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| 1 | Signaling pathways and targeted therapy for myocardial infarction显示文摘Although the treatment of myocardial infarction(MI)has improved considerably,it is still a worldwide disease with high morbidity and high mortality.Whilst there is still a long way to go for discovering ideal treatments,therapeutic strategies committed to cardioprotection and cardiac repair following cardiac ischemia are emerging.Evidence of pathological characteristics in MI illustrates cell signaling pathways that participate in the survival,proliferation,apoptosis,autophagy of cardiomyocytes,endothelial cells,fibroblasts,monocytes,and stem cells.These signaling pathways include the key players in inflammation response,e.g.,NLRP3/caspase-1 and TLR4/MyD88/NF-κB;the crucial mediators in oxidative stress and apoptosis,for instance,Notch,Hippo/YAP,RhoA/ROCK,Nrf2/HO-1,and Sonic hedgehog;the controller of myocardial fibrosis such as TGF-β/SMADs and Wnt/β-catenin;and the main regulator of angiogenesis,PI3K/Akt,MAPK,JAK/STAT,Sonic hedgehog,etc.Since signaling pathways play an important role in administering the process of MI,aiming at targeting these aberrant signaling pathways and improving the pathological manifestations in MI is indispensable and promising.Hence,drug therapy,gene therapy,protein therapy,cell therapy,and exosome therapy have been emerging and are known as novel therapies.In this review,we summarize the therapeutic strategies for MI by regulating these associated pathways,which contribute to inhibiting cardiomyocytes death,attenuating inflammation,enhancing angiogenesis,etc.so as to repair and re-functionalize damaged hearts. | Qing Zhang Lu Wang Shiqi Wang Hongxin Cheng Lin Xu Gaiqin Pei Yang Wang Chenying Fu Yangfu Jiang Chengqi He Quan Wei | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 11 |
| 2 | Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing显示文摘Mammalian testis development is a complex and highly sophisticated process. To study the dynamic change of normal testis development at the transcriptional level, we investigated mouse testes at three postnatal ages: 6 days postnatal, 4 weeks old, and 10 weeks old, representing infant (PN1), juvenile (PN2), and adult (PN3) stages, respectively. Using ultra high-throughput RNA sequencing (RNA-seq) technology, we obtained 211 million reads with a length of 35 bp. We identified 18837 genes that were expressed in mouse testes, and found that genes expressed at the highest level were involved in spermatogenesis. The gene expression pattern in PN1 was distinct from that in PN2 and PN3, which indicates that spermatogenesis has commenced in PN2. We analyzed a large number of genes related to spermatogenesis and somatic development of the testis, which play important roles at different developmental stages. We also found that the MAPK, Hedgehog, and Wnt signaling pathways were significantly involved at different developmental stages. These findings further our understanding of the molecular mechanisms that regulate testis development. Our study also demonstrates significant advantages of RNA-seq technology for studying transcriptome during development. | GONG Wei PAN LinLin LIN Qiang ZHOU YuanYuan XIN ChengQi YU XiaoMin CUI Peng HU SongNian YU Jun | 2013 | Science China(Life Sciences)2013，56，1： | 8 |
| 3 | AFF3-DNA methylation interplay in maintaining the mono-allelic expression pattern of XIST in terminally differentiated cells显示文摘X chromosome inactivation and genomic imprinting are two classic epigenetic regulatory processes that cause mono-allelic gene expression.In female mammals,mono-allelic expression of the long non-coding RNA gene X-inactive specific transcript(XIST)is essential for initiation of X chromosome inactivation upon differentiation.We have previously demonstrated that the central factor of super elongation complex-like 3(SEC-L3),AFF3,is enriched at gamete differentially methylated regions(DMRs)of the imprinted loci and regulates the imprinted gene expression.Here,we found that AFF3 can also bind to the DMR downstream of the XIST promoter.Knockdown of AFF3 leads to de-repression of the inactive allele of X IST in terminally differentiated cells.In addition,the binding of AFF3 to the XIST DMR relies on DNA methylation and also regulates DNA methylation level at DMR region.However,the KAP1-H3K9 methylation machineries,which regulate the imprinted loci,might not play major roles in maintaining the mono-aUelic expression pattern of XIST in these cells.Thus,our results suggest that the differential mechanisms involved in the X IST DMR and gDMR regulation,which both require AFF3 and DNA methylation. | Yue Zhang Chao Wang Xiaoxu Liu Qian Yang Hongliang Ji Mengjun Yang Manman Xu Yunyan Zhou Wei Xie Zhuojuan Luo Chengqi Lin | 2019 | Journal of Molecular Cell Biology2019，11，9： | 3 |
| 4 | Characterization and Proteomic Analysis of Novel Rice Lesion Mimic Mutant with Enhanced Disease Resistance显示文摘Lesion mmic mutants(LMMs)are plants that spontaneously form lesions without pathogeninfection or external stimulus and exhibit resistance to pathogens.Here,a rice LMM was created by ethylmethane sulfonate mutagenesis,named as hpil(hydrogen peroxide induced lesion).Diaminobenzidineand trypan blue staining showed that large amounts of H_(2)O_(2) were produced and cell death was occurredat and around the parts of lesion mimic in the rice leaves.The phenotype of hpil is controlled by a singlerecessive gene,localized at a 2 Mb interval on chromosome 2.The data suggested that hpil is a novelLMM with enhanced bacterial and fungal disease resistance,and multiple pathogenesis-related proteins(PRs)were up-regulated.The proteomes of leaves at three positions(different degrees of lesion mimicseverity)were characterized in hpil compared with its wild type plant.Differentially expressed proteinswere detected by two dimensional difference gel electrophoresis and 274 proteins were identified byMALDITOF/TOFTM.These proteins were related to metabolic process,cellular process and response tostimulus,with mostly down-regulated in hpil leaves.Many of these proteins were related to the Calvincycle,photosynthetic electron transport chain,glycolysis/gluconeogenesis and phosphonates pathways.Some resistance-related proteins including 14-3-3 proteins,OsPR10 and antioxidases such asperoxidase,superoxide dismutase and ascorbate peroxidase were up-regulated in leaves with lesionmimic.These results provide the foundation for cloning of the target gene and shed light on themechanism involved in autaimmunity of rice. | Yang Yong Lin Qiujun Chen Xinyu Liang Weifang Fu Yuwen Xu Zhengjin Wu Yuanhua Wang Xuming Zhou Jie Yu Chulang Yan Chengqi Mei Qiong Chen Jianping | 2021 | Rice science2021，28，5： | 2 |
| 5 | Distribution,fractionation,and contamination assessment of heavy metals in offshore surface sediments from western Xiamen Bay,China显示文摘Surface sediment samples were collected at 21 offshore sites in western Xiamen Bay, Southeast China.Total concentrations of Li, V, Cr, Co, Ni, Cu, Zn, Sr, Mn,Pb, Ba, Fe, and Ti were determined by inductively coupled plasma-optical emission spectrometry; Hg was determined by atomic fluorescence spectrometry. A modified BCR sequential extraction procedure was used to extract fractions of the above elements. Concentrations of Pb, Cr, and Hg at most sites met the primary standard criteria of Marine Sediment Quality except site S12 for Pb and S7 for Cr, while concentrations of Zn at 17 sites and Cu at seven sites exceeded the criteria. The mean concentration of Hg was three times higher than the background, with a possible source being the Jiulong River. Fe, Ti, Ba, Co, V, and Li dominated the residual phase, mainly from terrestrial input.Ni, Cr, Pb, and Hg in the non-residual phase varied largely between sites. Sr, Mn, Cu, and Zn were mainly in the nonresidual fraction. Most sites showed considerable ecological risk; exceptions were site S7(very high) and sites S10,S11, and S14(moderate). Cu showed moderate-to-high pollution and Pb exhibited no-to-low pollution, while other metals had a non-pollution status according to their ratios of secondary phase to primary phase(RSP). Results of two assessment methods showed moderate pollution and a veryhigh ecological risk for Cu, Zn, Ni, and Cr at site S7, which might be due to the local sewage treatment plant. | Qiuli Yang Gongren Hu Ruilian Yu Haixing He Chengqi Lin | 2016 | Acta Geochimica2016，35，4： | 2 |
| 6 | Targeting cellular senescence prevents glucocorticoid-induced bone loss through modulation of the DPP4-GLP-1 axis显示文摘Dear Editor,Glucocorticoids(GCs)often cause detrimental side effects,including osteoporosis and osteonecrosis,with no specific drugs available to circumvent these problems.1 Cellular senescence plays an essential role in bone loss,2,3 where senescent cells release various factors,termed senescence-associated secretory phenotype(SASP),which influences neighboring cells and disrupts normal tissues.2 Glucocorticoids can induce senescence in bone cells in young mice;2 however,the effects of GCs on senescence and SASP during bone metabolism in adult mice are unclear. | Tiantian Wang Lin Yang Zejun Liang Lin Wang Feijing Su Xiangxiu Wang Xuanhe You Chengqi He | 2021 | Signal Transduction and Targeted Therapy2021，6，5： | 1 |
| 7 | Phase separation,transcriptional elongation control,and human diseases显示文摘Precise regulation of gene transcription is of great importance to development and diseases.Promoter-proximal transcriptional pause is a key and general mechanism to precisely control transcription in metazoans.Subsequent to transcription initiation and synthesis of a short RNA,RNA polymerase Ⅱ(Pol Ⅱ)usually pauses at the promoter-proximal regions,standing by for further signals to be released into the productive elongation stage.Fine regulation of Pol Ⅱ pausing and release is achieved by the concerted action of many negative and positive elongation factors,including the super elongation complex(SEC).Recent studies suggested that phase-separated assemblies of transcription regulatory complexes could provide a general biophysical basis for the dynamic regulation of transcription in response to various cellular needs,though direct evidence at endogenous level in living cells is still largely lacking.Here,we summarize and discuss latest advances in understanding how phase separation contributes to RNA polymerase ll-mediated transcription,with a focus on transcriptional pause and release. | Chenghao Guo Zhuojuan Luo Chengqi Lin | 2021 | Journal of Molecular Cell Biology2021，13，4： | 1 |
| 8 | Epitranscriptomic technologies and analyses显示文摘RNA can interact with RNA-binding proteins(RBPs),mRNA,or other non-coding RNAs(ncRNAs)to form complex regulatory networks.High-throughput CLIP-seq,degradome-seq,and RNA-RNA interactome sequencing methods represent powerful approaches to identify biologically relevant ncRNA-target and protein-ncRNA interactions.However,assigning ncRNAs to their regulatory target genes or interacting RNA-binding proteins(RBPs)remains technically challenging.Chemical modifications to mRNA also play important roles in regulating gene expression.Investigation of the functional roles of these modifications relies highly on the detection methods used.RNA structure is also critical at nearly every step of the RNA life cycle.In this review,we summarize recent advances and limitations in CLIP technologies and discuss the computational challenges of and bioinformatics tools used for decoding the functions and regulatory networks of ncRNAs.We also summarize methods used to detect RNA modifications and to probe RNA structure. | Xiaoyu Li Qiao-Xia Liang Jin-Ran Lin Jinying Peng Jian-Hua Yang Chengqi Yi Yang Yu Qiangfeng Cliff Zhang Ke-Ren Zhou | 2020 | Science China(Life Sciences)2020，63，4： | 1 |
| 9 | Effects of Pulsed Electromagnetic Fields on Bone Mass and Wnt/β-Catenin Signaling Pathway in Ovariectomized Rats显示文摘 | Jun Zhou Hongchen He Lin Yang Shiju Chen Hua Guo Lu Xia Huifang Liu Yuxi Qin Chuan Liu Xiaofei Wei Yujing Zhou Chengqi He | 2012 | Archives of Medical Research2012，，4： | 1 |
| 10 | The Association Between H3K4me3 and Antisense Transcription显示文摘Histone H3 lysine 4 trimethylation(H3K4me3) is well known to occur in the promoter region of genes for transcription activation.However,when investigating the H3K4me3 profiles in the mouse cerebrum and testis,we discovered that H3K4me3 also has a significant enrichment at the 3 0 end of actively transcribed(sense) genes,named as 3 0-H3K4me3.3 0-H3K4me3 is associated with 15% of protein-coding genes in both tissues.In addition,we examined the transcriptional initiation signals including RNA polymerase II(RNAPII) binding sites and 5 0-CAGE-tag that marks transcriptional start sites.Interestingly,we found that 3 0-H3K4me3 is associated with the initiation of antisense transcription.Furthermore,3 0-H3K4me3 modification levels correlate positively with the antisense expression levels of the associated sense genes,implying that 3 0-H3K4me3 is involved in the activation of antisense transcription.Taken together,our findings suggest that H3K4me3 may be involved in the regulation of antisense transcription that initiates from the 3 0 end of sense genes.In addition,a positive correlation was also observed between the expression of antisense and the associated sense genes with 3 0-H3K4me3 modification.More importantly,we observed the 3 0-H3K4me3 enrichment among genes in human,fruitfly and Arabidopsis,and found that the sequences of 3 0-H3K4me3-marked regions are highly conserved and essentially indistinguishable from known promoters in vertebrate.Therefore,we speculate that these 3 0-H3K4me3-marked regions may serve as potential promoters for antisense transcription and 3 0-H3K4me3 appear to be a universal epigenetic feature in eukaryotes.Our results provide a novel insight into the epigenetic roles of H3K4me3 and the regulatory mechanism of antisense transcription. | Peng Cui Wanfei Liu Yuhui Zhao Qiang Lin Feng Ding Chengqi Xin Jianing Geng Shuhui Song Fanglin Sun Songnian Hu Jun Yu | 2012 | Genomics, Proteomics & Bioinformatics2012，10，2： | 1 |
| 11 | Comparative Analyses of H3K4 and H3K27 Trimethylations Between the Mouse Cerebrum and Testis显示文摘The global features of H3K4 and H3K27 trimethylations(H3K4me3 and H3K27me3) have been well studied in recent years,but most of these studies were performed in mammalian cell lines.In this work,we generated the genome-wide maps of H3K4me3 and H3K27me3 of mouse cerebrum and testis using ChIP-seq and their high-coverage transcriptomes using ribominus RNA-seq with SOLiD technology.We examined the global patterns of H3K4me3 and H3K27me3 in both tissues and found that modifications are closely-associated with tissue-specific expression,function and development.Moreover,we revealed that H3K4me3 and H3K27me3 rarely occur in silent genes,which contradicts the findings in previous studies.Finally,we observed that bivalent domains,with both H3K4me3 and H3K27me3,existed ubiquitously in both tissues and demonstrated an invariable preference for the regulation of developmentally-related genes.However,the bivalent domains tend towards a 'winner-takes-all' approach to regulate the expression of associated genes.We also verified the above results in mouse ES cells.As expected,the results in ES cells are consistent with those in cerebrum and testis.In conclusion,we present two very important findings.One is that H3K4me3 and H3K27me3 rarely occur in silent genes.The other is that bivalent domains may adopt a 'winner-takes-all' principle to regulate gene expression. | Peng Cui Wanfei Liu Yuhui Zhao Qiang Lin Daoyong Zhang Feng Ding Chengqi Xin Zhang Zhang Shuhui Song Fanglin Sun Jun Yu Songnian Hu | 2012 | Genomics, Proteomics & Bioinformatics2012，10，2： | 1 |
| 12 | Novel tracking method for the drinking behavior trajectory of pigs显示文摘Identifying and tracking the drinking behavior of pigs is of great significance for welfare feeding and piggery management. Research on pigs’ drinking behavior not only needs to indicate whether the snout is in contact with the water fountain, but it also needs to establish whether the pig is drinking water and for how long. To solve target loss and identification errors, a novel method for tracking the drinking behavior of pigs based on L-K Pyramid Optical Flow (L-K OPT), Kernelized Correlation Filters (KCF), and DeepLabCut (DLC) was proposed. First, the feature model of the drinking behavior of a sow was established by L-K OPT. In addition, the water flow vector was used to determine whether the animal drank water and to demonstrate the details of the movements. Then, on the basis of the improved KCF, the relocation model of the sow’s snout was established to resolve the problem of tracking loss in the snout. Finally, the tracking model of piglets’ drinking behavior was established by DLC to build the mapping association between the pig’s snout and the drinking fountain. By using 200 episodes of drinking water videos (30-60 s each) to verify the method proposed in this study, the results are explained that 1) according to the two important drinking water indexes, the Down (−135°, −45°) direction feature and the V2 (>10 pixels) speed feature, the drinking time could be accurate to the frame level, with an error within 30 frames;2) The overlapping precision (OP) was 95%, the center location error (CLE) was 3 pixels, and the speed was 300 fps, which were all superior to other traditional algorithms;3) The optimal learning rate was 0.005, and the loss value was 0.0 002. The method proposed in this study realized accurate and automatic monitoring of the drinking behavior of pigs, which could provide reference for other animal behavior monitoring. | Chengqi Liu Haijian Ye Longhe Wang Shuhan Lu Lin Li | 2023 | International Journal of Agricultural and Biological Engineering2023，16，6： | 0 |
| 13 | Adolescent Mouse Takes on An Active Transcriptomic Expression During Postnatal Cerebral Development显示文摘Postnatal cerebral development is a complicated biological process precisely controlled by multiple genes. To understand the molecular mechanism of cerebral development, we compared dynamics of mouse cerebrum transcriptome through three developmental stages using high-throughput RNA-seq technique. Three libraries were generated from the mouse cerebrum at infancy, adolescence and adulthood, respectively. Consequently, 44,557,729(infancy), 59,257,530(adolescence) and 72,729,636(adulthood) reads were produced, which were assembled into15,344, 16,048 and 15,775 genes, respectively. We found that the overall gene expression level increased from infancy to adolescence and decreased later on upon reaching adulthood. The adolescence cerebrum has the most active gene expression, with expression of a large number of regulatory genes up-regulated and some crucial pathways activated. Transcription factor(TF) analysis suggested the similar dynamics as expression pro?ling, especially those TFs functioning in neurogenesis differentiation, oligodendrocyte lineage determination and circadian rhythm regulation. Moreover, our data revealed a drastic increase in myelin basic protein(MBP)-coding gene expression in adolescence and adulthood, suggesting that the brain myelin may be generated sincemouse adolescence. In addition, differential gene expression analysis indicated the activation of rhythmic pathway, suggesting the function of rhythmic movement since adolescence; Furthermore,during infancy and adolescence periods, gene expression related to axon repulsion and attraction showed the opposite trends, indicating that axon repulsion was activated after birth, while axon attraction might be activated at the embryonic stage and declined during the postnatal development. Our results from the present study may shed light on the molecular mechanism underlying the postnatal development of the mammalian cerebrum. | Wei Xu Chengqi Xin Qiang Lin Feng Ding Wei Gong Yuanyuan Zhou Jun Yu Peng Cui Songnian Hu | 2014 | Genomics, Proteomics & Bioinformatics2014，12，3： | 0 |
| 14 | Identification and characterization of multipotential stem cells in immortalized normal ovarian surface epithelial cells显示文摘The ovarian surface epithelium(OsE)is a single layer of squamous-to-cuboidal epithelial cells that experience repetitive ovulatory rupture and subsequent repair.However,the characteristics of human immortalized ovarian surface epithelial cells(IOSE80)remain elusive.This study aims to determine whether IOSE80 cells have the characteristics of stem cell proliferation and multilineage differentiation and their application in regenerative medicine.IOsE80 cells are sequenced by high-throughput transcriptome analysis,and 5 sets of public data are used to compare the differences between IosE8o cells and bone marrow mesenchymal stem cells,pluripotent stem cells,and oocytes in transcriptome profiling.The IOsE80 cells present a cobblestone-like monolayer and express the epithelial cell marker KRT18;the stem cell markers IFITM3,ALDH1A1,and VIM;lowly express stem cell marker LGR5 and germ cell markers DDX4 and DAZL.In addition,the GO terms'regulation of stem cell proliferation','epithelial cell proliferation',etc.,are significantly enriched(P<0.05).IOSE80 cells have the potential to act as mesenchymal stem cells to differentiate into adipocytes with lipid droplets,osteoblasts,and chondroblasts in vitro.IOSE80 cells express pluripotent stem cell markers,including OCT4,SSEA4,TRA-1-60,and TRA-1-81,and they can be induced into three germ layers in vitro.IOSE80 cells also form oocyte-like cells in vitro and in vivo.In addition,IOSE80 cells exhibit robust proliferation,migration,and ovarian repair functions after in vivo transplantation.This study de-monstrates that losE8o cells have the characteristics of pluripotent/multipotent stem cells,indicating their im-portant role in tissue engineering and regenerative medicine. | Lin Hou Hanqing Hong Wenjiao Cao Liutong Wei Lichun Weng Shuang Yuan Chengqi Xiao Qiuwan Zhang Qian Wang Dongmei Lai | 2024 | Acta Biochimica et Biophysica Sinica2024，56，2： | 0 |
| 15 | Effects of cervical low-frequency electrical stimulation with various waveforms and densities on body mass,liver and kidney function,and death rate in ischemic stroke rats显示文摘Low-frequency electrical stimulation has resulted in favorable effects in the treatment of post-stroke dysphagia.However,the safety of cervical low-frequency electrical stimulation remains unclear because of numerous nerves and blood vessels in the neck.In the present study,rats with ischemic stroke underwent low-frequency electrical stimulation,and systemic and local effects of electrical stimulation at different densities and waveforms were investigated.Electrical stimulation resulted in no significant effects on body mass,liver or kidney function,or mortality rate.In addition,no significant adverse reaction was observed,despite overly high intensity of low-frequency electrical stimulation,which induced laryngismus,results from the present study suggested that it is safe to stimulate the neck with a low-frequency electricity under certain intensities. | Yonghong Yang Chengqi He Lin Yang Qiang Gao Shasha Li Jing He | 2011 | Neural Regeneration Research2011，6，4： | 0 |
| 16 | Metatranscriptomic analysis of host response and vaginal microbiome of patients with severe COVID-19显示文摘Dear Editor,The pandemic of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)has become a global public health threat.Here we use a TRACE-seq-based metatranscriptomic analysis to compare host responses and vaginal microbiome of postmenopausal female patients with underlying severe COVID-19 disease with those of healthy females,thereby providing insights into the changes in the microenvironment of women's reproductive system. | Meng Xiao Bo Lu Rui Ding Xia Liu Xian Wu Yaqian Li Xudong Liu Lin Qiu Zhibo Zhang Jing Xie Yu Chen Dong Zhang Liting Dong Meiling Zhang Jinying Peng Hua Yang Timothy Kudihna Yingchun Xu Taisheng Li Chengqi Yi Lan Zhu | 2022 | Science China(Life Sciences)2022，65，7： | 0 |
| 17 | Skeleton extraction and pose estimation of piglets using ZS-DLC-PAF显示文摘The accurate identification of various postures in the daily life of piglets that are directly reflected by their skeleton morphology is necessary to study the behavioral characteristics of pigs.Accordingly,this study proposed a novel approach for the skeleton extraction and pose estimation of piglets.First,an improved Zhang-Suen(ZS)thinning algorithm based on morphology was used to establish the chain code mechanism of the burr and the redundant information deletion templates to achieve a single-pixel width extraction of pig skeletons.Then,body nodes were extracted on the basis of the improved DeepLabCut(DLC)algorithm,and a part affinity field(PAF)was added to realize the connection of body nodes,and consequently,construct a database of pig behavior and postures.Finally,a support vector machine was used for pose matching to recognize the main behavior of piglets.In this study,14000 images of piglets with different types of behavior were used in posture recognition experiments.Results showed that the improved algorithm based on ZS-DLC-PAF achieved the best thinning rate compared with those of distance transformation,medial axis transformation,morphology refinement,and the traditional ZS algorithm.The node tracking accuracy reached 85.08%,and the pressure test could accurately detect up to 35 nodes of 5 pigs.The average accuracy of posture matching was 89.60%.This study not only realized the single-pixel extraction of piglets’skeletons but also the connection among the different behavior body nodes of individual sows and multiple piglets.Furthermore,this study established a database of pig posture behavior,which provides a reference for studying animal behavior identification and classification and anomaly detection. | Chengqi Liu Haijian Ye Shuhan Lu Zhan Tang Zhao Bai Lei Diao Longhe Wang Lin Li | 2023 | International Journal of Agricultural and Biological Engineering2023，16，3： | 0 |
| 18 | RNF219 interacts with CCR4-NOT in regulating stem cell differentiation显示文摘Regulation of RNA stability plays a crucial role in gene expression control.Deadenylation is the initial rate-limiting step for the majority of RNA decay events.Here,we show that RING finger protein 219(RNF219)interacts with the CCR4-NOT deadenylase complex.RNF219-CCR4-NOT exhibits deadenylation activity in vitro.RNA-seq analyses identify some of the 2-cell-specific genes and the neuronal genes significantly downregulated upon RNF219 knockdown,while upregulated after depletion of the CCR4-NOT subunit CNOTIO in mouse embryonic stem(ES)cells.RNF219 depletion leads to impaired neuronal lineage commitment during ES cell differentiation.Our study suggests that RNF219 is a novel interacting partner of CCR4-NOT and required for maintenance of ES cell pluripotency. | Hao Du Chen Chen Yan Wang Yang Yang Zhuanzhuan Che Xiaoxu Liu Siyan Meng Chenghao Guo Manman Xu Haitong Fang Fengchao Wang Chengqi Lin Zhuojuan Luo | 2020 | Journal of Molecular Cell Biology2020，12，11： | 0 |
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