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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Screening for main components associated with the idiosyncratic hepatotoxicity of a tonic herb, Polygonum multiflorum显示文摘典型药用的植物的主要成分,蓼 multiflorum (用汉语的 Heshouwu ) ,那导致特质的肝损害仍然保持不清楚。我们的以前的工作证明了有 lipopolysaccharide (LPS ) 的无毒的剂量和 Heshouwu 的治疗学的剂量的 cotreatment 能在老鼠导致肝损害,而独唱治疗不能导致看得见的损害。在现在的工作,用成分大美人和敲门在里面策略,我们发现 Heshouwu 的乙醇醋酸盐(EA ) 摘录在对待 LPS 的老鼠显示了可比较的特质的 hepatotoxicity 到整个摘录。结果显示血浆丙氨酸 aminotransferase, aspartate aminotransferase,和肝 histologic 的重要举起变化,而另外的分开的部分没能导致肝损害。有另外的分开的部分的 EA 摘录的混合在对待 LPS 的老鼠导致了可比较的特质的 hepatotoxicity 到整个摘录。化学分析进一步揭示了那 2,3,5,4-tetrahydroxy trans-stilbene-2-O -- 配糖物(trans-SG ) 和它的 cis 异构体是在 EA 的二主要混合物摘录。而且,孤立的 cis- ,并且不它的 trans 异构体,显示的可比较的特质的 hepatotoxicity 到在对待 LPS 的老鼠的 EA 摘录。cis-SG 的更高的内容从与收集的将军相比与 Heshouwu 联系的实际的肝沉醉病人在 Heshouwu 白酒或准备被检测样品。另外,血浆 metabolomics 分析证明 cis-SG-disturbing 充实显著地不同于的小径在对待 LPS 的老鼠的 trans-SG。所有这些结果建议 cis-SG 仔细与 Heshouwu 的特质的 hepatotoxicity 被联系。认为 trans-SG 的 cis-trans 异构化被紫外光或日光调停,我们的调查结果为在药发现控制 photoisomerization 并且为 Heshouwu 和芪相关的药的临床的使用用作参考。 | Chunyu Li Ming Niu Zhaofang Bai Congen Zhang Yanling Zhao Ruiyu Li Can Tu Huifang Li Jing Jing Yakun Meng Zhijie Ma Wuwen Feng Jinfa Tang Yun Zhu Jinjie Li Xiaoya Shang Zhengsheng Zou Xiaohe Xiao Jiabo Wang | 2017 | Frontiers of Medicine2017，11，2： | 41 |
| 3 | The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis显示文摘Artemisia annua,通常已知的同样香甜的苦恼或 Qinghao,是到中国的一个灌木土著人并且长被用于药用的目的。A。annua 现在作为有势力抗疟药混合物的唯一的生来的来源全球性被栽培, artemisinin。这里,我们报导 A 的 1.74-gigabase 染色体的一个高质量的草稿集会。annua,高度异质接合,富于重复定序,并且包含 63 ? 226 编码蛋白质的基因,在定序的植物种类之中的最大的数字之一。我们发现了那,作为在 Asteraceae 的一些定序的染色体之一, A。annua 染色体包含对这大被子植物 clade 特定的很多基因。尤其是,扩大和编码涉及萜烯生合成的酶的基因的功能的多样化与 artemisinin biosynthetic 小径的进化一致。我们进一步由介绍那 A 的 transcriptome 揭示了。annua 发展了复杂 transcriptional 规章的网络位于 \O 下面 artemisinin 生合成。把转基因的 A 基于我们产生了的全面 genomic 和 transcriptomic 分析。artemisinin 高级的生产的 annua 线,它现在为大规模生产准备好了并且将从而帮助遇见增加 artemisinin 的全球需求的挑战。 | Qian Shen Lida Zhang Zhihua Liao Shengyue Wang Tingxiang Yan Pu Shi Meng Liu Xueqing Fu Qifang Pan Yuliang Wang Zongyou Lv Xu Lu Fangyuan Zhang Weimin Jiang Yanan Ma Minghui Chen Xiaolong Hao Ling Li Yueli Tang Gang Lv Yan Zhou Xiaofen Sun Peter E. Brodelius Jocelyn K.C. Rose Kexuan Tang | 2018 | Molecular Plant2018，11，6： | 47 |
| 4 | Assessing the Early Response of Advanced Cervical Cancer to Neoadjuvant Chemotherapy Using Intravoxel Incoherent Motion Diffusion-weighted Magnetic Resonance Imaging: A Pilot Study显示文摘 | Yan-ChunWang Dao-Yu Hu Xue-Mei Hu Ya-Qi Shen Xiao-Yan Meng Hao Tang Zhen Li | 2016 | Chinese Medical Journal2016，，6： | 34 |
| 5 | Immunological synergistic mechanisms of trans-/cis-stilbene glycosides in Heshouwu-related idiosyncratic liver injury显示文摘Heshouwu is a traditional non-toxic Chinese medicine commonly used in the clinical setting;however,clinical cases of Heshouwu-induced hepatotoxicity have been frequently reported^([1]).Pharmacoepidemiological studies have found that Heshouwu-induced liver injury occurs only in a small fraction of individuals taking the drug;these patients presented with the typ- | Lanzhi He Ping Yin Yakun Meng Jinfa Tang Tingting He Ming Niu Yuming Guo Yun Zhu Jing Jing Chunyu Li Zhijie Ma Jiabo Wang Zhaofang Bai Xiaohe Xiao | 2017 | Science Bulletin2017，62，11： | 29 |
| 6 | Isolation and Identification of Cancer Stem-Like Cells from Murine Melanoma Cell Lines显示文摘In current study,cancer stem-like cells in the murine melanoma B16F10 cells were investigated.CD phenotypes of the B16F10 cells were analyzed by flow cytometry,and the specific CD phenotype cells from the B16F10 cells were isolated by MACS.Then we used colony formation assay in soft agar media,the cell growth assay in serum-free culture media as well as the tumorigenicity investigation of the specific CD phenotype cells in C57BL/6 mice, respectively,to identify cancer stem-like cells in the B16F10 cells.The results showed that the B16F10 cells could form spherical clones in serum-free culture media,and the rate of clonegenesis of CD133 + ,CD44 + and CD44 + CD133 + cells was higher than that of CD133 - ,CD44 - and CD44 + CD133 - cells in soft agar media,respectively. The tumorigenic potential of CD133 + ,CD44 + ,CD44 + CD133 + cells and CD44 + CD133 + CD24 + cells was stronger than that of CD133 - ,CD44 - ,CD44 + CD133 - cells and CD44 + CD133 + CD24 - cells in mice,respectively.In conclusion,the CD44 + CD133 + CD24 + cells have some biological properties of cancer stem-like cells or are highly similar to the characteristics of cancer stem cells(CSC).These results provide an important method for identifying cancer stem-like cells in B16F10 cells and for further cancer target therapy. | Jun Dou Meng Pan Ping Wen Yating Li Quan Tang Lili Chu Fengshu Zhao Chuilian Jiang Weihua Hu Kai Hu Ning Gu | 2007 | Cellular & Molecular Immunology2007，4，6： | 25 |
| 7 | Comprehensive analysis of nitrogen distributions and ammonia nitrogen release fluxes in the sediments of Baiyangdian Lake, China显示文摘The condition of Baiyangdian Lake(BYDL) will improve as the Xiongan New Area evolves and fulfills its role of easing overcrowding and supporting economic growth. Water and sediment samples from BYDL were analyzed to provide information on nitrogen(N) contamination in BYDL. The mean ammonium N(NH_4^+-N), nitrate N, and total N concentrations in the water samples were 0.36, 0.12, and 2.22 mg/L, respectively, and the ranges were 0.003–8.38, 0.06–0.30,and 1.25–10.34 mg/L, respectively. The N concentrations in water gradually increased from the north to the south of BYDL. Sediment at 90% of the sampling sites was in or above the moderately contaminated class(1000–2000 mg/kg) defined in US Environmental Protection Agency total N pollution standards. Positive NH_4^+-N fluxes were found for 28 of the 34 sediment core samples, so the potential for NH_4^+-N being released from sediment was relatively high. The NH_4^+-N fluxes were 5.35–48.76 mg/m^2/day, and the mean and maximum fluxes were 8.71 and 48.76 mg/m^2/day, respectively. Benthic organisms will be affected more by NH_4^+-N and NH_3·H_2O in the surface sediment pore water(mean concentrations 4.93 and 0.13 mg/L, respectively) than by the other forms of N. | Yaoyao Zhu Xin Jin Wenzhong Tang Xin Meng Baoqing Shan | 2019 | Journal of Environmental Sciences2019，31，2： | 20 |
| 8 | The proliferation, differentiation, and mineralization effects of puerarin on osteoblasts in vitro显示文摘AIM: Osteoblasts are key functional cells in the process of bone metabolic balance. Phytoestrogens have an important influence on the proliferation and differentiation of osteoblasts. Puerarin, a plant estrogen, has a wide range concentration in vitro on the function of osteoblasts. The current study investigates the effect of the phytoestrogen puerarin on the proliferation, differentiation, and mineralization of osteoblasts in vitro. METHODS: The calvaria bone of eight-ten Wistar rats which were born within 24 h were obtained in aseptic condition. After enzyme digestion, isolation, purified osteoblasts of rats were cultured for further study. The cells of the first to third generation were divided into a control group and a puerarin-treated group with 10-3–10-10 mol·L-1 puerarin. The cells were exposed to the medium containing a low level of carbohydrates, 10%(V/V) FBS for 24 h. After 1 to 4 days of culture, the OD values on the proliferation of osteoblasts in each group were determined by microplate reader. The cells were cultured in the medium containing 50 μg·mL-1 vitamin C, 10-2 mol·L-1 sodium glycerophosphate, 10% FBS and the medium was changed every 3 to 4 days. After 2 to 8 days of culture, expression of alkaline phosphatase were tested and compared by microplate reader. The mineral nodes of osteoblasts were dyed using alizarin red or improved Von Kossa way after four weeks. RESULTS: Compared with those in the 10-5–10-9 mol·L-1 puerarin, the proliferation of osteoblasts, the expression of alkaline phosphatase, and the number of mineral nodes of osteoblasts were significantly decreased in the control group. The increase was the fastest in the third day, while on the fourth day it was decreased, and arrived at statistical significance compared with the alkaline phosphatase activities and control group. The 10-6 mol·L-1 group was the most distinct, and formed the most mineralized nodule. Compared with the 10-3 mol·L-1 puerarin group, those changes were markedly increased in the control group. CONCLUSIONS: Puerarin has proliferation, differentiation, and mineralization effects on osteoblasts in a dose-dependent manner, and has a double-way effect on the osteoblasts in vitro. A low-dose showed positive effects on the development of osteoblasts, and high-dose puerarin could inhibit the formation of bone. | WANG Chang MENG Mei-Xia TANG Xu-Lei CHEN Ke-Ming ZHANG Li LIU Wei-Ning ZHAO Ying-Yong | 2014 | Chinese Journal of Natural Medicines2014，12，6： | 18 |
| 9 | Sanguinarine Attenuates Lipopolysaccharide-induced Inflammation and Apoptosis by Inhibiting the TLR4/NF-KB Pathway in H9c2 Cardiomyocytes显示文摘 | Yan-yan MENG Yuan LIU Zhe-fu HU Yao ZHANG Jian NI Zhen-guo MA Hai-han LIAO Qing-qing WU Qi-zhu TANG | 2018 | Current Medical Science2018，38，2： | 18 |
| 10 | Submucosal tunneling endoscopic resection: An effective and safe therapy for upper gastrointestinal submucosal tumors originating from the muscularis propria layer显示文摘AIM To evaluate the effectiveness and safety of submucosal tunneling endoscopic resection(STER) and compare its outcomes in esophageal and cardial submucosal tumors(SMTs) of the muscularis propria(MP) layer.METHODS From May 2012 to November 2017, 173 consecutive patients with upper gastrointestinal(GI) SMTs of the MP layer underwent STER. Overall, 165 patients were included, and 8 were excluded. The baseline characteristics of the patients and SMTs were recorded. The en bloc resection rate, complete resection rate,residual rate, and recurrence rate were calculated to evaluate the effectiveness of STER, and the complication rate was recorded to evaluate its safety. Effectiveness and safety outcomes were compared between esophageal and cardial SMTs.RESULTS One hundred and twelve men and 53 women with a mean age of 46.9 ± 10.8 years were included. The mean tumor size was 22.6 ± 13.6 mm. Eleven SMTs were located in the upper esophagus(6.7%), 49 in the middle esophagus(29.7%), 46 in the lower esophagus(27.9%), and 59 in the cardia(35.7%). Irregular lesions accounted for 48.5% of all lesions. STER achieved an en bloc resection rate of78.7%(128/165) for GI SMTs with an overall complication rate of 21.2%(35/165).All complications resolved without intervention or were treated conservatively without the need for surgery. The en bloc resection rates of esophageal and cardial SMTs were 81.1%(86/106) and 72.1%(42/59), respectively(P = 0.142), and the complication rates were 19.8%(21/106) and 23.7%(14/59), respectively,(P =0.555). The most common complications for esophageal SMTs were gas-related complications and fever, while mucosal injury was the most common for cardial SMTs.CONCLUSION STER is an effective and safe therapy for GI SMTs of the MP layer. Its effectiveness and safety are comparable between SMTs of the esophagus and cardia. | Chen Du Ning-Li Chai En-Qiang Ling-Hu Zhen-Juan Li Long-Song Li Jia-Le Zou Lei Jiang Zhong-Sheng Lu Jiang-Yun Meng Ping Tang | 2019 | World Journal of Gastroenterology2019，25，2： | 17 |
| 11 | Phase 1 clinical trial demonstrated that MUC1 positive metastatic seminal vesicle cancer can be effectively eradicated by modified Anti-MUC1 chimeric antigen receptor transduced T cells显示文摘Recent progress in chimeric antigen receptor-modified T-cell(CAR-T cell) technology in cancer therapy is extremely promising, especially in the treatment of patients with B-cell acute lymphoblastic leukemia. In contrast, due to the hostile immunosuppressive microenvironment of a solid tumor, CAR T-cell accessibility and survival continue to pose a considerable challenge, which leads to their limited therapeutic efficacy. In this study, we constructed two anti-MUC1 CAR-T cell lines. One set of CAR-T cells contained SM3 single chain variable fragment(sc Fv) sequence specifically targeting the MUC1 antigen and co-expressing interleukin(IL) 12(named SM3-CAR). The other CAR-T cell line carried the SM3 sc Fv sequence modified to improve its binding to MUC1 antigen(named p SM3-CAR) but did not co-express IL-12. When those two types of CAR-T cells were injected intratumorally into two independent metastatic lesions of the same MUC1+ seminal vesicle cancer patient as part of an interventional treatment strategy, the initial results indicated no side-effects of the MUC1 targeting CAR-T cell approach, and patient serum cytokines responses were positive. Further evaluation showed that p SM3-CAR effectively caused tumor necrosis, providing new options for improved CAR-T therapy in solid tumors. | Fengtao You Licui Jiang Bozhen Zhang Qiang Lu Qiao Zhou Xiaoyang Liao Hong Wu Kaiqi Du Youcai Zhu Huimin Meng Zhishu Gong Yunhui Zong Lei Huang Man Lu Jirong Tang Yafen Li Xiaochen Zhai Xiangling Wang Sisi Ye Dan Chen Lei Yuan Lin Qi Lin Yang | 2016 | Science China(Life Sciences)2016，59，4： | 16 |
| 12 | Isoliquiritigenin inhibits TGF-β1-induced fibrogenesis through activating autophagy via PI3K/AKT/mTOR pathway in MRC-5 cells显示文摘Isoliquiritigenin(ISL),a natural flavonoid derived from the root of liquorice,has been reported to possess anti-inflammatory and antioxidant activities.Previous studies have found that ISL plays a crucial role in anti-fibrosis of adipose tissue and renal tissue;however,its effect on pulmonary fibrogenesis has not been demonstrated.In this study,we aimed to explore the roles and the underlying mechanisms of ISL in TGF-β1-induced fibrogenesis using human lung fibroblast-derived MRC-5 cells.Cell proliferation and migration were determined by MTT and wound healing assay,respectively.The expression levels of alpha-smooth muscle actin(α-SMA),collagen type I alpha 1(COLIA1)and fibronectin(FN),microtubule-associated protein light chain 3(LC3)and related signaling molecules were detected by quantitative real-time PCR,western blot and immunofluorescence assay,correspondingly.EGFP-LC3 transfection was used for autophagy analysis.The results showed that ISL inhibited the TGF-β1-induced proliferation and migration,and down-regulated the expressions ofα-SMA,COLIA1 and FN.ISL treatment led to up-regulation of LC3 in TGF-β1-treated MRC-5 cells,accompanied by significant decrease in the phosphorylation levels of phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),and mammalian target of rapamycin(mTOR).In addition,the inhibitory effects of ISL on TGF-β1-induced fibrogenic features in MRC-5 cells were enhanced by pretreatment with autophagy activator Rapmycin and PI3K/AKT inhibitor LY294002 and reversed by autophagy inhibitor 3-methyladenine and PI3K/AKT activator IGF-1.Taken together,our results demonstrated that ISL could attenuate the fibrogenesis of TGF-β1-treated MRC-5 cells by activating autophagy via suppressing the PI3K/AKT/mTOR pathway.Therefore,ISL holds a great potential to be developed as a novel therapeutic agent for the treatment of pulmonary fibrosis. | Jinjuan He Hao Peng Meifang Wang Ying Liu Xingrong Guo Bin Wang Longjun Dai Xueqin Cheng Zhongji Meng Leyong Yuan Fenglin Cai Yijun Tang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，8： | 16 |
| 13 | Reversal of B-cell hyperactivation and functional impairment is associated with HBsAg seroconversion in chronic hepatitis B patients显示文摘B 房间对再感染在肝炎 B 病毒(HBV ) 和保护的清理起一个重要作用。然而,与长期的 HBV 感染的结果被联系的这些房间的功能的特征仍然保持未知。我们包括地在不同阶段从 CHB 病人调查了外部 B 房间子集的频率,显型,和功能:有免疫力的忍耐(信息技术) ,有免疫力的激活(IA ) ,有免疫力的清理(IC ) ,有 HBsAg seroconversion 的应答者(解决的病人, RP ) ,并且健康控制(HC ) 。IA 病人与另外的组相比显示了外部血记忆 B 房间的更低的百分比。B 房间的全面 polyclonal 激活,由激活标记和 IgG 和 IgM 的分泌物的高水平显示了,在 IA 病人被观察。这 B 房间 hyperactivation 能被增加的 IFN-α 导致;并且在 IA 病人的可溶的 CD40 ligands。尤其是,合作激发器分子 CD80 和浆液 HBsAb 的表示和 HBsAg 特定的 B 房间的频率显著地与 HC 题目相比在信息技术, IA,和 IC 病人被减少。更重要地, B 房间 hyperactivation, co-stimulatory 分子 downregulation 和 HBsAg 特定的 B 房间缺陷在 RP 病人被颠倒。B 房间 hyperactivation 和功能的缺陷的颠倒在长期的肝炎 B 病人与 HBsAg seroconversion 被联系。 | Xiangsheng Xu Qinghua Shang Xinyue Chen Weimin Nie Zhengsheng Zou Ang Huang Ming Meng Lei Jin Ruonan Xu Ji-Yuan Zhang Junliang Fu Lifeng Wang Zirong Tang Yunbo Xie Xiaoming Yang Zheng Zhang Fu-Sheng Wang | 2015 | Cellular & Molecular Immunology2015，12，3： | 15 |
| 14 | Differential expression analysis of the broiler tracheal proteins responsible for the immune response and muscle contraction induced by high concentration of ammonia using iTRAQ-coupled 2D LC-MS/MS显示文摘Ammonia has been considered the contaminant primarily responsible for respiratory disease in poultry. Even though it can cause tracheal lesions, its adverse effects on the trachea have not been sufficiently studied. The present study investigated tracheal changes in Arbor Acres broilers(Gallus gallus) induced by high concentration of ammonia using isobaric tag for relative and absolute quantification(iTRAQ)-based proteome analysis. In total, 3,706 proteins within false discovery rate of 1% were identified, including 119 significantly differentially expressed proteins. Functional analysis revealed that proteins related to immune response and muscle contraction were significantly enriched. With respect to the immune response, up-regulated proteins(like FGA) were pro-inflammatory, while down-regulated proteins participated in antigen processing and antigen presenting(like MYO1G), immunoglobulin and cathelicidin production(like fowlicidin-2), and immunodeficiency(like PTPRC). Regarding muscle contraction, all differentially expressed proteins(like TPM1) were up-regulated. An over-expression of mucin, which is a common feature of airway disease, was also observed. Additionally, the transcriptional alterations of 6 selected proteins were analyzed by quantitative RT-PCR. Overall, proteomic changes suggested the onset of airway obstruction and diminished host defense in trachea after ammonia exposure. These results may serve as a valuable reference for future interventions against ammonia toxicity. | Yan Xiong Xiangfang Tang Qingshi Meng Hongfu Zhang | 2016 | Science China(Life Sciences)2016，59，11： | 15 |
| 15 | A Chromosome-Level Genome Assembly of Garlic (Allium sativum) Provides Insights into Genome Evolution and Allicin Biosynthesis显示文摘Garlic,an economically important vegetable,spice,and medicinal crop,produces highly enlarged bulbs and unique organosulfur compounds.Here,we report a chromosome-level genome assembly for garlic,with a total size of approximately 16.24 Gb,as well as the annotation of 57561 predicted protein-coding genes,making garlic the first Allium species with a sequenced genome.Analysis of this garlic genome assembly reveals a recent burst of transposable elements,explaining the substantial expansion of the garlic genome.We examined the evolution of certain genes associated with the biosynthesis of allicin and inulin neoseries-type fructans,and provided new insights into the biosynthesis of these two compounds.Furthermore,a large-scale transcriptome was produced to characterize the expression patterns of garlic genes in different tissues and at various growth stages of enlarged bulbs.The reference genome and large-scale transcriptome data generated in this study provide valuable new resources for research on garlic biology and breeding. | Xiudong Sun Siyuan Zhu Ningyang Li Yi Cheng Jing Zhao Xuguang Qiao Li Lu Shiqi Liu Yanzhou Wang Chan Liu Benping Li Wu Guo Shuang Gao Zemao Yang Fu Li Zheng Zeng Qing Tang Yupeng Pan Mengjiao Guan Jian Zhao Xiaomi ng Lu Huanwe n Meng Zhenlin Han Chun she ng Gao Wenkai Jiang Xing Zhao Shilin Tian Jianguang Su Zhihui Cheng Touming Liu | 2020 | Molecular Plant2020，13，9： | 14 |
| 16 | Chinese Medicine Sequential Therapy Improves Pregnancy Outcomes after Surgery for Endometriosis-Associated Infertility: A Multicenter Randomized Double-blind Placebo Parallel Controlled Clinical Trial显示文摘Objective:To evaluate the efficacy and safety of Chinese medicine(CM)improving pregnancy outcomes after surgery for endometriosis-associated infertility.Methods:A multicenter,randomized,doubleblind placebo parallel controlled clinical trial was designed.A total of 202 patients who had laparoscopy for endometriosis-associated infertility with qi stagnation and blood stasis syndrome were included and randomly divided into the CM treatment group and placebo control group at a ratio of 1:1 using a central block randomization from May 2014 to September 2017,101 patients in each group.The two groups received continuous intervention at 1–5 days after surgery,for 6 menstrual cycles.Before ovulation,the CM group was treated Huoxue Xiaoyi Granule(活血消异颗粒);after ovulation,Bushen Zhuyun Granule(补肾助孕颗粒)was involved.The control group was treated with placebo.Transvaginal ultrasonography was performed every menstrual cycle during the treatment,and female hormone levels in the follicular and luteal phases were measured during the 1 st,3 rd and 6 th menstrual cycles.The analysis was continued until pregnancy.The primary outcomes were clinical pregnancy rate and pregnancy outcome,and the secondary outcomes were follicular development and endometrial receptivity.Safety evaluations were performed before and after treatment.Results:(1)Clinical pregnancy and live birth rates:the clinical pregnancy and live birth rates of the CM group were significantly higher than those of the placebo group[44.6%(45/101)vs.29.7%(30/101),34.7%(35/101)vs.20.8%(21/101),both P<0.05].(2)Follicle development:the incidence of dominant follicles,rate of cumulative cycle ovulation,and rate of cumulative cycle mature follicle ovulation were significantly higher in the CM group than those in the placebo group[93.8%(350/373)vs.89.5%(341/381),80.4%(275/342)vs.69.1%(253/366),65.8%(181/275)vs 56.1%(142/253),P<0.05 or P<0.01]).The incidence of cumulative cycle luteinized unruptured follicle syndrome was significantly lower in the CM group than in the placebo group[11.7%(40/342)vs.17.8%(65/366),P<0.05).(3)Endometrial receptivity:after treatment,both endometrial types and endometrial blood flow types in the CM group were mainly types A and B,while those in the placebo group were mainly types B and C,with a significant difference between the two groups(both P<0.05).(4)Adverse events:the incidence of adverse events between the two groups was not significantly different(P>0.05).Conclusion:Strategies for activating blood circulation-regulating Gan(Liver)-tonifying Shen(Kidney)sequential therapy can effectively improve the clinical pregnancy rate and live birth rate of endometriosis-associated infertility with qi stagnation and blood stasis after laparoscopy,improve follicular development,promote ovulation,improve endometrial receptivity,while being a safe treatment option.(Trial registration No.NCT02676713). | ZHAO Rui-hua LIU Yong LU Dan WU Ying WANG Xiao-yun LI Wei-li ZENG Cheng MENG Qing-wei LIAN Feng-mei ZHOU Jun SHI Yun SUN Wei-wei HAN Qian TANG Yi SHI Guang | 2020 | Chinese Journal of Integrative Medicine2020，26，2： | 13 |
| 17 | Expression and alterations of different molecular form γ-glutamyl transferase and total RNA concentration during the carcinogenesis of rat hepatoma显示文摘INTRODUCTIONCarcinogenesisiscloselyrelatedwithDNAsynthesisandhypermetabolismofnucleicacid.γglutamyltransferase(GGT,EC2.3.2.2... | TANG Qi Yun, YAO Deng Fu, LU Jian Xin, WU Xin Hua and MENG Xian Yong | 1999 | World Journal of Gastroenterology1999，5，4： | 12 |
| 18 | Individual idea about the micro-invasive aspiration and drainage of intracranial hematoma显示文摘AIM: This study aimed to expound the individual idea of micro-invasive surgery from pre-operative preparation, intra-operative processing and post-operative management. METHODS: Pre-operative preparation was improved by analyzing pathological factors and hematoma property, and considering patients' age, basic disease, blood pressure control, with persistent haemorrhagia/rehaemorrhagia or not, operative occasion choice, positioning and other procedures. In the surgery, positioner was used. Initial aspiration volume was cautiously controlled. After operation, vital signs of patients were kept stable by cautiously using hematoma liquefacient and combining with free radical scavenger. RESULTS: The core content of individual micro-invasive surgery was mainly to relieve intracranial pressure. Under the condition of sufficient pre-operative preparation known by patients' family members, precise positioning was determined and individual therapeutic regimen was made. Meanwhile, caution should be taken in hematoma aspiration. Liquefaction and drainage should be paid more attention, and complications were processed actively. CONCLUSION: During the process of micro-invasive evacuation of intracranial hematoma for treating cerebral hemorrhage, attention should be paid to analyzing cerebral hematoma etiology and pathophysiological mechanism, and individual idea should be considered in surgical treatment aiming at patients' concrete disease condition. | Zhouping Tang Feng Xu Xingyong Chen Xiangwu Meng Wei Hu Suiqiang Zhu Wei Wang | 2007 | Neural Regeneration Research2007，2，12： | 12 |
| 19 | Water regime-nitrogen fertilizer incorporation interaction:Field study on methane and nitrous oxide emissions from a rice agroecosystem in Harbin, China显示文摘Water regime and nitrogen(N) fertilizer are two important factors impacting greenhouse gases(GHG) emission from paddy field, whereas their effects have not been well studied in cold region. In this study, we conducted a two-year field experiment to study the impacts of water regime and N fertilizer on rice yields and GHG emissions in Harbin, China, a cold region located in high latitudes. Our results showed that intermittent irrigation significantly decreased methane(CH_4) emission compared with continuous flooding, however,the decrement was far lower than the global average level. The N_2O emissions were very small when flooded but peaked at the beginning of the disappearance of floodwater. The N fertilizer treatments increased CH_4 emissions at low level(75 kg N/ha). But both CH_4 and N_2O emissions were uninfluenced at the levels of 150 kg N/ha and 225 kg N/ha. Rice yields increased under intermittent irrigation and were highest at the level of 150 kg N/ha. From our results, we recommended that the intermittent irrigation and 150 kg N/ha as the ideal water regime-nitrogen fertilizer incorporation for this area to achieve low GHG emissions without impacting rice yields. | Wenjun Dong Jia Guo Lijun Xu Zhifeng Song Jun Zhang Ao Tang Xijuan Zhang Chunxu Leng Youhong Liu Lianmin Wang Lizhi Wang Yang Yu Zhongliang Yang Yilei Yu Ying Meng Yongcai Lai | 2018 | Journal of Environmental Sciences2018，30，2： | 12 |
| 20 | Active injection protection scheme for flexible HVDC grids based on amplitude of input impedance显示文摘High-voltage direct current(HVDC)grids require fast and reliable protection of the DC lines.The performance of traditional protection schemes is easily impaired by the limitations of the boundary condition and nonlinearity from the control of converters.One of the key technologies for flexible HVDC grids is the half-bridge modular multilevel converter(HB-MMC).Considering the high controllability of HB-MMC,this study proposes an active injection protection scheme to improve the reliability and sensitivity of the HVDC grid protection.The HB-MMC is used to inject a sinusoidal characteristic signal,at the specified frequency,into the DC lines.Then,the voltage and current at the specified frequency are extracted using the Prony algorithm to calculate the input impedance,which is used for the identification of internal and external faults.The active injection protection scheme was simulated for various cases in the simulation software Power Systems Computer Aided Design.The simulation results indicate that the proposed protection scheme is highly reliable and can overcome transition resistance. | Jinghan He Keao Chen Meng Li Yong Tang Ming Nie | 2021 | Global Energy Interconnection2021，4，6： | 11 |
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