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| 1 | Genetic improvement of Trichoderma ability to induce systemic resistance显示文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity. | Ciliento R Woo S L Di Benedetto P Ruocco M Scala F Soriente I Ferraioli S Brunner K Zeilinger S Mach R L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004,30,4: | 2 |
| 2 | Comparative sequence analysis of human cytomegalovirus strains 显示文摘 | Lehner R Stamrninger T Mach M | 1991 | J Clin Microbiol1991,29,11: | 1 |
| 3 | Lightning optical pulse statistics from storm overflights during the altus cumulus electrification study显示文摘 | Mach D M Blackeslee R J Bailey J C | 2005 | Atmospheric Research2005,76,14: | 1 |
| 4 | Neurophysiological correlates in interface design: An HCI perspective显示文摘 | Mach Q H Hunter M D Grewal R S | 2010 | Com- puters in Human Behavior2010,26,: | 1 |
| 5 | The sigm arecturn pouch( Mainz Pouch Ⅱ) clinical after 3 years of clinical experience显示文摘 | Fisch M Kinkowskills M Wam mach R | 1995 | J Urol1995,153,: | 1 |
| 6 | Highly efficient all-nitride phosphor-converted white light emitting diode显示文摘 | Mueller Mach R Mueller G Krames M R | 2005 | Phys Status Solidi A2005,202,9: | 1 |
| 7 | Xyr1 receives the lactose induction signal and regulates lactose metabolism in Hypocrea jecorina显示文摘 | Stricker A R Steiger M G Mach R L | | 0,,21: | 1 |
| 8 | Transformation of Trichoderma reesei based on hygromycin B resistance using homologous expression signals显示文摘 | MACH R L SCHINDLER M KUBICEK C P | 1994 | Curr Genet1994,25,6: | 1 |
| 9 | Site errors and detection efficiency in a magnetic direction-finder network for location lightning strikes to ground显示文摘 | MACH D M MACGORMAN D R RUST W D | 1986 | J Atmos Oceanic Tech1986,21,3: | 1 |
| 10 | The assoeiation of serum anticholinergie activity with delirium in elderly medical patients显示文摘 | FLACKER J M CUMMINGS V MACH J R Jr | 1998 | Am J Geriatr Psychiatry1998,6,1: | 1 |
| 11 | White light emitting diodes for illumination 显示文摘 | Mach R M Mueller G O | 2000 | SPIE2000,3938,: | 1 |
| 12 | Nucleotide sequence and expression of a novel glycine-rich protein gene from Arabidopsis thaliana显示文摘 | Quigley F Villiot M L Mache R | 1991 | Plant Mol Biol1991,17,: | 1 |
| 13 | The two major xylanases from Trichoderma reesei: characterization of both enzymes and genes显示文摘 | Trrnen A Mach R L Messner R Gonzalea R Kalkkinen N Harkki A M and Kubicek C P | 1992 | Bio/technology1992,10,: | 1 |
| 14 | Quantitative phase tomography of Arabidopsis seeds reveals intercellular void network显示文摘 | Cloetens P Mache R Schlenker M | 2006 | Proceedings of National Academy of Sciences of the United States of America2006,103,14: | 1 |
| 15 | Different inducibility of espression of the two xylanase genes xyn1 and xyn2 in Trichoderma reesei显示文摘 | Zeilinger S Mach R L Schindler M Herzog P Kubicek C P | 1996 | J of Biological Chemistry1996,271,25: | 1 |
| 16 | Effects on digestibility and growth of juvenile cobia (Rachycentron canadum) fed fish or crab silage protein显示文摘 | Mach D T N Nguyen M D Nortvedt R | 2010 | Aquaculture Nutrition2010,16,3: | 1 |
| 17 | The Arabidopsis accelerated cell death gene aed2 encodes red chlorophyll catabolite reductase and suppresses the spread of disease symptoms 显示文摘 | Mach J M Castillo A R Hoogstraten R | 2001 | Proc Natl Acad Sci USA2001,,265: | 1 |
| 18 | The Arabidopsis-accelerated cell death gene ACD2 encodes red chlorophyll catabolite reductase and suppresses the spread of disease symptoms显示文摘 | Mach J M Castillo A R Hoogstraten R | 2001 | Proc Natl Acad Sci USA2001,98,2: | 1 |
| 19 | Comparative sequence analysis of human cytomegalovirus strains显示文摘 | Lehner R Stamminger T Mach M | 1991 | J Clinical Microbiology1991,29,: | 1 |
| 20 | The Arabidopsis-accelerated cell death gene ACD2 encodes red chlorophyll catabolite reductase and suppresses the spread of disease symptoms显示文摘 | Mach J M Castillo A R Hoogstraten R | 2001 | Proceedings of the National Academy of Sciences2001,98,2: | 1 |