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| 1 | Genetic improvement of Trichoderma ability to induce systemic resistance显示文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity. | Ciliento R Woo S L Di Benedetto P Ruocco M Scala F Soriente I Ferraioli S Brunner K Zeilinger S Mach R L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 2 | CCR9 is a homing receptor for plasmacytoid dendritic cells to the small intestine显示文摘 | Wendland M Czeloth N Mach N | 2007 | Proc Natl Acad Sci USA2007，104，15： | 1 |
| 3 | An ELISA using recombinant proteins for the detection of neutralizing antibodies against human cytomegalovirus显示文摘 | KROPFF B LANDINI M P MACH M | 1993 | Journal of Medical Virology1993，39，3： | 1 |
| 4 | Osmoregulatory isoform of dihydroxyacetone phosphate reductase from Dunaliella tertiolecta:purification and characterization显示文摘 | Ghoshal D Mach D Agarwal M | 2002 | Protein Expr Purif2002，24，： | 1 |
| 5 | Different tumors in bone each give rise to a distinct pattern of skeletal destruction, bone cancer-related pain behaviors and neuro chemical changes in the central nervous system 显示文摘 | Sabino M A Luger NM Mach DB | 2003 | Int J Cancer2003，104，5： | 1 |
| 6 | Comparative sequence analysis of human cytomegalovirus strains 显示文摘 | Lehner R Stamrninger T Mach M | 1991 | J Clin Microbiol1991，29，11： | 1 |
| 7 | Autonomic nervous system activity in constipation-predominant irritable bowel syndrome patients显示文摘 | Mazur M Mach T Thor P | | 0，，08： | 1 |
| 8 | Two-dimensional speed and optical risetime estimates for natural and triggered dart leaders显示文摘 | Mach D M Rust W D | 1997 | J Geophys Res1997，102，13： | 1 |
| 9 | Photoelectric return-stroke velocity and peak current estimates in natural and triggered lightning显示文摘 | Mach D M Rust W D | 1989 | J Geophys Res1989，94，13： | 1 |
| 10 | Estimation of Drug-Protein Binding Parameters on Assuming the Validity of Thermodynamic Equilibrium显示文摘 | Soltes L Mach M | 2002 | Journal of Chromatography B2002，768，1： | 1 |
| 11 | The differential effect of team members' trust on team performance: The me- diation role of team cohesion显示文摘 | Mach M Dolan S Tzafrir S | 2010 | Journal of Occupa- tional and Organizational Psychology2010，83，3： | 1 |
| 12 | Chronic disease management for depression in primary care:a summary of the current literature and implications for practice 显示文摘 | Kates N Mach M | 2007 | Can J Psychiatry2007，52，2： | 1 |
| 13 | Lightning optical pulse statistics from storm overflights during the altus cumulus electrification study显示文摘 | Mach D M Blackeslee R J Bailey J C | 2005 | Atmospheric Research2005，76，14： | 1 |
| 14 | Human cytomegalovirus infection elicits a glycoprotein M ( gM )/gN-specific virus-neutralizing antibody response显示文摘 | Shimamura M Mach M Britt WJ | 2006 | J Virol2006，80，9： | 1 |
| 15 | Neurophysiological correlates in interface design: An HCI perspective显示文摘 | Mach Q H Hunter M D Grewal R S | 2010 | Com- puters in Human Behavior2010，26，： | 1 |
| 16 | The sigm arecturn pouch( Mainz Pouch Ⅱ) clinical after 3 years of clinical experience显示文摘 | Fisch M Kinkowskills M Wam mach R | 1995 | J Urol1995，153，： | 1 |
| 17 | Osmoregulatory isofunn of dihydroxyacetone phosphate reduetase from Dunaliella teriolecta: purification and characterization显示文摘 | Ghoshal D Mach D Agarwal M | 2002 | Protein Expr Purif2002，24，： | 1 |
| 18 | Increasing the amount of n-3 fatty acid in meat from young hostein bulls through nutrition显示文摘 | Mach N Devant M Diaz I | 2006 | J Animal Sci2006，84，： | 1 |
| 19 | Distribution of linear antigenic sites on glycoprotein gp55 of human cytomegalovirus 显示文摘 | Kniess N Mach M Fay J | 1991 | J Virol1991，65，1： | 1 |
| 20 | Are probi- otics effective in the treatment of fungal colonization of the gastro- intestinal tract Experimental and clinical studies 显示文摘 | Zwolinska Wcisko M Brzozowski T Mach T | 2006 | J Physiol Pharmacol2006，57，9： | 1 |
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