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| 1 | 极端嗜热菌海栖热袍菌木聚糖酶B的克隆和表达显示文摘极端嗜热菌海栖热袍菌MSB8(ThermotogamaritimaMSB8)能够利用木聚糖代谢 ,并具有两个产木聚糖酶的基因 .本研究首次克隆和在大肠杆菌中表达海栖热袍菌MSB8的第 2个木聚糖酶基因即xynB基因 .以基因组DNA为模板 ,根据xynB基因的全序列设计了两对引物 ,在合适的PCR反应条件下有效地扩增出了xynB基因片段 .选用pET2 8a(+)表达载体 ,NcoI-HindⅢ酶切位点并编码了羧基端 6×His标签的阳性克隆表达成可溶且具有生物活性的蛋白质 .xynB结构基因由 10 4 4对碱基组成 ,编码 347个氨基酸 .根据已知木聚糖酶蛋白质氨基酸的同源性分析 ,XynB与T .sp.strainFjSS3-B .1的XynA的同源性最大 ,为 85 % ,与T .neapolitana的XynB同源性次之 ,为 82 % ,与其它木聚糖酶的同源性小于 4 3% .另外 ,XynB属于F/ 10族木聚糖酶 ,且含有单一功能区 .表 3图 3参 | 江正强 李里特 林清 MOHAMMAD Mainul Ahsan | 2002 | 应用与环境生物学报2002，8，3： | 8 |
| 2 | 海栖热袍菌极端耐热木聚糖酶B的提纯显示文摘克隆并在大肠杆菌中表达的海栖热袍菌的 xyn B基因 ,其表达产物木聚糖酶 B的 C 末端带有 6×His标签 ,研究了这种基因重组酶的提纯方法。通过对粗酶提取液的热变性处理 ,Ni NTA亲和柱层析和离子柱层析 ,最终得到了电泳纯的木聚糖酶 B,提纯倍数 4 4.4 ,得率 11。SDS PAGE法测定木聚糖酶 B的相对分子质量为 4 2 ku,与理论推算值 4 2 | 江正强 李里特 林清 Mohammad Mainul Ahsan | 2001 | 中国农业大学学报2001，6，6： | 8 |
| 3 | 一类食饵种群中具有传染病的捕食-被捕食系统(英文)显示文摘对疾病仅在食饵种群传播的有比例依赖的捕食-被捕食系统的动力学进行了分析,给出了每个平衡点附近系统的性态,定义了决定疾病灭绝和成为地方病的阁值R_0.得出的结论是:在比例依赖的捕食-被捕食系统中,染病食饵种群可以充当一个生物控制量,以抑制种群的绝灭. | Mainul Haque Joydev Chattopadhyay | 2007 | 生物数学学报2007，22，1： | 4 |
| 4 | Semi-continuous casting of magnesium alloy AZ91 using a filtered melt delivery system显示文摘A 3-D numerical simulation of an industrial-sized slab caster for magnesium alloy AZ91 has been carried out for the steady state operational phase of the caster.The simulated model consists of an open-top melt delivery system fitted with a porous filter near the hot-top.The melt flow through the porous filter was modeled on the basis of Brinkmann-Forchimier-Extended non-Darcy model for turbulent flow.An in-house 3-D CFD code was modified to account for the melt flow through the porous filter.Results are obtained for four casting speeds namely,40,60,80,and 100 mm/min.The metal-mold contact region as well as the convective heat transfer coefficient at the mold wall were also varied.In addition to the above,the Darcy number for the porous media was also changed.All parametric studies were performed for a fixed inlet melt superheat of 64℃.The results are presented pictorially in the form of temperature and velocity fields.The sump depth,mushy region thickness,solid shell thickness at the exit of the mold and axial temperature profiles are also presented and correlated with the casting speed through regression analysis.©2015 Production and hosting by Elsevier B.V.on behalf of Chongqing University. | Mainul Hasan Latifa Begum | 2015 | Journal of Magnesium and Alloys2015，3，4： | 2 |
| 5 | Endoscopic third ventriculostomy in children:problems and surgical outcome:analysis of 34 cases显示文摘Background:Endoscopic third ventriculostomy(ETV)has been established as a viable treatment option for obstructive hydrocephalus of children over 6 weeks of age.ETV in pediatric groups may be unsuccessful due to the failure of absorption of cerebrospinal fluid(CSF)or reclosure of ventriculostomy stoma or due to infection.The exact cause is still debatable.Some issues like failure to eliminate the second membrane during the procedure or formation of the new arachnoid membrane at the stoma are still not clear.This study aims to assess the surgical failure of ETV and its predisposing factors.Methods:Thirty-four pediatric patients with hydrocephalus were analyzed retrospectively.The patients’age limit was between 2.5 months and 14 years.This is a retrospective study of 34 patients in a single private hospital between June 2012 and January 2018.Patients having hydrocephalus in pediatric groups more than 6 weeks of age were included in the study.Results:The mean age of all patients was 51.25±53.90 months and the mean follow-up period was 50.47±20.84 months.Of 34 surgeries,the success rate was 79%and the failure rate was 21%.Within 2 years,the success rate was 68.42%and above 2 years’success rate was 93.33%.In this series,7 cases of ETV were re-explored and found ventriculostomy stoma closure in 3 cases,the presence of the second membrane in re-exploration 2 cases,and presence of inflammatory arachnoid membrane in re-exploration 2 cases.The use of dexamethasone around the stoma in inflammatory stoma was useful,having no recurrence.In one patient of the second membrane probably due to absorption failure in communicating hydrocephalus re-exploration was failed and was managed successfully with VP shunt.Conclusions:Predisposing factors causing ETV failure are ventriculostomy stoma closure by new arachnoid granulation tissues,remnants of the second membrane inside the stoma,CSF absorption failure,infection/high protein in CSF and inappropriate patient selection. | Md Moshiur Rahman S.I.M.Khairun Nabi Khan Robert Ahmed Khan Rokibul Islam Mainul Haque Sarker | 2021 | Chinese Neurosurgical Journal2021，7，2： | 2 |
| 6 | Characterization of a Thermostable Family 10 Endo-Xylanas (XynB) from Thermotoga maritime that cleaves p-nitropheny-β-D-xyloside显示文摘 | Jiang Zhengqiang Atsushi Kobayashi Mohammad Mainul Ahsan | 2001 | Journal of Bioscience and Bioengineering2001，2，5： | 1 |
| 7 | Effectiveness of using fibre-reinforced polymer composites for underwater steel pipeline repairs显示文摘 | Md Shamsuddoha Md Mainul Islam Thiru Aravinthan Allan Manalo Kin-tak Lau | 2013 | Composite Structures2013，，： | 1 |
| 8 | X-ray enabled detection and eradication of circulating tumor cells with nanoparticles显示文摘 | Mainul Hossain Yang Luo Zhaoyong Sun Chaoming Wang Minghui Zhang Hanyu Fu Yong Qiao Ming Su | 2012 | Biosensors and Bioelectronics2012，，1： | 1 |
| 9 | Selective Fluorimetric Recognition of Cesium Ion by 15-Crown-5-Anthracene显示文摘 | Hyo Sook Seo Mohammad Mainul Karim Sang Hak Lee | 2008 | Journal of Fluorescence2008，，5： | 1 |
| 10 | Higher or der ionospheric propagation effects on GPS radio oc- cultation signals显示文摘 | MAINUL HOQUE M JAKOWSKI N | 2010 | Advances in Space Research2010，，： | 1 |
| 11 | Influence of nitrogen and iron limitation on lipid production by crytococcus curvatus grown in batch and fed-batch culture 显示文摘 | Philippe JB Louis-Marie Granger | 1996 | Process Biochemistry1996，31，4： | 1 |
| 12 | Ratio-dependent predator–prey model of interacting population with delay effect显示文摘 | Sahabuddin Sarwardi Mainul Haque Prashanta Mandal | 2012 | Nonlinear Dynamics2012，，3： | 1 |
| 13 | In vitro cytotoxicity of surface modified bismuth nanoparticles显示文摘 | Yang Luo Chaoming Wang Yong Qiao Mainul Hossain Liyuan Ma Ming Su | 2012 | Journal of Materials Science: Materials in Medicine2012，，10： | 1 |
| 14 | Higher order ionospheric effects in precise GNSS positioning 显示文摘 | Mainul Hoque M and Jakowski N | 2007 | Original Article2007，81，： | 1 |
| 15 | Physical and Mathematical Modeling of Inert Gas-Shrouded Ladle Nozzles and Their Role on Slag Behavior and Fluid Flow Patterns in a Delta-Shaped, Four- Strand Tundish 显示文摘 | Kinnor C Mainul H Mihaiela I | 2010 | Metallurgical and Materials Transactions B2010，41，： | 1 |
| 16 | A Pinch or apint evidence of pesticide overuse in bangladesh显示文摘 | SUSMITA DASGUPTA CRAIG MEISNER MAINUL HUQ | 2007 | Journal of Agricultur-al Economics2007，58，1： | 1 |
| 17 | Influence of nitrogen and ironlimitation on lipid production by crytococcus curvatus grown inbatch and fed-batch culture显示文摘 | MAINUL H PHILIPPE J B LOUIS M G | 1996 | Process Biochemistry1996，31，4： | 1 |
| 18 | Influence of nitrogen and iron limitation on lipid production by crytococcus curvatus grown in batch and fed-batch culture显示文摘 | MainuL Hassan Philippe J Blanc Louis Mar Granger | 1996 | Process Biochemistry1996，31，4： | 1 |
| 19 | Effect of pollutants on the ergosterol content as indicator of fungal biomass 显示文摘 | Martha BA Mainul H Raunel T | 2002 | J Microbiol Methods2002，50，12： | 1 |
| 20 | Characterization of The mosttalbe Family 10 Endo-Xylanas(XynB)from Thermotoga maritime That Cleaves p-Nitropheny-β-D-Xyloside显示文摘 | Jiang zheng qiang Atsushi Kobayashi Mohammed Mainul Ahsan | 2001 | Journal of Bioscience and Bioengingineering2001，2，5： | 1 |
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