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| 1 | Effects of temperature and organic and inorganic nutrients on the growth of Chattonella marina (Raphidophyceae)from the Daya Bay,South China Sea显示文摘The effects of temperature and different forms of nutrients on Chattonella marina growth have been investigated in strains isolated from the Daya Bay,the South China Sea.The strain of C.marina preferred high temperatures, with an optimal temperature of 25°C,and 18 °C was the minimum for its survival.Higher cell number and growth rate were obtained in high nitrogen and phosphorus concentrations (500 μ g/L, 74 μ g/L) than under nutrient limitation.Nitrogen influenced the growth most, as the specific growth rate and maximum cell density were lower in nitrogen-limited cultures than noted under phosphorus limitation or under limitation from both.C.marina was capable of using many kinds of organic nitrogen sources including L-serine (L-Ser),glycine (Gly),alanine (Ala),L-threonine (L-Thr), glutamic acid (Glu) and urea, but could not utilize uric acid.Various forms of organic phosphorus compound such as glucose-6-phosphate (G6P),sodium glycerophosphate (GYP),adenosine triphosphate (ATP),adenosine monophosphate (AMP),cytidine monophosphate (CMP),guanosine monophosphate (GMP),uridine monophosphate (UMP),4-nitrophenylphosphate (NPP) and triethyl phosphate (TEP) supported the growth as well. Algal cells had the ability to sustain growth under nitrogen-and/or phosphorus-free conditions particularly under phosphorus depleted condition.These results led to the hypothesis that high loading of nitrogen has played an important role in frequent C.marina blooms in the past decade,and its capability for utilization of diverse forms of organic nutrients and growth in low nutrient conditions make this species a likely recurrent dominant in the Daya Bay phytoplankton assemblages,visible | WANG Zhaohui YUAN Meiling LIANG Yu LU Sollghui | 2011 | Acta Oceanologica Sinica2011，30，3： | 9 |
| 2 | In vitro expansion of human sperm through nuclear transfer显示文摘Dear Editor,Mammalian haploid embryonic stem cells(haESCs)represent an ideal tool for genetic analysis due to the presence of only one set of genetic materials.HaESCs fall into two readily distinguishable groups based on their genome origins:parthenogenetic haESCs(PG-haESCs)that are derived from oocyte-originated parthenogenetic embryos and androgenetic haESCs(AG-haESCs)that are produced through sperm nuclear transfer.Both PG-and AGhaESCs are feasible for delineating genome function at cellular level in vitro.Importantly,AG-haESCs can be used as a sperm replacement and applied to deciphering gene function at organismal level when combined with CRISPR-Cas9 technology.1 However,while PG-haESCs have been generated from both rodent and primates,2-5 AG-haESCs can only be obtained in rodent to date. | Xiaoyu Merlin Zhang Keliang Wu Yuxuan Zheng Han Zhao Junpeng Gao Zhenzhen Hou Meiling Zhang Jiaoyang Liao Jingye Zhang Yuan Gao Yuanyuan Li Lin Li Fuchou Tang Zi-Jiang Chen Jinsong Li | 2020 | Cell Research2020，30，4： | 3 |
| 3 | A necessary and sufficient condition of convexity for SOC reformulation of trust-region subproblem with two intersecting cuts显示文摘We consider the extended trust-region subproblem with two linear inequalities. In the 'nonintersecting' case of this problem, Burer and Yang(2015) have proved that its semi-definite programming relaxation with second-order-cone reformulation(SDPR-SOCR) is a tight relaxation. In the more complicated 'intersecting' case, which is discussed in this paper, so far there is no result except for a counterexample for the SDPR-SOCR. We present a necessary and sufficient condition for the SDPR-SOCR to be a tight relaxation in both the 'nonintersecting' and 'intersecting' cases. As an application of this condition, it is verified easily that the 'nonintersecting' SDPR-SOCR is a tight relaxation indeed. Furthermore, as another application of the condition, we prove that there exist at least three regions among the four regions in the trust-region ball divided by the two intersecting linear cuts, on which the SDPR-SOCR must be a tight relaxation. Finally, the results of numerical experiments show that the SDPR-SOCR can work efficiently in decreasing or even eliminating the duality gap of the nonconvex extended trust-region subproblem with two intersecting linear inequalities indeed. | YUAN JianHua WANG MeiLing AI WenBao SHUAI TianPing | 2016 | Science China Mathematics2016，59，6： | 2 |
| 4 | Copper‑Based Catalysts for Electrochemical Carbon Dioxide Reduction to Multicarbon Products显示文摘Electrochemical conversion of carbon dioxide into fuel and chemicals with added value represents an appealing approach to reduce the greenhouse effect and realize a carbon-neutral cycle,which has great potential in mitigating global warming and effectively storing renewable energy.The electrochemical CO_(2) reduction reaction(CO_(2)RR)usually involves multiproton coupling and multielectron transfer in aqueous electrolytes to form multicarbon products(C_(2+) products),but it competes with the hydrogen evolution reaction(HER),which results in intrinsically sluggish kinetics and a complex reaction mechanism and places higher requirements on the design of catalysts.In this review,the advantages of electrochemical CO_(2) reduction are briefly introduced,and then,different categories of Cu-based catalysts,including monometallic Cu catalysts,bimetallic catalysts,metal-organic frameworks(MOFs)along with MOF-derived catalysts and other catalysts,are summarized in terms of their synthesis method and conversion of CO_(2) to C2+products in aqueous solution.The catalytic mechanisms of these catalysts are subsequently discussed for rational design of more efficient catalysts.In response to the mechanisms,several material strategies to enhance the catalytic behaviors are proposed,including surface facet engineering,interface engineering,utilization of strong metal-support interactions and surface modification.Based on the above strategies,challenges and prospects are proposed for the future development of CO_(2)RR catalysts for industrial applications. | Fangfang Chang Meiling Xiao Ruifang Miao Yongpeng Liu Mengyun Ren Zhichao Jia Dandan Han Yang Yuan Zhengyu Bai Lin Yang | 2022 | Electrochemical Energy Reviews2022，5，3： | 2 |
| 5 | Monocyte–endothelial adhesion is modulated by Cx43-stimulated ATP release from monocytes显示文摘 | Dongdong Yuan Qin Wang Dengpan Wu Meiling Yu Suzhi Zhang Li Li Liang Tao Andrew L. Harris | 2012 | Biochemical and Biophysical Research Communications2012，，3： | 1 |
| 6 | Contemporary Asymmetric Genetic Introgression Between Two Pelophylax Species in Shanghai显示文摘Human disturbances are considered to break reproduction barriers among species.Significant increases in hybridization events have been reported among a large number of taxonomic groups in anthropogenic environments,providing novel insights into species evolution mechanisms and conservation management in the Anthropocene.The Eastern Golden Frog(Pelophylax plancyi)and BlackSpotted Frog(P.nigromaculatus)are two sympatric anuran species with a long history of mitochondrial genome introgression in highly urbanized continental East Asia.However,there is only limited understanding of the pattern of their contemporary hybridization and factors influencing their interspecific relationship under anthropogenic disturbances.Here,interspecific hybridization between P.plancyi and P.nigromaculatus at the population level was investigated in Shanghai.All except two haplotypes obtained from both species in Shanghai were mixed together,and located in the introgression clade,implying multiple ancient mitochondrial introgression events occurred in the populations of our study area.Asymmetric genetic introgression was detected by microsatellite markers,with 0.7%of P.plancyi and 14.6%of P.nigromaculatus identified as contemporary admixed individuals.Consistent with the trend of population density,higher genetic diversity of neutral microsatellite loci was found in the more abundant P.plancyi;however,variation in mitochondrial(Cyt-b)and nuclear(POMC)genes was higher in relatively rare P.nigromaculatus.The population density of P.plancyi and number of water patches within local habitats were significantly positively correlated with both occurrences and proportions of admixed individuals in the populations of P.plancyi and P.nigromaculatus.Considering the prevalent transformation of habitats in urbanized areas,these results imply that a high population density in isolated artificially altered habitats is likely to increase interspecific hybridization.Thus,population monitoring and improvement of landscape connectivity between habitats would be needed to control the intensity of interspecific hybridization between P.plancyi and P.nigromaculatus in anthropogenic-disturbed environments. | Xu WEI Wenli LIU Gang WANG Rui MA Meiling HUANG Jiaxin ZHENG Tingwei DONG Changqing YUAN Shunqi BO Xiao YUAN Ben LI Shuo MA Qu YUE Youzhong DING Zhenghuan WANG | 2024 | Asian Herpetological Research2024，15，1： | 0 |
| 7 | An engineered xCas12i with high activity,high specificity,and broad PAM range显示文摘Dear Editor,The clustered regularly interspaced short palindromic repeats-Cas(CRISPR-Cas)systems,including type II Cas9 and type V Cas12 systems,which serve in the adaptive immunity of prokaryotes against viruses,have been developed into genome-editing tools(Anzalone et al.,2020;Doudna,2020).Compared with type II systems,the type V systems including V-A to V-K showed more functional diversity(Yan et al.,2019).Amongst them,Cas12i has a relatively smaller size(1,033-1,093 aa),compared to SpCas9 and Cas12a,and has a 5'-TTN protospacer adjacent motif(PAM)preference(Yan et al.,2019). | Hainan Zhang Xiangfeng Kong Mingxing Xue Jing Hu Zikang Wang Yinghui Wei Haoqiang Wang Jingxing Zhou Weihong Zhang Mengqiu Xu Xiaowen Shen Fengcai Yin Zhiyuan Ai Guangyan Huang Junhui Xia Xueqiong Song Hengbin Li Yuan Yuan Jinhui Li Na Zhong Meiling Zhang Yingsi Zhou Hui Yang | 2023 | Protein & Cell2023，14，7： | 0 |
| 8 | A New Scheme Combining Phase Hopping with Differential Space-time Block Coding显示文摘 | HUANG Tao YUAN Chaowei LI Meiling ZHU Xiaolu | 2007 | Chinese Journal of Electronics2007，16，1： | 0 |
| 9 | A petunia transcription factor, PhOBF1, regulates flower senescence by modulating gibberellin biosynthesis显示文摘Flower senescence is commonly enhanced by the endogenous hormone ethylene and suppressed by the gibberellins(GAs)in plants.However,the detailed mechanisms for the antagonism of these hormones during f lower senescence remain elusive.In this study,we characterized one up-regulated gene PhOBF1,belonging to the basic leucine zipper transcription factor family,in senescing petals of petunia(Petunia hybrida).Exogenous treatments with ethylene and GA3 provoked a dramatic increase in PhOBF1 transcripts.Compared with wild-type plants,PhOBF1-RNAi transgenic petunia plants exhibited shortened f lower longevity,while overexpression of PhOBF1 resulted in delayed flower senescence.Transcript abundances of two senescence-related genes PhSAG12 and PhSAG29 were higher in PhOBF1-silenced plants but lower in PhOBF1-overexpressing plants.Silencing and overexpression of PhOBF1 affected expression levels of a few genes involved in the GA biosynthesis and signaling pathways,as well as accumulation levels of bioactive GAs GA1 and GA3.Application of GA3 restored the accelerated petal senescence to normal levels in PhOBF1-RNAi transgenic petunia lines,and reduced ethylene release and transcription of three ethylene biosynthetic genes PhACO1,PhACS1,and PhACS2.Moreover,PhOBF1 was observed to specifically bind to the PhGA20ox3 promoter containing a G-box motif.Transient silencing of PhGA20ox3 in petunia plants through tobacco rattle virus-based virus-induced gene silencing method led to accelerated corolla senescence.Our results suggest that PhOBF1 functions as a negative regulator of ethylene-mediated f lower senescence by modulating the GA production. | Xiaotong Ji Ziwei Xin Yanping Yuan Meiling Wang Xinyi Lu Jiaqi Li Yanlong Zhang Lixin Niu Cai-Zhong Jiang Daoyang Sun | 2023 | Horticulture Research2023，10，4： | 0 |
| 10 | Simultaneously achieving high energy density and responsivity in submicron BaTiO_(3) film capacitors integrated on Si显示文摘In the research field of energy storage dielectrics,the“responsivity”parameter,defined as the recyclable/recoverable energy density per unit electric field,has become critically important for a comprehensive evaluation of the energy storage capability of a dielectric.In this work,high recyclable energy density and responsivity,i.e.,W_(rec)=161.1 J·cm^(-3) and ξ=373.8 J·(kV·m^(2))^(-1),have been simultaneously achieved in a prototype perovskite dielectric,BaTiO_(3),which is integrated on Si at 500℃ in the form of a submicron thick film.This ferroelectric film features a multi-scale polar structure consisting of ferroelectric grains with different orientations and inner-grain ferroelastic domains.A LaNiO_(3) buffer layer is used to induce a{001}textured,columnar nanograin microstructure,while an elevated deposition temperature promotes lateral growth of the nanograins(in-plane diameter increases from~10-20 nm at lower temperatures to~30 nm).These preferably oriented and periodically regulated nanograins have resulted in a small remnant polarization and a delayed polarization saturation in the film’s P-E behavior,leading to a high recyclable energy density.Meanwhile,an improved polarizability/dielectric constant of the BaTiO_(3) film has produced a much larger maximum polarization than those deposited at lower temperatures at the same electric field,leading to a record-breaking responsivity for this simple perovskite. | Jun Ouyang Yinxiu Xue Chuanqi Song Meiling Yuan Kun Wang Yuyao Zhao Hongbo Cheng Hanfei Zhu Chao Liu | 2024 | Journal of Advanced Ceramics2024，13，2： | 0 |
| 11 | Inhibition of pyruvate dehydrogenase kinase improves carbohydrate utilization in Nile tilapia by regulating PDK2/4-PDHE1αaxis and insulin sensitivity显示文摘Pyruvate dehydrogenase kinases(PDKs)-pyruvate dehydrogenase E1αsubunit(PDHE1α)axis plays an important role in regulating glucose metabolism in mammals.However,the regulatory function of PDKsPDHE1α axis in the glucose metabolism of fish is not well known.This study determined whether PDKs inhibition could enhance PDHE1αactivity,and improve glucose catabolism in fish.Nile tilapia fingerlings(1.90±0.11 g)were randomly divided into 4 treatments in triplicate(30 fish each)and fed control diet without dichloroacetate(DCA)(38% protein,7% lipid and 45% corn starch)and the control diet supplemented with DCA,which inhibits PDKs through binding the allosteric sites,at 3.75(DCA3.75),7.50(DCA7.50)and 11.25 g/kg(DCA11.25),for 6 wk.The results showed that DCA3.75,DCA7.50 and DCA11.25significantly increased weight gain,carcass ratio and protein efficiency ratio(P<0.05)and reduced feed efficiency(P<0.05)of Nile tilapia.To investigate the effects of DCA on growth performance of Nile tilapia,we selected the lowest dose DCA3.75 for subsequent analysis.Nile tilapia fed on DCA3.75significantly reduced the mesenteric fat index,serum and liver triglyceride concentration and total lipid content in whole fish,and down-regulated the expressions of genes related to lipogenesis(P<0.05)compared to the control.The DCA3.75 treatment significantly improved glucose oxidative catabolism and glycogen synthesis in the liver,but significantly reduced the conversion of glucose to lipid(P<0.05).Furthermore,the DCA3.75 treatment significantly decreased the PDK2/4 gene and protein expressions(P<0.05),accordingly stimulated PDHE1αactivity by decreasing the phosphorylated PDHE1αprotein level.In addition,DCA3.75 treatment significantly increased the phosphorylated levels of key proteins involved in insulin signaling pathway and glycogen synthase kinase 3β(P<0.05).Taken together,the present study demonstrates that PDK2/4 inhibition by using DCA promotes glucose utilization in Nile tilapia by activating PDHE1αand improving insulin sensitivity.Our study helps to understand the regulatory mechanism of glucose metabolism for improving dietary carbohydrate utilization in farmed fish. | Yuan Luo Wenhao Zhou Ruixin Li Samwel MLimbu Fang Qiao Liqiao Chen Meiling Zhang Zhen-Yu Du | 2022 | Animal Nutrition2022，，4： | 0 |
| 12 | WDR74 functions as a novel coactivator in TGF-β signaling显示文摘Smads are critical intracellular signal transducers for transforming growth factor-β(TGF-β) in mammalian cells. In this study, we have identified WD repeat-containing protein 74(WDR74) as a novel transcriptional coactivator for Smads in the canonical TGF-β signaling pathway. Through direct interactions with Smad proteins, WDR74 enhances TGF-β-mediated phosphorylation and nuclear accumulation of Smad2 and Smad3. Consequently, WDR74 enables stronger transcriptional responses and more robust TGF-β-induced physiological responses. Our findings have elucidated a critical role of WDR74 in regulating TGF-β signaling. | Jinquan Liu Meiling Zhao Bo Yuan Shuchen Gu Mingjie Zheng Jian Zou Jianping Jin Ting Liu Xin-Hua Feng | 2018 | Journal of Genetics and Genomics2018，45，12： | 0 |
| 13 | Distribution Characteristics and Health Risk Assessment of Antimony in Atmospheric Particulates in a Northern City of China显示文摘Over the last several decades,scientists have established a wealth of evidence to demonstrate the risks posed to human health by toxic elements in atmospheric particulate matter(PM).Antimony(Sb),as one of ever ignored PM-bound heavy metals,attracts more and more attentions and has been regarded as one emerging air pollutant with the change of pollution sources of particulate matter.To study the distribution of Sb in PM with different particle sizes is of great practical significance for understanding its source and health risks.In this study,the size distributions of Sb in PM(PM_(2.5),PM_(10)and TSP)in different seasons were studied from July 2018 to May 2019.The high concentration of PM-bound Sb was found and the health risk was evaluated.Sb was enriched in fine particles and showed higher values in winter,which was probably caused by coal combustion and meteorological conditions.It was also found that traffic-related non-exhaust emissions might become another main contribution to fine particle Sb.Health risk assessment demonstrated that the hazard quotient(HQ)of Sb in PM_(2.5)and PM_(10)for children(PM_(2.5):1.54,PM_(10):1.32)exceeded the acceptable threshold and ingestion contributed the most to the HQ for both children and adults. | SHEN Yiwen ZHAO Hao ZHAO Changxian DONG Shuofei CAO Yuanming XIE Jiaojao LYU Meiling YUAN Chungang | 2023 | Chemical Research in Chinese Universities2023，39，3： | 0 |
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