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| 1 | Maternal Eomesodermin regulates zygotic nodal gene expression for mesendoderm induction in zebrafish embryos显示文摘在在 midblastula 转变(MBT ) 的 zygotic 染色体激活前的动物胚胎的开发是实质上 supportedby 导出鸡蛋的母亲的产品。节的蛋白质在 MBT 以后是为中层和内胚叶正式就职的关键信号。Itremains 不清楚母亲的因素它在 zebrafish 胞胚的 ventrolateral blastodermal 边缘激活节的基因的 zygotic 表示。在这研究,我们显示出母亲的 Eomesodermina (Eomesa ) 的那损失,一个 T 盒子抄写因素,损害节的基因 ndr1 和 ndr2 以及 mesodermal 和 endodermal 标记的 zygotic 表示,在 mesendoderm 显示参与感应。母亲的 Eomesa 是为抄写因素基因 mxtx2 的及时 zygotic 表示的 alsorequired,节的基因表示的一个管理者。Eomesa 直接在激活他们的抄写的 ndr1, ndr2,和 mxtx2 的倡导者或 enhancer 绑在 Eomes 有约束力的地点。而且,节的基因也是的人和老鼠由 Eomes 调整了。进鼠科的胚胎的干细胞的 zebrafish eomesa 的 Transfection 与内长的节的表示的经常的高水平支持 mesendodermal 区别,建议 Eomes 的一个保存函数。一起拿,我们的调查结果在在脊椎动物胚胎调整节的基因表示和 mesendoderm 正式就职揭示母亲的 T-boxtranscription 因素的一个保存角色。 | Pengfei Xu Gaoyang Zhu Yixia Wang Jiawei Sun Xingfeng Liu Ye-Guang Chen Anming Meng | 2014 | Journal of Molecular Cell Biology2014，8，4： | 5 |
| 2 | A 2-bp insertion(c.6768insCC) in MC1R causes recessive white coat color in Bama miniature pigs显示文摘Coat color is an important characteristic of various breeds of domestic animal species.Variation in farm animal coat color is of considerable interest for concealment,communication and protection against solar radiation(Slominski et al.,2004).It also plays an important role in the regulation of physiological processes(Miyagi and Terai,2013).Bama miniature pigs are a native breed | Qitao Jia Chunwei Cao Hai Tang Ying Zhang Qiantao Zheng Xiao Wang Rui Zhang Xianlong Wang Ailing Luo Hong Wei Anming Meng Qi Zhou Hongmei Wang Jianguo Zhao | 2017 | Journal of Genetics and Genomics2017，44，4： | 5 |
| 3 | The Tiny Zebrafish Keep Swimming Fast in the Developmental Biology Pond显示文摘In 1996,the journal Development published a special issue on zebrafish solely focusing on characterization of dozens of phenotypic mutants chosen from hundreds of mutants identified through chemical(ENU)mutagenesis by two zebrafish groups in Tubingen and Boston.This milestone formally catapulted zebrafish to a league of genetically tractable | Jinrong Peng Jian Zhang Anming Meng | 2012 | Journal of Genetics and Genomics2012，39，9： | 2 |
| 4 | Tissue-specific expression of GFP reporter gene in germline driven by GATA-2 promoter and enhancers in zebrafish显示文摘GATA-2, a transcription factor, is expressed in several types of blood cells and in the central nervous system (CNS), and regulates the differentiation of these cells. We have obtained five zebrafish transgenic germlines that carry and express the green fluorescent protein (GFP) gene ligated to various 5’ flanking sepuences of zebrafish GATA-2 gene. The spatial pattern of GFP expression varies, mainly depending on which regulatory sequence is used, among the germlines. In some of the germlines, the expression of GFP is restricted to the CNS and the enveloping layer (EVL) cells, while in some other lines GFP is observed only in the CNS. it is noted that the intensity of GFP in the transgenic fish remain unchanged after a six-generation passage of the transgenes. The transgenic fish could find its uses in the future in generating tissue-specific, even cellspecific mutant fish and in functional study of related genes through transgenesis. | MENG Anming & LIN Shuo1. Laboratory of Developmental Biology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China 2. Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA | 2000 | Chinese Science Bulletin2000，45，1： | 2 |
| 5 | A Novel Zinc Finger Transcription Factor Resembles Krox - 20 in Structure and in Evpression Pattern in Zebrafish 显示文摘 | Zhihui Sun Kehui Shi Ying Su Anming Meng | | Mechanisms of Dev0，114，： | 1 |
| 6 | The DNA fingerprint generated by four fingerprint of poultry显示文摘 | Meng Anming QI Shunzhang GONG Guifen | 1993 | Progress in Biochemistry and Biophysics1993，20，2： | 1 |
| 7 | A zebrafish gene trap line expresses GFP recapturing expression pattern of foxj1b显示文摘Foxj1 has been found to play an important role in cilia formation and function in vertebrates. The zebrafish or Xenopus genome expresses two Foxj1 genes, foxj1a/FoxJ1 and foxj1b/FoxJ1.2. In this study, we have generated a zebrafish transgenic line T2BGSZ10 by Tol2 transposon-based gene trapping approach. T2BGSZ10 transgenic fish carry an insertion of the transposon genome into the first intron of the foxj1b locus. This insertion results in GFP expression in the forebrain, otic vesicles, floorplate, pronephric ducts and other domains during embryogenesis, which recaptures the expression pattern of foxj1b. Although normal expression of foxj1b is dramatically reduced, T2BGSZ10 homozygous embryos develop normally and grow to adulthood without detectable defects, which may be due to the incom-plete interruption of foxj1b expression. Nevertheless, this transgenic line may serve as a useful model for dynamic observation of GFP-labeled tissues and organs and for isolation of GFP-labeled cells. | Tian Tian Long Zhao Xinyi Zhao Min Zhang Anming Meng | 2009 | Journal of Genetics and Genomics2009，36，10： | 1 |
| 8 | Understanding recurrent pregnancy loss:recent advances on its etiology,clinical diagnosis,and management显示文摘Recurrent pregnancy loss(RPL)has become an important reproductive health issue worldwide.RPL affects about 2%–3%of reproductive-aged women,and makes serious threats to women’s physical and mental health.However,the etiology of approximately 50%of RPL cases remains unknown(unexplained RPL),which poses a big challenge for clinical management of these patients.RPL has been widely regarded as a complex disease where its etiology has been attributed to numerous factors.Heretofore,various risk factors for RPL have been identified,such as maternal ages,genetic factors,anatomical structural abnormalities,endocrine dysfunction,prethrombotic state,immunological factors,and infection.More importantly,development and applications of next generation sequencing technology have significantly expanded opportunities to discover chromosomal aberrations and single gene variants responsible for RPL,which provides new insight into its pathogenic mechanisms.Furthermore,based upon patients’diagnostic evaluation and etiologic diagnosis,specific therapeutic recommendations have been established.This review will highlight current understanding and recent advances on RPL,with a special focus on the immunological and genetic etiologies,clinical diagnosis and therapeutic management. | Chunwei Cao Shiyu Bai Jing Zhang Xiaoyue Sun Anming Meng Hui Chen | 2022 | Medical Review2022，2，6： | 1 |
| 9 | A harlequin ichthyosis pig model with a novel ABCA12 mutation can be rescued by acitretin treatment显示文摘Harlequin ichthyosis (HI) is a severe genetic skin disorder and caused by mutation in the ATP-binding cassette A12 (ABCA12) gene. The retinoid administration has dramatically improved long-term survival of HI, but improvements are still needed. However, the ABCA12 null mice failed to respond to retinoid treatment, which impedes the development of novel cure strategies for HI. Here we generated an ethylnitrosourea mutagenic HI pig model (named Z9), which carries a novel deep intronic mutation IVS49-727 A>G in the ABCA12 gene, resulting in abnormal mRNA splicing and truncated protein production. Z9 pigs exhibit significant clinical symptom as human patients with HI. Most importantly, systemic retinoid treatment significantly prolonged the life span of the mutant pigs via improving epidermal maturation, decreasing epidermal apoptosis, and triggering the expression of ABCA6. Taken together, this pig model perfectly resembles the clinical symptom and molecular pathology of patients with HI and will be useful for understanding mechanistic insight and developing therapeutic strategies. | Xiao Wang Chunwei Cao Yongshun Li Tang Hai Qitao Jia Ying Zhang Qiantao Zheng Jing Yao Guosong Qin Hongyong Zhang Ruigao Song Yanfang Wang Guanghou Shui Sin Man Lam Zhonghua Liu Hong Wei Anming Meng Qi Zhou Jianguo Zhao | 2019 | Journal of Molecular Cell Biology2019，11，12： | 1 |
| 10 | Induction of cranial and posterior trunk neural crest by exogenous retinoic acid in zebrafish显示文摘Retinoic acid (RA) plays an important role in development of vertebrate embryos. We demonstrate impacts of exogenous RA on the formation of neural crest cells in zebrafish using specific neural crest markers sox9b and crestin. Treatment with all-trans RA at 10-7 mmol/L at 50% epiboly induces sox9b expression in the forebrain and crestin expression in the forebrain and midbrain, resulting in significant increase of pigment cells in the head derived from the cranial neural crest. In addition, RA treatment induces expression of sox9b and crestin in the caudal marginal cells of the neuroectoderm during early segmentation. Earlier commitment of these cells to the neural crest fate in the posterior margins leads to abnormal development of the posterior body, probably by preventing mingling of ventral derived and dorsal-derived cells during the formation of the tailbud. | Ming Li Ying Su Anming Meng | 2002 | Chinese Science Bulletin2002，47，13： | 1 |
| 11 | prpf4 is essential for cell survival and posterior lateral line primordium migration in zebrafish显示文摘Prpf4(pre-mRNA processing factor 4),a key component of spliceosome,plays critical roles in pre-mRNA splicing and its mutations result in retinitis pigmentosa due to photoreceptor defects.In this study,we characterized a zebrafishharboring a Tol2 transposon-based gene trap cassette in the third intron of the prpf4 gene.Cells in the brain and spinal cord gradually undergo p53-dependent apoptosis after 28 hpf insuggesting that a widespread function of prpf4 in neural cell survival.In addition,prpf4 is essential for survival of posterior lateral line primordial(pLLP)cells.prpf4 deficiency perturbs Fgf,and chemokine signaling pathways and impairs pLLP migration.RNA-Seq analysis suggests that prpf4 deficiency may impair spliceosome assembly,leading to compensatory upregulation of core spliceosomal genes and alteration of pre-mRNA splicing.Taken together,our studies uncover an essential role of prpf4 in pre-m RNA splicing,cell survival and pLLP migration. | Yixia Wang Yanchao Han Pengfei Xu Shihui Ding Guangyuan Li Hongbin Jin Yaping Meng Anming Meng Shunji Jia | 2018 | Journal of Genetics and Genomics2018，45，8： | 1 |
| 12 | Protein Phosphatase 4 Cooperates with Smads to Promote BMP Signaling in Dorsoventral Patterning of Zebrafish Embryos显示文摘 | Shunji Jia Fangyan Dai Di Wu Xia Lin Cencan Xing Yu Xue Ying Wang Mu Xiao Wei Wu Xin-Hua Feng Anming Meng | 2012 | Developmental Cell2012，，5： | 1 |
| 13 | The rising zebrafish research in China:Meeting report of the 3rd Chinese Zebrafish Principal Investigator Meeting & the Inaugural Meeting of China Zebrafish Society显示文摘The zebrafish has become a very important animal model,not only for developmental biology and genetics,but also for disease modeling and drug discovery.Since the year of 2000,zebrafish research has been rapidly growing in China,and the laboratories using zebrafish as the main subject have been increasing dramatically(Xie et al.,2015).For example,the number of participants | Dongyuan Ma Yuanyuan Xue Yifan Zhang Yonghua Sun Anming Meng Feng Liu | 2016 | Journal of Genetics and Genomics2016，43，10： | 1 |
| 14 | Characterization and expression pattern of pouII1,a novel class Ⅱ POU gene in zebrafish显示文摘POU domain transcription factors that share a conserved DNA-binding domain, POU domain, are important regulators for the development of embryos in various animal species. A novel zebrafish POU domain gene, poulll has been cloned. The poullll cDNA is 2080 kb in length and encodes a putative polypeptide of 596 ammo acids. It is placed into class II POU family since it shares a high degree of homology with the known members of this family. Northern hybridization identifies a major transcript of approximately 2.1 kb that was present in embryos at the single-cell stage throughout 24 h postfertilization. The whole mount in situ hybridization shows that poulll transcripts are present in the single-cell embryos, strongly suggesting that these transcripts are of maternal origin. During early development of the embryos, poulll mRNA was ubiquitously distributed in all cells and tissues. The transcripts are gradually limited to brains and become completely undetectable by day 3. To our knowledge, poulll is the | LI Ming CAO Ying ZHAO Zhixing LIN Shuo MENG Anming | 2001 | Chinese Science Bulletin2001，46，18： | 1 |
| 15 | ABC model and floral evolution显示文摘The paper introduces the classical ABC model of floral development and thereafter ABCD, ABCDE and quartet models, and presents achievements in the studies on floral evolution such as the improved understanding on the relationship of reproductive organs between gnetophytes and angiosperms, new results in perianth evolution and identified homology of floral organs between dicots and monocots. The evo-devo studies on plant taxa at different evolutionary levels are useful to better understanding the homology of floral organs, and to clarifying the mysteries of the origin and sub-sequent diversification of flowers. | LI Guisheng, MENG Zheng, KONG Hongzhi, CHEN Zhiduan & LU Anming Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China Correspondence should be addressed to Meng Zheng (e-mail: zhmeng@ ns.ibcas.ac.cn) and Lu Anming (e-mail:anmin@ns.ibcas.ac.cn) | 2003 | Chinese Science Bulletin2003，48，24： | 1 |
| 16 | The genetic program Of oocytes can be modified in vivo in the zebrafish ovary显示文摘Oocytes,the irreplaceable gametes for generating a new organism,are matured in the ovary of living female animals.It is unknown whether any genetic manipulations can be applied to immature oocytes inside the living ovaries.As a proof-of-concept, we here demonstrate genetic amendments of zebrafish immature oocytes within the ovary.Oocyte microinjection in situ (OMIS) stimulates tissue repair responses,but some of the microinjected immature oocytes are matured,ovulated and fertilizable.By OMIS-mediated Cas9 approach,ntla and gata5 loci of oocytes arrested at prophase I of meiosis are successfully edited before fertilization.Through OMIS,high efficiency of biaUeUc mutations in single or multiple loci using Cas9/gRNAs allows immediate manifestation of mutant phenotypes in Fo embryos and multiple transgenes can co-express the reporters in Fo embryos with patterns similar to germline transgenic embryos.Furthermore,maternal knockdown of dnmt1 by antisense morphoino via OMIS results in a dramatic decrease of global DNA methylation level at the dome stage and causes embryonic lethalityprior to segmentation period.Therefore,OMIS opens a door to efficiently modify the genome and provides a possibility to re repair genetically abnormal oocytes in situ. | Xiaotong Wu Weimin Shen Bingjie Zhang Anming Meng | 2018 | Journal of Molecular Cell Biology2018，10，6： | 0 |
| 17 | Temporospatial inhibition of Erk signaling is required for lymphatic valve formation显示文摘Intraluminal lymphatic valves(LVs)and lymphovenous valves(LVs)are critical to ensure the unidirectional flow of lymphatic fluid.Morphological abnormalities in these valves always cause lymph or blood reflux,and result in lymphedema. | Yaping Meng Tong Lv Junfeng Zhang Weimin Shen Lifang Li Yaqi Li Xin Liu Xing Lei Xuguang Lin Hanfang Xu Anming Meng Shunji Jia | 2023 | Signal Transduction and Targeted Therapy2023，8，10： | 0 |
| 18 | The second polar body contributes to the fate asymmetry in the mouse embryo显示文摘The polar bodies(PBs)are extruded microcells during oocyte meiosis and generally regarded as inessentials for embryonic development.Therefore,PBs have been widely used as important materials for pre-implantation genetic diagnosis in human.Here we report that the second PB(PB2)in the mouse zygote may play roles in cell-fate specification and post-implantation development.A subset of mRNAs encoding pluripotency-related factors are enriched in PB2.Nascent proteins may be synthesized in PB2 after fertilization and transport from PB2 to the zygote before the two-cell stage.The PB2-attached blastomere(pbB)at the two-cell stage,compared to the other blastomere(npbB),likely contributes more descendants to the inner cell mass(ICM)lineage in the blastocyst.Removal of PB2 from the zygote or transient blockage of material exchange between PB2 and the zygote by nocodazole treatment appears to cause a loss of the ICM fate bias of pbB.PB2 removal or nocodazole treatment also results in abnormal post-implantation development.Injection of PB2 lysate into pbB of PB2-removed two-cell-stage embryos may reset the cell-fate preference and rescue post-implantation development.Our data collectively suggest that PB2 would demarcate the earliest cell-fate asymmetry of the mouse zygote and be required for post-implantation development. | Hongbin Jin Yang Han Huasong Wang J.Xiao He Li Weimin Shen Lin Zhang Luxi Chen Shunji Jia Ping Yuan Hui Chen Anming Meng | 2022 | National Science Review2022，9，7： | 0 |
| 19 | TGFβ1a regulates zebrafish posterior lateral line formation via Smad5 mediated pathway显示文摘The zebrafish sensory posterior lateral line(pLL)has become an attractive model for studying collective cell migration and cell morphogenesis.Recent studies have indicated that chemokine,Wnt/β-catenin,Fgf,and Delta-Notch signaling pathways participate in regulating pLL development.However,it remains unclear whether TGFβsignaling pathway is involved in pLL development.Here we report a critical role of TGFβ1 in regulating morphogenesis of the pLL primordium(pLLP).The tgfβ1a gene is abundantly expressed in the lateral line primordium.Knockdown or knockout of tgfβ1a leads to a reduction of neuromast number,an increase of inter-neuromast distance,and a reduced number of hair cells.The aberrant morphogenesis in embryos depleted of tgfβ1a correlates with the reduced expression of atoh1a,deltaA,and n-cadherin/cdh2,which are known important regulators of the pLLP morphogenesis.Like tgfβ1a depletion,knockdown of smad5 that expresses in the pLLP,affects pLLP development whereas overexpression of a constitutive active Smad5 isoform rescues the defects in embryos depleted of tgfβ1a,indicating that Smad5 mediates tgfβ1a function in pLLP development.Therefore,TGFβ/Smad5 signaling plays an important role in the zebrafish lateral line formation. | Cencan Xing Bo Gong Yu Xue Yanchao Han Yixia Wang Anming Meng Shunji Jia | 2015 | Journal of Molecular Cell Biology2015，7，1： | 0 |
| 20 | Differentially expressed gene in osteosarcoma cell lines with different metastatic potentials显示文摘Objective:To study the expression of osteosarcoma metastasis associated gene using a cDNA microarray,and screen new candidate genes related to the development,progress and osteosarcoma metastasis.Methods:Total RNA of a low metastatic osteosarcoma and a high metastatic osteosarcoma(M6 and M8 cell lines,respectively) was extracted,purified to mRNA and then reverse transcribed to cDNA.M6 was used as the experimental group and M8 as the control group,and the gene expression of cells from both of these two sublines was investigated using cDNA microarrays containig 8064 cDNA clones.The cDNA of M6 was labeled with cy3 and the cDNA of M8 was labeled with cy5.The two sublines were hybridized with the cDNA microarray.The hybridization signals were scanned with a Generation III array scanner and analyzed by Imagequant 5.0 software.Results:There were 330 differentially expressed genes between M6 and M8.In the M6 subline,152 genes were up-regulated and 178 genes were down-regulated compared to the M8 subline.These genes could be classified according to their function.Cell growth-related genes that were down-regulated included CCNG1,CDC2,APC10,and RPA3,while expression of the tumor suppressor genes,CDKN1A and CDKN2D,was up-regulated.Other genes that were differentially expressed included those that have been implicated in the regulation of signal transduction,metabolism and apoptosis.Conclusion:This study exploits a cDNA microarray approach to identifying genes that may be associated with metastasis.The gene expression profiles of osteosarcoma cell lines is a potentially important index in the search of new candidate genes related to tumor occurrence,development and metastasis. | Xinzhi Li Lin Meng Anming Chen Fengjin Guo Zhenqiang Luo Heng Zeng | 2009 | Journal of Nanjing Medical University2009，23，5： | 0 |
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