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| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | Blood Lipid Profile and Prevalence of Dyslipidemia in Chinese Adults显示文摘Objective To investigate the plasma lipid levels in a national representative sample of subjects and to determine the prevalence of dyslipidemia in the Chinese population.Methods Plasma lipid profile was analyzed using the data obtained during the Chinese national nutrition and health survey(CNHS)in 2002 which involved 14 252 participants at the age of 18 years or older.Results The mean levels of total cholesterol(TC),triglyceride(TG)and high density lipoprotein cholesterol(HDL-C)in the participants were 3.81 mmol/L,1.10 mmol/L,and 1.30 mmol/L,respectively.In the groups of participants at the age of 18-44 years,45-59 years,and over 60 years the mean TC level was 3.70 mmol/L,4.09 mmol/L and 4.21 mmol/L,respectively,and the mean TG level was 07 mmol/L,1.21 mmol/L,1.20 mmol/L,1.29 mmol/L,1.33 mmol/L,and 1.33 mmol/L,respectively.The prevalence of dyslipidemia in Chinese adults was 18.6% and 22.2% in males and 15.9% in females.Dyslipidemia prevalence was higher in urban districts than in rural areas(21.0% vs.17.7%).The prevalence of hypercholesterolemia,hypertriglyceridemia,and low HDL cholesterol was 2.9%,11.9%,and 7.4% respectively among the participants.Conclusion Dyslipidemia has become one of the important health risk factors in the Chinese population.There is no significantly difference in the prevalence of dyslipidemia between the groups of participants at the age of 45-59 years and over 60 years.This study provides important lipid profile data for policy making and guideline development for the prevention of dyslipidemia in the Chinese population. | WEN-HUA ZHAO JIAN ZHANG YI ZHAI YUE YOU QING-QING MAN CHUN-RONG WANG HONG LI YING LI XIAO-GUANG YANG | 2007 | Biomedical and Environmental Sciences2007，20，4： | 22 |
| 3 | Acute cerebrovascular disease following COVID-19: a single center, retrospective, observational study显示文摘Background and purpose COVID-19 is an infectious disease caused by Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2).Apart from respiratory complications,acute cerebrovascular disease(CVD)has been observed in some patients with COVID-19.Therefore,we described the clinical characteristics,laboratory features,treatment and outcomes of CVD complicating SARS-CoV-2 infection.Materials and methods Demographic and clinical characteristics,laboratory findings,treatments and clinical outcomes were collected and analysed.Clinical characteristics and laboratory findings of patients with COVID-19 with or without new-onset CVD were compared.Results Of 219 patients with COVID-19,10(4.6%)developed acute ischaemic stroke and 1(0.5%)had intracerebral haemorrhage.COVID-19 with new onset of CVD were significantly older(75.7±10.8 years vs 52.1±15.3 years,p<0.001),more likely to present with severe COVID-19(81.8%vs 39.9%,p<0.01)and were more likely to have cardiovascular risk factors,including hypertension,diabetes and medical history of CVD(all p<0.05).In addition,they were more likely to have increased inflammatory response and hypercoagulable state as reflected in C reactive protein(51.1(1.3-127.9)vs 12.1(0.1-212.0)mg/L,p<0.05)and D-dimer(6.9(0.3-20.0)vs 0.5(0.1-20.0)mg/L,p<0.001).Of 10 patients with ischemic stroke;6 received antiplatelet treatment with aspirin or clopidogrel;and 3 of them died.The other four patients received anticoagulant treatment with enoxaparin and 2 of them died.As of 24 March 2020,six patients with CVD died(54.5%).Conclusion Acute CVD is not uncommon in COVID-19.Our findings suggest that older patients with risk factors are more likely to develop CVD.The development of CVD is an important negative prognostic factor which requires further study to identify optimal management strategy to combat the COVID-19 outbreak. | Yanan Li Man Li Mengdie Wang Yifan Zhou Jiang Chang Ying Xian David Wang Ling Mao Huijuan Jin Bo Hu | 2020 | Stroke & Vascular Neurology2020，5，3： | 22 |
| 4 | Brain tumor-targeted drug delivery strategies显示文摘Despite the application of aggressive surgery,radiotherapy and chemotherapy in clinics,brain tumors are still a difficult health challenge due to their fast development and poor prognosis.Brain tumor-targeted drug delivery systems,which increase drug accumulation in the tumor region and reduce toxicity in normal brain and peripheral tissue,are a promising new approach to brain tumor treatments.Since brain tumors exhibit many distinctive characteristics relative to tumors growing in peripheral tissues,potential targets based on continuously changing vascular characteristics and the microenvironment can be utilized to facilitate effective brain tumor-targeted drug delivery.In this review,we briefly describe the physiological characteristics of brain tumors,including blood–brain/brain tumor barriers,the tumor microenvironment,and tumor stem cells.We also review targeted delivery strategies and introduce a systematic targeted drug delivery strategy to overcome the challenges. | Xiaoli Wei Xishan Chen Man Ying Weiyue Lu | 2014 | Acta Pharmaceutica Sinica B2014，4，3： | 14 |
| 5 | Multicenter Evaluation of the Molecular Line Probe Assay for Multidrug Resistant Mycobacterium Tuberculosis Detection in China显示文摘In order to evaluate the performance of a molecular Hain line probe assay(Hain LPA)for rapid detection of rifampicin and isoniazid resistance of Mycobacterium tuberculosis in China。 | LI Qiang DONG Hai Yan PANG Yu XIA Hui OU Xi Chao ZHANG Zhi Ying LI Jun Chen ZHANG Jian Kang HUAN Shi Tong CHIN Daniel P KAM Kai Man ZHAO Yan Lin | 2015 | Biomedical and Environmental Sciences2015，28，6： | 13 |
| 6 | Blood perfusion of the contralateral testis evaluated with contrast-enhanced ultrasound in rabbits with unilateral testicular torsion显示文摘The changes of blood perfusion of contralateral testis after unilateral testicular torsion remain controversial.In this study,28 New Zealand white male rabbits were randomly divided into five groups.Group A(n=8),the control group,underwent a sham operation on the unilateral testis without inducing testicular torsion.In groups B,C,and D(n=5 each),unilateral testicular torsion was induced,and,after 3,6 or 24 h,respectively,detorsion was performed.In group E(n=5),permanent unilateral testicular torsion was applied.Contrast-enhanced ultrasound was used to observe the blood perfusion of the contralateral testis at the following stages:pre-torsion(preopration),immediately post-torsion(postopration),pre-detorsion,immediately post-detorsion,and late-stage post-detorsion(6-12 h post-detorsion in groups B-D)or at a similar time point(15-21 h post-torsion in group E).Time-intensity curves were generated,and the following parameters were derived and analyzed:arrival time,time to peak intensity,peak intensity,and half-time of the descending peak intensity.The analysis revealed that blood perfusion of the contralateral testis increased immediately after testicular torsion on the opposite side(P<0.05),which increased with prolonged testicular torsion of the other testis.This research demonstrated that contrast-enhanced ultrasound was valuable in evaluating blood perfusion of the contralateral testis after unilateral testicular torsion. | Lin Chen Wei-Wei Zhan Zhou-Jun Shen Wen-Bin Rui Chen Lv Man Chen Jian-Qiao Zhou Ping Zhou Mi Zhou Ying Zhu | 2009 | Asian Journal of Andrology2009，11，2： | 10 |
| 7 | Evolution of blood lipids and risk factors of dyslipidemia among people living with human immunodeficiency virus who had received first-line antiretroviral regimens for 3 years in Shenzhen显示文摘Background:Lipid abnormalities are prevalent among people living with human immunodeficiency virus(HIV)(PLWH)and contribute to increasing risk of cardiovascular events.This study aims to investigate the incidence of dyslipidemia and its risk factors in PLWH after receiving different first-line free antiretroviral regimens.Methods:PLWH who sought care at the Third People’s Hospital of Shenzhen from January 2014 to December 2018 were included,and the baseline characteristics and clinical data during the follow-up were collected,including total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C)and high-density lipoprotein cholesterol(HDL-C).The risk factors of dyslipidemia after antiretroviral therapy were analyzed with the generalized estimating equation model.Results:Among the 7623 PLWH included,the mean levels of TC,HDL-C and LDL-C were 4.23±0.85 mmol/L,1.27±0.29 mmol/L and 2.54±0.65 mmol/L,respectively,and the median TG was 1.17(IQR:0.85-1.68)mmol/L.Compared with that in PLWH receiving tenofovir disoproxil fumarate(TDF)+lamivudine(3TC)+ritonavir-boosted lopinavir(LPV/r),zidovudine(AZT)+3TC+efavirenz(EFV),and AZT+3TC+LPV/r,the incidence of dyslipidemia was lower in PLWH receiving TDF+3TC+EFV.In multivariate analysis,we found that the risks of elevations of TG,TC,and LDL-C were higher with TDF+3TC+LPV/r(TG:odds ratio[OR]=2.82,95%confidence interval[CI]:2.55-3.11,P<0.001;TC:OR=1.24,95%CI:1.14-1.35,P<0.001;LDL:OR=1.06,95%CI:1.00-1.12,P=0.041),AZT+3TC+EFV(TG:OR=1.41,95%CI:1.28-1.55,P<0.001;TC:OR=1.43,95%CI:1.31-1.56,P<0.001;LDL:OR=1.18,95%CI:1.12-1.25,P<0.001),and AZT+3TC+LPV/r(TG:OR=3.08,95%CI:2.65-3.59,P<0.001;TC:OR=2.40,95%CI:1.96-2.94,P<0.001;LDL:OR=1.52,95%CI:1.37-1.69,P<0.001)than with TDF+3TC+EFV,while treatment with TDF+3TC+LPV/r was less likely to restore HDL-C levels compared with TDF+3TC+EFV(OR=0.95,95%CI:0.92-0.97,P<0.001).In addition to antiretroviral regimens,antiretroviral therapy duration,older age,overweight,obesity and other traditional factors were also important risk factors for dyslipidemia.Conclusion:The incidence of dyslipidemia varies with different antiretroviral regimens,with TDF+3TC+EFV having lower risk for dyslipidemia than the other first-line free antiretroviral regimens in China. | Li-Qin Sun Jia-Ye Liu Yun He Yang Zhou Liu-Mei Xu Lu-Kun Zhang Fang Zhao Xiao-Ning Liu Ying Song Ting-Zhi Cao Yi-Mei Tian Man Rao Hui Wang | 2020 | Chinese Medical Journal2020，，23： | 9 |
| 8 | Diluted ferromagnetic semiconductor(LaCa)(ZnMn)SbO isostructural to “1111” type iron pnictide superconductors显示文摘We report discovery of ferromagnetism in(LaCa)(ZnMn)SbO isostructural to the well-studied iron-based superconductor LaFeAs(O1 xFx).Spin is induced by partial substitution of Mn2+for Zn2+,while charge is induced by substitution of Ca2+for La3+within the parent compound LaZnSbO.Ferromagnetism with Curie temperature(TC)is observed up to 40 K at the spin doping 0.15 by introducing Mn2+into the Zn2+sites for(La0.95Ca0.05)(Zn1 xMnx)SbO.The Hall coefficient measurement indicates p-type carrier for(La0.95Ca0.05)(Zn0.9Mn0.1)SbO with concentration of n^1020cm 3showing anomalous Hall effect below TC. | HAN Wei ZHAO Kan WANG XianCheng LIU QingQing NING FanLong DENG Zheng LIU Ying ZHU JinLong DING Cui MAN HuiYuan JIN ChangQing | 2013 | Science China(Physics,Mechanics & Astronomy)2013，56，11： | 8 |
| 9 | RNF2 is recruited by WASH to ubiquitinate AMBRA1 leading to downregulation of autophagy显示文摘洗(Wiskott-Aldrich 症候群蛋白质(黄蜂) 和疤相当或相同的事物) 被识别在经由 Arp2/3 激活排序的 endosomal 工作。我们以前表明了那洗在有 AMBRA1 的 BECN1-PIK3C3 建筑群是 BECN1 和现在的新 interactor。AMBRA1-DDB1-CUL4A 建筑群是为 BECN1 的连接 K63 的 ubiquitination 的 E3 ligase,它为导致饥饿的 autophagy 被要求。洗由 BECN1 ubiquitination 的抑制压制 autophagy。然而, AMBRA1 怎么在 autophagy 期间被调整,留下逃犯。这里,我们发现 RNF2 与 AMBRA1 联系经由 K48 连接充当 E3 ligase 到 ubiquitinate AMBRA1。RNF2 调停在离氨酸 45 点的 AMBRA1 的 ubiquitination。尤其是, RNF2 缺乏提高 autophagy 正式就职。在 autophagy 正式就职之上, RNF2 加强 AMBRA1 降级在的帮助下洗。洗缺乏与 AMBRA1 损害 RNF2 的协会阻碍 AMBRA1 降级。我们的调查结果揭示 autophagy 的规定的另一新奇的层通过为导致 autophagy 的 downregulation 的 AMBRA1 降级洗 RNF2 的招募。 | Pengyan Xia Shuo Wang Guanling Huang Ying Du Pingping Zhu Man Li Zusen Fan | 2014 | Cell Research2014，24，8： | 7 |
| 10 | Lactoferrin inhibits apoptosis through insulin-like growth factor I in primary rat osteoblasts显示文摘 | Jian-ming HOU En-yu CHEN Shi-chao WEI Fan LIN Qing-ming LIN Xu-hua LAN Ying XUE Man WU | 2014 | Acta Pharmacologica Sinica2014，35，4： | 6 |
| 11 | Contents of four active components in different commercial crude drugs and preparations of Danshen (Salvia miltiorrhiza)显示文摘AIM:To detect the contents of four active components of Salvia miltiorrhiza in various commercially available danshen crude drugs and preparations.METHODS:Commercially available danshen crude drugs from different sources,as well as danshen pills and intravenous injection preparations containing danshen alone or in combination with other herbs were collected.The composition of these danshen samples was analyzed using HPLC.Specifically,the amounts of magnesium tanshinoate B(MTB),danshensu,isotanshinone IIA,and cryptotanshinone were determined.In some of these samples,the content of MTB was further confirmed by liquid chromatography-tandem mass spectrometer (LC-MS)/MS method.RESULTS:There were great variations in the amount of the four active ingredients in the commercially available danshen crude drugs and drug preparations in this study.The amount of MTB was the highest among the four components measured in the crude drugs.However,the amounts of MTB in all danshen preparations were much lower than those in crude drugs.The 2 lipophilic components,isotanshinone IIA and cryptotanshinone,were very low or not detectable in both injection and oral preparations.CONCLUSION:MTB can be used to standardize the various forms of danshen crude drugs and drug preparations from different sources.In view of the variation in the amounts of MTB and other components,improvement in the production methods of danshen preparations is essential to ensure consistent amount of its active ingredients and reproducible pharmacological actions. | Susan Wai Sum LEUNG Yaw Loong SIOW Ricky Ying Keung MAN | 2002 | Acta Pharmacologica Sinica2002，23，12： | 6 |
| 12 | Eosinophil-derived CCL-6 impairs hematopoietic stem cell homeostasis显示文摘嗜曙红血球(曙光女神) 长在层次造血的系统被看作了结束阶段受动器房间。证据的众多的线建议了那个曙光女神是关于各种各样的煽动性或有免疫力的反应的开始,繁殖和规定的多功能的白血球,特别在过敏疾病。最近的研究证明了那个曙光女神也为骨头髓血浆房间的维护和 B 房间的区别被要求。然而,曙光女神是否贡献造血的干细胞(HSC ) 的规定,仍然保持不清楚动态平衡。这里,我们证明曙光女神由在野类型的老鼠损害 HSC 静止和体质能力破坏 HSC 动态平衡后面的 ovalbumin (卵) 挑战并且甚至由引起骨头髓 HSC 在 Cd3-Il-5 转基因的老鼠的失败和疲劳。HSC 的损害维护和功能与导致曙光女神的氧化还原作用不平衡(增加的氧化 phosphorylation 和减少的抗氧化剂层次) 被联系。用集体 spectrometry,更重要地,我们决定 CCL-6 在嗜曙红血球过多下面在高水平被表示。我们证明 CCL-6 为损害 HSC 动态平衡导出曙光女神、负责。有趣地,有特定的抵销抗体的 CCL-6 的阻塞,当在质问卵的老鼠加重嗜曙红血球过多航线发炎时,恢复了 HSC 的体质能力。因此,我们的学习在 HSC 动态平衡揭示 Eos/CCL-6 的意外功能。 | Chao Zhang, Fei Li, Xufei Du, Man Luo, Wen Hua, Wen Li, Zhihua Chen, Songmin Ying, Huahao Shen Weiwei Yi, Hu Wang, Zhenyu Ju Hu Wang, Zhenyu Ju Ping Wu, Chao Peng, Catherine CL Wong James J Lee Songmin Ying Songmin Ying Huahao Shen | 2018 | Cell Research2018，28，3： | 6 |
| 13 | Effect of devazepide reversed antagonism of CCK-8 against morphineon electrical and mechanical activities of rat duodenum in vitro显示文摘INTRODUCTIONCholecystokininoctapeptide(CCK8)isatypicalbraingutpeptide.ManydatashowthatCCK8hasbeenthestrongestendogenousant... | XU Man Ying 1, LU Hui Ming 1, WANG Shu Zhen 1, SHI Wen Yan 2, WANG Xin Chun 2, YANG Dong Xiao 3, YANG Chun Xiao 3 and YANG Li Zhuang 3 | 1998 | World Journal of Gastroenterology1998，4，6： | 4 |
| 14 | Non-genetic photoacoustic stimulation of single neurons by a tapered fiber optoacoustic emitter显示文摘Neuromodulation at high spatial resolution poses great significance in advancing fundamental knowledge in the field of neuroscience and offering novel clinical treatments.Here,we developed a tapered fiber optoacoustic emitter(TFOE)generating an ultrasound field with a high spatial precision of 39.6 pm,enabling optoacoustic activation of single neurons or subcellular structures,such as axons and dendrites.Temporally,a single acoustic pulse of sub-microsecond converted by the TFOE from a single laser pulse of 3 ns is shown as the shortest acoustic stimuli so far for successful neuron activation.The precise ultrasound generated by the TFOE enabled the integration of the optoacoustic stimulation with highly stable patch-clamp recording on single neurons.Direct measurements of the electrical response of single neurons to acoustic stimulation,which is difficult for conventional ultrasound stimulation,have been demonstrated.By coupling TFOE with ex vivo brain slice electrophysiology,we unveil cell-type-specific responses of excitatory and inhibitory neurons to acoustic stimulation.These results demonstrate that TFOE is a non-genetic single-cell and sub-cellular modulation technology,which could shed new insights into the mechanism of ultrasound neurostimulation. | Linli Shi Ying Jiang Fernando R.Fernandez Guo Chen Lu Lan Heng-Ye Man John A.White Ji-Xin Cheng Chen Yang | 2021 | Light(Science & Applications)2021，10，8： | 4 |
| 15 | Overview of the Shenzhen Emergency Medical Service Call Pattern显示文摘BACKGROUND:In Shenzhen,the Emergency Medical Service(EMS) system has been in service since 1997.This study aims to examine the operation of Shenzhen 120 EMS center and to identify the reasons of calling EMS.METHODS:In this retrospective quantitative descriptive study,the data from the Shenzhen 120 EMS registry in 2011 were analyzed.RESULTS:Shenzhen 120 EMS center is a communication command center.When the number of 120 are dialed,it is forwarded to the closest appropriate hospital for ambulance dispatch.In2011,the Shenzhen 120 EMS center received 153 160 ambulance calls,with an average of 420 calls per day.Calling emergency services was mainly due to traffic accidents.Trauma and other acute diseases constituted a majority of ambulance transports.The adult patients aged 15-60 years are the principal users of EMS.There are no recognized 'paramedic' doctors and nurses.The pre-hospital emergency service is under the operation of emergency departments of hospitals.Shenzhen at present does not have specialized pre-hospital training for doctors and nurses in posttrauma management.Moreover,specialized pre-hospital training,financial support,and public health education on proper use of EMS should be emphasized.CONCLUSION:The Shenzhen 120 EMS center has its own epidemiology characteristics.Traumatic injury and traffic accident are the main reasons for calling ambulance service.In-depth study emphasizing the distribution and characteristics of trauma patients is crucial to the future development of EMS. | Shuk Man Lo Yi Min Yu Lap Yip Larry Lee Mi Ling Eliza Wong Sek Ying Chair Edward J Kalinowski Tak Shing Jimmy Chan | 2012 | World Journal of Emergency Medicine2012，3，4： | 4 |
| 16 | Association between Fine Particulate Air Pollution and Outpatient Visits for Eczema in Beijing, China: A Time-series Analysis显示文摘Eczema is a very common inflammatory skin disease characterized by patches of erythematous,itchy, cracked, and scaly skin.According to the 2010 Global Burden of Disease Study, the global prevalence of eczema is 3.33% and the leading cause of skin condition-based disability-adjusted life years is eczema[1].The large health burden brought about by eczema suggests high direct medical costs and health care utilization[2].Thus, eczema prevention is of great importance in efforts to eliminate the public health burden of the disease. | WANG Xiao Wen TIAN Yao Hua CAO Ya Ying SONG Jing LI Man WU Yao WANG Meng Ying HUANG Zhe HU Yong Hua | 2019 | Biomedical and Environmental Sciences2019，32，8： | 4 |
| 17 | Could saline irrigation clear all residual common bile duct stones after lithotripsy?A self-controlled prospective cohort study显示文摘BACKGROUND A previous study showed that irrigation with 100 mL saline reduced residual common bile duct(CBD)stones,which potentially cause recurrent stones after endoscopic retrograde cholangiopancreatography.AIM To determine whether saline irrigation can improve CBD clearance after lithotripsy.METHODS This prospective self-controlled study enrolled patients receiving mechanical lithotripsy for large(>1.2 cm)CBD stones.After occlusion cholangiography confirmed CBD stone clearance,peroral cholangioscopy(POC)was performed to determine clearance scores based on the number of residual stones.The amounts of residual stones spotted via POC were graded on a 5-point scale(score 1,worst;score 5,best).Scores were documented after only stone removal(control)and after irrigation with 50 mL and 100 mL saline,respectively.The stone composition was analyzed using infrared spectroscopy.RESULTS Between October 2018 and January 2020,47 patients had CBD clearance scores of 2.4±1.1 without saline irrigation,3.5±0.7 with 50 mL irrigation,and 4.6±0.6 with 100 mL irrigation(P<0.001).Multivariate analysis showed that CBD diameter>15 mm[odds ratio(OR)=0.08,95%confidence interval(CI):0.01-0.49;P=0.007]and periampullary diverticula(PAD)(OR=6.51,95%CI:1.08-39.21;P=0.041)were independent risk factors for residual stones.Bilirubin pigment stones constituted the main residual stones found in patients with PAD(P=0.004).CONCLUSION Irrigation with 100 mL of saline may not clear all residual CBD stones after lithotripsy,especially in patients with PAD and/or a dilated(>15 mm)CBD.Pigment residual stones are soft and commonly found in patients with PAD.Additional saline irrigation may be required to remove retained stones. | Yan-Yan Lin Yu-Dong Wang Ping Yue Xian-Zhuo Zhang Joseph W Leung Pan-Pan Jiao Man Yang Hai-Ping Wang Bing Bai Ying Liu Jin-Duo Zhang Hong-Bo Chen Wen-Bo Meng Xun Li | 2021 | World Journal of Gastroenterology2021，27，4： | 4 |
| 18 | Morphological observation, RNA-Seq quantification, and expression profiling: novel insight into grafting-responsive carotenoid biosynthesis in watermelon grafted onto pumpkin rootstock显示文摘西瓜是在中国的重要、节俭的园艺的庄稼,在的地方 20% 植物是的 ~ grafted。grafted 西瓜水果的发展包含在水果导致动态变化的基因相互作用的一个多样的范围。然而,位于导致接枝的水果质量变化下面的分子的机制是不清楚的。在现在的学习,我们由高效的液体层析测量了 lycopene 内容并且使用了 RNA-Seq (quantification ) 从西瓜 grafted 执行水果的染色体宽的抄本分析到南瓜根茎上(南瓜接枝西瓜, PGW ) ,自我接枝西瓜(SGW ) ,和接枝得非的西瓜(NGW ) 。结果在 PGW 水果的肉在 lycopene 内容显示出变化,首先在四增加然后减少上演,它与在 NGW 和 SGW 水果的肉的趋势不同。介绍数据的 transcriptome 在水果生长和开发期间在 lycopene 生合成的导致接枝的基因规定上提供了新信息。从与 lycopene 生合成有关的 8 个基因家庭( GGPS , PSY , PDS , ZDS , CRTISO , LCYb , LCYe ,和 CHY )的 33 基因的表示层次,在水果着色法起关键作用并且显著地作出贡献到水果 phytonutrient 价值,在西瓜在水果开发的四个时期期间被监视。与那些相比 NGW 和 SGW, 14 基因是在水果开发期间在 PGW 表示的差别,建议这些基因可能帮助调停在 grafted 的 lycopene 生合成西瓜水果。我们的工作在 PGW 水果开发期间提供一些新奇卓见进接枝应答的类胡萝卜素新陈代谢和它的潜在的角色并且成熟。 | Guang Liu Xingping Yang Jinhua Xu Man Zhang Qian Hou Lingli Zhu Ying Huang Aisheng Xiong | 2017 | Acta Biochimica et Biophysica Sinica2017，49，3： | 3 |
| 19 | Consensus for prevention and management of coronavirus disease 2019 (COVID-19) for neurologists显示文摘Coronavirus disease 2019(COVID‐19)has become a pandemic disease globally.Although COVID-19 directly invades lungs,it also involves the nervous system.Therefore,patients with nervous system involvement as the presenting symptoms in the early stage of infection may easily be misdiagnosed and their treatment delayed.They become silent contagious sources or‘virus spreaders’.In order to help neurologists to better understand the occurrence,development and prognosis,we have developed this consensus of prevention and management of COVID‐19.It can also assist other healthcare providers to be familiar with and recognise COVID-19 in their evaluation of patients in the clinic and hospital environment. | Huijuan Jin Candong Hong Shengcai Chen Yifan Zhou Yong Wang Ling Mao Yanan Li Quanwei He Man Li Ying Su David Wang Longde Wang Bo Hu | 2020 | Stroke & Vascular Neurology2020，5，2： | 3 |
| 20 | Ecological Offsetting in China's Coastal Wetlands: Existing Challenges and Strategies for Future Improvement显示文摘Land-use changes in coastal wetlands have led to a worldwide degradation of marine coastal ecosystems and a loss of the ecological services they provide. Ecological offsetting is a popular strategy and an effective mitigation measure for ecosystems that have been degraded, damaged, or destroyed and is critical for habitats where natural recovery is hindered. However, the current understanding of the theory and practice of ecological offsetting for coastal wetlands is extremely limited in many developing countries. We conducted a review of ecological offsetting for coastal wetlands projects and studies in China in 1979–2017 to explore the application and limitations of ecological offsetting theory. It was found that China's coastal ecological offsetting regime has recently entered a rapidly developing stage, with an increasing number of different types of offsetting projects conducted, but theoretical research lags behind practical applications. Considerable governmental, social, technological and ethical challenges remain to resolve. Coastal ecological offsetting schemes have been inconsistent in meeting conservation objectives or preventing net losses because of the challenges of ensuring they are fully consistent in practice(mainly in-kind offsets) and theory(mainly out-of-kind offsets). Ecological offsetting projects were primarily implemented by government, developers, and non-profit organizations. The available funding of coastal ecological offsetting projects is insufficient, which makes ecological offsetting a risky operation. Therefore, we propose strategies for improvement that integrate the consideration of theoretical and practical challenges in the offsetting process, while providing a scientific basis and directional guidance for the future practice of biodiversity conservation and environmental management. | YU Shuling CUI Baoshan XIE Chengjie MA Xu MAN Ying NING Zhonghua | 2019 | Chinese Geographical Science2019，29，2： | 3 |
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