面抗原(HBsAg ) 粒子。制冷(2-8 楳湯? | Zhongliang Shen Yuliang Rao Shuai Tao Mengjun Luo Lijun Ming Jing Liu Shaokun Pan Youhua Xie | 2016 | Acta Biochimica et Biophysica Sinica2016，48，12： | 0 |
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