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| 1 | Constitutive expression of feedback-insensitive cystathionine γ-synthase increases methionine levels in soybean leaves and seeds显示文摘Soybean(Glycine max(L.) Merr.) is a major crop that provides plant-origin protein and oil for humans and livestock.Although the soybean vegetative tissues and seeds provide a major source of high-quality protein,they suffer from low concentration of an essential sulfur-containing amino acid,methionine,which significantly limits their nutritional quality.The level of methionine is mainly controlled by the first unique enzyme of methionine synthesis,cystathione γ-synthase(CGS).Aiming to elevate methionine level in vegetative tissues and seeds,we constitutively over-expressed a feedback-insensitive Arabidopsis CGS(At D-CGS) in soybean cultivars,Zigongdongdou(ZD) and Jilinxiaoli 1(JX).The levels of soluble methionine increased remarkably in leaves of transgenic soybeans compared to wild-type plants(6.6-and 7.3-fold in two transgenic ZD lines,and 3.7-fold in one transgenic JX line).Furthermore,the total methionine contents were significantly increased in seeds of the transgenic ZD lines(1.5-to 4.8-fold increase) and the transgenic JX lines(1.3-to 2.3-fold increase) than in the wild type.The protein contents of the transgenic soybean seeds were significantly elevated compared to the wild type,suggesting that the scarcity of methionine in soybeans may limit protein accumulation in soybean seeds.The increased protein content did not alter the profile of major storage proteins in the seeds.Generally,this study provides a promising strategy to increase the levels of methionine and protein in soybean through the breeding programs. | YU Yang HOU Wen-sheng Yael Hacham SUN Shi WU Cun-xiang Ifat Matityahu SONG Shi-kui Rachel Amir HAN Tian-fu | 2018 | Journal of Integrative Agriculture2018，17，1： | 4 |
| 2 | Tip apex distance, hip screw placement, and neck shaft angle as potential risk factors for cut-out failure of hip screws after surgical treatment of intertrochanteric fractures显示文摘 | Hagen Andruszkow Michael Frink Cornelia Fr?mke Amir Matityahu Christian Zeckey Philipp Mommsen Stefanie Suntardjo Christian Krettek Frank Hildebrand | 2012 | International Orthopaedics2012，，11： | 2 |
| 3 | Does a volar locking plate provide equivalent stability as a dorsal non- locking plate in a dorsally comminuted distal radius frac- ture? a biomechanical study显示文摘 | Kandemir U Matityahu A Desai R | 2008 | J Orthop Trauma2008，22，9： | 1 |
| 4 | Superior gluteal artery injury during iliosacral screw placement due to aberrant anatomy 显示文摘 | Marmor M Lynch T Matityahu A | 2010 | Orthopedics2010，33，2： | 1 |
| 5 | How placement affects force and contact pressure between a volar plate of the distal radius and the flexor pollicus longus ten- don:a biomechanical investigation 显示文摘 | Matityahu AM Lapalme SN Seth A | 2013 | J Hand Surg Eur Vol2013，38，2： | 1 |
| 6 | Deter- minants of incident vulvovaginal candidiasis in human immunodeficiency vinls positive woman显示文摘 | Shifrin E Matityahu D Feldman J | 2010 | Infect Dis Obstet Gynecol2010，8，： | 1 |
| 7 | Superior gluteal artery injury during iliosacral screw placement due to aberrant anatomy 显示文摘 | Marmor M Lynch T Matityahu A | 2010 | Orthopedics2010，33，2： | 1 |
| 8 | Survivorship and severecomplications are worse for octogenarians and elderly patients withpelvis fractures as compared to adults:data from the nationaltrauma data bank显示文摘 | Matityahu A Elson J Morshed S | 2012 | J Osteoporos2012，2012，47： | 1 |
| 9 | Gene silencing by RNA interference in the white rot fungus Phanerochaete chrysosporium显示文摘 | Matityahu A Hadar Y Dosoretz CG | 2008 | Applied and Environmental Microbiology2008，74，17： | 1 |
| 10 | Superior gluteal artery injury during iliosacral screw placement due to aberrant anatomy显示文摘 | Marmor M Lynch T Matityahu A | 2010 | Orthopedics2010，33，2： | 1 |
| 11 | Does a volar locking plate provide equivalent stability as a dorsal nonlocking plate in adorsally comminuted distal radius fracture? 显示文摘 | KANDEMIR U MATITYAHU A DESAI R | 2008 | J Or- thop Trauma2008，22，9： | 1 |
| 12 | Over expression of mutated forms of aspartate kinase and cystathionine-y-synthase in tobacco leaves resulted in the high accumulation of methionine and threonine显示文摘 | Hacham Y Matityahu I Schuster G | 2008 | Plant Journal2008，54，2： | 1 |
| 13 | Histologic and Colposcopic Correlates of ASCUS Pap Smears in Pregnancy 显示文摘 | Broderick D Matityahu D Dudhbhai M | 2002 | J Low Genit Tract Dis2002，6，2： | 1 |
| 14 | Over expression of mutated forms of aspartat kinase and cystathioniney-synthase in tobacco leaves resulted in the high accumulation of methionine and threonine显示文摘 | Hacham Y Matityahu I Schuster G | 2008 | The Plant Journal2008，54，： | 1 |
| 15 | Soluble methionine enhances accumulation of a 15 kDa zein ,a methionine-rich storage protein,in transgenic alfalfa but not in transgenic tobacco plants显示文摘 | GOLAN A MATITYAHU I AVRAHAM T | 2005 | J Expe Bot2005，56，419： | 1 |
| 16 | Heterotopic ossification and acetabular fractures显示文摘 | MATITYAHU A BRUCK N MICLAU T | 2006 | Curr Opin Orthop2006，17，11： | 1 |
| 17 | Superior gluteal artery injury during iliosacral screw placement due to aberrant anatomy 显示文摘 | Marmor M Lynch T Matityahu A | 2010 | Orthopedics2010，33，2： | 1 |
| 18 | Tibial Plateau Fracture Repairs Augmented With Calcium Phosphate Cement Have Higher In Situ Fatigue Strength Than Those With Autograft显示文摘 | Erik McDonald Thomas Chu Michael Tufaga Meir Marmor Ravinder Singh Duran Yetkinler Amir Matityahu Jenni M Buckley R Trigg McClellan | 2011 | Journal of Orthopaedic Trauma2011，，2： | 1 |
| 19 | Prediction of postoper-ative knee flexion in Insall-Burstein II total knee arthroplasty显示文摘 | Schurman DJ Matityahu A Good SB | 1998 | Clin Orthop1998，353，： | 1 |
| 20 | Does a volar locking plate provide equivalent stability as a dorsal nonlocking plate in a dorsally comminuted distal radius fracture?A biomechanical study显示文摘 | Kandemir U Matityahu A Desai R | 2008 | J Orthop Trauma2008，22，9： | 1 |
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