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| 1 | Dyes adsorption using a synthetic carboxymethyl cellulose-acrylic acid adsorbent显示文摘Removal of noxious dyes is gaining public and technological attention. Herein grafting polymerization was employed to produce a novel adsorbent using acrylic acid and carboxymethyl cellulose for dye removal. Scanning electron microscopy and Fourier-transform infrared spectroscopy verified the adsorbent formed under optimized reaction conditions. The removal ratio of adsorbent to Methyl Orange, Disperse Blue 2BLN and malachite green chloride reached to 84.2%, 79.6% and 99.9%, respectively. The greater agreement between the calculated and experimental results suggested that pseudo second-order kinetic model better represents the kinetic adsorption data. Equilibrium adsorptions of dyes were better explained by the Temkin isotherm. The results implied that this new cellulose-based absorbent had the universality for removal of dyes through the chemical adsorption mechanism. | Genlin Zhang Lijuan Yi Hui Deng Ping Sun | 2014 | Journal of Environmental Sciences2014，26，5： | 9 |
| 2 | Simultaneously down-regulation of multiplex branch pathways using CRISPRi and fermentation optimization for enhancingβ-amyrin production in Saccharomyces cerevisiae显示文摘The production ofβ-amyrin in Saccharomyces cerevisiae is still low due to the inability of effectively regulating the endogenous metabolic pathway for competitive synthesis ofβ-amyrin precursors.In this study,we focused on two branches ofβ-amyrin synthetics pathway that consumeβ-amyrin precursors(2,3-oxidosqualene and cytosolic acetyl-CoA)and regulated related genes(ADH1,ADH4,ADH5,ADH6,CIT2,MLS2 and ERG7).We developed a CRISPRi method by constructing a multi-gRNA plasmid to down-regulate the seven genes simultaneously,which is reported for the first time in S.cerevisiae.The average transcription inhibition efficiency of the seven genes reached as high as 75.5%.Furthermore,by optimizing the fermentation condition(including pH,inoculum size,initial glucose concentration and feed of glucose or ethanol)and increasing extracellular transportation via supplying methyl-β-cyclodextrin,β-amyrin concentration of engineered strain SGibSdCg increased by 44.3%compared with the parent strain SGib,achieving 156.7 mg/L which was the highest concentration ofβ-amyrin reported in yeast.The one-step down-regulation of multiple genes using CRISPRi showed high efficiency and promising future in improving the yields of natural products. | Jiangping Ni Genlin Zhang Lei Qin Jun Li Chun Li | 2019 | Synthetic and Systems Biotechnology2019，4，2： | 4 |
| 3 | Copper-Catalyzed Allylic Oxidation of Cyclohexene with Molecular Oxygen显示文摘 | Xu Zhang Rong Yi Tian Chen Shichun Ni Genlin Wang Lei Yu | 2013 | 化学发展前沿(中英文版)2013，3，2： | 2 |
| 4 | Effects of extremely low frequency magnetic fields on gap junctional intercellular communication and its mechanism显示文摘The study on biological effect of electromagnetic fields has been paid close attention in recent years. Gap junctional intercellular communication (GJIC) plays an important role in the maintenance of cell proliferation and differentiation, and in the multistage process of carcinogenesis. A series of researches showed that xtremely low frequency (ELF) magnetic fields not only enhance the inhibition of GJIC induced by 12-O-tetradecanoylphorbol-13-acetate, but also inhibit GJIC directly when the intensity is equal to or more than 0.4 mT, and that the mechanisms of GJIC inhibition by ELF magnetic fields are due to hyperphosphorylation of connexin 43, which is mediated by protein kinase C-activated signal transduction, and the internalization of connexin 43 from plasma membrane to cytoplasm. | CHIANG Huai , HU Genlin and XU Zhengping(Microwave Laboratory, Zhejiang University School of Medicine, Hangzhou 310031, China) | 2002 | Progress in Natural Science:Materials International2002，12，3： | 2 |
| 5 | Genetic diversity of wild barley (Hordeum vulgare ssp. spontaneum) and its utilization for barley improvement显示文摘Wild barley(Hordeum vulgare spp.spontaneum) is the undisputed progenitor of cultivated barley and offers considerable potential as a genetic resource for barley improvement.Naturally occurring populations are distributed primarily across the Fertile Crescent,but secondary areas of diversity are also found in central Asia and Tibet.The pattern of its genetic diversity is non-random,and is structured on a global,regional and local scale.On a global scale,diversity follows ecogeography,while on a regional and local scale,it is more strongly determined by ecology.Populations growing in areas where the climate is unreliable and/or the soil is heterogeneous tend to retain the most diversity.A large number of accessions are currently conserved ex situ,but a high proportion of these have originated from the Fertile Crescent.Given the likelihood of future climate change,conserving germplasm both in situ and ex situ in the various regions where the species' currently existing is a research priority.The value of H.vulgare spp.spontaneum for the improvement of cultivated barley lies largely in its potential contribution to the levels of attainable pathogen resistance and abiotic stress tolerance. | XiaoYing Ma Chao Li AiDong Wang RuiJun Duan GenLin Jiao Eviatar Nevo GuoXiong Chen | 2012 | Research in Cold and Arid Regions2012，4，6： | 2 |
| 6 | LTSA-LE:A Local Tangent Space Alignment Label Enhancement Algorithm显示文摘According to smoothness assumption,local topological structure can be shared between feature and label manifolds.This study proposes a new algorithm based on Local Tangent Space Alignment(LTSA)to implement the label enhancement process.In general,we first establish a learning model for feature extraction in label space and use a feature extraction method of LTSA to guide the reconstruction of label manifolds.Then,we establish an unconstrained optimization model based on the optimal theory presented in this paper.The model is suitable for solving problems with a large number of sample points.Finally,the experiment results show that the algorithm can effectively improve the training speed and multilabel dataset prediction accuracy. | Chao Tan Genlin Ji Richen Liu Yanqiu Cao | 2021 | Tsinghua Science and Technology2021，26，2： | 2 |
| 7 | Optimization of preparation of activated carbon from cotton stalk by microwave assisted phos- phoric acid-chemical activation 显示文摘 | Hui Deng Genlin Zhang Xiaolin Xu | 2010 | Journal of Hazardous Materials2010，182，： | 1 |
| 8 | Fast conversion of glycerol to 1,3-propanediol by a new strain of Klebslella pneumoniae 显示文摘 | Zhang Genlin Ma Binbin Xu Xiaolin | 2007 | Biochem Eng J2007，37，3： | 1 |
| 9 | Research on the related properties of EVM/AI(OH) 3/SIO2 composites applied for halogen-free flame retardant cable insulation and jacket 显示文摘 | Lichun Wang Genlin Wang Pingkai Jiang | 2011 | Journal of Applied Polymer Science2011，120，： | 1 |
| 10 | Cloning and identification of magnetic field-responsive genes in Daudi cells显示文摘In order to study the molecular mechanisms of ELF MF biological effects, the differential display (DD) technology has been used to isolate differentially expressed cDNA fragments in Daudi cells after 50 Hz MF exposure and sham-exposure. Using total RNA isolated from 0.8 ml 50 Hz MF exposed cells and those from sham-exposed cells, one differentially regulated transcript has been found by DD and the expression pattern of the same transcript has been verified by reverse-Northern and Northern analyses. DNA sequence analysis and Genbank research demonstrated that the cDNA fragment (MF-CA) is a novel gene in human cell which is induced by ELF MF. | Ruiying Wu Huai Jiang Genlin Hu Yiti Fu Deqiang Lu | 2000 | Chinese Science Bulletin2000，45，11： | 1 |
| 11 | Mode I interlami- nar fracture property of moso bamboo ( Phyllostachys pubescens ) 显示文摘 | Shao Zhuoping Fang Changhua Tian Genlin | 2009 | Wood science and technology2009，43，56： | 1 |
| 12 | Optimization of preparation of activated carbon from cotton stalk by microwave assisted phosphoric acid-chemical activation显示文摘 | Deng Hui Zhang Genlin xu Xiaolin | | 0，，： | 1 |
| 13 | A review of researches on spatial clustering显示文摘 | LIU Sheng JI Genlin | 2010 | Journal of Nanjing Normal University(Engineering and Technology Edition)2010，，2： | 1 |
| 14 | Hybrid control of a parallel platform based on pneumatic artificial muscles combi- ning sliding mode controller and adaptive fuzzy CMAC 显示文摘 | SHI Genlin SHEN Wei | 2013 | Earthquake Engineering2013，21，1： | 1 |
| 15 | Bidirectionally catalytic polysulfide conversion by high-conductive metal carbides for lithium-sulfur batteries显示文摘Utilizing catalysts to accelerate the redox kinetics of lithium polysulfides (LiPSs) is a promising strategy to alleviate the shuttle effect of lithium–sulfur (Li–S) batteries.Nevertheless,most of the reported catalysts are only effective for LiPSs reduction,resulting in the devitalization of catalysts over extended cycles as a consequence of the continuous accumulation of Li_(2)S passivation layer.The situation gets even worse when employing mono-directional catalyst with poor electron conductivity because the charge transfer for the decomposition of solid Li_(2)S is severely hampered.Herein,a high-conductive and dualdirectional catalyst Co_(3)C decorated on porous nitrogen-doped graphene-like structure and carbon nanotube (Co_(3)C@PNGr-CNT) is fabricated as sulfur host,which not only promotes the precipitation of Li_(2)S from Li PSs during discharge but also facilitates the decomposition of Li;S during subsequent charge,as evidenced by the reduced activation energies for both reduction and oxidation processes.Furthermore,the long-term catalytic stability of Co_(3)C is corroborated by the reversible evolution of Co–C bond length over extended cycles as observed from X-ray absorption fine structure results.As a consequence,the fabricated Co_(3)C@PNGr-CNT/S cathode delivers a low capacity decay of 0.043%per cycle over 1000 cycles at 2C.Even at high sulfur loading (15.6 mg cm^(-2)) and low electrolyte/sulfur (E/S) ratio (~8μL mg^(-1))conditions,the battery still delivers an outstanding areal capacity of 11.05 m Ah cm^(-2) after 40 cycles.This work provides a rational strategy for designing high-efficient bidirectional catalyst with single component for high-performance Li-S batteries. | Genlin Liu Cheng Yuan Pan Zeng Chen Cheng Tianran Yan Kehua Dai Jing Mao Liang Zhang | 2022 | Journal of Energy Chemistry2022，31，4： | 1 |
| 16 | Fast conversation of glycerol to 1,3-propanediol by a new strain of klebsiella pneumonia显示文摘 | Zhang Genlin Ma Binbin Xu Xiaolin | 2007 | Biochemical Engineering Journal2007，37，3： | 1 |
| 17 | Flammability of EVA/IFR (APP/PER/ZB system) and EVA/IFR/synergist (CaCO<sub>3</sub>, NG, and EG) composites显示文摘 | Xinfeng Wu Lichun Wang Chao Wu Genlin Wang Pingkai Jiang | 2012 | J Appl Polym Sci2012，，6： | 1 |
| 18 | Situation and Reasons Analysis of the Children Lead Poisoning in China显示文摘 | QIN Genlin | 2000 | Chinese Journal of Shool Health (in Chinese)2000，21，3： | 1 |
| 19 | Polymorphism of the Promoter Region of HSP70 Gene and Its Relationship with the Expression of HSP70mRNA, HSF-ImRNA, Bcl-2mRNA and Bax-amRNA in Lymphocytes in Peripheral Blood of Heat Shock Dairy Cows 显示文摘 | YAFEI CAI QINGHUA LIU GENLIN WANG | 2005 | Asian-Aust J Anita Sci2005，18，5： | 1 |
| 20 | Construction of a eukaryotic expression plasmid for human retina-derived neurotrophin-3显示文摘Neurotrophin-3 (NT-3) can promote the repair of central nervous system and retinal damage. In previous reports, NT-3 has been expressed by viral vectors. However, plasmid vectors have a safer profile compared with viral vectors in clinical studies. This study recombined amplified human retinal NT-3 with a eukaryotic expression plasmid containing green fluorescent protein (GFP) to construct an NT-3 expression plasmid, pEGFP-N1-NT-3. The transfection efficiency 48 hours after pEGFP-N1-NT-3 transfection to 293T cells was 50.06 ± 2.78%. Abundant NT-3-GFP was expressed in 293T cells as observed by fluorescence microscopy, suggesting the construct pEGFP-N1-NT-3 effectively expressed and secreted NT-3-GFP. Secretory vesicles containing NT-3-GFP were observed in a constant location in cells by laser scan confocal microscopy, indicating the expression and secretion processes of NT-3 in eukaryotic cells were in accordance with the physical synthesis processes of secreted proteins. Western blot assay showed that pro-NT-3-GFP had a molecular weight of 56 kDa, further confirming NT-3-GFP expression. At 48 hours after transfection, the concentration of NT-3 in culture medium was 22.3 ng/mL, suggesting NT-3 produced by pEGFP-N1-NT-3 was efficiently secreted. This study constructed a human retinal-derived NT-3 eukaryotic expression plasmid that efficiently expressed and secreted NT-3. | Chunxia Peng Xiaobei Yin Mengda Li Ting He Genlin Li | 2013 | Neural Regeneration Research2013，8，11： | 1 |
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